scholarly journals Phytochemical evaluation and antioxidant activities in flower and leaves of Cassia fistula and Terminalia arjuna

2021 ◽  
Vol 2 (2) ◽  
pp. 195-206
Author(s):  
Komal Nawwal ◽  
Amjad Saeed ◽  
Tanveer Hussain ◽  
Sana Ajmal ◽  
Anum Choudry ◽  
...  

Abstract: The plants Cassia fistula and Terminalia arjuna are medicinally important and produce leaves and colourful blossoms that belong to the Caesalpinioideae of the legume family and the family of Combretaceae. The antioxidant, hydrogen peroxide, and phytochemical assessment (qualitative and quantitative) of methanolic and ethanolic extracts of Cassia fistula and Terminalia arjuna flowers and leaves were investigated in this work. In qualitative phytochemical evaluation, the presence of ten different chemicals of varying degrees and classes was observed, and alkaloids, steroids, tannis, glycosides, saponins, flavonoids, and terpinoids were confirmed during qualitative screening. During quantitative analysis, the Cassia fistula L. leaves showed the highest TFC (32.783A ± 0.073) and the TPC was observed highest in Terminalia arjuna flowers (42.800A ± 0.028). The highest DPPH percentage was recorded in C. fistula flowers (21.825A ± 0.069) compared to its lowest value observed in C. fistula leaves (10.660B ± 0.053). The highest hydrogen peroxide (H2O2) scavenging activity among both plants was found in C. fistula (12.526A ± 0.146) and the lowest in its leaves (7.1470B ± 0.045). The methanolic extracts showed promising antioxidant activity. The presence of bioactive components in the leaves and flowers of Terminalia arjuna and Cassia fistula suggests that these flowers and leaves might be used as a phytochemical source and are also effective and safe as natural remedies. The biochemical analysis proved that the extracts of Cassia fistula and Terminalia arjuna plants have glycosides, flavonoids, and alkaloids that play a role in antioxidant activity.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Khairul Alam Sobuj ◽  
Md. Ariful Islam ◽  
Md. Shoebul Islam ◽  
Md. Mohidul Islam ◽  
Yahia Mahmud ◽  
...  

AbstractSeaweeds are now recognized as a treasure of bioactive compounds. However, the bioactivity of seaweed originating in Bangladesh is still unexplored. So, this study was designed to explore the secondary metabolites and antioxidant activities of solvent extracts of Padina tetrastromatica and Gracilaria tenuistipitata. Phytochemical screening and FTIR spectra confirm the diverse type of bioactive compounds. Antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power (RP), phosphomolybdenum, hydrogen peroxide and nitric oxide (NO) scavenging assays. Here, methanolic extract of P. tetrastromatica showed highest amount of total phenolic content (85.61 mg of GA/g), total flavonoid content (41.77 mg of quercetin/g), DPPH (77.07%), ABTS (77.65%), RP (53.24 mg AAE/g), phosphomolybdenum (31.58 mg AAE/g), hydrogen peroxide (67.89%) and NO (70.64%) assays compared to its methanolic extracts of G. tenuistipitata. This study concluded that methanol as a solvent extract of brown seaweed (P. tetrastromatica) exhibited bioactivity and antioxidant potentiality which will be useful for pharmacological as well as in functional food application.


Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 212
Author(s):  
Yeimi Cecilia Vega-Ruiz ◽  
Corina Hayano-Kanashiro ◽  
Nohemí Gámez-Meza ◽  
Luis Angel Medina-Juárez

Jatropha species have been shown to be an important source of secondary metabolites with different biological effects. Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg are distributed in the Northwestern region of Mexico, are adapted to extreme weather conditions and are widely used (stems, leaves, and sap) in traditional medicine. The aim of the present study was to carry out the phytochemical characterization and the evaluation of the antioxidant activity in methanolic extracts of stems and leaves from J. cinerea and J. cordata. The compounds present in the extracts of both species were characterized by ESI-IT-MS/MS and quantified by HPLC-DAD. The results showed that the stem extracts of both species are rich in phenolic acids, while the leaf extracts are rich in flavonoids. Some of the main compounds found were gallic acid, gentisic acid, 3,4-Dihydroxybenzoic acid, vitexin, isovitexin, and catechol. Both species showed high concentrations of phenols and total flavonoids and antioxidant activity. J. cordata showed the highest antioxidant capacity and the highest concentration of phenolic compounds. Overall, both Jatropha species are a natural source of antioxidant compounds with potential biotechnological uses.


AGROFOR ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Amina BESSEDIK

Cuminum cyminum (in arabic: Kemoun) is an important source of therapeutic, cosmetic, bio-food and technological agents. Ancient civilizations used this plant for therapeutic purposes. Extracts from this plant are nowadays strongly used in the industrial and research fields, particularly for the medicinal, pharmacological and cosmetological purposes. Bioactive molecules extracted from Cuminum cyminum may express biocidal activities and prove to be good candidates for new antioxidants. The objective of the present work is to evaluate the biological properties of this plant, including antibacterial and antioxidant effects. The seeds of cumin are harvested at the wilaya of Biskra, and stored in a dry place until their use. They were identified at Hassiba Benbouali University of Chlef. Essential oil is obtained by hydro-distillation using a Clivenger type device (AFNOR, 2000). The obtained sample is stored in sealed bottles at low temperature (4°C) and away from light. The antibacterial effect is assessed by the disc method. The minimum inhibitory concentration (MIC) is determined by standard methods. The antioxidant activity is evaluated by the DPPH free radical trapping method of the methanolic extracts. The essential oil of C. cyminum shows a better activity against Gram+ strains compared to Gram- strains. The determination of MICs leads to the conclusion that its activity can be triggered at a very low concentration. The reaction with DPPH gives an interesting IC50. This testifies to the ability of this essential oil to reduce free radicals. Thanks to the antibacterial activities that we have highlighted, cumin can be considered as a palliative that could replace certain antibiotics. Moreover, its antioxidant activity allows us to use it as a natural food additive.


2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Gábor Janicsák ◽  
István Zupkó ◽  
Imre Máthé ◽  
Judit Hohmann

The lipid peroxidation-inhibiting activities of aqueous methanolic extracts of eleven Salvia species (Fam. Lamiaceae) were evaluated in an enzyme-independent biological system. The total polyphenol contents and the amounts of the most abundant phenoloids of the genus, caffeic and rosmarinic acids, were also determined. The EC50 values of the extracts displayed substantial differences. All of the investigated species except S. jurisicii (EC50 191.2 μg/mL) exhibited higher activities than that of ascorbic acid (EC50 123.8 μg/mL), the reference compound. Among the studied species, S. scabiosifolia (EC50 5.4 μg/mL) demonstrated the highest effect, followed in sequence by S. dumetorum, S. transsylvanica, S. officinalis l albiflora, S. nemorosa l albiflora and S. recognita (EC50 6.5 – 10.2 μg/mL). The close correlation was confirmed between the antioxidant activities and the total phenol contents of the extracts. For caffeic and rosmarinic acids, the correlation was much weaker, indicating the important role of other polyphenols in the antioxidant activity.


2018 ◽  
Vol 16 (1) ◽  
pp. 637-646 ◽  
Author(s):  
Yuehua Wang ◽  
Xu Xie ◽  
Xulong Ran ◽  
Shurui Chou ◽  
Xinyao Jiao ◽  
...  

AbstractThe polyphenol profile, antioxidant activity (particularly peroxyl radical-scavenging capacity (PSC) and cellular antioxidant activity (CAA)) and cytotoxicity of extracts from seven varieties of blue honeysuckle were compared in this study. Moreover, an analysis of correlations between individual polyphenol profiles and antioxidant activities was also conducted. Seventeen components were found in the investigated blue honeysuckle extracts, with anthocyanins being the prominent bioactive components among polyphenols. Fruit peel contained higher concentrations of individual anthocyanins compared to the fruit pulp. Beilei blue honeysuckle had the highest antioxidant activity. Correlation analysis showed that antioxidant activity values were highly associated with cyanidin-3-glucoside levels. Blue honeysuckle extracts were not cytotoxic over the range of doses tested. Among the varieties analyzed, Beilei possessed the highest antioxidant properties and was found to be the most appropriate source of natural antioxidants.


2013 ◽  
Vol 49 (4) ◽  
pp. 763-773 ◽  
Author(s):  
Barkat Ali Khan ◽  
Naveed Akhtar ◽  
Haroon Khan ◽  
Valdir de Andrade Braga

The objective of this study was to develop a pharmaceutical O/W emulsion containing plant-derived polyphenol extracts and evaluate its stability and antioxidant activity. O/W emulsions were prepared using ionic surfactant polysorbate 80 (Tween 80®). The odorwas adjusted with few drops of blue sea fragrance. DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was used to evaluate the antioxidant activity of the plant extracts alone and emulsions containing these extracts. Physical stability was assessed by submitting the emulsions to storage at 8 ºC, 25 ºC, 40 ºC and 40 ºC + 70% RH (relative humidity) for two months. Various physical characteristics of emulsions monitored, include color, creaming, liquefaction, centrifugation and pH. Brookfield rotational rheometer was used to determined viscosities and rheological behavior of emulsions. Different types of emulsion were determined microscopically, while pH values of emulsions were measured by a pH meter. Electrical conductivity data confirmed that the outer phase was water. Samples presented an acceptable pH value for an external topical use. Shear thinning behaviour was observed for all emulsions. The polyphenol-rich-plant-derived extracts alone and the extract containing emulsions showed good antioxidant activities. This research confirmed that the method used was suitable for preparing emulsions with Hippophae rhamnoids and Cassia fistula extracts, suggesting that those emulsions are suitable for topical use.


Author(s):  
Bindhu R. Kamath ◽  
Sabeena Kizhedath

Background: The use of medicinal plants for maintaining good health is getting attention worldwide. Antioxidants play an important role to protect damage caused by oxidative stress. In the present study methanolic extracts of Cassia fistula  was determined using DPPH for its antioxidant activity. Phytochemical investigation confirmed the presence of bioactive ingredients in the extract.Methods: The antioxidant activity of methanolic extract of Cassia fistula was evaluated using DPPH free radical assay. DPPH (1,1-diphenyl-2-picrylhydrazyine) free radical analysis is one of the accurate and frequently employed method for evaluating antioxidant activity.Results: The methanolic extracts showed increase in radical scavenging activity as concentration increases. The IC50 values were calculated for the methanolic extract. Ascorbic acid was used as control. Cassia fistula exhibited IC 50 of 79.42µg/ml.Conclusions: Scientific evidence suggests that antioxidants reduce the risk for chronic diseases including cancer and heart disease and infectious diseases. Further evaluation of pharmacological activities of Cassia fistula may prove useful in treatment of cancer and heart diseases.


2018 ◽  
Vol 15 (3) ◽  
pp. 699-709
Author(s):  
Vinothkanna Annadurai ◽  
Sekar Soundarapandian

Ayurveda contains a variety of medicines including the polyherbal fermented traditional medicines namely Arishta and Asava. It remains as a rich source of bioactive phytochemicals including antioxidants. There are scanty publications on the antioxidant activity of these medicines. There is a need to explore the natural antioxidants to replace synthetic counterparts which requires an assessment in terms of antioxidant activity by in vitro analysis. This study employs a range of antioxidant assay systems containing various free radicals (DPPH, hydroxyl, hydrogen peroxide, super oxide, nitric oxide and ABTS), Inhibition of free radical generation (metal chelating) and H+ – donating ability (reducing power) were performed. Ashokarishta, Aswagandharishta and Dasamoolarishta exhibited higher degree of antioxidant activities but less than the corresponding standards. Based on IC50 values, the hydrogen peroxide scavenging ability of Ashokarishta is comparable to the standard L-ascorbic acid. In ABTS assay, Aswagandharishta and Dasamoolarishta are comparable to the standard Butylated hydroxytoluene (BHT). These may be due to the phytochemical heterogeneity of the samples assayed. Thus study can lead to the sourcing of Ayuvedic polyherbal fermented medicines as a novel source of natural antioxidant molecules that might be acting as free radical quencher or scavenger and to treat human ailments.


2020 ◽  
Vol 16 (5) ◽  
pp. 581-587
Author(s):  
Pooran Golkar ◽  
Ahmad Fotoohi ◽  
Claudio Frezza

Background: Alyssum L. is a genus of herbaceous perennial or annual plants belonging to the Brassicaceae family. Little is known about biochemical properties of Alyssum species, specially endemics to Iran. In particular, the species were A. homolocarpum (Fisch. & C.A.Mey.) Boiss., A. lepidotum Boiss., A. maritimum (L.) Lam. (now syn. of Lobularia maritima (L.) Desv.) and A. simplex Rudolph. and eight accessions were studied. These diverse species accessions were collected in six different areas. Materials and Methods: In this work, the Total Phenolic Content (TPC), the Total Flavonoid Content (TFD), the Total Flavonol Content (TFL), the Carotenoid content (Car) and the Anthocyanin content (Ant) of the leaf methanolic extracts of four Alyssum L. species collected in Iran, were assessed. The antioxidant activity assay for every extract obtained from the eight accessions was also carried out according to three distinct methodologies including three different methods including: DPPH, Beta carotene/ linoleic acid and phosphomolibdate assays. Results: The highest TPC, TFD and TFL values were separately observed in two different accessions of A. lepidotum whereas the highest carotenoid content was observed in one accession of A. homolocarpum and the highest anthocyanin content was observed in A. maritimum. Different results were observed for different methodolies for antioxidant evaluations methods and some of them were found to have values, expressed in mg/mL, much lower with respect to the control. The least DPPH activity and the highest total antioxidant activity with phosphomolybdate assay was found in A. lepidotum. Conclusion: The preliminary phytochemical screening and the evaluation of their antioxidant activities were reported here for the first time for Iranian Alyssum species. The findings eventually recommends, the use of those accessions in the ethnopharmacological and nutraceutical fields.


2019 ◽  
Vol 37 (2) ◽  
pp. 71-84
Author(s):  
M. D. Ayoola ◽  
A. C. Adebajo ◽  
E. M. Obuotor ◽  
T. O. Oladapo ◽  
T. C. Fleischer

Leaves of Olax subscorpioidea, Hoslundia opposita, Cleistopholis patens, Plumbago zeylanica and Dioscoreophyllum cumminsii that are used as anti-diabetics were evaluated for hyperglycaemic-lowering and antioxidant activities to justify their Nigerian ethnomedicinal usage. Leaf methanolic extracts (100, 200 and 400 mg/kg, p.o.) were assayed in normal, glucose- and alloxan-induced diabetic rats, while 1,1,-diphenyl-2-picrylhydrazyl, total antioxidant capacity, ferric reducing antioxidant power and hydroxyl radical scavenging tests were used for their antioxidant activity. Effects of n-hexane, dichloromethane, ethylacetate and aqueous partition fractions of the three most active anti-hyperglycaemic extracts were also tested in glucose-loaded rats. In normoglycaemic rats, all extracts generally lacked activity, while with glucose-loaded rats, only O. subscorpioidea (200mg/kg) and C. patens (400 mg/kg) at 1 h and O. subscorpioidea (200, 400 mg/kg) and C. patens (400 mg/kg) at 2-4 hours gave lowered (p < 0.05) blood glucose levels than glibenclamide (5 mg/kg), while activity of H. opposita and P. zeylanica (200 mg/kg) were only comparable to glibenclamide. When daily fed for fourteen days to alloxan-induced diabetic rats, all extracts, at their most active doses, gave significantly higher activity than glibenclamide. Olax subscorpioidea leaf extract had the highest hyperglycaemic-lowering and least antioxidant activities. Highest antioxidant activity of H. opposita may suggest some contribution of antioxidant property to its hyperglycaemic-lowering activity. The glucose-lowering and insulinotropic constituents of O. subscorpioidea, H. opposita and C. patens should be concentrated in their aqueous, aqueous and dichloromethane fractions, respectively. Anti-hyperglycaemic ethnomedicinal use of these plants was justified and H. opposita has additional antioxidant property. Keywords: Antioxidant activity; Diabetes mellitus; Nigerian anti-diabetic plants 


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