scholarly journals Hepatoprotective activity of Flacourtia jangomas (Lour.) Raeuschleaves and fruit methanolic extract on paracetamol-induced hepatotoxicity in HepG2 Cells

Biomedicine ◽  
2021 ◽  
Vol 41 (3) ◽  
pp. 587-591
Author(s):  
Akshaya Pai ◽  
Chandrakala Shenoy
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Hepg2 Cell Line ◽  
Fruit Extract ◽  
Deciduous Fruit

Introduction and Aim: Plants have become the current focus of research in treating the various diseases and ailments. Flacourtia jangomas (Lour.) Raeusch belongs to the familySalicaceae. Itis a small deciduous fruit tree having immense nutritional and medicinal significance. Different parts of the plant are pharmaceutically used forcuring various ailments. In this study, we investigated the hepatoprotective activity of Flacourtia jangomas (Lour.) Raeusch leaves and fruit methanolic extract on Paracetamol induced HepG2 cell line.   Methods: The cytotoxic and hepatoprotective properties were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); lipid peroxidation (malondialdehyde (MDA) levels).   Results:The increased cell viability of 140.43± 4.07% and 133.93±3.20%was observed in HepG2 cells treated with methanolic extract of F. jangomas leaf and fruit extract respectively at 10µg/ml concentration and then decreased along with the rise of F. jangomas leaf and fruit extract concentrations. The level of LDH, ALT, AST and MDA decreased after F. jangomas leaf and fruit treatment compared to negative control.   Conclusion: This study suggests that the methanolic Extract of F. jangomas (Lour.) Raeusch leaves(FJL)and fruit (FJF) shows hepatoprotective activity in Paracetamol induced HepG2 cell line by the decrease in AST and ALT activities and LDH and MDA level. Hence, it could be considered as a therapeutic agent in curing liver-related diseases.  

scholarly journals The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hamed Mir ◽  
Daniel Elieh Ali Komi ◽  
Mahdi Pouramir ◽  
Hadi Parsian ◽  
Ali Akbar Moghadamnia ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Leaf Extract ◽  
Dry Weight ◽  
P Value ◽  
Hepg2 Cell Line ◽  
Butyl Hydroperoxide ◽  
Cell Growth Suppression

Abstract Objective In present study, the effects of the leaf extract of Pyrus biossieriana Buhse on tert-Butyl hydroperoxide (t-BHP) induced toxicity in the HepG2 cell line were investigated. Results HepG2 cells were exposed to different concentrations of both extract (1.5, 2.0, and 2.5 mg/mL) and t-BHP (100, 150, and 200 μM). The total flavonoid and phenolic contents, the cell viability, lipid peroxidation, NO generation, and the total antioxidant capacity in cell media were assessed. The amount of arbutin was estimated 12.6% of the dry weight of leaves (equivalent to 126 mg/g). Additionally, the amounts of flavonoids and phenols in extract were estimated 119 mg/g and 418 mg/g, respectively. The cells incubated with t-BHP showed a significant decrease in survival (p < 0.001). Preincubation with extract (1.5 mg/mL and 2.0 mg/mL) attenuated the t-BHP toxicity and increased the cell viability in cells exposed even to the highest concentration of t-BHP (200 μM) (p value < 0.001, and p value = 0.035) respectively. Additionally, treatment with extract reduced the cell growth suppression caused by t-BHP. The P. biossieriana Buhse leaf extract at concentrations of 1.5 and 2.0 mg/mL is capable of attenuating t-BHP-induced cytotoxicity in HepG2 cells.

scholarly journals Curcumin Protects HepG2 Cells from the Cytotoxic Effect of Drugs with Anti-fibrotic Activity

2021 ◽  
Author(s):  
Mariana Y. Medina-Pizaño ◽  
Marina N. Medina-Rosales ◽  
Esperanza Sánchez-Alemán ◽  
Sandra L. Martínez-Hernández ◽  
Liseth R. Aldaba-Muruato ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Morphological Changes ◽  
Liver Parenchyma ◽  
Hepg2 Cell Line ◽  
Secondary Effects ◽  
Hamster Model ◽  
Hematoxylin And Eosin

Abstract Background: The α and β adrenoblockers have been tested as an alternative treatment for chronic liver lesions such as fibrosis and cirrhosis in animal models, as well as their possible participation during the regeneration of the damage caused by liver cirrhosis in a hamster model. However, it was observed that doxazosin caused slight morphological changes in hepatocytes, while that curcumin showed protection to the hepatic parenchyma. Regardless, the pharmacokinetic effects of these 𝛼/𝛽 adrenoblockers on the hepatocytes' cell viability, possibly involved in the hepatic parenchyma's repopulation during cirrhosis reversal, are unknown. The present study aimed to elucidate the protective effect of curcumin on the possible side effects of doxazosin, tamsulosin, and carvedilol on the HepG2 cell line, drugs already tested with antifibrotic activity.Methods: HepG2 cells were exposed to 0.1, 0.5, 10, and 25 µM of doxazosin, carvedilol, and tamsulosin for 24, 48, and 72 h, for curcumin, cells were pretreated with 1 µM for 1 h before exposure to α and β adrenoblockers. The cell viability was assessed by MTT assay. The morphological changes were determined using hematoxylin and eosin (H&E) staining, scanning electron microscope (SEM), and acridine orange (AO) staining. Results: We observed that the doxazosin decreases cell viability dependently time and dose; carvedilol and tamsulosin increase cell proliferation. However, curcumin induces regulation of these effects in HepG2 cell line, increasing or maintaining viability compared to control. The pretreatment with curcumin regulated AST levels (aspartate aminotransferase) and ALT (alanine aminotransferase) in cells exposed to α and β adrenoblockers. The SEM and H&E staining provided evidence that doxazosin, carvedilol, and tamsulosin induced morphological changes in HepG2 cell line, depending on time and dose, approximately 80% of the cells treated with drugs were balonized, and curcumin protected these effects, maintaining the morphology in 90% of the treated cells.Conclusions: The present study demonstrates that curcumin protected the HepG2 cells against cytotoxicity and morphological changes induced by the α and β adrenoblockers attenuating secondary effects for possible oxidative stress. In this way, it is concluded that these treatments with antifibrotic effect, in co-treatment with curcumin, will not affect the possible repopulation process of the liver parenchyma during the reversion of fibrosis.

scholarly journals Differential Effects of Red Yeast Rice, Berberis Aristata and Morus Alba Extracts, Active Components of LopiGLIK®, on PCSK9 in HepG2 Cells

2018 ◽  
Author(s):  
Maria Giovanna Lupo ◽  
Chiara Macchi ◽  
Silvia Marchianò ◽  
Haixia Chen ◽  
Cesare Sirtori ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Promoter Activity ◽  
Fatty Acid Synthase ◽  
Morus Alba ◽  
Red Yeast Rice ◽  
Mrna Levels ◽  
Hepg2 Cell Line ◽  
Red Yeast

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is the key regulator of low-density lipoprotein cholesterol (LDL-C) plasma levels. We previously observed that treatment of dyslipidemic subjects with nutraceutical combination containing red yeast rice (monacolin K 3.3 mg), Berberis aristata cortex extract (Berberine 531.25 mg) and Morus alba leaves extract (1-deoxynojirimycin 4 mg) (LopiGLIK&reg;) did not alter the plasma PCSK9 levels. Thus, the aim of the present study was to investigate the effect of these three components on PCSK9 expression in HepG2 cell line in relationship to their effects on LDL-C cellular uptake. HepG2 cell line were incubated with Berberis aristata cortex extract (BCE), red yeast rice (RYR) and Morus alba leaves extract (MLE) alone or in combination for 24 h. RYR (50 &micro;g/mL) increased PCSK9 protein expression (WB and ELISA assays), PCSK9 mRNA and its promoter activity. BCE (40 &micro;g/mL) reduced PCSK9 expression, mRNA levels and promoter activity. MLE determined a concentration-dependent inhibition of PCSK9 at mRNA and protein levels, with a maximal reduction at 1 mg/mL; no significant changes in PCSK9 promoter activity were found. MLE also downregulates the expression of fatty acid synthase and HMG-CoA reductase mRNA levels. The combination of RYR, BCE and MLE reduced PCSK9 at mRNA, protein, and promoter activity. Finally, this combination induced the LDL receptor and LDL-C uptake by HepG2 cells. In conclusion, the positive effect of MLE on PCSK9 supports the rational of using this nutraceutical combination to control hyperlipidemic conditions.

Novel Health Supplement for Recovery of Alcohol Induced Adverse Effects and Associated Disorders: HepG2 Cells Study and Safety Dose Profiling on Rats

2021 ◽  
Vol 7 (5) ◽  
pp. 1-9
Author(s):  
Neelesh Kumar Nema ◽  
Keyword(s):  
Adverse Effects ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Plant Extracts ◽  
Hepg2 Cells ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Study Objective ◽  
Hepatoma Hepg2

The present study objective was to design and develop novel health-supplement formula from plant extracts and was to evaluate the formula for high episodic alcohol toxicities, and associated disorders against alcohol intoxicated and oxidative damaged Human Hepatoma HepG2 cell line.

Syringic acid triggers reactive oxygen species–mediated cytotoxicity in HepG2 cells

2019 ◽  
Vol 38 (6) ◽  
pp. 694-702 ◽  
Author(s):  
S Gheena ◽  
D Ezhilarasan
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Reactive Oxygen ◽  
Syringic Acid ◽  
Oxygen Species ◽  
Hepg2 Cell Line ◽  
Gene Expressions

Hepatocellular carcinoma is the second most common cause of cancer death in the world and its incidence has dramatically increased worldwide in the past two decades. Syringic acid (SA) has been studied for its hepatoprotective, anti-inflammatory, immunomodulatory, free radical scavenging, and antioxidant activities. We aimed to evaluate the cytotoxic effect of SA against human hepatoma HepG2 cell line. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HepG2 cells were treated with SA at concentration ranges of 25, 50, and 100 µM for 24 h. Reactive oxygen species (ROS) expression was investigated by dichlorofluorescein staining assay. Morphological changes of SA-treated HepG2 cells were evaluated by acridine orange (AO) and ethidium bromide (EB) dual staining. Apoptotic marker gene expressions were evaluated by qPCR. SA treatment caused significant cytotoxicity and liberation of ROS in HepG2 cells. AO and EB staining showed membrane blebbing and distortion in SA-treated cells. Apoptotic markers such as caspases 3 and 9, cytochrome c, Apaf-1, Bax, and p53 gene expressions were significantly increased upon SA treatment indicating the possibility of apoptosis induction in HepG2 cells. This treatment also caused significant downregulation of Bcl-2 gene expression. SA has a cytotoxic effect on human HepG2 cell line, and this might be a promising agent in anticancer research.

scholarly journals Evaluating the Effects of Dithiothreitol and Fructose on Cell Viability and Function of Cryopreserved Primary Rat Hepatocytes and HepG2 Cell Line

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Mahdokht H Aghdai ◽  
Akram Jamshidzadeh ◽  
Mahsa Nematizadeh ◽  
Mahtab Behzadiannia ◽  
Hossein Niknahad ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Rat Hepatocytes ◽  
Hepg2 Cell Line ◽  
Primary Rat Hepatocytes ◽  
And Function

Effects of Bcl-2 and Bcl-XL protein levels on chemoresistance of hepatoblastoma HepG2 cell line

2000 ◽  
Vol 78 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Dan Luo ◽  
Samuel Chak-Sum Cheng ◽  
Hong Xie ◽  
Yong Xie
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Drug Application ◽  
Hepg2 Cell Line ◽  
Trypan Blue Exclusion ◽  
Transfected Cells ◽  
Protein Levels ◽  
Bax Protein ◽  
Significant Difference

The ratio between apoptotic promoters and repressors in the Bcl-2 family determines the chemosensitivity of cells to apoptotic stimuli. This study examines the chemoresistance of a transfected human hepatoblastoma HepG2 cell-line during Taxol and Doxorubicin application. Sense bcl-2, and anti-sense bcl-XL gene fragments were separately inserted into HepG2 cells via stable transfection. The expression profile of the Bcl-2 family proteins was determined by Western blot analysis. Chemosensitivity of the transfected cells was measured by Trypan blue exclusion assay and XTT reduction assay during drug application. In the absence of Bax protein, HepG2 cells with elevated Bcl-2 protein levels did not exhibit any significant increase in chemosensitivity towards the drugs. Transfected cells with reduced Bcl-XL levels became more sensitive to the drugs, and a significant difference in IC50 values was observed. The chemosensitivity of HepG2 cells to Taxol and Doxorubicin was not affected by Bcl-2 levels, while reduction of Bcl-XL levels rendered the cells more sensitive to the drugs. This suggests that the Bcl-2 protein alone could not protect HepG2 cells from drug-induced apoptosis, and that the Bcl-XL protein may be a target for gene therapy in hepatoblastoma treatment. Key words: apoptosis, chemoresistance, Taxol, Doxorubicin, hepatoblastoma.

scholarly journals EVALUATION OF HEPATOPROTECTIVE POTENTIAL OF LEAF AND LEAF CALLUS EXTRACTS OF ANISOCHILUS CARNOSUS (L) WALL.

2018 ◽  
Vol 10 (3) ◽  
pp. 156
Author(s):  
Nissar Ahmad Reshi ◽  
Sudarahana Mysore Shankarsingh ◽  
Girish Hodiyala Vasanaika
Keyword(s):  
Secondary Metabolites ◽  
Cell Viability ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Alcohol Intoxication ◽  
Leaf Explants ◽  
Hepatoprotective Activity ◽  
Phytochemical Analysis ◽  
Toxic Dose ◽  
Viability Percentage

<p>The study was carried out to evaluate the hepatoprotective activity of leaf and leaf callus extracts of <em>Anisochilus carnosus</em> (L) Wall. against alcohol induced toxicity using HepG2 cell line. Leaf explants were cultured on Murashige and Skoog solid medium supplemented with different growth regulators. Prior to the determination of hepatoprotective property leaf and leaf callus extracts were subjected to the toxic dose study. The degree of hepatoprotection of extracts was determined by measuring cell viability percentage by MTT assay. The preliminary phytochemical analysis of leaf and leaf callus was carried out by qualitative analysis. Maximum percentage of callus formation (98%) was obtained in MS medium fortified with 3 mg/l 2,4-D. HepG2 cells were pretreated with the different concentrations (below toxic dose) of leaf and leaf callus extracts for 72 hours followed by alcohol intoxication. Results revealed that ethanolic leaf extract pretreated HepG2 cells show 94% cell viability compared to the standard silymarin pretreated HepG2 cells which showed 81% cell viability. Leaf callus extracts also exhibited significant hepatoprotective activity where ethanolic callus extract pretreated HepG2 cells showed 86% viability after intoxication with alcohol. Results revealed that HepG2 cell viability percentage is dose dependent. Phytochemical studies revealed the presence of different secondary metabolites in leaf and leaf callus extracts. The bio-efficacy study confirms the presence of secondary metabolites of hepatoprotective nature in leaf and leaf callus of <em>A. carnosus.</em></p>

Sign in / Sign up

Export Citation Format

Share Document