scholarly journals Immunohistochemical expression of Cyclooxygenase 2 reflects the proliferative activity in the epithelium of odontogenic lesions

2022 ◽  
Vol 10 (1) ◽  
Author(s):  
Vani Verma ◽  
Chetana Chandrashekar ◽  
Raghu Radhakrishnan ◽  
Monica Charlotte Solomon
Keyword(s):  
Epithelial Cells ◽  
Dentigerous Cyst ◽  
Cyclooxygenase 2 ◽  
Odontogenic Keratocyst ◽  
Odontogenic Cysts ◽  
Radicular Cyst ◽  
Chi Square ◽  
Ki 67 ◽  
Odontogenic Epithelium ◽  
Cox 2

Purpose:  Odontogenic cysts and tumors comprise a major component of lesions of the oral and maxillofacial region. The pathogenesis of these lesions involves the interaction between the odontogenic epithelium and the ectomesenchyme. However, the clinical behavior of these biological entities is unpredictable. The aim of this study was to evaluate the role of Cyclooxygenase 2 (COX-2) in the pathogenesis and prognostication of odontogenic lesions.Material and method:  : In this study formalin-fixed paraffin-embedded tissue section of Odontogenic Keratocyst (n=10) Dentigerous cyst (n=10), Radicular cyst (n=10) and unicystic ameloblastoma (n=10) were immunohistochemically stained with COX-2 (NCL2-COX-2- 4H12) and with Ki 67 (Ki-67 GM001) using standard staining protocols. The cytoplasmic expression of COX-2 in all the lesions was semi-quantitatively assessed. The pattern of expression of COX-2 among the different odontogenic lesions was statistical analyzed using the ANOVA test and the chi-square test.Results: All the 40 odontogenic lesions that were evaluated expressed COX-2 immunohistochemically. A high number of odontogenic epithelial cells expressed COX-2 in most of the odontogenic keratocyst, radicular cyst and unicystic ameloblastomas. The expression of COX-2 was significantly (p=0.036) higher in Unicystic Ameloblastomas and Radicular cyst compared to that of Odontogenic Keratocyst and the dentigerous cyst.Conclusion: The recognition that expression of COX-2 by odontogenic epithelial cells may indeed shed a new light on the biological mechanisms involved in the development of these benign yet aggressive lesions of the jaws. An insight into the molecular interactions occurring in the odontogenic epithelium will aid in better management of these lesions. 

Contribution of stromal and epithelial cells to cyclooxygenase-2 (COX-2) activity in Barrett's esophagus

2001 ◽  
Vol 120 (5) ◽  
pp. A78-A79
Author(s):  
N BUTTAR ◽  
K WANG ◽  
M ANDERSON ◽  
L LUTZKE ◽  
K KRISHNADATH
Keyword(s):  
Epithelial Cells ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Cyclooxygenase 2 ◽  
Cox 2

Cyclooxygenase-2 Expression Is Related With Localization, Proliferation, and Overall Survival in Canine Melanocytic Neoplasms

2011 ◽  
Vol 48 (6) ◽  
pp. 1204-1211 ◽  
Author(s):  
C. M. Martínez ◽  
C. Peñafiel-Verdú ◽  
M. Vilafranca ◽  
G. Ramírez ◽  
M. Méndez-Gallego ◽  
...  
Keyword(s):  
Overall Survival ◽  
Mitotic Index ◽  
Tumor Location ◽  
Cyclooxygenase 2 ◽  
Ki 67 ◽  
Melanocytic Tumors ◽  
Oral Neoplasms ◽  
Survival Frequency ◽  
Cox 2 ◽  
Proliferative Indices

A direct relationship has been firmly established between cyclooxygenase-2 (COX-2) expression and malignant behavior in human melanoma. This report examines the relationship between COX-2 expression and tumor location, mitotic and proliferative indices, degree of T CD3+ lymphocyte infiltration, overall survival, and frequency of recurrence and metastasis of 57 melanocytic tumors (25 oral and 32 cutaneous). COX-2 was highly or moderately expressed in 88% of oral neoplasms (22 of 25), whereas for their cutaneous counterparts, COX-2 expression was low or insignificant in 75% of cases (24 of 32). High and moderate COX-2 expression levels were observed in 73% of melanocytic tumors with a mitotic index ≥ 3 per 10 high-power fields (26 of 36), whereas in 81% of tumors with a mitotic index < 3 (17 of 21), expression was mild or absent. There were 41 cases with known clinical outcomes; of those showing high, moderate, and mild COX-2 expression, 83.3% (10 of 12), 37.5% (3 of 8), and 25% (2 of 8) died, respectively, whereas 100% of animals showing no COX-2 expression (13 of 13) were still alive at the last follow-up. COX-2 expression was statistically correlated with tumor location, mitotic and percentage Ki-67 proliferative indices, and overall survival, frequency of neoplastic recurrence and metastasis. Regression analysis also showed disease-specific predictive value for COX-2 expression for subjects with melanocytic neoplasms. Additionally, only high COX-2 expression showed significant differences in overall survival, in comparison with moderate, mild, or absent expression. These results suggest that high COX-2 expression may be considered a prognostic biomarker and potentially as a target for therapeutic and preventive strategies in canine melanocytic neoplasms.

scholarly journals Despite Transcriptional and Functional Coordination, Cyclooxygenase-2 and Microsomal Prostaglandin E Synthase-1 Largely Reside in Distinct Lipid Microdomains in WISH Epithelial Cells

2005 ◽  
Vol 53 (11) ◽  
pp. 1391-1401 ◽  
Author(s):  
William E. Ackerman IV ◽  
John M. Robinson ◽  
Douglas A. Kniss
Keyword(s):  
High Resolution ◽  
Epithelial Cells ◽  
Cyclooxygenase 2 ◽  
Prostaglandin E ◽  
Potential Mechanism ◽  
Prostaglandin E Synthase ◽  
Lipid Microdomains ◽  
Cox 2 ◽  
Cytokine Stimulation ◽  
Soluble Fractions

Cytokine-induced prostaglandin (PG)E2 synthesis requires increased expression of cyclooxygenase-2 (COX-2) in human WISH epithelial cells. Recently, an inducible downstream PGE synthase (microsomal PGE synthase-1, mPGES-1) has been implicated in this inflammatory pathway. We evaluated cooperation between COX-2 and mPGES-1 as a potential mechanism for induced PGE2 production in WISH cells. Cytokine stimulation led to increased expression of both enzymes. Selective pharmacological inhibition of these enzymes demonstrated that induced PGE2 release occurred through a dominant COX-2/mPGES-1 pathway. Unexpectedly, immunofluorescent microscopy revealed that the expression of these enzymes was not tightly coordinated among cells after cytokine challenge. Within cells expressing high levels of both mPGES-1 and COX-2, immunolabeling of high-resolution semithin cryosections revealed that COX-2 and mPGES-1 were largely segregated to distinct regions within continuous intracellular membranes. Using biochemical means, it was further revealed that the majority of mPGES-1 resided within detergent-insoluble membrane fractions, whereas COX-2 was found only in detergent-soluble fractions. We conclude that although mPGES-1 and COX-2 show transcriptional and functional coordination in cytokine-induced PGE2 synthesis, complementary morphological and biochemical data suggest that a majority of intracellular mPGES-1 and COX-2 are segregated to discrete lipid microdomains in WISH epithelial cells.

scholarly journals Immunohistochemical expression of ki-67 in odontogenic keratocyst: Evidence of aggressive behavior.

2020 ◽  
Vol 27 (01) ◽  
pp. 74-79
Author(s):  
Rabiya Saif ◽  
Hafiz Majid Jehangir ◽  
Abdul Hannan Nagi ◽  
Nadia Naseem ◽  
Zainab Rizvi ◽  
...  
Keyword(s):  
Aggressive Behavior ◽  
Descriptive Study ◽  
World Health ◽  
Odontogenic Keratocyst ◽  
High Recurrence Rate ◽  
Odontogenic Cysts ◽  
P Value ◽  
Proliferative Potential ◽  
Nuclear Staining ◽  
Ki 67

The odontogenic keratocyst (OKC) well-known for its aggressiveness and high recurrence rate, comprises approximately 11% of all jaw cysts. Due to its aggressive behavior it was placed into category of tumour in 2005 by the World Health Organization (WHO). Objectives: The purpose of this study was to determine the Ki-67 expression in Odontogenic Keratocysts to predict its proliferative potential. Study Design: Descriptive study. Setting: Department of Morbid Anatomy and Histopathology, UHS. Periods: June 2014- June 2018. Material & Methods: This is a descriptive study comprising of 39 cases of odontogenic cysts. These surgically removed samples were processed at University of Health Sciences (UHS) laboratory. Routine staining with Hematoxylin & Eosin stain along with immunohistochemistry (IHC) with Ki-67 antibody was performed. Immunohisto chemical scoring was done on the basis of percentage of the nuclear staining of Ki-67. Data was entered into SPSS 22 and descriptive statistics were measured in the form of percentage and frequency. Quantitative variables such as age of patient, size of the cyst, and Ki-67 score were also measured. P value <0.05 was taken as significant. Results: The mean age of the patients was 25.08 ±14.5 years. Significant association was observed between histological variables with odontogenic keratocyst such as parakeratinized epithelial lining (p = 0.00), epithelial hyperplasia both typical and atypical (p = 0.02) and focal spongiosis (p = 0.04). Foci having epithelial atypia demonstrated stronger staining intensity compared to adjacent normal epithelium. However, no significant association was observed between the histological variables and Ki-67 expression. Conclusion: OKC expressed low Ki-67 expression in most of the cases, however, foci of strong expression were also observed in few cases.

scholarly journals Immunohistochemical Expression of CDC7 in Dentigerous Cyst, Odontogenic Keratocyst and Radicular Cyst

2018 ◽  
Vol 61 (1) ◽  
pp. 17-21
Author(s):  
Zohreh Jaafari-Ashkavandi ◽  
Ahmad Alipour Tuyeh ◽  
Sepideh Assar
Keyword(s):  
Dentigerous Cyst ◽  
Odontogenic Cysts ◽  
Radicular Cyst ◽  
Immunohistochemical Expression ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Dentigerous Cysts ◽  
Pathologic Lesions ◽  
The Mean ◽  
Post Hoc

CDC7 is a serine/threonine kinase which has an essential role in initiation of DNA proliferation and S phase. It increases the invasion and proliferation in many pathologic lesions. This study aimed to evaluate the expression of CDC7 in the most common odontogenic cysts. We evaluated 17 dentigerous cysts, 18 odontogenic keratocysts (OKC) and 13 radicular cysts immunohistochemically. The mean expression of CDC7 was analyzed using ANOVA and Post-HOC methods. All specimens revealed CDC7 expression. Higher expression of CDC7 in OKC and radicular cyst was shown in comparison to dentigerous cyst (P < 0.001), while radicular cyst and OKC groups showed no difference in CDC7 expression (P = 0.738). The high expression of CDC7 in OKC suggests that this protein could be related to the higher proliferation rate and invasiveness of OKC. On the other hand, the higher CDC7 expression in radicular cyst may simply be related to inflammation as this cyst is neither aggressive nor invasive.

scholarly journals Evaluation of Microvessel Density in Odontogenic Keratocyst, Dentigerous Cyst and Radicular Cyst

2021 ◽  
pp. 96-100
Author(s):  
Premkumar ◽  
Maya Ramesh ◽  
Mathew Jacob ◽  
B Sekar ◽  
K. Indrapridharshini ◽  
...  
Keyword(s):  
Microvessel Density ◽  
Dentigerous Cyst ◽  
Odontogenic Keratocyst ◽  
Radicular Cyst

scholarly journals Correlation cyclooxygenase-2 expression and histopathological grading in locally advanced breast cancer

2019 ◽  
Vol 7 (7) ◽  
pp. 2595
Author(s):  
Yasser Maulidan ◽  
Salman A. Syamsu ◽  
Rudy Thabry ◽  
Zainal Abidin
Keyword(s):  
Breast Cancer ◽  
Advanced Breast Cancer ◽  
Locally Advanced Breast Cancer ◽  
Locally Advanced ◽  
Cyclooxygenase 2 ◽  
Advanced Breast ◽  
Breast Cancer Patients ◽  
Chi Square ◽  
Cox 2 ◽  
Histopathological Grading

Background: Cyclooxygenase-2 (COX-2) is involved in the carcinogenesis process and tumor progression into cancer. It has been reported recently, there was a COX-2 expression at breast cancer. Patients with a high level of COX-2 expression can have a local recurrence and decrease life expectancy, and an increase of COX-2 expression in tissue likelihood has prognostic value. This study aims to correlation cyclooxygenase-2 (cox-2) expression and histopathological grading in locally advanced breast cancer patient in AW Sjahranie Hospital in Samarinda city.Methods: This study was an observational study with crossectional design to analysis the correlation COX-2 expression and histopathological grading in patients with locally advanced breast cancer and using data analysis with the Chi-Square test.Results: The results showed that the correlation was not significant (P>0.05), where there was no correlation of COX-2 expression with histopathological grading (P=0.221) and there was no correlation with the last condition of patients (P=0.61). Although patients with breast cancer high grade and moderate grade percentages were significantly higher in positive COX-2 than in COX-2 negative expression.Conclusions: There were no correlation between COX-2 expression and histopathological grading and there was no significant relationship between COX-2 expression and the last condition, as evidenced by the statistical test results showing that the differences were not significant.

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