Effects of water acidification on immune responses of the gercacinid, Cardiosoma armatum (Herklots, 1851)

2021 ◽  
pp. 4006-4013
Author(s):  
Lawal-Are A.O. ◽  
Moruf R.O. ◽  
Olaniyi I.O. ◽  
Okafor D.S.
Keyword(s):  
Energy Use ◽  
Hematological Parameters ◽  
Biological Response ◽  
Control Group ◽  
Water Acidification ◽  
Sod Activity ◽  
Immune Parameters ◽  
Chemical Pollutants ◽  
Acid Base Status ◽  
Ph Variations

The biological response to chemical pollutants reflects the acid–base status of an aquatic ecosystem. The gercacinid, Cardiosoma armatum (75±0.1 g) was exposed to acidified waters to evaluate the effects on its immune parameters. The crabs were exposed to pH 4.0, 5.0, 6.0 and 7.8 (control) for 28 days. The hematological parameters of control crabs and crabs exposed to varied doses of acidified water indicated a marked reduction. Significant (p<0.05) higher alkaline phosphatase and albumen were obtained in pH 4.0, 5.0 and 6.0 compared to control; other values were mostly similar to control. The highest superoxide dismutase (SOD) (252.61±0.06 min/mg pro) was recorded in control group, while highest CAT activity (2.08±0.16 min/mg protein) was recorded in crabs exposed to pH 4 treatment. Furthermore, the control group's SOD activity was significantly higher than the exposed groups. With a lower pH, the quantities of malondialdehyde increased substantially and were significantly different from the control group. While these findings demonstrate that changes in pH have limited impact on energy use, decreasing immune system conditions show that C. armatum is susceptible to pH variations and may be influenced in aquaculture, where a pH drop is more prominent.

scholarly journals Subchronic Toxicity of Green Algae (Spyrogyra sp.) Ethanolic Extract on Hematologic Parameters

2016 ◽  
Vol 5 (4) ◽  
pp. 484
Author(s):  
Nina Salamah ◽  
Wahyu Widyaningsih ◽  
Hari Susanti ◽  
Anggita Devi ◽  
Anita Wening Sejati ◽  
...  
Keyword(s):  
Green Algae ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Test Group ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Immune Parameters ◽  
Hematology Parameters

<p>Green Algae, an organism with active substance such as phytomelatonin, has potential to be developed as Indonesian traditional medicine. As the long term addition of Green Algae ethanol extract (<em>Ekstrak etanol ganggang hijau</em>, EEGH) influences the hematology system, in this paper, the safety test was done to ensure the safety of its use through subchronic toxicity test of EEGH on the hematology parameters of Wistar rats. The test group consisted of three groups treated with EEGH 100 mg/kg, 200 mg/kg, and 400 mg/kg, while the control group was given by 0.5% CMC-Na, with 8 rats each respectively. By using blood samples taken from orbital sinus on the 29<sup>th</sup> day, common hematologic parameters (erythrocytes, leukocytes, and hemoglobin level), the parameters of hemostasis (platelets, pT, aPTT, BT) and immune parameters (Differential Leukocytes Counts include neutrophils segment, lymphocytes, monocytes, and eosinophils) were finally observed and showed that the 28 days-addition of EEGH increase the hematological parameters of Wistar rats.</p>

scholarly journals Evaluation of Oxidative Stress and some Biochemical Parameters in Normal Pregnant Women

2020 ◽  
Vol 8 (1) ◽  
pp. 17-23
Author(s):  
Musher I. Kakey ◽  
Kamaran K. Abdoulrahman ◽  
Gulbahar R. Uthman
Keyword(s):  
Pregnant Women ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
First Trimester ◽  
Control Group ◽  
Sod Activity ◽  
Nonsignificant Difference ◽  
Group 2

Pregnancy is associated with many metabolic changes in normal pregnant woman, this leads to change in physiological, biochemical, and hematological parameters drastically. The test subjects were selected among those attending to Maternity Teaching Hospital in Erbil Governorate between March 2017 and August 2017. Four groups of individuals were included in this study, 230 pregnant women divided into three groups (Group 1 first trimester, Group 2 second trimester, and Group 3 third trimester) and Group 4 contained 90 nonpregnant women as control. Full automatic chemical analyzer (Cobas C311, Germany) was used to determine the biochemical parameters. The EL ×800 Absorbance Microplate Reader from BioTek (USA) instruments is used to measure the activity of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in serum. The results of this study showed a significant increase in serum albumin, glucose, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, urea, cholesterol, low-density lipoprotein, and MDA in pregnant women as compared to the control group, also show significant decrease in high-density lipoprotein (HDL) level, SOD activity, and CAT activity in pregnant women as compared to nonpregnant women, and nonsignificant difference in uric acid, and HDL.

scholarly journals USE OF A DIETARY SUPPLEMENT BASED ON PROPOLIS IN FEED

2020 ◽  
Vol 2020 (3) ◽  
pp. 52-64
Author(s):  
Nikolay Gaponov
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Energy Use ◽  
Hematological Parameters ◽  
Average Daily Gain ◽  
Metabolic Energy ◽  
Control Group ◽  
Large White ◽  
Fattening Period ◽  
Fattening Pigs

For the first time, the effect of propolis drug, when introduced into the diets of rearing pigs and fattening pigs, on metabolism, energy, hematological parameters was studied. To this end, two scientific and economic experiments were carried out with piglets rearing a large white breed and with pigs of the same breed during the fattening period, as well as two physiological experiments to determine the digestibility of the main nutrients of feed by animals and to determine the balance of energy, nitrogen, calcium, phosphorus, one of which was carried out at the end of the rearing period (at 4 months of age) and the other during the fattening period (at 6 months of age). During the experiments, it was found that the use of propolis water-alcohol emulsion in the diet helps to increase the average daily gain in rearing pigs by 30.13% and in young fattening pigs by 4.46%. The digestibility of crude protein in rearing pigs increases by 8.50%, nitrogen-free extractives by 1.8%, organic matter by 3.27%, fiber by 8.19%. In this case, the efficiency of using exchange energy increases by 5.45% and the use of nitrogen, calcium and phosphorus by 30.70%, 17.10% and 11.86%, respectively. And during the fattening period of young pigs, it contributes to a better use of nitrogen in comparison with the control group by 11.5%, calcium by 4.75% and phosphorus by 3.9%; increases the efficiency of metabolic energy use by 5.49%, improves the digestibility of crude protein by 1.61%, organic matter by 1.42%, crude fat by 5.16%, fiber by 6.3% and nitrogen-free extractives by 0.82%.

scholarly journals Subchronic Toxicity of Green Algae (Spyrogyra sp.) Ethanolic Extract on Hematologic Parameters

2016 ◽  
Vol 5 (4) ◽  
pp. 484
Author(s):  
Nina Salamah ◽  
Wahyu Widyaningsih ◽  
Hari Susanti ◽  
Anggita Devi ◽  
Anita Wening Sejati ◽  
...  
Keyword(s):  
Green Algae ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Test Group ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Immune Parameters ◽  
Hematology Parameters

<p>Green Algae, an organism with active substance such as phytomelatonin, has potential to be developed as Indonesian traditional medicine. As the long term addition of Green Algae ethanol extract (<em>Ekstrak etanol ganggang hijau</em>, EEGH) influences the hematology system, in this paper, the safety test was done to ensure the safety of its use through subchronic toxicity test of EEGH on the hematology parameters of Wistar rats. The test group consisted of three groups treated with EEGH 100 mg/kg, 200 mg/kg, and 400 mg/kg, while the control group was given by 0.5% CMC-Na, with 8 rats each respectively. By using blood samples taken from orbital sinus on the 29<sup>th</sup> day, common hematologic parameters (erythrocytes, leukocytes, and hemoglobin level), the parameters of hemostasis (platelets, pT, aPTT, BT) and immune parameters (Differential Leukocytes Counts include neutrophils segment, lymphocytes, monocytes, and eosinophils) were finally observed and showed that the 28 days-addition of EEGH increase the hematological parameters of Wistar rats.</p>

Erythrocyte and reticulocyte parameters during biological monitoring of lead exposure to the body

2020 ◽  
Vol 60 (11) ◽  
pp. 873-876
Author(s):  
Anastasiya G. Khotuleva ◽  
Mariya S. Kozyreva
Keyword(s):  
Biological Monitoring ◽  
Hematological Parameters ◽  
Lead Level ◽  
Statistical Processing ◽  
Absorption Spectrometry ◽  
The Body ◽  
Control Group ◽  
Processing Plant ◽  
Hematological Disorders ◽  
Globin Synthesis

Introduction. The most susceptible to lead is the hematopoietic system of hematopoietic organs due to lead inhibition of heme and globin synthesis and cytotoxic effect on the membrane of Mature red blood cells. The aim of study was to evaluate the informative value of the study of erythrocyte and reticulocyte parameters determined on modern hematological analyzers in patients working in contact with lead during medical and biological monitoring. Materials and methods. 45 employees of the lead battery processing plant and 30 persons of control group were examined. The level of lead in the blood was determined by atomic absorption spectrometry, δ-ALA in the urine-by the reaction of pyrol formation with acetylacetone in terms of gram of creatinine, the study of hematological parameters was performed on a Sysmex HT-2000i analyzer. Statistical processing of the results was performed using the program STATISTICA 10.0. Results. Significant changes in erythrocytic (RDW) and reticulocytic (RET, IRF, LFR, MFR, HFR, RET-He) parameters, erythropoietin in workers in contact with lead compared to the control group, changes in MCV, MCH, RDW, RET indicators in the group working in dynamics after 2 years were revealed. Associations of hematological parameters with biomarkers of exposure and effect (lead level in blood and ALA in urine) were revealed. Conclusions. Assessment of erythrocyte (MCV, MCH, RDW) and reticulocyte parameters (RET% and their distribution by maturity) in dynamics during periodic medical examinations of workers in contact with lead allows us to detect the development of hematological disorders at early stages.

Probiotics in the diets of laying quails

2020 ◽  
pp. 60-66
Author(s):  
O. Merzlyakova ◽  
V. Rogachyev ◽  
V. Chegodaev
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Egg Production ◽  
Positive Impact ◽  
Hematological Parameters ◽  
Economic Effect ◽  
Egg Laying ◽  
Control Group ◽  
Egg Mass ◽  
The Cost

The efficiency of introducing probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed into the diets of laying quails has been studied. The experiment lasting 182 days has been carried out on four groups of quails with 30 heads in each. The quails have been housed in the broiler battery in compliance with the required microclimate conditions. Quails of all groups have been received the main diet (compound feed) developed taking into account their age and physiological characteristics. The quails of the 1st, 2nd and 3rd experimental groups in addition to the main diet received probiotics (150 g/t compound feed) based on strains Bacillus subtilis, Bacillus licheniformis and their consortium, respectively. It has been found that feeding the laying quails of the consortium of strains Bacillus subtilis and Bacillus licheniformis had the most significant positive impact on their productive performance, it allowed to increase egg production by 7,81 %, egg laying intensity by 5,0 %, egg mass yield by 9,77 %, while reducing feed expenditures for 10 eggs by 13,35 %. The yield of hatching eggs has been increased by 7,03 %, hatchability of chickens from laid and fertilized eggs by 8,33 and 8,35 %, brooding waste decreased by 21,74 %. Hematological parameters of quails during the whole experiment were within the physiological norm. The economic effect calculated on the basis of data on the cost of compound feed, probiotics and the cost of sold eggs of quail laying was 14,56 % in the 3rd experimental group (in relation to the control group).

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

The Predictive Role of Biochemical Plasma Factors in Patients with Severe Traumatic Brain Injuries

2019 ◽  
Vol 70 (5) ◽  
pp. 1754-1757
Author(s):  
Marius Toma Papacocea ◽  
Ioana Anca Badarau ◽  
Mugurel Radoi ◽  
Ioana Raluca Papacocea
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Blood Cell ◽  
Brain Injuries ◽  
Hematological Parameters ◽  
Group Versus ◽  
Public Health Problem ◽  
Traumatic Brain Injuries ◽  
High Rate ◽  
Control Group

Traumatic brain injuries (TBI) represent a high impact public health problem due to a high rate of death , long term disability and occurrence especially in young adults. Despite several promising animal studies, several parameters were proposed as biological markers and were assessed for this aim. Our study proposes the study of the early biochemical changes in association to hematological parameters for severe TBI patients prognosis. 43 patients with acute TBI were included in study based on clinical, laboratory and imagistic findings. The severity of the TBI was established by Glasgow Coma Scale GCS 3-8. In all patients were evaluated hematologic parameters (Red blood cell count - RBC, Hematocrit, blood Hemoglobin, White blood cell - WBC, Platelet count and biochemical parameters (glucose, urea, creatinine, electrolytes). Outcome was expressed as Glasgow Outcome Scale (GOS), between 1-5. Values were compared to control group -15 cases. Significant early differences in body temperature, heart rate, and systolic blood pressure were observed in TBI group versus control (p[0.05). After correlation, laboratory findings significantly associated to severe outcome - GOS = 1, 2 - (p[0.05) were plasma Na decrease and significant glucose increase. An early increase of temperature and decrease of Na may predict a severe outcome in patients with acute TBI; association with shifts in heart rate and blood pressure, imposes aggressive treatment measures.

scholarly journals The Dwarfing Effects of Different Plant Growth Retardants on Magnolia wufengensis L.Y. Ma et L. R. Wang

Forests ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Xiaodeng Shi ◽  
Siyu Chen ◽  
Zhongkui Jia
Keyword(s):  
Plant Growth ◽  
Soluble Sugar ◽  
Control Group ◽  
Growth Retardants ◽  
Sod Activity ◽  
Node Number ◽  
Plant Growth Retardants ◽  
One Year ◽  
Prohexadione Calcium ◽  
Magnolia Wufengensis

The effects of varieties, concentrations, and number of applications of plant growth retardants (PGRs) on the morphological, physiological, and endogenous hormones of Magnolia wufengensis L.Y. Ma et L. R. Wang were assessed to obtain the most suitable dwarfing protocol for M. wufengensis and to provide theoretical support and technical guidance for the cultivation and promotion of this species. One-year-old M. wufengensis ‘Jiaohong No. 2’ grafted seedlings served as the experimental materials. In the first part of the experiment, three PGRs (uniconazole, paclobutrazol, prohexadione calcium), three concentrations (500, 1000, 1500 ppm), and three applications (one, three, and five applications) were applied in dwarfing experiments to perform L9 (34) orthogonal tests. In the second part of the study, dwarfing experiments were supplemented with different high uniconazole concentrations (0, 1500, 2000, 2500 ppm). Spraying 1500 ppm uniconazole five times achieved the best M. wufengensis dwarfing effect, related indicators of M. wufengensis under this treatment were better than other treatment combinations. Here, M. wufengensis plant height, internode length, scion diameter, and node number were significantly reduced by 56.9%, 62.6%, 72.8%, and 74.4%, respectively, compared with the control group. This treatment increased superoxide dismutase (SOD) activity by 66.0%, peroxidase (POD) activity by 85.0%, soluble protein contents by 43.3%, and soluble sugar contents by 27.6%, and reduced malondialdehyde (MDA) contents by 32.1% in leaves of M. wufengensis compared with the control. The stress resistance of M. wufengensis was enhanced. The treatment also reduced gibberellin (GA3) levels by 73.0%, auxin (IAA) by 58.0%, and zeatin (ZT) by 70.6%, and increased (abscisic acid) ABA by 98.1% in the leaves of M. wufengensis. The uniconazole supplementation experiment also showed that 1500 ppm was the optimal uniconazole concentration. The leaves exhibited abnormalities such as crinkling or adhesion when 2000 or 2500 ppm was applied. Given the importance of morphological indicators and dwarfing for the ornamental value of M. wufengensis, the optimal dwarfing treatment for M. wufengensis was spraying 1500 ppm uniconazole five times.

scholarly journals Effect of Acetic Acid and Sodium Bicarbonate Supplemented to Drinking Water on Water Quality, Growth Performance, Organ Weights, Cecal Traits and Hematological Parameters of Young Broilers

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1865
Author(s):  
Yordan Martínez ◽  
Cristopher Isaac Almendares ◽  
Cristhian José Hernández ◽  
Mavir Carolina Avellaneda ◽  
Ana Melissa Urquía ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Acetic Acid ◽  
Sodium Bicarbonate ◽  
Growth Performance ◽  
Hematological Parameters ◽  
Relative Weight ◽  
Biological Response ◽  
Blood Parameters ◽  
Organ Weights

To evaluate the effect of acetic acid and sodium bicarbonate supplemented to drinking water on water quality, growth performance, relative organ weights, cecal traits and hematological parameters of broilers, a total of 456 one-day-old Cobb MV × Cobb 500 FF mixed broilers were randomly placed in three experimental treatments, with four replicates per treatment and 38 birds per replicate, for 10 days. The treatments consisted of the use of acetic acid (0.4%; T1) as acidifier, an apparently neutral pH (T2) and sodium bicarbonate (1%; T3) as alkalizer of the drinking water. T3 showed the highest values (p < 0.05) for total dissolved solids, electrical conductivity, salinity and pH. T1 and T2 showed the same productive response (p > 0.05); however, T3 decreased (p < 0.05) body weight, feed intake and the relative weight of the pancreas and immune organs and increased (p < 0.05) water intake, mortality and relative weight of the heart and liver. Likewise, T3 increased (p < 0.05) the cecal pH, although without changes for the cecal lactic cecal bacteria count and blood parameters (p > 0.05). The acid pH of the drinking water had no effect on the biological response of broilers compared to T2; however, the T3 provoked high mortality, ascites, low productivity and abnormal growth of some organs.

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