scholarly journals Uji Formulasi dan Uji Efektivitas Repelan Lotion Ekstrak Daun Tembakau (nicotiana tabacum. L) Terhadap Nyamuk Aedes Aegypti

2021 ◽  
Vol 1 ◽  
pp. 1968-1976
Author(s):  
Rojiah Batsah ◽  
W Wirasti ◽  
F Fitriyani

AbstractTobacco leaves (Nicotiana tabaccum. L) have great potential as a pesticide or insect repellent, especially the Aedes aegypti mosquito. Maceration method was used to obtain tobacco leaf extract as much as 127.86 grams from the dried simplicia of tobacco leaves (Nicotiana tabaccum. L) 827 grams (yield 15.46%). The tobacco extract was made in the form of lotio with a concentration of 1%, 2%, and 3%, having repellant effectiveness of 90%, 96% and 100%, respectively. Physical stability test was carried out on the lotion 0%, 1%, 2%, and 3% as follows; organoleptic test (brownish color, typical tobacco aroma and homogeneity), spreadability test (6.1cm; 6.1cm; 6.2cm; 6.4cm), adhesion test (3.06sec; 2.8sec; 2.75sec; 2.61sec), cycling test (brownish color, thick consistency, homogeneity, and distinctive aroma of tobacco), pH test (7; 7; 7; 6,3), and viscosity test (4.708cPs, 4.575cPs, 4.465cPs, 4.403cPs). The results of One Way ANOVA analysis showed that there was a significant difference in each concentration of tobacco leaf extract lotio and Tukey's test showed that the repellant ability of 3% concentration had 100% percent repelance. It can be concluded that the ethanol extract lotion of tobacco leaves (Nicotiana tabaccum. L) has repellant effectiveness.Keywords: tobacco leaves, extract, lotion, repellant, ANOVA AbstrakDaun tembakau (Nicotiana tabaccum. L) memiliki potensi besar sebagai pestisida atau pengusir serangga khususnya nyamuk Aedes aegypti. Metode maserasi digunakan untuk mendapatkan ekstrak daun tembakau sebanyak 127,86 gram dari simplisia kering daun tembakau (Nicotiana tabaccum. L) 827 gram (rendemen 15,46%). Ekstrak tembakau dibuat dalam bentuk lotio dengan konsentrasi 1%, 2%, dan 3% memiliki efektivitas repelan berturut-turut adalah 90%, 96% dan 100%. Dilakukan uji stabilitas fisik terhadap lotio 0%,1%, 2%, dan 3% sebagai berikut; uji organoleptis (warna kecoklatan, aroma khas tembakau dan homogen), uji daya sebar (6,1cm; 6,1cm; 6,2cm; 6,4cm), uji daya lekat (3,06detik; 2,8detik; 2,75detik; 2,61detik), uji cycling test (warna kecoklatan, konsistensi kental, homogen, dan aroma khas tembakau), uji pH (7;7;7;6,3), dan uji viskositas (4.708cPs, 4.575cPs, 4.465cPs, 4.403cPs). Hasil analisis One Way ANOVA menunjukkan adanya perbedaan yang signifikan pada setiap konsentrasi lotio ekstrak daun tembakau dan uji Tukey menunjukkan kemampuan repelan konsentrasi 3% memiliki persen repelansi 100%. Hal ini dapat disimpulkan bahwa lotio ekstrak etanol daun tembakau (Nicotiana tabaccum. L) memiliki efektivitas repelan.Kata kunci: : daun tembakau; ekstrak; lotion; repelan; ANOVA

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 261
Author(s):  
Meyla C. M. Pratasik ◽  
Paulina V. Y. Yamlean ◽  
Weny I Wiyono

ABSTRACT Sesewanua Leaves (Clerodendron squamatum Vahl.) have the potential to be made as skin care cosmetic preparations but must be in the right formulation to achieve the desired effect. The aim of this study was to determine the physical stability of a cream from Sesewanua leaf extract. This study used a experimental method by making ethanol extract of Sesewanua leaves with a concentration of 0.5% and physical stability test. Physical evaluation was carried out before and after cycling test through organoleptic observation, homogenety test, pH test, dispersion test, adhesion test, viscosity test, centrifugation test and cream type determination. The results of the physical evaluation showed that F1 without Sesewanua leaf extract and F2 with Sesewanua leaf extract creams met the requirements of physical stability, so can be concluded that Sesewanua leaf extract can be formulated into cream preparations with concentration of 0.5% which is physically stable before and after cycling test. Keywords: Sesewanua Leaves, Cream, Physical Stability  ABSTRAK Daun Sesewanua (Clerodendron squamatum Vahl.) berpotensi untuk dibuat sebagai sediaan kosmetik perawatan kulit namun harus dengan formulasi yang tepat agar mencapai efek yang diinginkan. Penelitian ini bertujuan untuk mengetahui stabilitas fisik sediaan krim ekstrak daun sesewanua. Penelitian ini menggunakan metode eksperimental dengan membuat krim ekstrak etanol daun Sesewanua dengan konsentrasi 0,5% dan uji stabilitas fisik. Evaluasi fisik dilakukan sebelum dan sesudah cycling test melalui pengamatan organoleptis, uji homogenitas, uji pH, uji daya sebar, uji daya lekat, uji viskositas, uji sentrifugasi dan penentuan tipe krim. Hasil evaluasi fisik menunjukkan krim F1 tanpa ekstrak daun Sesewanua dan F2 dengan ekstrak daun Sesewanua memenuhi persyaratan stabilitas fisik. Berdasarkan hasil penelitian dapat disimpulkan ekstrak daun sesewanua dapat diformulasi menjadi sediaan krim dengan konsentrasi 0,5% yang stabil secara fisik sebelum dan sesudah cycling test. Kata Kunci : Daun Sesewanua, Krim, Stabilitas Fisik


PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 809
Author(s):  
Gichella C. J. Somba ◽  
Hosea Jaya Edi ◽  
Jainer P. Siampa

ABSTRACT This study aims to test the antibacterial effectiveness of ethanolic extract of Kaliandra leaf cream and evaluate the preparations using parameters of physical properties, physical stability, and sterility. Kaliandra leaf (Calliandra surinamensis) contains compounds such as flavonoid, saponins and tannins that can inhibit the growth of bacteria. This research uses laboratory experimental methods. Cream preparation formula is made with variations in the concentration of ethanol extract of Kaliandra leaves 2%, 3%, 4%, 5%, 6%. Kaliandra leaf extract was obtained by maceration using 96% ethanol solvent. The study of antibacterial test of ethanol extract of Kaliandra leaf extract using the method of Staphylococcus aures produced inhibitory power of 5.09 ± 0.88 mm at a concentration of 6%. The physical evaluation of the cream preparation includes organoleptic examination (odor, color, shape), homogeneity, pH testing, adhesion, dispersion, cycling test. All tests are carried out before and after the cycling test. The results obtained before and after the cycling test showed that the cream preparations met organoleptic requirements, homogeneity, cream pH 6.07 (4.5-6.5), cream adhesion 4.59 seconds (<4 seconds), cream dispersion  6.19 cm (5-7 cm). Cream sterility testing shows that the cream is sterile. It can be concluded that the ethanol extract of Kaliandra leaves cream meets the physical test parameters, is stable and has moderate antibacterial activity. Keywords : Kaliandra Leaf (Calliandra surinamensis), Cream, Antibacterial, Staphylococcous aureus.  ABSTRAK Penelitian ini bertujuan untuk menguji efektivitas antibakteri sediaan krim ekstrak etanol daun Kaliandra dan mengevaluasi sediaan dengan menggunakan parameter uji sifat fisik, stabilitas fisik, dan sterilitas. Daun Kaliandra (Calliandra surinamensis) merupakan tanaman yang mengandung senyawa flavonoid, saponin dan tannin yang mampu menghambat pertumbuhan bakteri. Penelitian ini menggunakan metode eksperimental laboratorium, Formula sediaan krim dibuat dengan variasi konsentrasi ekstrak etanol daun Kaliandra 2%, 3%, 4%, 5%, 6%. Ekstrak daun Kaliandra diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Penelitian uji antibakteri sediaan krim esktrak etanol daun Kaliandra menggunakan metode sumuran pada bakteri Staphylococcus aures menghasilkan daya hambat 5,09±0,88 mm pada konsentrasi 6%. Evaluasi fisik sediaan krim meliputi pemeriksaan organoleptis (bau, warna,  bentuk), homogenitas, pengujian pH, daya  lekat,  daya  sebar, uji cycling test. Semua pengujian dilakukan sebelum dan sesudah cycling test. Hasil penelitian yang didapat sebelum dan sesudah cycling test menunjukkan bahwa sediaan krim memenuhi persyaratan organoleptis, homogenitas, pH krim 6,07 (4,5-6,5), daya lekat krim 4,59 detik (<4 detik), daya sebar krim 6,19 cm (5-7 cm). Pengujian sterilitas krim menunjukan bahwa krim steril. Dapat disimpulkan bahwa sediaan krim ekstrak etanol daun Kaliandra memenuhi parameter uji fisik, stabil dan memiliki aktivitas  antibakteri yang sedang. Kata kunci : Daun Kaliandra (Calliandra surinamensis), Krim, Antibakteri, Staphlococcous aureus.


Author(s):  
Tri Puji Lestari

ABSTRAK Daun Belimbing Wuluh (Averrhoa bilimbi L) memiliki kandungan tanin dan flavonoid. Kandungan Flavonoid dan Tanin yang terdapat dalam belimbing wuluh mampu memberikan efek untuk menyembuhkan kulit yang mengalami kerusakan jaringan sel akibat luka bakar. Ada beberapa cara untuk pemanfaatan Daun Belimbing Wuluh antara lain dengan dibuat dalam sediaan salep. Pada penelitian ini salep dibuat dengan variasi konsentrasi ekstrak daun belimbing wuluh sebesar 10%, 15%, dan 20%  mengguankan basis PEG 4000 dan PEG 400. Ekstrak daun belimbing wuluh diperoleh menggunakan metode maserasi dengan menggunakan etanol 70% sebagai pelarutnya. Salep yang sudah dibuat kemudian dilakukan uji karakteristik meliputi uji organoleptis, homogenitas, daya lekat dan uji daya sebar. Hasil uji organoleptis dan homogenitas di analisa secara deskrisptif kemudian hasil uji daya lekat dan uji daya sebar di analisa dengan menggunakan analisa statistik one-way ANOVA.  Hasil penelitian menunjukan bahwa salep berbentuk semipadat dengan warna hijau muda sampai hijau kehitaman. Hasil uji homogenitas di dapatkan untuk ketiga formula adalah homogen. Nilai daya sebar pada ketiga formula menunjukan perbedaan yang signifikan, pada hasil uji daya lekat dan pH didapatkan tidak ada perbedaan yang signifikan terhadap ketiga formulasi. Dengan demikian perbedaan konsentrasi zat aktif yang digunakan berpengaruh terhadap daya sebar sediaan salep ekstrak daun belimbing wuluh. Kata kunci: Averrhoa bilimbi L; Salep; PEG   ABSTRACT Carambola leaf Wuluh (Averrhoa bilimbi L) contains tannins and flavonoids. The content of flavonoids and tannins contained in starfruit able to give effect to heal skin damaged cell tissue from burns. There were several ways for  utilization  of  Averrhoa  bilimbi  L.  among  others,  made  some  preparations, especially ointment formulation. This study aims to formulate leaf extract ointment preparation starfruit (Averrhoa bilimbi L) with various concentrations of 10%, 15%, and 20% using the PEG 4000 and PEG 400 as a base. Leaf extract obtained from meserasi leaf Averrhoa bilimbi  L  with  70%  ethanol. The ointment that has been made is then subjected to characteristic tests including organoleptic tests, homogeneity, adhesion and spreadability tests. The results of the organoleptic test and homogeneity were analyzed descriptively, then the results of the adhesion test and the spreadability test were analyzed using one-way ANOVA statistical analysis. The results showed that the ointment was semisolid, light green to blackish green. The homogeneity test results obtained for the three formulas are homogeneous. The value of the spreadability of the three formulas showed a significant difference, the results of the adhesion and pH test showed no significant difference between the three formulations. Thus the difference in the concentration of the active substance used affects the spreadability of the starfruit leaf extract ointment. Keywords: Averrhoa bilimbi L; ointment; PEG


2017 ◽  
Vol 53 (2) ◽  
pp. 108 ◽  
Author(s):  
Ratna Sofaria Munir ◽  
Nurmawati Fatimah ◽  
Bambang Hermanto

Indonesia has a variety of herbal plants used as herbal/traditional medicines. Singawalang (Petiveria alliacea) is one of the herbal plants usually used in the United States as a cough medicine because of its expectorant effect. Singawalang plants are also widely used to cure tuberculosis. However, the investigations on the effects of toxicity on this plant leaf extract has not been done. This study aims to investigate the effects of active compounds in singawalang against Mycobacterium tuberculosis using a variety of solvents. The active compound of ethanol extract was obtained by maceration using ethanol solvent. The extract was then fractionated using column chromatography method, and using gradual eluent to produce fraction. The doses used were 0.5 mg/ml; 1 mg/ml; 2 mg/ml; 70% ethanol, h-hexane, benzene, chloroform, ethylacetate, silica gel 60GF254, Middlebrook 7H9, and 7H10. The reactions using Singawalang leaf extract with various solvents resulted in a reduction of the Mycobacterium tuberculosis colony growth, compared with the reaction on control treatment, treatment using DMSO 1%, and treatment using 70% ethanol. As a conclusion, the various solvents used did not make a significant difference. However, control treatment, treatment using DMSO 1%, and treatment using 70% ethanol had significant results.


2021 ◽  
Vol 1 ◽  
pp. 1194-1204
Author(s):  
Khofifah Fitriani ◽  
S Slamet ◽  
Dwi Bagus Pambudi ◽  
Urmatul Waznah

AbstractHair loss is a disorder or disorder of hair apart from the scalp or body skin so that it interferes with various biological functions of hair on the body. Types of hair care cosmetic that is effective in dealing with hair loss are hair tonic. Hair tonic is a preparation for the treatment of the scalp and hair. Bandotan (Ageratum conyzoides L.) is used by the public as medicine and hair care. The purpose of this study was to determine the formulation of hair tonic preparations from the ethanol extract of bandotan leaves according to the requirements and hair growth activity of local male rabbits. The extraction method used the maceration method with 96% ethanol and applied hair tonic extract of bandotan leaves on the skin of local male rabbits (Oryctolagus cuniculus) which were treated as blanks without smearing, positive control (Natur), negative control hair tonic base, concentration bandotan leaf extract. (5%, 10%, 15% and 20%) for 21 days. The data obtained in the form of hair length, and hair weight. Analysis of the data obtained for hair growth activity was seen from the results of the ANOVA test and continued with the BNT test (Least Significance Different). The results of the research for evaluating hair tonic preparations included organoleptic tests, pH tests, viscosity tests, specific gravity tests, and cycling tests. Hair tonic ethanol extract of bandotan leaves has hair growth activity. Hair tonic ethanol extract of bandotan leaves with a concentration of 20% had the highest hair length and hair weight. From the results of one way ANOVA, a significant value was obtained 0.000 < 0.005, so it was concluded that there was a significant difference between treatments, and continued with the BNT test (Least Significance Different) positive control was not significantly different from hair tonic concentration of 20%.Keywords: Bandotan leaves, Hair tonic, Hair growth AbstrakKerontokan rambut adalah suatu gangguan atau kelainan rambut terlepas dari kulit kepala atau kulit tubuh sehingga mengganggu berbagai fungsi biologis rambut terhadap tubuh. Jenis kosmetik perawatan rambut yang efektif mengatasi rambut rontok adalah hair tonic. Hair tonic adalah sediaan untuk perawatan kulit kepala dan rambut. Bandotan (Ageratum conyzoides L.) digunakan oleh masyarakat sebagai obat dan perawatan rambut. Tujuan dari penelitian ini mengetahui formulasi sediaan hair tonic dari ekstrak etanol daun bandotan yang sesuai dengan persyaratan dan aktivitas pertumbuhan rambut pada kelinci jantan lokal. Metode ekstraksi menggunakan metode maserasi dengan etanol 96% dan dilakukan pengolesan hair tonic ekstrak daun bandotan pada kulit kelinci jantan lokal (Oryctolagus cuniculus) yang diberikan perlakuan sebagai blanko tanpa pengolesan, kontrol positif (Natur), kontrol negatif basis hair tonic, ekstrak daun bandotan konsentrasi (5%,10%,15% dan 20%) selama 21 hari. Data yang diperoleh berupa panjang rambut, dan bobot rambut. Analisa data yang diperoleh untuk aktivitas pertumbuhan rambut dilihat dari hasil uji ANOVA dan dilanjutkan dengan uji BNT (Beda Nyata Terkecil). Hasil penelitian untuk evaluasi sediaan hair tonic antara lain uji organoleptis, uji pH, uji viskositas, uji bobot jenis, dan uji cycling test. Hair tonic ekstrak etanol daun bandotan memiliki aktivitas pertumbuhan rambut. Hair tonic ekstrak etanol daun bandotan dengan konsentrasi 20% memiliki panjang rambut dan bobot rambut tertinggi. Dari hasil one way ANOVA didapatkan nilai signifikan 0,000 < 0,005 sehingga disimpulkan adanya perbedaan signifikan antar perlakuan, dan dilanjutkan uji BNT (Beda Nyata Terkecil) didapatkan kontrol positif tidak berbeda nyata dengan hair tonic konsentrasi 20%.Kata kunci : Daun bandotan; Hair tonic; Pertumbuhan rambut


2020 ◽  
Vol 1 (1) ◽  
pp. 73
Author(s):  
Fajriansyah Fajriansyah

Background: The effort to control mosquitoes can be done by terminating the life cycle of mosquitoes, eradication of the larval sadium, namely larvicides. Rambutan leaves (Nephelium lappaceum L.) contain tannin compounds, saponins. Saponins are destroying red blood cells through the reaction of hemolysin, are toxic to cold-blooded animals, including cold-blooded insects, one of the insects that often interfere with human life is mosquitoes.Objectives: The purpose of this study was to determine the extract of rambutan leaves on the death of Aedes aegypti larvae Methods: This research is an experimental research. The object of research was Aedes aegypti mosquito larvae. The study was conducted at the writer home of Lamjamee Village, Banda Aceh. Research time is August 23-25, 2017. Type of research is an experimental study that is the effect of rambutan leaf extract  on the death of Aedes aegypti larvae. The object of research is the population of Aedes aegypti larvae. The research subjects taken were Aedes aegypti mosquito larvae that lived because of the administration of rambutan leaf extract with a concentration of 5%, 10%, 15%. Statistical data analysis used Anova LSD test.Results: The results showed that the death of Aedes aegypti larvae by using rambutan leaf extract (Nephelium lappaceum L.) dose of 5% with an average of 19.7. Dose 10% had an average of 34.3, and a dose of 15% of the average mortality was 43.7. There is a significant difference between each dose of the mortality rate of Aedes aegypti larvae, with a value of F= 28.1 (F-count > F-table = 4.07).Conclusion: Various concentrations of the three treatments of rambutan leaf extract namely 5%, 10% and 15% are very effective against the death of Aedes aegypti larvae. Suggestions, can be used and practiced in everyday life, so as to reduce the use of chemical insecticides.


2021 ◽  
Vol 4 (2) ◽  
pp. 98-103
Author(s):  
Nurista Dida Ayuningtyas ◽  
Agustina Putri Pitarisa Sudarsono ◽  
Anastasiya Sisca Yuswanti

Background : Lime leaves contain flavonoids, tannins, and saponins. This content is known to have activity as an antibacterial and protective of gums and teeth. Based on these activities, lime leaves can be developed as an active ingredient in gel toothpaste preparations. In the manufacture of gel toothpaste, the component that plays an important role is the gelling agent. Purpose: In this study, a variation of the gelling agent Carbomer 940 will be used to make a gel toothpaste with lime leaf extract. Methods: The independent variable used is Carbomer 940 with a variation of 3 formulas, namely 0.6%; 1.2%; and 1.8%. The evaluations carried out on gel toothpaste were organoleptic, homogeneity, pH, adhesion, spreadability and pH. Data analysis was carried out with SPSS software to see the differences between each formula. Results: The results showed that the formula 1-3 was homogeneous with a clear, homogeneous color, and a characteristic smell of menthol with a pH range of 5.85-7.49; spreading area 20.60-29.91 cm2, adhesion 6.01-35.1 seconds. The results of statistical tests with one-way ANOVA obtained a significant difference between each formula.  


2020 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Dita Puspita Sari ◽  
Didit Aspriyanto ◽  
Irham Taufiqurrahman

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.


2021 ◽  
Vol 2 (2) ◽  
pp. 142
Author(s):  
Syahrizal Syahrizal ◽  
Junaidi Junaidi

Background: Flies (Musca domestica) can act as vectors of typhus, dysentery, and cholera. Various methods have been used to control flies from around human life, either mechanically (light traps), a chemical in the form of toxic baits or insecticides, or with scents that are repellent. A mixture of curry leaf extract with soursop leaves can be used as an insecticide to repel house flies. Because curry leaves and soursop leaves contain compounds, tannins, saponins, and flavonoids that flies do not like.Objective: The purpose of this study was to determine the effectiveness of a mixture of curry leaves with soursop leaves as a natural insecticide in repelling the flies (Musca Domestica).Methods: This research is an experimental design with a mixture of curry leaf extract with soursop leaves on the power of repelling house flies. This research was conducted in 2019 in Darul Kamal District, Aceh Besar. The object of the research was 450 house flies, which consisted of five treatments with three repetitions. Data analysis was carried out with the one-way ANOVA statistical test.Results: The descriptive results showed that at a dose of 10gr/100ml the average flies were able to repel 37 flies, at a dose of 20 gr/100ml were 44, at a dose of 30 gr/100ml were 59 and at a dose of 40 gr/100ml was 70. Of the four treatments, the most effective dose was at a dose of 40gr/100ml, which was 70 (77.7%). Statistically, it showed that there was a significant difference (p < 0.05) between various doses/treatments of mixed extracts of curry leaves (Murraya koeginii) and soursop leaves (Annona muricata Linn) on the repelling power of house flies (Musca domestica), with p-value = 0.000.Conclusion: A mixture of curry leaf extract (Murraya koeginii) with soursop leaf (Annona muricata Linn) is effective as an insecticide in repelling house flies (Musca domestica).


2019 ◽  
Vol 16 (02) ◽  
pp. 40
Author(s):  
Malinda Prihantini ◽  
Elya Zulfa ◽  
Listyana Dewi Prastiwi ◽  
Ikha Dyah Yulianti

ABSTRACT The nanoparticle system encapsulates and protects flavonoids of suji leaves from photolysis and oxidation instability. Chitosan is a biocompatible, biodegradable and non-toxic nanoparticles forming polymer. Ultrasonication is a materials mixing technique under high vibration energy. The ultrasonication time affects the particle size. The aim of this study was to determine the effect of the ultrasonication time on physical characteristics of chitosan nanoparticles of suji (Pleomele angustifolia) leaf ethanol extract and its physical stability after cycling test. Ethanol extract of suji leaves (EEDS) was obtained by maceration using 70% ethanol. Chitosan EEDS nanoparticles were made under ionic gelation method using an ultrasonication time of 3 minutes (FI), 6 minutes (FII), and 9 minutes (FIII). The physical characterization of nanoparticles includes particle size, polydispersity index, and zeta potential. Chitosan EEDS nanoparticles with the best physical characteristics (FIII) were tested for stability using cycling test method. The data obtained were analyzed using the Anova statistical method with a 95% confidence level. The size of EEDS chitosan nanoparticles (nm) was significantly different in all formulas, FI (374.47), FII (288.43), and FIII (233.37). The polydispersity index of FI (0.38) and FIII (0.65) were significantly different, while FII (0.41) was not significantly different. The zeta potential (mV) FI (51.70), FII (46.10), and FIII (48.60) were not significantly different in all formulas. The physical characteristics of Formula III after Cycling Test showed a particle size of 455.0 nm, a polydispersity index of 0.174, and a zeta potential of 20.1 mV. Keywords: ultrasonication, nanoparticles, chitosan, cycling test, suji leaf (Pleomele angustifolia)


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