scholarly journals Characterization for Types and Percents of Polycyclic Aromatic Hydrocarbons in Sera of Workers at Fuels Stations at Baghdad

2021 ◽  
Vol 11 (1) ◽  
pp. 124-136
Author(s):  
Dr. Salwa Hameed Naser Al-Rubaeꞌi ◽  
Dr. Fatin Fadhel Al-Kazazz ◽  
Ali Mohammed Jawad ◽  
Mohannad Hadi Sholi ◽  
Liqaa Mouyed Mouhson ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Control Group ◽  
Total Pahs ◽  
Polycyclic Aromatic ◽  
Sera Samples

Allpolycyclic aromatic hydrocarbons (PAHs) have a hightoxicity, and/or carcinogenic and \ormutagenic to most microorganisms andhumans.Main aim to this study is to providecontemporary information (quantitative and qualitative)PAHs in sera of fuel stations, workers whom exposure with petroleum products.The study includes 60 male petrol-filling workers in 5 petrol stations around Baghdad. Theirages were between (20 – 50) years. For compared the results a 30 sera samples from healthyindividuals was collected with corresponding age-matched as control group. The samples werecollected from October 2017 until April 2018. The BMI was calculated for all subjects.Technique of High performance liquid chromatography (HPLC) was used to detect thepresence of PAHs quantitatively and qualitatively in the sera of all subjects.The mean level of total PAHs in sera of workers was 1275.65± 934.267 higher than that forhealthy subjects 67.380 ± 46.16 with significant value p=0.033.Also the types and percent ofPAHs in the sera of workers were differ.For studying the epidemiologic,no correlation betweenthe percent of % PAHs in sera of workers who exposure to petroleum withoutaffected withtheirage and BMI.

High performance liquid chromatography–tandem mass spectrometry for rapid and sensitive analysis of six polycyclic aromatic hydrocarbons in wastewater

2011 ◽  
Vol 64 (2) ◽  
pp. 477-484 ◽  
Author(s):  
Manli Wu ◽  
Huining Xu ◽  
Ya Yu ◽  
Lili Wang
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Aromatic Hydrocarbons ◽  
Mobile Phase ◽  
High Performance ◽  
Tandem Mass ◽  
Polycyclic Aromatic

A sensitive and fast method was developed to quantitatively analyse the six polycyclic aromatic hydrocarbons (fluoranthene (FLT), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), benzo[a]pyrene (Bap), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (INPY)) by high performance liquid chromatography (UPLC) coupling with tandem mass spectrometry (MS/MS). Chromatographic separation was performed on a Waters Acquity UPLCTM BEHC18 column (1.7 μm, 2.1 mm × 50 mm). A 0.2 μm precolumn filter was used to protect the analytical column. Mobile phase A was acetonitrile containing 0.5% toluene. Mobile phase B was water. Linearity of detection was in the range of 1–100 μg L−1; LOD of 5 PAHs were lower than 0.1 μg L−1; LOQ were 0.2 μg L−1 except for benzo[k]fluoranthene. The LOD and the LOQ of benzo[k]fluoranthene were respectively 0.1 μg L−1 and 0.8 μg L−1. Wastewater samples collected from two wastewater treatment plants were determined using this method respectively. Recovery of all compounds varied from 67.8 ± 10.6% to 113.2 ± 7.2%. In comparison with the existing methods, this rapid method saves time and solvent and improves instrument sample throughput by 2–5 fold.

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