Uji Aktivitas Antioksidan Sediaan Masker Hidrogel Ekstrak Etanol Teh Hijau (Camellia sinensis) Dengan Metode BCB

2021 ◽  
Vol 1 ◽  
pp. 1059-1070
Author(s):  
Asri Septiani ◽  
W Wirasti ◽  
S Slamet ◽  
Urmatul Waznah
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Color Change ◽  
Ethanol Extract ◽  
Test Method ◽  
Stability Test ◽  
Cycling Test ◽  
The Stability ◽  
Β Carotene

AbstractGreen tea is a plant that contains antioxidants. The content of green tea compounds that act as antioxidants are polyphenols. The addition of natural antioxidants in hydrogel masks has the potential as an alternative to synthetic antioxidants. The purpose of this study was to determine the antioxidant activity of the hydrogel mask preparation of green tea (Camellia sinensis L.) ethanol extract. The method of determining antioxidant activity was carried out by the -Carotene Bleaching (BCB) method. The stability test of the preparation using the cycling test method was carried out for 6 cycles including organoleptic, viscosity, pH, homogeneity tests. The data obtained from the antioxidant activity test is the IC50 value. The IC50 results of the green tea ethanol extract were 27,162 ppm, while the IC50 values for the formulations 1, 2 and 3 were 40,893 ppm, respectively; 35,348 ppm; and 32,270 ppm. The results of the stability test showed that the preparation was stable from the parameters of viscosity, pH and homogeneity but not stable from the organoleptic parameters because there was a color change in the preparation containing the extract after the cycling test process. In conclusion, both extracts and hydrogel mask preparations contain high antioxidants with relatively stable dosage forms.Keywords: Green Tea, Antioxidants, Hydrogel Mask, -Carotene Bleaching (BCB), IC50. AbstrakTeh hijau merupakan salah satu tanaman yang mengandung antioksidan. Kandungan senyawa teh hijau yang berperan sebagai antioksidan adalah polifenol. Penambahan antioksidan alami pada masker hidrogel berpotensi sebagai alternatif pengganti antioksidan sintetis. Tujuan penelitian ini adalah mengetahui aktivitas antioksidan sediaan masker hidrogel ekstrak etanol teh hijau (Camellia sinensis L.). Metode penentuan aktivitas antioksidan dilakukan dengan metode β-Carotene Bleaching (BCB). Pengujian stabilitas sediaan dengan metode cycling test dilakukan sebanyak 6 siklus meliputi uji organoleptik, viskositas, pH, homogenitas. Data yang diperoleh dari uji aktivitas antioksidan berupa nilai IC50. Hasil IC50 ekstrak etanol teh hijau sebesar 27,162 ppm, sedangkan pada sediaan menunjukan nilai IC50 berturut-turut dari formulasi 1, 2 dan 3 sebesar 40,893 ppm; 35,348 ppm; dan 32,270 ppm. Hasil uji stabilitas menunjukkan sediaan stabil dari parameter viskositas, pH dan homogenitas tetapi tidak stabil dari parameter organoleptik karena terjadi perubahan warna pada sediaan yang mengandung ekstrak setelah proses cycling test. Kesimpulannya ekstrak maupun sediaan masker hidrogel mengandung antioksidan tinggi dengan bentuk sediaan relatif stabil.Kata kunci:Teh Hijau; Antioksidan; Masker Hidrogel; β-Carotene Bleaching (BCB);IC50.

scholarly journals Optimization of HIB Value Combination of Tween 60 and Span 80 on Cream Formulation of Ethanol Extract of Green Tea Leaves (Camellia Sinensis L.)

2018 ◽  
Vol 23 (3) ◽  
pp. 124
Author(s):  
Fransiska Lisa Anindya Putri ◽  
Akhmad Kharis Nugroho ◽  
Erna Prawita Setyowati
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Lattice Design ◽  
Dpph Method ◽  
Green Tea Leaves ◽  
Span 80 ◽  
Tween 60

Green tea (Camellia sinensis L.) is known to have ability to protect skin against free radicals. This is supported by polyphenol compound catechin. This research aims to determine the optimum Hydrophilic-Lipophilic Balance (HLB) value of Tween 60 and Span 80 compositions on the optimum cream formula of ethanol extract of green tea leaves. Tea leaves are extracted by macerating using 70% ethanol. Catechin in extract is known from Thin Layer Chromatography (TLC) test with silica gel 60 F254 as stationary phase and ethyl acetate:aquadest:formic acid (18:1:1 v/v) as mobile phase. Antioxidant activity is determined by 2,2-Diphenyl-1-picryhydrazyl (DPPH) method and value of Inhibition Concentration 50% (IC50) is then calculated. Formula optimization using Design Expert® version 7.1.5 (DX 7) software, Simplex Lattice Design (SLD) method with two components Tween 60 and Span 80. Cream is characterized according to physical properties organoleptic, homogeneity, viscosity, pH, spreadability, adhesiveness, and cream type. The optimum formula obtained is then tested for physical stability for 4 weeks at room temperature (28±2°C) and data are statistically analyzed using one-way ANOVA. The extract contains catechin proved with Retention factor (Rf) value 0.8 and has antioxidant activity with IC50 value 56.35 ppm. 6.4% Tween 60 and 3.6% Span 80 result an optimum HLB value 11.1. It has viscosity 2897.50±35.94 mPa.s, spreadability 18.44±0.06 cm2, adhesiveness 0.85±0.05 seconds, and pH 4.530±0.002. Statistical test shows that the cream is significantly altered at pH, but does not significantly change in viscosity, spreadability, and adhesiveness after being stored for 4 weeks.

scholarly journals EVALUASI SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 119-128
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Strong Activity ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves

Abstract Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

scholarly journals UJI AKTIVITAS SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 129-134
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Strong Activity ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves

Abstract   Green tea (Camellia sinensis L.) has the main efficacy as an antioxidant and contains polyphenol compounds in green tea leaves with the main component of polyphenol fraction namely Epigallocatechin-3-gallate (EGCG) which has strong activity to prevent free radicals that cause premature aging. Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

scholarly journals Peel Off Gel Mask containing Green Tea Leaf Extract (Camellia sinesis L) with Antioxidant Activity

2021 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Anis Yohana Chaerunisaa ◽  
Emma Surachman ◽  
Zila Khuzaimah
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ph Value ◽  
Tea Leaves ◽  
Drying Time ◽  
Dpph Method ◽  
Ic50 Values ◽  
Green Tea Leaves ◽  
The Stability

Peel off mask has been very popular and preferred due to its ease in application. Gel base has been preferred for its cold and moist sensation during application. When herbal ingredients such as extract is  introduced into gel formulation, the stability then will become an issue. The aim of the study was to formulate the peel-off mask gels containing green tea leaves extract (Camellia sinensis L.) which effective, stable, and safe to be used. The antioxidant study was conducted by using  Diphenylhydrazylpicryl (DPPH) method. The peel-off mask gel formula were Hidroxypropyl Methylcelulosa (HPMC) as gel base at 0.5% concentration and plasticizer Polyvinyl Alcohol (PVA) at concentration of 12%. Evaluation of peel-off mask gel included observation on pH, viscosity, dispersing power, and drying time during the 90 days of storage at room temperature. The IC50 values of the green tea (Camellia sinensis L.) extract was 5.511 ppm. The concentration of green tea leaves extract in the preparation were 1x IC50 (F1); 2x IC50 (F2)  and 3x IC50 (F3). The result showed that all preparations were stable in the aspect of consistency, color, and odor. Viscosity, drying time and dispersing power remained unchanged after 90 days of storage. The pH value changed, but still in the range of acceptable pH of topical preparations. The results of antioxidant activity study showed that formula F3 (peel-off mask gels with 3 x IC50 of green tea extracts) had the highest antioxidant activity.Keywords: antioxidant, Camellia sinensis L., peel off gel masks, green tea

scholarly journals FORMULASI SEDIAAN MASKER ANTIOKSIDAN DARI EKSTRAK TEH HIJAU (CAMELLIA SINENSIS)

2019 ◽  
Vol 10 (2) ◽  
pp. 109-118
Author(s):  
Desi Nawangsari
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanol Extract ◽  
Tea Leaves ◽  
Reduction Activity ◽  
Radical Reduction ◽  
Green Tea Leaves ◽  
Tea Leaf

Abstract   Research has been carried out on the formulation of maskantioxidant preparations containing green tea leaf extract. The study begins with testing the antioxidant activity of ethanol extract of green tea leaves with a free radical reduction method of 1,1-diphenyl-2-picrylhydrazyl using visible spectrophotometry. Testing of antioxidant activity showed that the ethanol extract of green tea leaves gave an IC50 value of 3.17µg / mL. Formulations of gel masks made were F0 (base without active substances), F1 (base + concentration of ethanol extract of green tea leaves for IC50 (0,000317%)), F2 (base + concentration of ethanol extract of green tea leaves for 50xIC50 (0, 0158%)), F3 (base + concentration of ethanol extract of green tea leaves for 100xIC50 (0.0317%)), F4 (base + concentration of ethanol extract of green tea leaves for IC50), F5 (base + concentration of ethanol extract of green tea leaves worth IC50), F6 (base + concentration of ethanol extract of green tea leaves for IC50. Evaluation of gel mask preparations include, organoleptic examination and homogeneity, pH, viscosity, dry time of preparation and testing of antioxidant stability. F2 provides better and higher free radical reduction activity than products on the market. F4, F5, F6 shows an increase in free radical reduction activity.   Keywords: Green tea leaves (Camellia sinensis L.), DPPh, IC50 gel mask.

Formulation and Stability Test of Lip Cream Preparation of Kenop Flower Ethanol Extract (Gomphrena globosa L.)

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Mevi Irianti Tonapa ◽  
Rani Dewi Pratiwi ◽  
Elsye Gunawan
Keyword(s):  
Ph Value ◽  
Ethanol Extract ◽  
Stability Test ◽  
Gomphrena Globosa ◽  
Homogeneous Composition ◽  
Semi Solid ◽  
The Stability ◽  
Ph Range ◽  
Average Dispersion ◽  
Brown Color

Kenop Flower (Gomphrena globosa L.) is used in the manufacture of lip cream because contains betacyanin pigments that function as color pigments. This study aims to determine the physical quality and stability of the lip cream preparation of the ethanol extract of kenop flower (Gomphrena globosa L.). This research was conducted experimentally, including the manufacture of lip cream formulations with ethanol extract of kenop flower (Gomphrena globosa L.) with a concentration of 10%. The results of the physical examination test for lip cream preparations for all preparations have a distinctive vanilla aroma with a semi-solid texture, F0 has ivory white color and F1-F3 has a brown color. The preparations had a homogeneous composition, average pH 6-7, had good greasing power, 5.0-5.8 average dispersion and 60.33-66.67 seconds average adhesion. The stability test carried out on day 28 found that all preparations were stable, had a distinctive vanilla aroma with a semi-solid texture, F0 had ivory white color and F1-F3 had a brown color. The preparation has a homogeneous composition; the average pH is 6-7. Where the lip cream formulas F1 and F3 decreased the pH value on the 28th day from 7 to 6 but tended to be stable and in the pH range that matched the lip pH. And there is no phase separation in all formulas.

Thermal Effects on the Stability and Antioxidant Activity of an Acid Polysaccharide Conjugate Derived from Green Tea

2009 ◽  
Vol 57 (13) ◽  
pp. 5795-5798 ◽  
Author(s):  
Xiaoqiang Chen ◽  
Yang Ye ◽  
Hao Cheng ◽  
Yongwen Jiang ◽  
Yalin Wu
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Thermal Effects ◽  
Acid Polysaccharide ◽  
The Stability

scholarly journals Antioxidant Activity of Ethanolic Extract and Various Fractions from Green Tea (Camellia sinensis L.) Leaves

2019 ◽  
Vol 11 (4) ◽  
pp. 771-776
Author(s):  
Karmika Indarti ◽  
Elsa Fitria Apriani ◽  
Agung Eru Wibowo ◽  
Partomuan Simanjuntak
Keyword(s):  
Antioxidant Activity ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Ethanolic Extract

scholarly journals AKTIVITAS ANALGESIK EKSTRAK ETANOL BULU BABI (ECHINOMETRA MATHAEI) PADA MENCIT PUTIH JANTAN

2020 ◽  
Vol 1 (02) ◽  
pp. 7
Author(s):  
Lestiono Lestiono ◽  
Angelica Kresnamurti
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Ethanol Extract ◽  
Negative Control ◽  
Test Method ◽  
Flavonoid Compound ◽  
Writhing Test ◽  
Peripheral Pain ◽  
Echinometra Mathaei

Sea urchin (Echinometra mathaei) is a potential marine resourches that had not been explored widely. It has flavonoid, triterpenoid, alkaloid, saponin compound with high antioxidant activity. Antioxidant activity and flavonoid compound were potentially correlated with analgesic activity. This study aimed  to examine e the potential of Echinometra mathaei as an analgesic in white male mice that were induced by acetic acid to produce peripheral pain in vivo (streatching in mice) with writhing test method. The amount of stretching was observed for 1 hour after administration of ethanol extract of Echinometra mathaei orally. Balb/c white mice which devided into 6 groups randomlly, as negative control group, a group that induced acetic acid 1%,  Asetosal group with dose of 65 mg/kgBW, and Echinometra Mathaei groups with the dose of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW. The results of this study showed that all dosage of Echinometra mathaei decrease the peripheral compared to negative control (P,0.05). The percentage inhibition of pain  at 200 mg/kgBW (72.59%) was the highest among other doses was significant different from asetosal group (52.53%). This research concluded that Echinometra mathaei has the analgesic activity to inhibit peripheral pain .    Keywords: Echinometra mathaei, ethanol extract, analgesic, mice, writhing test

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