scholarly journals Novel Alhagi maurorum leaves mediated synthesis of titanium nanoparticles for human breast carcinoma applications: a preclinical trial

2021 ◽  
Author(s):  
Jiahui Ye ◽  
Jiajun Shi ◽  
Meng Zhang ◽  
Yin Zhang ◽  
jinqiu Tao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
T Cell ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Preclinical Trial ◽  
X Ray ◽  
Titanium Nanoparticles

IntroductionIn this study, titanium nanoparticles (TiNPs) were synthesized in an aqueous medium using Alhagi maurorum extract as stabilizing and reducing agents.Material and methodsUltraviolet–visible spectrophotometry (UV-Vis), fourier-transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), scanning electron microscopy (SEM), and Energy Dispersive X-ray Spectrometry (EDS) were the techniques to characterize the biosynthetic of TiNPs. According to the XRD analysis. To survey the anti-human breast cancer effects of TiNPs, MTT assay was used on the common breast cancer cell lines i.e., breast cancer (Breast adenocarcinoma (MCF7), infiltrating ductal cell carcinoma (Hs 319.T), inflammatory carcinoma of the breast (UACC-732), and metastatic carcinoma (MDA-MB-453) cell lines.Results16.08 nm was measured for TiNPs crystal size. SEM images exhibited a uniform spherical morphology in range size of 12.16 to 43.46 nm for the biosynthesized nanoparticles respectively. The cell viability of breast carcinoma cells decreased dose-dependently in the titanium nanoparticles presence. The IC50 of A. maurorum and titanium particles on MCF7 cell line were 680 and 359 µg/mL, on Hs 319.T cell line were 507 and 191 µg/mL, on UACC-732 cell line were 477 and 217 µg/mL, and on MDA-MB-453cell line were 507 and 191 µg/mL, respectively. TiNPs had high anti-breast cancer activities dose-dependently against MCF7, Hs 319.T, UACC-732, and MDA-MB-453 cell lines.ConclusionsThe best result of anti-breast cancer effects was seen in the case of the Hs 319.T cell line.

Novel Alhagi maurorum leaves mediated synthesis of titanium nanoparticles for human breast carcinoma applications: a preclinical trial

2021 ◽  
Author(s):  
Jiahui Ye ◽  
Jiajun Shi ◽  
Meng Zhang ◽  
Yin Zhang ◽  
jinqiu Tao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
T Cell ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Preclinical Trial ◽  
X Ray ◽  
Titanium Nanoparticles

IntroductionIn this study, titanium nanoparticles (TiNPs) were synthesized in an aqueous medium using Alhagi maurorum extract as stabilizing and reducing agents.Material and methodsUltraviolet–visible spectrophotometry (UV-Vis), fourier-transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), scanning electron microscopy (SEM), and Energy Dispersive X-ray Spectrometry (EDS) were the techniques to characterize the biosynthetic of TiNPs. According to the XRD analysis. To survey the anti-human breast cancer effects of TiNPs, MTT assay was used on the common breast cancer cell lines i.e., breast cancer (Breast adenocarcinoma (MCF7), infiltrating ductal cell carcinoma (Hs 319.T), inflammatory carcinoma of the breast (UACC-732), and metastatic carcinoma (MDA-MB-453) cell lines.Results16.08 nm was measured for TiNPs crystal size. SEM images exhibited a uniform spherical morphology in range size of 12.16 to 43.46 nm for the biosynthesized nanoparticles respectively. The cell viability of breast carcinoma cells decreased dose-dependently in the titanium nanoparticles presence. The IC50 of A. maurorum and titanium particles on MCF7 cell line were 680 and 359 µg/mL, on Hs 319.T cell line were 507 and 191 µg/mL, on UACC-732 cell line were 477 and 217 µg/mL, and on MDA-MB-453cell line were 507 and 191 µg/mL, respectively. TiNPs had high anti-breast cancer activities dose-dependently against MCF7, Hs 319.T, UACC-732, and MDA-MB-453 cell lines.ConclusionsThe best result of anti-breast cancer effects was seen in the case of the Hs 319.T cell line.

The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

2018 ◽  
Vol 16 (2) ◽  
pp. 127-137
Author(s):  
Paula Sofia Coutinho Medeiros ◽  
Ana Lúcia Marques Batista de Carvalho ◽  
Cristina Ruano ◽  
Juan Carlos Otero ◽  
Maria Paula Matos Marques
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Breast Adenocarcinoma ◽  
Coumaric Acid ◽  
Human Breast ◽  
The Impact

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells. </P><P> Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line. Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability. Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

scholarly journals Histone Deacetylase Inhibitors and Microtubule Inhibitors Induce Apoptosis in Feline Luminal Mammary Carcinoma Cells

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 502
Author(s):  
Filipe Almeida ◽  
Andreia Gameiro ◽  
Jorge Correia ◽  
Fernando Ferreira
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Mammary Carcinoma ◽  
Human Breast Cancer ◽  
Histone Deacetylase Inhibitors ◽  
Trichostatin A ◽  
Human Breast ◽  
Antitumor Effects

Feline mammary carcinoma (FMC) is the third most common type of neoplasia in cats, sharing similar epidemiological features with human breast cancer. In humans, histone deacetylases (HDACs) play an important role in the regulation of gene expression, with HDAC inhibitors (HDACis) disrupting gene expression and leading to cell death. In parallel, microtubules inhibitors (MTIs) interfere with the polymerization of microtubules, leading to cell cycle arrest and apoptosis. Although HDACis and MTIs are used in human cancer patients, in cats, data is scarce. In this study, we evaluated the antitumor properties of six HDACis (CI-994, panobinostat, SAHA, SBHA, scriptaid, and trichostatin A) and four MTIs (colchicine, nocodazole, paclitaxel, and vinblastine) using three FMC cell lines (CAT-MT, FMCp, and FMCm), and compared with the human breast cancer cell line (SK-BR-3). HDACis and MTIs exhibited dose-dependent antitumor effects in FMC cell lines, and for all inhibitors, the IC50 values were determined, with one feline cell line showing reduced susceptibility (FMCm). Immunoblot analysis confirmed an increase in the acetylation status of core histone protein HDAC3 and flow cytometry showed that HDACis and MTIs lead to cellular apoptosis. Overall, our study uncovers HDACis and MTIs as promising anti-cancer agents to treat FMCs.

Determination of Vulpinic Acid Effect on Apoptosis and mRNA Expression Levels in Breast Cancer Cell Lines

2019 ◽  
Vol 18 (14) ◽  
pp. 2032-2041 ◽  
Author(s):  
Nil Kılıç ◽  
Sümer Aras ◽  
Demet Cansaran-Duman
Keyword(s):  
Breast Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cells ◽  
Breast Cancer Cell Lines ◽  
Human Breast

Objective: Breast cancer is one of the most common diseases among women worldwide and it is characterized by a high ratio of malignancy and metastasis and low rate of survival of patients. Due to limited treatment options, the discovery of alternative therapeutic agents and clarifying the molecular mechanism of breast cancer development may offer new hope for its treatment. Lichen secondary metabolites may be one of these therapeutic agents. Methods: In this study, the effects of Vulpinic Acid (VA) lichen secondary metabolite on the cell viability and apoptosis of breast cancer cells and non-cancerous cell line were investigated. Quantitative polymerase chain reaction was also performed to determine changes in the expression of apoptosis-related genes at a molecular level. Results: The results demonstrated that VA significantly inhibited the cell viability and induced apoptosis of human breast cancer cells. The highest rates of decreased growth were determined using the IC50 value of VA for 48h on MCF-7 breast cancer cell. Interestingly, VA treatment significantly reduced cell viability in all examined breast cancer cell lines compared to their non-cancerous human breast epithelial cell line. This is the first study on the investigation of the effects of VA on the molecular mechanisms associated with the expression of apoptosis-related genes in breast cancer cell lines. Results demonstrated that the gene expression of P53 genes was altered up to fourteen-fold levels in SK-BR-3 cell lines whereas it reached 2.5-fold in the MCF-12A cell line after treatment with VA. These observations support that VA induces apoptosis on the breast cancer cells compared with the non-cancerous human breast epithelial cell line. Conclusion: It is implicated that VA may be a promising novel molecule for the induction of apoptosis on breast cancer cells.

Evaluation of Cytotoxicity of normal Vero and Anticancer Activity of Human Breast Cancer Cell Lines by Aqueous Unripe Fruit Extract of Solanum torvum

2021 ◽  
pp. 3504-3508
Author(s):  
D. Shanthi ◽  
R. Saravanan
Keyword(s):  
Anticancer Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Breast Adenocarcinoma ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Solanum Torvum ◽  
Adenocarcinoma Cell ◽  
Unripe Fruits

In the present study aqueous extract of Solanum torvum unripe fruits was used to evaluate its cytotoxic effect and anticancer activity through in vitro studies by 3-(4, 5 dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide- MTT assay) on Normal VERO cell line and MCF-7 (Human breast adenocarcinoma cell line). Aqueous extracts of Solanum torvum unripe fruits was found to be effective in the prevention of cell proliferation by breast adenocarcinoma cell lines at 1000 µg/ml from the results obtained during 24 hours of incubation.

A novel animal model of human breast cancer metastasis to the spine: a pilot study using intracardiac injection and luciferase-expressing cells

2013 ◽  
Vol 18 (3) ◽  
pp. 217-225 ◽  
Author(s):  
Patricia Zadnik ◽  
Rachel Sarabia-Estrada ◽  
Mari L. Groves ◽  
Camilo Molina ◽  
Christopher Jackson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Real Time ◽  
Human Breast Cancer ◽  
Cancer Metastasis ◽  
Spinal Metastases ◽  
Disease Process ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Intracardiac Injection

Object Metastatic spine disease is prevalent in cancer victims; 10%–30% of the 1.2 million new patients diagnosed with cancer in the US exhibit spinal metastases. Unfortunately, treatments are limited for these patients, as disseminated disease is often refractory to chemotherapy and is difficult to treat with surgical intervention alone. New animal models that accurately recapitulate the human disease process are needed to study the behavior of metastases in real time. Methods In this study the authors report on a cell line that reliably generates bony metastases following intracardiac injection and can be tracked in real time using optical bioluminescence imaging. This line, RBC3, was derived from a metastatic breast adenocarcinoma lesion arising in the osseous spine of a rat following intracardiac injection of MDA-231 human breast cancer cells. Results Upon culture and reinjection of RBC3, a statistically significantly increased systemic burden of metastatic tumor was noted. The resultant spine lesions were osteolytic, as demonstrated by small animal CT scanning. Conclusions This cell line generates spinal metastases that can be tracked in real time and may serve as a useful tool in the study of metastatic disease in the spine.

scholarly journals The Characterization of Cell Line CRL-2335 as a Basal-Like Breast Carcinoma Model

2011 ◽  
Vol 5 ◽  
pp. BCBCR.S7087
Author(s):  
Lori A. Hill Neves ◽  
Lishann Ingram ◽  
Melissa B. Davis
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Ethnic Disparities ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Biochemical Pathways ◽  
Key Factor ◽  
Basal Like Breast Cancer

Basal-like breast cancer has been reported to be the most aggressive and deadly carcinoma sub-type. Patients diagnosed with this subtype have a less than 50% five-year survival. In addition, many studies have reported that this sub-type is more prevalent in specific ethnic groups and is believed to be a key factor that drives certain ethnic disparities in mortality. In order to effectively study this sub-type and determine unique gene expression and biochemical pathways which sustain this cancer's growth, we sought to identify human breast cancer cell lines that represent a model for the basal-like subtype. Here, we report our findings which indicate the African American cell line CRL-2335 is a true representative of basal-like breast carcinoma.

scholarly journals Genotoxic Effects of Tributyltin and Triphenyltin Isothiocyanates, Cognate RXR Ligands: Comparison in Human Breast Carcinoma MCF 7 and MDA-MB-231 Cells

2019 ◽  
Vol 20 (5) ◽  
pp. 1198 ◽  
Author(s):  
Luba Hunakova ◽  
Eva Horvathova ◽  
Karolina Majerova ◽  
Pavel Bobal ◽  
Jan Otevrel ◽  
...  
Keyword(s):  
Dna Damage ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Human Breast Carcinoma ◽  
Human Breast ◽  
Receptor Ligands ◽  
Estrogen Receptor Positive ◽  
Breast Carcinoma Cell Lines ◽  
Mcf 7

The cytotoxicity of two recently synthesized triorganotin isothiocyanate derivatives, nuclear retinoid X receptor ligands, was tested and compared in estrogen-receptor-positive MCF 7 and -negative MDA-MB-231 human breast carcinoma cell lines. A 48 h MTT assay indicated that tributyltin isothiocyanate (TBT-ITC) is more cytotoxic than triphenyltin isothiocyanate (TPT-ITC) in MCF 7 cells, and the same trend was observed in the MDA-MB-231 cell line. A comet assay revealed the presence of both crosslinks and increasing DNA damage levels after the 17 h treatment with both derivatives. Differences in cytotoxicity of TBT-ITC and TPT-ITC detected by FDA staining correspond to the MTT data, communicating more pronounced effects in MCF 7 than in the MDA-MB-231 cell line. Both derivatives were found to cause apoptosis, as shown by the mitochondrial membrane potential (MMP) depolarization and caspase-3/7 activation. The onset of caspase activation correlated with MMP dissipation and the total cytotoxicity more than with the amount of active caspases. In conclusion, our data suggest that the DNA damage induced by TBT-ITC and TPT-ITC treatment could underlie their cytotoxicity in the cell lines studied.

scholarly journals Beta-Tocotrienol Exhibits More Cytotoxic Effects than Gamma-Tocotrienol on Breast Cancer Cells by Promoting Apoptosis via a P53-Independent PI3-Kinase Dependent Pathway

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 577 ◽  
Author(s):  
Maya Idriss ◽  
Mohammad Hassan Hodroj ◽  
Rajaa Fakhoury ◽  
Sandra Rizk
Keyword(s):  
Breast Cancer ◽  
Vitamin E ◽  
Cell Survival ◽  
Cell Lines ◽  
Breast Adenocarcinoma ◽  
G1 Arrest ◽  
Human Breast ◽  
Gamma Tocotrienol ◽  
Survival Proteins

Studies on tocotrienols have progressively revealed the benefits of these vitamin E isoforms on human health. Beta-tocotrienol (beta-T3) is known to be less available in nature compared to other vitamin E members, which may explain the restricted number of studies on beta-T3. In the present study, we aim to investigate the anti-proliferative effects and the pro-apoptotic mechanisms of beta-T3 on two human breast adenocarcinoma cell lines MDA-MB-231 and MCF7. To assess cell viability, both cell lines were incubated for 24 and 48 h, with different concentrations of beta-T3 and gamma-T3, the latter being a widely studied vitamin E isoform with potent anti-cancerous properties. Cell cycle progression and apoptosis induction upon treatment with various concentrations of the beta-T3 isoform were assessed. The effect of beta-T3 on the expression level of several apoptosis-related proteins p53, cytochrome C, cleaved-PARP-1, Bax, Bcl-2, and caspase-3, in addition to key cell survival proteins p-PI3K and p-GSK-3 α/β was determined using western blot analysis. Beta-tocotrienol exhibited a significantly more potent anti-proliferative effect than gamma-tocotrienol on both cell lines regardless of their hormonal receptor status. Beta-T3 induced a mild G1 arrest on both cell lines, and triggered a mitochondrial stress-mediated apoptotic response in MDA-MB-231 cells. Mechanistically, beta-T3′s anti-neoplastic activity involved the downregulation of phosphorylated PI3K and GSK-3 cell survival proteins. These findings suggest that vitamin E beta-T3 should be considered as a promising anti-cancer agent, more effective than gamma-T3 for treating human breast cancer and deserves to be further studied to investigate its effects in vitro and on other cancer types.

Enhanced susceptibility to apoptosis and growth arrest of human breast carcinoma cells treated with silica nanoparticles loaded with monohydroxy flavone compounds

2019 ◽  
Vol 97 (5) ◽  
pp. 513-525
Author(s):  
Nagwa Abo El-Maali ◽  
Gamal Badr ◽  
Douaa Sayed ◽  
Randa Adam ◽  
Gamal Abd El Wahab
Keyword(s):  
Breast Cancer ◽  
Breast Carcinoma ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Cell Lines ◽  
Breast Cancer Cells ◽  
Growth Arrest ◽  
Human Breast ◽  
Hydroxy Flavone ◽  
Mcf 7

The treatment of drug-resistant cancer is a clinical challenge, hence screening for novel anticancer drugs is critically important. In this study, we investigated the anti-tumor potential of three plant-derived flavone compounds: 3-hydroxy flavone (3-HF), 6-hydroxy flavone (6-HF), and 7-hydroxy flavone (7-HF), either alone or combined with silica nanoparticles (3-HF + NP, 6-HF + NP, and 7-HF + NP), on the human breast carcinoma cell lines MDA-MB-231 and MCF-7, as well as on non-tumorigenic normal breast epithelial cells (MCF-10). The IC50values of these flavone compounds loaded with NP (flavones + NP) in these cell lines were determined to be 1.5 μg/mL without affecting the viability of normal MCF-10 cells. Additionally, using annexin V – propidium iodide double-staining followed by flow cytometry analysis, we found that the combination of flavones with NP significantly induced apoptosis in MCF-7 and MDA-MB-231 cancer cells. Furthermore, flavones + NP increased the expression of cytochrome c and caspase-9, mediating the growth arrest of these cancer cells. Most importantly, the combination of flavones with NP significantly abolished the expression of ATF-3, which is responsible for the proliferation and invasion of bone-metastatic breast cancer cells. Our data revealed the potential therapeutic effects of these flavones in fighting breast cancer cells, and provide the first insights concerning the underlying molecular mechanisms.

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