A Flavonoid compound of Turbinaria decurrens Bory with The Potential Antioxidant and Anticancer Activity

2021 ◽  
pp. 6207-6210
Author(s):  
Fitriyanti Jumaetri Sami ◽  
Nunuk Hariani Soekamto ◽  
Tatsufumi Okino ◽  
Firdaus Firdaus ◽  
Jalifah latip
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Ethyl Acetate Extract ◽  
Flavonoid Compound ◽  
Mtt Method ◽  
South Sulawesi ◽  
Isolation And Characterization ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ft Ir

Isolation and characterization of quercetin flavonoid compound from Dutungan Island, South Sulawesi Province, Indonesia has been successfully done from ethyl acetate extract. Extraction method used maceration, isolation used chromatography, anticancer activity with MTT method and antioxidant test used DPPH radical. Structure was discussed with the FT-IR, NMR spectrophotometer and compared with the literature. Total flavonoids from ethyl acetate extract were 4.8 mgEQ/g, IC50 value of antioxidant activity was 4.23 μg/ml using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), and anticancer activity of H460 cells IC50 value was 10.95 μg/ml. The quercetin compound is potential as an anticancer and was first report in the T. decurrens Bory species.

scholarly journals Aktivitas Antioksidan Akar Bawang Merah Lokal Palu (Allium cepa Var Aggergatum L.) dengan Berbagai Kepolaran Pelarut

KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 198-205
Author(s):  
Nur Faidah ◽  
Nurhaeni ◽  
Ahmad Ridhay ◽  
Jusman ◽  
Abd. Rahman Razak ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Allium Cepa ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Root Extract ◽  
Polar Solvents ◽  
Ic50 Value ◽  
Dpph Method ◽  
Keywords Antioxidants

Research on the antioxidant activity of the local onion roots of Palu (Allium cepa Var Aggregatum L.) with various polarity solvents has been carried out. This study aims to determine the potential of Palu local shallot root extract as an antioxidant and determine the IC50 value of the Palu local shallot root antioxidant activity. The method applied in this research is maceration using n-hexane, ethyl acetate, and ethanol. The extracts from each solvent were tested for their antioxidant activity by the DPPH method. The results of the study showed that the IC50 value in n-hexane extract, ethyl acetate extract, and ethanol extract were 579.98 ppm, 518.28 ppm 315.83 ppm and as a comparison used ascorbic acid obtained IC50 of 53.69 ppm. Based on the results obtained, the best antioxidant activity is in polar solvents (ethanol extract) compared to other solvents. Keywords: Antioxidants, roots, Palu local shallots, Allium cepa Var Aggregatum L.

scholarly journals Inhibitory Activity of HEp-2 Cells by Honey from Indonesia

2019 ◽  
Vol 22 (6) ◽  
pp. 317-325
Author(s):  
La Ode Sumarlin ◽  
Anna Muawanah ◽  
Farhan Riza Afandi ◽  
Adawiah Adawiah
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Laryngeal Cancer ◽  
Inhibitory Activity ◽  
Ethyl Acetate Extract ◽  
Cytotoxicity Test ◽  
Inhibition Activity ◽  
Tetrazolium Bromide ◽  
Mtt Method ◽  
Diphenyl Tetrazolium Bromide

Indonesian local honey contains active compounds that have the potential as an antioxidant and anticancer, primarily as a laryngeal anticancer through the inhibition of HEp-2 cells. This study aims to determine the anticancer activity of several types of honey in Indonesia through the inhibition of HEp-2 cells. Samples used in the form of Trigona, Longan, Rambutan, and Kaliandra honey obtained from honey farmers in Sulawesi and Java, Indonesia. Honey samples extracted by using methanol, then liquids partition was carried out consecutively using n-hexane and ethyl acetate. The cytotoxicity test for HEp-2 cells was carried out using the MTT method (3- (4,5-dimethyl thiazol-2-il) -2,5-diphenyl tetrazolium bromide). The results showed that all honey samples were active against preventing HEp-2 cells with the highest inhibition activity from longan honey with ethyl acetate extract at 65.18% at 100 ppm. Longan honey has decreased HEp-2 cell inhibitory activity after fractionation. Indonesian local honey, namely trigona honey, kaliandra honey, rambutan honey, and longan honey, can be used as a supplementary supplement for patients with laryngeal cancer.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

2021 ◽  
Vol 3 (2) ◽  
pp. 53-61
Author(s):  
Angga Crystal Loasana Yami ◽  
Irmanida Batubara ◽  
Kholis Abdurachim Audah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Ic50 Value ◽  
Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

scholarly journals Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

2018 ◽  
Vol 1 (2) ◽  
pp. 41-47
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Mardiana
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories.       Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

scholarly journals CYTOTOXIC ACTIVITY OF ERIOCAULON CINEREUM R.BR TO MCF-7 AND VERO CELL LINE

2019 ◽  
pp. 94-96
Author(s):  
ARDE TOGA NUGRAHA ◽  
ASGAR PURNAMA ◽  
SITI NURUL KOMARIAH ◽  
HADY ANSHORI T ◽  
HADY ANSHORI T
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Vero Cells ◽  
Chromatography Method ◽  
Mtt Method ◽  
Ic50 Value ◽  
Normal Human ◽  
Liquid Chromatography Method ◽  
Vacuum Liquid Chromatography ◽  
Mcf 7

Objective: The aim of the study was to determine the strength of the Eriocaulon cinereum R.Br plant against breast cancer cells (MCF7) and cytotoxicagainst Vero cells.Methods: E. cinereum R.Br was taken from the province of Bangka Belitung and then extracted in stages with n-hexane, ethyl acetate, and methanol.Then, the ethyl acetate extract was fractionated using the vacuum liquid chromatography method with dichloromethane and ethyl acetate solvents.Sample was tested for MCF-7 cells and Vero cells using the MTT method (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). The dataobtained is analyzed by probit SPSS.Results: The results of this study showed that the best IC50 extract was ethyl acetate extract with an IC50 value of 450.31 μg/ml. Then, ethyl acetateextract produced dichloromethane fraction with IC50 value of 443.52 μg/ml and ethyl acetate with IC50 value of 214.75 μg/ml. Ethyl acetate fractionwas also tested against Vero cells to see cytotoxic against normal human cells with IC50 679.11 μg/ml

scholarly journals FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BATANG Chisocheton sp. (C.DC) Harms (Meliaceae)

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Dewa Gede Katja
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Test Results ◽  
Ic50 Value ◽  
Dpph Method ◽  
Stem Bark Extract

Tujuan penelitian ini untuk mengetahui golongan senyawa metabolit sekunder dan aktivitas antioksidan dari setiap ekstrak kulit batang Chisocheton sp. C.DC Harms (Meliaceae). Hasil ekstraksi 200 g serbuk kulit batang Chisocheton sp. C.DC Harms (Meliaceae) dengan n-heksana, etil asetat dan metanol masing-masing dengan 2000 mL berturut-turut menghasilkan 7,193 g ekstrak pekat n-heksana, 8,798 g ekstrak pekat etil asetat dan 18,683 g ekstrak pekat metanol. Hasil uji fitokimia menunjukkan adanya golongan senyawa flavonoid, triterpenoid dan tanin. Hasil uji aktivitas antioksidan dengan metode DPPH menunjukkan bahwa ekstrak n-heksana memberikan nilai IC50 sebesar 337,28 µg/mL, ekstrak metanol sebesar 216,73 µg/mL, dan ekstrak etil asetat sebesar 199,89 µg/mL yang berarti etil asetat memiliki kemampuan yang paling besar dalam menangkap radikal bebas.ABSTRACTThe objective of this study was determine the class of secondary metabolite compounds and antioxidant activity of each stem bark extract of Chisocheton sp. C.DC Harms (Meliaceae). The results of the extraction of 200 g Chisocheton sp. C.DC Harms (Meliaceae) with n-hexane, ethyl acetate and methanol with 2000 mL each yielded 7,193 g of concentrated extract n-hexane, 8,798 g of concentrated extract of ethyl acetate and 18,683 g of concentrated methanol extract. Phytochemical test results showed the presence of flavonoid, triterpenoid and tannin compounds. The results of the antioxidant activity test using the DPPH method showed that the n-hexane extract gave an IC50 value of 337,28 µg/mL, methanol extract was 216,73 µg/mL, and ethyl acetate extract was 199,89 µg/mL which means that ethyl acetate has the ability to greatest in capturing free radicals.

scholarly journals Antioxidant Activity Test and Determination Of Total Flavonoids Levels of Kedondong Laut (Nothopanax fructicosum (L.) Miq) Leaf Extract

2020 ◽  
Vol 11 (2) ◽  
pp. 2493-2497
Author(s):  
Virsa Handayani ◽  
Andi Amaliah Dahlia ◽  
Nurul Hasanah ◽  
Aulia Nur Siti Nur Aisya Amin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Flavonoid Compound ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
The Family

Kedondong laut (Nothopanax fruticosum (L.) Miq.) is a plant from the family Araliaceae. Leaves of kedondong laut useful for diuretik, analgesics, as an antidiarrheal and  arthritis. The characteristics of sea kedondong are shrubs, yellowish green, the size of small flowers are green and the fruit is greenish purple and the height of the tree reaches up to 3 meters. Extracted by stratified extraction using solvents n-Hexane, ethyl acetate, and 96% ethanol with a percent rendender for n-Hexane solvent 1.504% ethyl acetate 2.784%, ethanol 3.698%. The results showed that each extract of kedondong laut leaves (Nothopanax fructicosum (L.) Miq has antioxidant activity with IC50 value of n-hexane extract of 33.839µg/mL, ethyl acetate extract of 12.604µg/mL and ethanol extract of 2.222 µg/mL ethanol extract of kedondong laut leaves (Nothopanax fructicosum (L.) Miq) has higher antioxidant power than n-hexane extract and ethyl acetate, the total flavonoid compound content is 0.09902 gQE/g extract or 9.902%, ethyl acetate extract is equal to 0.13253 gQE /g extract or 13.253%, and 96% ethanol extract at 0.09345 gQE / g extract or 9.345%, ethyl acetate extract has a greater flavonoid content than n-hexane extract and 96% ethanol.

scholarly journals ISOLASI DAN KARAKTERISASI EKSTRAK ETANOL DAUN LEILEM (Clerodendrum minahassae Teijsm dan Binn.) DENGAN METODE SPEKTROFOTOMETRI ISOLATION AND CHARACTERIZATION ETHANOL EXTRACT OF LEILEM LEAVES (Clerodendrum minahassae Teijsm and Binn) WITH METHODS SPECTROPHOTOMETRIC

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Yuri Pratiwi Utami ◽  
Imrawati Imrawati ◽  
Abd Rasyid
Keyword(s):  
Positive Result ◽  
Ethyl Acetate ◽  
Bacterial Cell ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Isolation And Characterization ◽  
Ft Ir

ISOLASI DAN KARAKTERISASI EKSTRAK ETANOL DAUN LEILEM (Clerodendrum minahassae Teijsm dan Binn.) DENGAN METODE SPEKTROFOTOMETRIISOLATION AND CHARACTERIZATION ETHANOL EXTRACT OF LEILEM LEAVES (Clerodendrum minahassae Teijsm and Binn) WITH METHODS SPECTROPHOTOMETRICYuri Pratiwi Utami1), Imrawati1), Abd.Rasyid.D1)1)Program studi sarjana farmasi, Sekolah Tinggi Ilmu Farmasi MakassarJl. Perintis Kemerdekaan KM.13,7 Daya telp/fax. 0411-583190 Makassar [email protected] leaf (Clerodendrum minahassae Teijsm .And Binn.) have been reported in traditional medicine for the treatment of various deseases such as stomatch ache and ascariasis. In Minahassa, leilem leaf generally consumed as vegetables. The present study revealed that leilem leaf can inhibit the growth of escherhia coli because of the presence of flavonoid that can demage bacterial cell membranes. This study at characterization of isolated compounds from ethanol extract of leilem leaves (Clerodendrum minahassae Teijsm and Binn). Extraction was done maceration using 70% ethanol with the percentage of rendement was 10,19%. The phytochemical analysis of ethanol extract of leilem leaves showed a positive result of alkoloid, flavonoid, streroid and tanin. The ethyl acetate extract was selected and 2 grams of it was taken to continued to second conventional column chromatography and 5 fractions were obtained. Fraction 2 was choosen and continued to preparative and result 2 isolates. Isolate 2 that showed one spot on tlc with rf 0,66. Was continued for spectrofotometri uv-vis and ftir analysis. The spectrophotometer uv-vis maximum wavalength at 269,0 nm and 333,0 nm. The ftir data indicated the presence of functional O-H, C-H, C=C and C-O which is suspected ad flavonoid.Keywords : Isolation, Characterization, leilem leaf (Clerodendrum minahassae Teijsm and Binn)ABSTRAKDaun leilem (Clerodendrum minahassae Teijsm dan Binn.) dilaporkan yaitu sebagai obat tradisional untuk menyembuhkan beberapa penyakit, seperti sakit perut dan Ascariasis. Daun leilem biasanya dikonsumsi sebagai sayuran oleh masyarakat di Minahasa. Penelitian ini bertujuan untuk mengetahui profil (karakter) senyawa yang diisolasi dari ekstrak etanol daun leilem (Clerodendrum minahassae Teijsm dan Binn.) ekstrak diperoleh dari proses maserasi menggunakan pelarut etanol 70% dengan persen rendamen 10,19%. Hasil uji kandungan kimia ekstrak etanol daun leilem positif mengandung alkaloid, flavonoid, steroid dan tanin. Ekstrak etil astetat dipilih dan diambil 2 gram dilanjutkan untuk dilakukan pemisahan menggunakan komatografi kolom konvensional dan menghasilkan 5 fraksi. Fraksi 3 dipilih dilanjutkan kromatografi kolom konvensional kedua dan didapatkan 5 fraksi. Fraksi 2 dipilih dan dilanjutkan ketahapan kromatgrafi lapis tipis preparatif dan menghasilkan 2 isolat. Isolat 2 yang menunjukkan 1 noda tunggal pada profil KLT dengan niliai Rf 0,66 dilanjutkan untuk dianalisis dengan spektofometri UV-Vis dan FT-IR. Hasil analisis spektrofotometri UV-Vis isolat 2 mempunyai serapan maksimal pada panjang gelombang 269,0 nm dan 333,0 nm-1. Data FT-IR menunjukkan adanya gugus fungsi O-H, C-H, C=C dan C-O yang diduga merupakan senyawa flavonoid. Kata kunci: Isolasi, karakteriksasi, Daun Leilem(Clerodendrum minahassae Teijsm and Binn). 

scholarly journals Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers

2016 ◽  
Vol 1 (2) ◽  
pp. 71
Author(s):  
Liza Pratiwi ◽  
Achmad Fudholi ◽  
Ronny Martien ◽  
Suwidjiyo Pramono
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Garcinia Mangostana ◽  
Ic50 Value ◽  
Dpph Method ◽  
Peel Extract

<span>Mangosteen peels (Garcinia mangostana L.) is well known as an excellent source of antioxidative compounds. The name of mangosteen is xanthone. Antioxidant of mangosteen  peels can be extracted by ethanol, etyl acetate and can be fractinated by etyl acetate and n-hexane. The aim of this research was to compare the antioxidant activity of the peel extract by ethanol and etyl acetate and fractinated by etyl acetate and n-hexane. Extract and fraction exhibited higher scavenging activity of DPPH. </span><span lang="EN">The purpose of this study was to compare antioxidant activity of ethanol extract, ethyl acetate extract, fraction of ethyl acetate and n-hexane fraction.</span><span> The</span><span lang="EN"> antioxidant activity </span><span>test </span><span lang="EN">using DPPH method with UV-Vis spectrophotometer</span><span>. Ethanol extract shown IC50 value 5,03 µg/mL, ethyl acetate extract shown IC50 value 41,56 µg/mL. Ethyl acetate fraction shown IC50 value 2,78 µg/mL, and n-hexane fraction with IC50 22,33 µg/mL. It means peel extract and fraction by mangosteen peels has very strong antioxidant activity and ethyl acetate fraction that its antioxidant activity higher that the other solvent.</span>

scholarly journals ISOLATION AND IDENTIFICATION OF FLAVONOID COMPOUND EXTRACTIRE ETHYL ACETATE FRACTION EXTRACTED FROM THE RHIZOMES FINGERROOT OF (Boesenbergia pandurata (Roxb.) Schlecht) (Zingiberaceae)

2010 ◽  
Vol 6 (2) ◽  
pp. 219-223 ◽  
Author(s):  
Ochtavia Prima Sari ◽  
Titik Taufiqurrohmah
Keyword(s):  
Liquid Chromatography ◽  
Melting Point ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Compound ◽  
Isolation And Identification ◽  
Ft Ir ◽  
Vacuum Liquid Chromatography ◽  
Isolated Compound

Boesenbergia pandurata (Roxb.) Schlecht is one of fingerroot plant in ginger family (Zingiberaceae). The rhizomes of the plant contained a lot of secondary metabolites compounds. Therefore, the purpose of the research is to isolate and identify the flavonoid compound from the plant. The rhizomes were extracted with metanol continued by partition using ethyl acetate-water (1:1). The ethyl acetate extract was chromatographed on a column of Si gel (Vacuum Liquid Chromatography and Gravitation Column Chromatography) using n-hexane-ethyl acetate (5:2) as eluents. Further purification by recristalization  using benzene  produced a compound as yellow powder (16 mg) having melting point of 294-295 oC. The spectra of isolated compound were determined by spectroscopic UV-Vis, FT-IR, and GC-MS. Spectrum UV-Vis of the isolated compound showed ultraviolet absorption at λmax (MeOH, nm) 290 and 322; λmax (MeOH+NaOH, nm) 322; λmax (MeOH+AlCl3, nm) 309; λmax (MeOH+AlCl3+HCl, nm) 310; λmax (MeOH+NaOAc, nm) 322 and λmax (MeOH+NaOAc+H3BO3, nm) 290. Its FT-IR spectrum represented a number of absorption lied on νmax (cm-1) : 3142.5; 3012.6; 2893; 2345.3; 1631.7; 1585.4; 1357.8; 1168.8; and 825.5. GC-MS spectrum of the isolated compound exhibited an [M]+ ion peak at m/z = 256 with retention time of  22,579. Based on the results of spectrum analysis it can be concluded that the compound is 5,7-dyhydroxyflavanone.   Keywords: 5,7-dyhydroxyflavanone, Boesenbergia pandurata, ethyl asetat, fingerroot

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