scholarly journals Isolation and Characterization of Plant Growth Promoting Rhizobacteria for Growth Promotion of Rice (Oryza sativa L.) and Wheat (Triticum aestivum)

2021 ◽  
Vol 2 (2) ◽  
pp. 177-194
Author(s):  
Muhammad Ayyaz ◽  
Zulqurnain Khan ◽  
Nabila Tabassam ◽  
Tariq Sultan ◽  
Amjad Saeed ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Oryza Sativa L ◽  
Growth Enhancement ◽  
Root Weight ◽  
Bacterial Strains ◽  
Phosphorus Solubilization ◽  
Isolation And Characterization

The use of rhizobacteria for plant growth enhancement is decades old. Still, in this era of the 21st century, biofertilizers have become the need of the day due to the health and environmental concerns associated with chemical fertilizers and pesticides. Rhizobacteria strains were isolated from the rhizosphere of rice and wheat. The selected bacterial strains' nitrogen fixation, indole acetic acid (IAA) production, phosphorus solubilization, and antifungal activity were determined on morphological, biochemical, and molecular levels. Production of IAA ranged from 6 µg/ml to 29.33 µg/ml. Ethylene production (C2H4/hr) varied from 2 µmoles to 9.8 µmoles. Maximum Phosphorus Solubilization index (7), decrease in pH (4) and Solubilization % age (0.49) was observed in WM-2 (wheat microbe). Promising results were obtained concerning antifungal activity against Rhizoctonia solani and Fusarium sp. The effect of the potential PGPR strains on the germination of rice and wheat was significantly positive in Petri plates. In the case of rice, the highest shoot length (29.27 cm) was observed by inoculation with RPR-33 (Rice isolate), and the most increased root length (9.33 cm) was observed in the treatment inoculated with RPR-42. The highest shoot fresh weight (476.67 mg/plant) was recorded in the treatment inoculated with RPR-42. The maximum root weight was 170 mg/plant in the same treatment. For wheat, all recorded growth parameters were improved significantly by wheat microbe WM-5. All the PGPR isolates showed positive results for growth parameters of wheat and rice on inoculation. So, it is suggested that these PGPR isolates may be used in potential biofertilizers.

scholarly journals Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

2021 ◽  
Vol 8 (sp1) ◽  
pp. 17-24
Author(s):  
Tanvir Kaur ◽  
Rubee Devi ◽  
Divjot Kour ◽  
Ashok Yadav ◽  
Ajar Nath Yadav
Keyword(s):  
Plant Growth ◽  
Environmental Sustainability ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Sugar Content ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

scholarly journals Impact of Pseudomonas spp. on Plant Growth, Lytic Enzymes and Secondary Metabolites Production

2021 ◽  
Vol 3 ◽  
Author(s):  
Rajesh R. Waghunde ◽  
Ambalal N. Sabalpara
Keyword(s):  
Plant Growth ◽  
Acid Production ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Root Weight ◽  
Bacterial Strains ◽  
Lytic Enzymes ◽  
Acetic Acid Production ◽  
Metabolites Production

Seven strains of Pseudomonas spp. were isolated from the south Gujarat region of India. Antifungal and bacterial activities of bacterial strains were evaluated against important plant pathogens in vitro, among them, PaRS was found most effective. The indole acetic acid production was recorded in all isolated Pseudomonas spp. Seed treatment with PaRS at 6 g/kg was significantly superior over other treatments for plant growth promotion (germination, shoot and root length, shoot and root weight, vigor index, and both shoot and root colonization) under standard roll towel method and pot conditions. The maximum siderophore production was observed in PaRS and medium production in PfRB, PaNS, and PfNC. PaNS and PaRS strains recorded strong HCN production but moderate production recorded in PaWP, PaWS, and PfNC. The maximum phosphate solubalization zone (22 mm) was found in PaRS. PaRS recorded maximum chitinase, ß-1,3-glucanase activity, hydrogen cyanide, and salicylic acid production as compared to other strains.

scholarly journals Assessing the Impact of Biologically Synthesized ZnO Nanoparticles as Source of Nutrient on the Growth of Zea mays L.

2020 ◽  
Author(s):  
Sumera Sabir ◽  
Muhammad Asif Zahoor ◽  
Muhammad waseem ◽  
Muhammad Hussnain Siddique ◽  
Muhammad Shafique ◽  
...  
Keyword(s):  
Zea Mays ◽  
Electron Microscope ◽  
Plant Growth ◽  
Zea Mays L ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Growth Enhancement ◽  
Zno Nps ◽  
Alternate Source ◽  
The Impact

Abstract Nano-fertilizer(s), an emerging field of agriculture, is alternate option for enhancement of plant growth replacing the synthetic fertilizers. For example, zinc oxide nanoparticles (ZnO NPs) can be used as the zinc source for plants. The present investigation was carried out to assess the role of ZnO NPs in growth promotion of maize plants. ZnO NPs were synthesized using Bacillus sp ., which were characterized using Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM) and X-ray diffraction (XRD). The different concentrations of ZnO NPs ( i . e . 0, 2, 4, 6, 8, 16 mg/L) for growth enhancement of maize ( Zea mays L.) were explored in pot culture experiment. It was observed that size of ZnO NPs ranged between 16 and 20 nm. A significant increase in growth parameters like shoot length (61.7 %), root length (56.9 %) and significantly higher level of protein was observed in the treated plants as compared to control The overall pattern for growth biomarkers including the protein contents was maximum at 8mg/L of ZnO NPs. It was observed that application of biologically synthesized ZnO NPs has improved majority of growth biomarkers including plant growth parameters, protein contents and leaf area. All these parameters were positively influenced by ZnO NPs. Therefore, biosynthesized ZnO NPs could be considered as an alternate source of nutrient in Zn deficient soils for promoting the modern agriculture.

scholarly journals Growth Promotion of Rapeseed (Brassica napus L.) and Blackleg Disease (Leptosphaeria maculans) Suppression Mediated by Endophytic Bacteria

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1966
Author(s):  
Nikola Lipková ◽  
Juraj Medo ◽  
Renata Artimová ◽  
Jana Maková ◽  
Jana Petrová ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Leptosphaeria Maculans ◽  
Good Growth ◽  
Blackleg Disease ◽  
Bacterial Strains ◽  
Brassica Napus L ◽  
Pgp Traits

Rapeseed is an important oil crop strongly dependent on high agrochemical inputs. Some pathogens, including Leptosphaeria maculans, cause blackleg disease and can drastically decrease yields. Microbial inoculants seem to be a promising solution to these problems. However, a selection of potent bacterial strains able to improve growth and/or suppress disease is needed. Endophytic bacteria (n = 38) isolated from rapeseed plants with exceptionally good growth were screened for plant growth promoting (PGP) traits and L. maculans antifungal activity. A majority of isolates (35) showed the ability to produce siderophores, 17 isolates solubilized phosphate, and 28 isolates inhibited the growth of L. maculans. The six most promising isolates belonging to Bacillus genera were characterized in detail and compared to two previously published PGP strains. Plant growth measured as total weight and root length of rapeseed seedlings was stimulated by all isolates in comparison to control. The best isolate, 1L6, preliminary identified as Bacillus pumilus showed the highest phosphate solubilization, IAA and HCN production, and growth promotion of plants. Isolates with high antifungal activity in screening showed good potential to suppress disease on plants, with 87% reduction of lesions caused by L. maculans. These strains are good candidates to be explored under field use either solely or in combination.

scholarly journals Isolation and Characterization of Rhizobacteria for their Many Activities Improving the Plant Growth

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Silini ALLAOUA ◽  
Cherif HAFSA ◽  
Ghoul MOSTEFA
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacterial Strains ◽  
High Concentration ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Positive Effect

The use of microbial technology in agriculture extends currently relatively quickly with the identification of new more efficient bacterial strains in the growth of plants. The rhizobacteria that promote the growth of plants (PGPR, plant growth promoting rhizobacteria) can have a positive effect on the productivity of crops( Chaiharn et al., 2008) especially when subjected to salt stress ( Tripathi et al., 1998). Indeed, the salinity considerably affects the crop yield. Inoculating PGPR can mitigate the effects of this type of stress by direct or indirect mechanisms (Ahmad et al., 2008). To select effective strains and having multiple activities, a total of 22 bacterial strains are isolated from different soils. These strains are characterized by their ability to fix nitrogen (nitrogen-free medium culture). They are also tested in vitro for their activities of improvement of plant growth such as the production of the indole acetic acid (IAA) of hydrogen cyanide (HCN), of the siderophore, ammonia (NH3), of solubilization of phosphates and antifungal activity. More than 70% of the strains produce NH3, 54% solubilized more P2O5 10µg/ml and the same rate and has antifungal activity against ≥ to three fungal strains. However, 36% produce more 80µg/ml of IAA, 27% produce siderophores and only two strains produced of HCN. The production of IAA of eight (08) strains is evaluated in the presence of 100, 200 and 300 mM NaCl. The remaining active strains with high concentration of NaCl would be the most effective and can be considered as biofertilizers for improving plant growth in areas affected by salinity.

scholarly journals Optimizing hydroponic culture media and NO3−/NH4+ ratio for improving essential oil compositions of purple coneflower (Echinacea purpurea L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Abbas Samadi ◽  
Ebrahim Sepehr ◽  
Amir Rahimi ◽  
Sergey Shabala
Keyword(s):  
Particle Size ◽  
Essential Oil ◽  
Plant Growth ◽  
Plant Height ◽  
Growth Parameters ◽  
Echinacea Purpurea ◽  
Peat Moss ◽  
Root Weight ◽  
Total Biomass ◽  
Growing Media

AbstractMedicinal plants represent a valuable commodity due to beneficial effects of their natural products on human health, prompting a need for finding a way to optimize/increase their production. In this study, a novel growing media with various perlite particle size and its mixture with peat moss was tested for hydroponic-based production of Echinacea purpurea medicinal plant under greenhouse conditions. The plant growth parameters such as plant height, total fresh leave weight, fresh root weight, total biomass, total chlorophyll, leaf area, and essential oil compositions were assessed. Perlite particle size in the growing media was varied from very coarse (more than 2 mm) to very fine (less than 0.5 mm), and the ratio between perlite and peat moss varied from 50:50 v/v to 30:70 v/v. In addition, two nitrate (NO3−) to ammonium (NH4+) ratios (90:10 and 70:30) were tested for each growing media. The medium containing very fine-grade perlite and 50:50 v/v perlite to peat moss ratio was found to be most optimal and beneficial for E. purpurea performance, resulting in maximal plant height, fresh and dry weight, leaf surface area, and chlorophyll content. It was also found that an increase in NO3−/NH4+ ratio caused a significant increase in plant growth parameters and increase the plant essential oil content. The major terpene hydrocarbons found in extract of E. purpurea with the best growth parameters were germacrene D (51%), myrcene (15%), α-pinene (12%), β-caryophyllene (11%), and 1-Pentadecene (4.4%), respectively. The percentages of these terpene hydrocarbons were increased by increasing of NO3−/NH4+ ratio. It can be concluded that decreasing the perlite particle size and increasing the NO3−/NH4+ ratio increased the plant growth parameters and essential oil compositions in E. purpurea.

scholarly journals Effect of Co-Inoculation of Bacillus sp. Strain with Bacterial Endophytes on Plant Growth and Colonization in Tomato Plant (Solanum lycopersicum)

2021 ◽  
Vol 12 (2) ◽  
pp. 480-490
Author(s):  
Ahsanul Salehin ◽  
Ramesh Raj Puri ◽  
Md Hafizur Rahman Hafiz ◽  
Kazuhito Itoh
Keyword(s):  
Plant Growth ◽  
Solanum Lycopersicum ◽  
Plant Growth Promotion ◽  
Tomato Plant ◽  
Growth Promotion ◽  
Growth Parameters ◽  
The Other ◽  
Time Interval ◽  
Bacillus Sp ◽  
F 33

Colonization of a biofertilizer Bacillus sp. OYK strain, which was isolated from a soil, was compared with three rhizospheric and endophytic Bacillus sp. strains to evaluate the colonization potential of the Bacillus sp. strains with a different origin. Surface-sterilized seeds of tomato (Solanum lycopersicum L. cv. Chika) were sown in the sterilized vermiculite, and four Bacillus sp. strains were each inoculated onto the seed zone. After cultivation in a phytotron, plant growth parameters and populations of the inoculants in the root, shoot, and rhizosphere were determined. In addition, effects of co-inoculation and time interval inoculation of Bacillus sp. F-33 with the other endophytes were examined. All Bacillus sp. strains promoted plant growth except for Bacillus sp. RF-37, and populations of the rhizospheric and endophytic Bacillus sp. strains were 1.4–2.8 orders higher in the tomato plant than that of Bacillus sp. OYK. The plant growth promotion by Bacillus sp. F-33 was reduced by co-inoculation with the other endophytic strains: Klebsiella sp. Sal 1, Enterobacter sp. Sal 3, and Herbaspirillum sp. Sal 6., though the population of Bacillus sp. F-33 maintained or slightly decreased. When Klebsiella sp. Sal 1 was inoculated after Bacillus sp. F-33, the plant growth-promoting effects by Bacillus sp. F-33 were reduced without a reduction of its population, while when Bacillus sp. F-33 was inoculated after Klebsiella sp. Sal 1, the effects were increased in spite of the reduction of its population. Klebsiella sp. Sal 1 colonized dominantly under both conditions. The higher population of rhizospheric and endophytic Bacillus sp. in the plant suggests the importance of the origin of the strains for their colonization. The plant growth promotion and colonization potentials were independently affected by the co-existing microorganisms.

scholarly journals Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

2015 ◽  
Vol 3 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Mohamed A.M. El-Awady ◽  
Mohamed M. Hassan ◽  
Yassin M. Al-Sodany
Keyword(s):  
Nitrogen Fixation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Growth Promotion ◽  
Exponential Growth Phase ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Isolation And Characterization ◽  
Growth Promoting

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

scholarly journals The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 888
Author(s):  
Giorgia Novello ◽  
Patrizia Cesaro ◽  
Elisa Bona ◽  
Nadia Massa ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Global Market ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Mineral Concentration ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Onion Cultivar ◽  
Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

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