In vitro Antioxidant activity and Phytochemical composition of Syringodium isoetifolium

2021 ◽  
pp. 6201-6206
Author(s):  
Kalaivani P. ◽  
Kavitha D. ◽  
Amudha P.
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
High Concentration ◽  
Hydroalcoholic Extract ◽  
Therapeutic Uses ◽  
Syringodium Isoetifolium ◽  
Shallow Estuaries ◽  
In Vitro Antioxidant Activity

Seagrass are the marine flowering plants found mainly in clear, shallow estuaries and coastal waters. In all temperate and trophical region seagrasses grow both internally and subtidally. One such seagrass namely Syringodium isoetifolium has many medicinal properities. This seagrass have most promising pharmacological activities which may include anti-inflammatory, anticancer, antidiarrheal, antihaemorrhoidal activities. This study is focussed on the phytochemical evaluation and in vitro antioxidant activity of aqueous, ethanol and hydroalcoholic extract of Syringodium isoetifolium. The qualitative analysis of Syringodium isoetifolium shows the presence of tannin, saponin, flavonoids, steroids, terpenoids, alkaloids, anthraquinone, polyphenol and coumarin. In all the three extracts only ethanol shows the high concentration of phytocompounds. Emodins, glycoside and anthocyanin were found to be absent in all the three extracts. Quantitative analysis of total phenol, flavonoid, saponin and tannin were found to be 193.10 ±13.52, 106.11 ± 7.42, 52.96 ± 3.64 and 81.30 ± 5.69. Superoxide anion radical, Nitric oxide and Hydroxy radical scavenging assay showed that Syringodium isoetifolium was an excellent scavenger of these radicals. These results are an indication of the potent antioxidant property of the extract and may be responsible for some of the therapeutic uses of Syringodium isoetifolium.

scholarly journals Phytopharmacological and Antioxidant Analysis of Hydroethanolic Extract of Boerhavia diffusa

2020 ◽  
Vol 11 (1) ◽  
pp. 840-846
Author(s):  
Murugan K ◽  
Kalaivani P ◽  
Vanitha V ◽  
Bothiraj K.V.
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Chemical Test ◽  
Therapeutic Uses ◽  
Total Antioxidant ◽  
Hydroethanolic Extract ◽  
Boerhavia Diffusa

Boerhavia diffusa, a well-known ethno-medicinal plant and were found in tropics and sub-tropics. Boerhavia diffusa is a traditional medicinal plant and they can cure many human ailments. It has most promising pharmacological activities include antifibrinolytic, immunosuppressive, diuretic, hepatoprotective, antidiabetic, anti-inflammatory, anticancer, immunomodulatory, antilympho proliferative, analgesic properties and also for the treatment of pulmonary tuberculosis. Due to their adverse side effects, the naturally occurring antioxidants has increased and replaced by the synthetic antioxidants. This study is focussed on the phytochemical evaluation and in vitro antioxidant activity of Hydroethanolic extract of Boerhavia diffusa. The qualitative chemical test exhibited the presence of phytocompounds like flavonoids, polyphenols, anthocyanins, coumarin, steroids, terpenoids, triterpenoids, glycosides, emodins and saponins. Quantitative analysis of total phenol, total flavonoid and total tannin was found to be 205.25mg/g, 121.65 mg/g and 61.41mg/g. DPPH, Total antioxidant activity, Superoxide anion radical, Nitric oxide and Hydroxy radical scavenging assay showed that B.diffusa was an excellent scavenger of these radicals. These results are an indication of the potent antioxidant property of the extract and may be responsible for some of the therapeutic uses of Boerhavia diffusa.

ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (10) ◽  
pp. 34-41
Author(s):  
Kanthlal S. K. ◽  
Jipnomon Joseph ◽  
Bindhu P. Paul ◽  
Vijayakumar M ◽  
Rema Shree A. B. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Bioactive Constituents ◽  
Preliminary Assessment ◽  
Reducing Power Assay ◽  
Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

scholarly journals Antioxidant and Hypoglycemic Properties of Seeds of Cucurbitaceae Family from Western Nepal

2019 ◽  
Vol 9 (2) ◽  
pp. 1-5
Author(s):  
Atisammodavardhana Kaundinnyayana ◽  
Anita G.C ◽  
Asmita Banstola ◽  
Kishwor Poudel ◽  
Nirmal Acharya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Lagenaria Siceraria ◽  
Benincasa Hispida ◽  
Ic50 Values ◽  
In Vitro Antioxidant Activity

Introduction: Diabetes mellitus is an endocrine and metabolic disorder characterized by the defect in insulin secretion or insulin action or both. The association between diabetes mellitus and oxidative stress is well established. Cucurbitaceae is the largest family containing 120 genera mostly grown for its sweet and juicy fruit in warm climates all over the world. In the present work ethanolic extracts of the seeds of Trichosanthes cucumerina Linn (Chichinno), Lagenaria siceraria (Lauka), Cucurbita pepo (Pharsi), Luffa aegiptiaca (Ghiraula), and Benincasa hispida (Kubindo) collected from Western Nepal has been evaluated for their in vitro antioxidant activity and in vivo hypoglycemic effects. Methods: In vitro antioxidant activity was assessed by using DPPH free radical scavenging activities and their IC50 values were calculated. In vivo hypoglycemic effect was examined on normoglycemic rats. The clinical significance of ethanolic extract at the doses of 250 and 500 mg/kg body weight was investigated in 0, 30, 60, 120 and 180 minutes of oral administration. Metformin treated group was used as the positive control. Results: For DPPH radical scavenging action, the IC50 values of Trichosanthes cucumerina, Luffa aegiptiaca, Benincasa  hispida, Cucurbita pepo, Lagenaria siceraria were found to be 60.72, 127.73, 49.63, 98.16 and 52.46 µg/ml respectively which were compared to the IC50 value of ascorbic acid (Positive control) which was found to be 38.11 µg/ml. The extracts of Benincasa hispida, and were having higher antioxidant activity and tested for in vivo hypoglycemic activity.In vivo administration of two doses of ethanolic extract of Lagenaria siceraria reduced the level of blood glucose while the best result was obtained at 250 mg/kg. Conclusions: Present study revealed promising antioxidant and hypoglycemic activity of ethanolic extract of Lagenaria siceraria. The further exploration of Lagenaria siceraria for its effective use in the traditional medicinal system is essential.

scholarly journals Chromatographic and Spectroscopic Fingerprinting of Ficus carica and Evaluation of In Vitro Antioxidant Activity

2021 ◽  
Vol 25 (03) ◽  
pp. 677-682
Author(s):  
Amara Javaid
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Ficus Carica ◽  
In Vitro Antioxidant Activity

The present study was conducted to evaluate the in vitro antioxidant activity of Ficus carica, commonly known as fig. Methanol and ethanol extracts of F. carica leaves were subjected to 2, 2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity assay where ascorbic acid being positive control had an IC50 value of 3.98±0.26 while methanol and ethanol fractions showed an IC50 of 101.76±1.12 and 93.12±1.17 respectively exhibiting their high antioxidant potential. DPPH assay was also performed on high performance liquid chromatography (HPLC) elutions. Most active antioxidant components in ethanol extract were eluted between 17–18 min, and those in methanol were eluted over 14–15 min and upon ultra-high performance liquid chromatography-mass spectrometery (Orbitrap Liquid Chromatography-Mass Spectrometry) were identified to be 13-Docosenamide, (Z)- for ethanol and ficusin for methanol fraction. Thus, it is concluded that these two components are most probable determinants of antioxidant potential of F. carica leaf extracts. © 2021 Friends Science Publishers

scholarly journals In-vitro Antioxidant Activity and Antimicrobial Activity of Hydroalcoholic Extracts of Vernonia cinerea

2019 ◽  
Vol 9 (1) ◽  
pp. 225-228
Author(s):  
Jaswinder Mehta ◽  
Peenu Mahendra Joshi ◽  
Priyanka Kushwaha ◽  
Geeta Parkhe
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Pathogenic Bacteria ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Vernonia Cinerea

The aim of present study was to estimate the in vitro antioxidant potential and antimicrobial activity of hydroalcoholic extract of Vernonia cinerea. Antioxidant activity was assessed by using 2, 2- diphenyl-1-picryl-hydrazyl (DPPH) assay using ascorbic acid as standard antioxidant. The extract was found to scavenge effectively the free radicals. The total flavonoid contents were determined by established methods and were found to be 0.547 mg/100mg in quercitin equivalents. Antimicrobial activity was performed against 2 stains of human pathogenic bacteria by well diffusion method. Hydroalcoholic extract of Vernonia cinerea showed good antimicrobial activity against gram positive bacteria. The antioxidant activities may be attributed to the presence of significant amounts of flavonoid compounds. Results indicated that hydroalcoholic extract of Vernonia cinerea possess significant antioxidant effect in dose dependent manner, followed by the hydroalcoholic extract of Vernonia cinerea possessed good antimicrobial activity. Keywords: Antioxidant activity, Radical scavenging activity, Free radicals, Antimicrobial activity.

scholarly journals Green Synthesis, Characterization and In-vitro Antioxidant Property of Silver Nanoparticles Using the Aqueous Leaf Extract of Justicia carnea

2021 ◽  
pp. 20-30
Author(s):  
I. O. Salaudeen ◽  
M. O. Olajuwon ◽  
A. B. Ajala ◽  
T. O. Abdulkareem ◽  
S. A. Adeniyi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Silver Ions ◽  
Antioxidant Property ◽  
Biological Macromolecules ◽  
Aqueous Leaf Extract ◽  
In Vitro Antioxidant Activity

This study investigated the synthesis, characterization and in vitro antioxidant activity of silver nanoparticles (AgNPs) using the aqueous leaf extract of Justicia carnea.  The aqueous leaf extract of J. carnea was used as a potential reducing and capping agent. To identify the compounds responsible for the reduction of silver ions, the functional groups present in the plant extract were subjected to FTIR. The in vitro antioxidant activity of synthesized nanoparticles was evaluated in terms of ferric reducing antioxidant potential (FRAP), DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2`-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) free radicals scavenging assays. The surface plasmon resonance confirmed the formation of AgNPs with maximum absorbance at kmax = 446 nm. FTIR revealed the biological macromolecules of J. carnea leaf extract involved in the synthesis and stabilization of AgNPs. UV-Visible spectrophotometer showed absorbance peak in the range of 436-446 nm. The silver nanoparticles exhibited moderate antioxidant activities compared to standard antioxidants (ascorbic acid and BHT). These results confirmed this protocol as simple, eco-friendly, nontoxic and an alternative for conventional physical and chemical methods. It can be concluded that J. carnea leaf extract can be used effectively in the production of potential antioxidant AgNPs which could be useful in various bio-applications such as cosmetics, food and biomedical industry.

scholarly journals HPLC profiling of selected phenolic acids and flavonoids in Salvia eigii, Salvia hierosolymitana and Salvia viridis growing wild in Jordan and their in vitro antioxidant activity

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9769
Author(s):  
Hala I. Al-Jaber ◽  
Ashok K. Shakya ◽  
Zaha A. Elagbar
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Salvianolic Acid B ◽  
Total Flavonoids ◽  
High Concentration ◽  
Total Flavonoids Content ◽  
Concentration Levels ◽  
In Vitro Antioxidant Activity

Background Salvia eigii., Salvia hierosolymitana and Salvia viridis are native to the Mediterranean region, and are used in traditional medicine for the treatment of many ailments. In the current investigation, the methanolic extracts obtained from the air dried aerial parts of S. eigii, S. hierosolymitana and S. viridis from Jordan were screened for their total phenolics content (TPC), total flavonoids content (TFC) and their in vitro antioxidant activity. Additionally, the presence of four bioactive phenolic acids including gallic acid, caffeic acid, rosmarinic acid and salvianolic acid B and other seven flavonoids including luteolin-7-O-glucoside, apigenin, apigenin-7-O-glucoside, rutin, nariginin, hesperidin and quercetin was determined using Liquid chromatography-Electron Spray Ionization-Tandom Mass Spectrometry (LC-ESI-MS/MS). Methods Antioxidant activity of the obtained three extracts were examined via the DPPH•, ABTS• + radical scavenging methods in addition to Ferrous Ion Chelating (FIC) effect. TFC and TPC of the extracts were measured using the aluminum chloride colorimetric method and the Folin-Ciocalteau method, respectively. The presence and concentration of the selected 11 compounds was further determined through LC-ESI-MS/MS. Results The results indicated that three Salvia species had high total flavonoids content expressed in mg quercetin/g dry extract (S. heirosolymitana: 770.85 ±  5.26; S. eigii: 520.60 ±  6.24, S. viridis: 311.36 ±  4.41). S. heirosolymitana had the highest DPPH• activity (0.184 ±  1.22 × 10−2 mg/ml) and FIC effect (0.354 ±  0.018 mg/ml). S. heirosolymitana had slightly higher ABTS• + scavenging activity than S. eigii (0.176 ±  1.16 × 10−2 mg/ml; 0.183 ±  0.031 mg/ml, respectively). All 11 compounds were detected in the extracts of the three Salvia species. Luteolin-7-O-glucoside was detected in high concentration levels in the three species (1756.73, 21651.36, and 26125.14 mg/kg dry plant; S. eigii, S. hierosolyimitana and S. viridis, respectively), yet rosmarinic acid had the highest contribution to both S. hierosolymitana (27124.93 mg/kg) and S. eigii (15783.33 mg/kg). Notably, S. hierosolymitana and S. viridis contained salvianolic acid B (896.11; 890.9 mg/kg). Conclusions The three Salvia species exhibited good antioxidant activity, especially S. heirosolymitana due to its high TPC, TFC, and the presence of high concentration levels of romarinic acid and other phenolic acids and flavonoids. This is the first phytochemical and antioxidant evaluation of S. eigii, S. hierosolymitana and S. viridis from Jordan. Prior to this investigation, no phytochemical investigation on S. eigii was reported.

scholarly journals Evaluation of preliminary phytochemical screening, acute toxicity and antioxidant profile of Ocimum kilimandscharicum

2019 ◽  
Vol 9 (2) ◽  
pp. 372-375 ◽  
Author(s):  
Gaurav Saxena ◽  
Abhilasha Mittal ◽  
Abdul Wadood Siddiqui
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Sprague Dawley ◽  
Oral Toxicity ◽  
Clinical Safety ◽  
Free Radical Activity ◽  
In Vitro Antioxidant Activity

Nature has offered us diverse curative herbs having with powerful antioxidant phytochemicals. Ocimum (Lamiaceae) is a notable source of volatile oils and flavouring agents in general and primarily of eugenol, methyl eugenol, linalool, methyl chavicol, etc. Karpoora Thulasi is a member of this genus; nevertheless, not much literature has been reported on its safety and antioxidant potential. In this investigation, we did a pre-clinical safety assessment of concentrate of O. kilimandscharicum on Sprague Dawley rodents. Toxicological concordat of the O. kilimandscharicum concentrate was carried out following OECD guidelines 423. Further, to verify the traditional efficacy and elucidate the mechanism, the present study compared in-vitro antioxidant activity of the plant by DPPH, ABTS and Hydroxyl radical scavenging method using ascorbic acid as the standard. In acute oral toxicity, no treatment-related death or toxic signs were observed. Moreover, the study revealed that the O. kilimandscharicum extract could be well tolerated up to the dose 2000 mg/kg body weight and could be classified as Category 5. Moreover, ABTS free radical activity of the extract was 79.2148 while that of DDPH Inhibition potential was found to be 70.72758. Our findings present substantiation that the crude extracts of O. kilimandscharicum is a likely source of natural antioxidants, and this justified its long-established uses.  Keywords: Ocimum kilimandscharicum, Acute Toxicity, In-vitro Antioxidant Activity DPPH, ABTS

scholarly journals Antioxidant Activity of Plant Extracts from Colombian Coffee-Growing Eco-Region

2017 ◽  
Vol 13 (1) ◽  
pp. 56-59
Author(s):  
Aura M. Blandón ◽  
Oscar M. Mosquera ◽  
Antônio E. G. Sant’ana ◽  
Aldenir F. Dos Santos ◽  
Luana L. S. Pires
Keyword(s):  
Antioxidant Activity ◽  
Plant Species ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Dpph Assay ◽  
Methanolic Extracts ◽  
In Vitro Antioxidant Activity

The present study describes the in vitro antioxidant activity of methanol extracts of 34 plant species collected in the Colombian coffee-growing eco-region belonging to Euphorbiaceae, Piperaceae and Solanaceae families. The antioxidant properties of extracts were evaluated by determining radical scavenging power measured with a DPPH assay. The methanolic extracts of  Hyeronimia antioquiensis, Mabea montana, and Alchornea grandis species (Euphorbiaceae), presents EC50 values equal to 0.686, 12.35, and 13.01 µg/mL, respectively, showing high antioxidant potential.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND WOUND HEALING ACTIVITY OF WHEATGRASS BY 1,1- DIPHENYL, 2 PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (12) ◽  
pp. 170
Author(s):  
Veermaneni Alekhya ◽  
Thiyagarajan Deepan ◽  
Magharla Dasaratha Dhanaraju
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Wheat Plant ◽  
Wide Range ◽  
Wound Healing Activity ◽  
In Vitro Antioxidant Activity

Objective: This study was designed to evaluate in vitro antioxidant activity and wound healing activity in Triticum aestivum (wheat grass).Methods: T. aestivum commonly known as Wheatgrass had a wide range of health benefits among the young grass of common wheat plant components includes chlorophyll, flavonoids, and Vitamins A, C, and E. Wheatgrass is used in Folklore medicine for treatment of skin diseases and wound healing. In our present study, petroleum ether, ethanol and aqueous extracts of T. aestivum have been evaluated for in vitro antioxidant activity and wound healing activity by 1,1- diphenyl, 2 Picrylhydrazyl radical scavenging activity, and Chick chorioallantoic method, respectively.Results: The results of both the assay showed that all the extracts of T. aestivum have significant antioxidant and wound healing activity on dose-dependent manner.Conclusion: The wheatgrass has antioxidant and wound healing activity.

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