scholarly journals ASSOCIATON OF KAPPA-CASEIN GENOTYPE AND THE LINEAR PARAMETER IN TWO INDIGENIOUS BOS INDICUS AND BOS TAURUS CATTLE IN NIGERIA

2020 ◽  
Vol 1 (1) ◽  
pp. 12-22
Author(s):  
B. M. Olanrewaju ◽  
E. B. Oghate ◽  
A. J. Adetunb ◽  
O. OlawaleJ ◽  
A. C Chineke

Kappa-casein as a mammalian milk protein is involved in a several important physiological processes and it’s about 80% of the total protein in cow milk. This study aimed at genotyping bovine Kappa casein (CSN3) in two indigenous Nigerian cattle populations and to determine the frequency distribution of Kappa casein variants as detected across the animals examined and their association with the body measurement. DNA was extracted from 100 blood samples of 50 White Fulani and 50 N’dama cattle for identification and genotyping of kappa-casein gene by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) test using HindIII restriction endonucleases. The PCR products of the specific primer K-F and K-R for the two cattle breeds give 530bp specific band. Digestion of 530bp amplified products of White Fulani and N’dama by restriction endonuclease HindIII generated three fragments of 530-, 370- and 160- bp each for the two breeds. Results of the cuts with this enzyme show the presence of genotypes AA, AB and BB in the samples. These findings suggest that BB genotype could be selected for increase body conformation and protein content of milk. Olanrewaju, B. M. | Department of Animal Production and Health, The Federal University of Technology Akure (FUTA), Ondo State, Nigeria.

Author(s):  
Ashish Ranjan ◽  
K. Raja ◽  
Ranjana Sinha ◽  
I. Ganguly ◽  
I. Gupta ◽  
...  

Present study was conducted on 50 bulls and 40 male calves of Sahiwal, Tharparkar and Karan Fries cattle maintained at ABRC and LRC, NDRI Karnal (Haryana) to characterize and identify genetic polymorphisms in TNP1 gene. A total of 1568 bp region of TNP-1 gene includes 490 bp of promoter region and two exon and one intron was sequenced and characterized in Bos indicus cattle breeds which are widely distributed in Indian sub-continent. Four sets of primers for TNP1 gene on the basis of Bos Taurus sequence (Acc. No- BK_006511) were designed using Primer3 software and PCR products of 487, 450, 455 and 250 bp were obtained. Amplicons were custom sequenced and subjected to Clustal W analysis which showed no nucleotide changes in coding region and non coding region in Indian cattle breeds as compared to Bos taurus. The 490 bp of promoter region was subjected to transcription factor binding site. Three TATA boxes and two CAAT boxes were identified in the studied fragment. Analysis of SNP was performed using restriction fragment length polymorphism (PCR-RFLP), to detect nucleotide changes in the sequence as reported (g.528G>A, SS1388116558) in Chinese Holstein breed. No polymorphisms were found for tested SNP. Only one genotype GG indicates the absence of variability in the sampled population.


2019 ◽  
Vol 26 (1) ◽  
pp. 44
Author(s):  
Endang Tri Margawati ◽  
Slamet Diah Volkandari ◽  
Indriawati Indriawati ◽  
Emma M. Svensson

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.


Author(s):  
Izz Nurryn Mohd Riza ◽  
Nur Adlin Wahida Murad ◽  
Liyana Amalina Adnan

Milks are dairy products that consists of high nutritional value which is good for health and provides energy to the body. Besides, milk also is believed to have antioxidant properties which helps to reduce the oxidative stress that may cause many diseases. Generally, cow milks are the most popular amongst all the dairy products as it is affordable and easy to find. However, dairy products that are originated from cow contains protein that can induce allergen reactions to some people. Thus, there is milk produced from the plant-based which is soybean that can be used to replace cow milk as it is believed to have similar nutritional value to cow milk. Therefore, to prove its similarity, an identification of antioxidant compounds was performed on soybean milk and cow milk extract using Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The extraction was done by using liquid-liquid extraction method and the excess solvent was removed by using rotary evaporator machine. As a result, soybean milk has been identified to have the similar antioxidant compounds to cow milk. It can be proven with the presence of functional group that are similar to tryptophan and tyrosine which are both existed in cow milk. Hence, this research is essential for community as it contribute maximum benefit for better healthcare.


1997 ◽  
Vol 21 ◽  
pp. 35-42 ◽  
Author(s):  
T. A. Mohammed

SummaryHassawi cattle breed is a mix of Bos indicus and Bos taurus. The cattle are raised in the Eastern province of the country by farming families in mixed farming system. The breed numbers are declining very fast, from 10 449 head in 1986 to an estimated maximum of 4 500 head at present.The decrease is mainly due to replacement by exotic breeds, the indiscriminate crossing with these exotics, particularly in view of the scarcity of the Hassawi bulls for mating. Animals are small in size, mature body weight 210-270 kg for bulls and 150-200 kg for cows, quite uniform in colour (light red) and body conformation have conspicuously reduced dewlap and umbilical folds and relatively large hump. Animals are heat tolerant, sustain high feed intake under ambient temperature, resistant to many diseases prevailing in the region and cows have good mothering ability. Productivity of the breed in terms of meat and milk is low when compared to that of exotics in high input production environments, but reproduction performance excels that of temperate breeds and zebu cattle.Efforts should be made to stop the decline in the breed numbers and to conserve the breed as an asset for production under harsh environment.


1975 ◽  
Vol 20 (1) ◽  
pp. 63-68 ◽  
Author(s):  
S. F. Amakiri ◽  
R. Mordi

SUMMARYThe rate of cutaneous evaporation was highest in the White Fulani (Bos indicus) followed by the N'Dama and German Friesian (Bos taunts). The lowest rate of evaporation was recorded in the Muturu (Bos taurus brachyceros). In all breeds, the rate at 15.00 hr was significantly higher than at 18.00 hr.For the sites sampled, evaporation rate was highest on the hump and mid-side, followed by the neck and lateral thigh and lowest on the dewlap and navel flap.The possible correlation of these results with the size and distribution of the sweat glands on various sites of different cattle breeds is discussed.


1966 ◽  
Vol 2 (2) ◽  
pp. 81-88 ◽  
Author(s):  
J. Oliver

SummaryThe Mashona cattle of Rhodesia are a strain of the Sanga type, which is found in specific areas of Central and Southern Africa. They are descended from protogenic Bos taurus, Bos indicus and Bos brachyceros cattle which were cross-bred in ancient times. in spite of the pandemics which ravaged herds at the turn of the last century, it seems that Mashona cattle have changed little since first brought to the country. An account is given of the Central African environment and the way it affects the physiological processes of cattle. Because Mashona cattle have lived in the Rhodesian environment for many centurites they are well adapted to it. A description is given of Mashona cattle which are being developed as a distinct breed in Rhodesia.


2013 ◽  
Vol 152 (3) ◽  
pp. 464-482 ◽  
Author(s):  
V. A. THOMPSON ◽  
L. G. BARIONI ◽  
T. R. RUMSEY ◽  
J. G. FADEL ◽  
R. D. SAINZ

SUMMARYThe dynamic model presented in the current paper estimates heat production and heat flow between growing and mature cattle (Bos indicus and Bos taurus) and the surrounding environment. Heat production was calculated using the NRC (2000) and heat flows between the animal and the environment were based largely on existing models and physical principles. Heat flows among the body core, the skin, the coat and the environment were calculated. Heat flows from and to the environment included solar radiation, long wave radiation, convection and evaporative heat loss. Physiological responses of cattle (sweating, panting and vasodilation) were modelled through mechanistic equations. The model required weather (radiation, temperature, wind and vapour pressure), animal (body-core weight and genotype-specific parameters) and dietary inputs (dry matter intake rates and diet composition) and estimated heat balance and the physiological responses of the animal to within-day weather variation. The current paper has focused on heat stress, although the model was designed to run under both hot and cold climatic conditions. The model developed in the current paper provides researchers and livestock producers with the ability to predict heat stress and to evaluate mitigating procedures.


2008 ◽  
Vol 51 (3) ◽  
pp. 298-299 ◽  
Author(s):  
A. Ratna Kumari ◽  
K. M. Singh ◽  
K. J. Soni ◽  
R. K. Patel ◽  
J. B. Chauhan ◽  
...  

Abstract. In mammals, especially dairy cattle the prolactin has important functions like the development of mammary gland affecting milk yield and composition. It has been mapped to chromosome 23 in Bovine (HALLERMAN et al., 1988). A silent A→G transition mutation at the codon for amino acid 103 in exon 3 of bovine prolactin (bPRL) gene gives rise to a polymorphic Rsa I site, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (MITRA et al., 1995; CHRENEK et al., 1998; DYBUS, 2005). The present study reports on the genotype frequencies observed in various Bos taurus and Bos indicus dairy cattle breeds.


2010 ◽  
Vol 9 (11) ◽  
pp. 1660-1663
Author(s):  
Reza Nahavandi ◽  
Nader Asadzadeh ◽  
Adbdolreza Soleimani ◽  
S.M. Nurul Amin ◽  
Parivash Hafezamini ◽  
...  

2008 ◽  
Vol 43 (5) ◽  
pp. 657-659 ◽  
Author(s):  
Rogério Abdallah Curi ◽  
Luis Artur Loyola Chardulo ◽  
Antônio Carlos Silveira ◽  
Henrique Nunes de Oliveira

The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.


Sign in / Sign up

Export Citation Format

Share Document