Evaluation of acute and subchronic toxicity of “Tran Chau Nguu Hoang Hoan” in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 38-47
Author(s):  
Tran Thai Ha ◽  
Pham Thi Van Anh ◽  
Dao Xuan Tinh ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Toxicity Test ◽  
Acute Toxicity Test ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Microscopic Images ◽  
Renal Functions ◽  
Liver And Kidney ◽  
Oral Doses

“Tran chau nguu hoang hoan” was prepared from 12 herbal ingredients. So far, the safety of this product, has not been reported yet. Thus, this study aimed to evaluate the acute and subchronic toxicity of “Tran chau nguu hoang hoan” through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in mice at the doses of 2.42 g/kg b.w/day to 6.04 g/kg b.w/day. The subchronic toxicity was evaluated followed the Guideline of WHO and OECD in rats with oral doses of 58.0 mg/kg b.w/day and 174.0 mg/kg b.w/day for 12 consecutive weeks. As a result, in the course of the acute toxicity test, the mice showed no abnormal sign or death. In terms of the subchonic toxicity test, hematological indexes, hepato-renal functions and microscopic images of liver and kidney were unchanged. In conclusion, “Tran chau nguu hoang hoan” does not appear to produce acute and subchronic toxicities in mice and rats.

The evaluation of acute and subchronic toxicities of An Phu Khang capsules in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 86-95
Author(s):  
Ha Thi Yen ◽  
Tran Thanh Tung ◽  
Dang Thi Thu Hien
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney

The purpose of this research was to evaluate the acute and subchronic toxicities of An Phu Khang capsules through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in Swiss mice. The subchronic toxicity was evaluated by the recommendation of WHO in Wistar rats at these doses of 0.54 g/kg b.w/day (equal to recommended human dose) and 1.62 g/kg b.w/day (3 times as high as recommended human dose) in 4 consecutive weeks. As a result, An Phu Khang capsules at the highest dose used for mice (36.29 g/kg b.w) did not show acute toxicity in mice. In terms of the subchronic toxicity test, after oral administration of An Phu Khang capsules, hematological parameters, hepato-renal functions, and microscopic images of liver and kidney at both doses were unchanged compared with the control group. In conclusion, An Phu Khang with both doses 0.54 g/kg b.w/day and 1.62 g/kg b.w/day did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

The study of acute and subchronic toxicities of Da Dai Trang HVD capsules in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 7-15
Author(s):  
Pham Thi Van Anh ◽  
Nguyen Van Dam ◽  
Pham Thanh Ky ◽  
Vu Viet Hang ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Deleterious Effect ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Renal Functions ◽  
Swiss Mice ◽  
Human Dose ◽  
Oral Doses

The purpose of this research is to evaluate the acute and subchronic toxicities of DA DAI TRANG HVD capsules through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in Swiss mice. The subchronic toxicity was evaluated by the recommendation of WHO and OECD in Wistar rats with oral doses of 1.44 g/kg/day (equal to recommended human dose) and 4.32 g/kg/day (3 times as high as recommended human dose) in 4 consecutive weeks. As a result, DA DAI TRANG HVD capsules at the highest dose used for mice (99.9 g materials/kg) did not express acute toxicity in mice. In term of the subchonic toxicity test, DA DAI TRANG HVD had no deleterious effect on hematological parameters, hepato-renal functions, macroscopic and microscopic images of livers and kidneys of rats. In conclusion, DA DAI TRANG HVD capsules did not produce the acute and subchronic toxicities in Swiss mice and Wistar rats.

scholarly journals Evaluation of acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets in experimental animals

2021 ◽  
Vol 141 (5) ◽  
pp. 29-38
Author(s):  
Tran Thanh Tung ◽  
Dau Thuy Duong ◽  
Pham Thi Thuy Minh ◽  
Nguyen Thu Hien ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney ◽  
Oral Doses

The study aimed to evaluate the acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets through oral administration using experimental animal models. Acute toxicity in Swiss mice was determined using the Litchfield Wilcoxon method. The subchronic toxicity in Wistar rats was evaluated according to WHO and OECD’s recommendation with oral doses of 4.68 g/kg/day (equivalent to recommended human dose) and 14.04 g/kg/day (3 times the recommended human dose) for 4 consecutive weeks. In terms of acute toxicity, “Phuong Dong Dai Trang” tablets did not express acute toxicity in mice at the highest dose used (232.14 g materials/kg). In terms of the subchronic toxicity, after oral administration of “Phuong Dong Dai Trang” tablets, hematological parameters, hepato - renal functions, and microscopic images of liver and kidney were unchanged in the treatment group compared to the control group. In conclusion, “Phuong Dong Dai Trang” tablets did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

scholarly journals Toxicopathological Evaluation of Hydroethanol Extract ofDianthus basuticusin Wistar Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Anofi Omotayo Tom Ashafa ◽  
Mutiu Idowu Kazeem
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Acute Toxicity Test ◽  
Toxicity Profile ◽  
Subacute Toxicity ◽  
Whole Plant ◽  
Single Oral Administration ◽  
Treatment Of Diabetes

Background. Dianthus basuticusis a commonly used medicinal plant in Basotho traditional medicine for the treatment of diabetes, but there is no report on its safety or toxicity. Therefore, we evaluated the toxicity profile of the hydroethanol whole plant extract ofDianthus basuticusin Wistar rats.Methods.Acute toxicity test was performed with single oral administration of 100–3200 mg/kg body weight ofD. basuticusextract to rats and the animals were observed for 14 days for signs of toxicity. The subacute toxicity experiment was conducted by oral administration of graded doses (200, 400, and 800 mg/kg) ofD. basuticusextract daily for 28 days. Behavioural changes as well as haematological, biochemical, and histological parameters were then evaluated.Results.There was no observable sign of toxicity in the acute toxicity test. There were significant decreases (P<0.05) in the feed and water intake as well as total cholesterol and triglycerides of theD. basuticusextract-treated rats in subacute toxicity study. There were no treatment related differences in the haematological, biochemical, and histopathological evaluations.Conclusions.Administration of hydroethanol extract ofD. basuticusmay be safe at the dosages tested in this study but its continuous usage can cause anorexia.

scholarly journals Toxicological implications of the fruit of Harungana madagascariensis on wistar rats

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Olusayo Aderonke Shorinwa ◽  
Barizonmdu Monsi
Keyword(s):  
Acute Toxicity ◽  
Blood Cell ◽  
Total Protein ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Control Group ◽  
Acute Toxicity Test ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Liver And Kidney

Abstract Background The unopened buds of the fruit of Harungana madagascariensis is used in the treatment of anaemia and skin diseases in traditional medicine. Hence, this study aims to scientifically evaluate the effects of oral administration of the fruit extract of Harungana madagascariensis on haematological, biochemical and histological parameters in Wistar rats. Methods Phytochemical screening of the ethanol fruit extract of H. madagascariensis was carried out. Acute toxicity test was done using Lorke’s method. Sub-acute toxicity studies were done using 24 rats of both sexes which were randomized into four groups of six rats each. Animals in groups A, B, C were administered with the extract at doses of 250, 500 and 1000 mg/kg, respectively while group D animals were given distilled water (5 mg/kg) and served as the control group. All administrations were done through the oral route for 30 consecutive days. Body weights of the animals were taken weekly during the study. The animals were sacrificed under diethyl ether anaesthesia and blood samples collected for evaluation of haematological (red blood cell, haemoglobin, packed cell volume and white blood cell) and biochemical (alanine transferase, alanine aminotransferase, alkaline phosphatase, urea, creatinine, total cholesterol and total protein) parameters. Histological examination was conducted on the liver and kidney of the animals. Results Preliminary phytochemical screening of the extract revealed the presence of alkaloids, anthraquinones, steroidal nucleus, saponins, carbohydrates, flavonoids, and tannins. Acute toxicity test showed that the LD50 was greater than 5000 mg/kg. There was no statistically significant (P < 0.05) difference in the RBC, HB, PCV and WBC of the extract treated groups when compared to the control group. There was however, a statistically significant (P < 0.05) difference in the creatinine level of the 500 mg/kg extract –treated group and the control. There was no statistically significant (P < 0.05) difference in other biochemical parameters of the extract treated groups and the control group except for a marginal increase in the total protein in the group treated with 1000 mg/kg of the extract (60 g/L) compared with control (54.80 g/L). Histopathological examination showed alterations in the morphology of the liver and kidney in extract treated groups as compared to the control groups. Conclusion The findings have revealed that the ethanol fruit extract of H. madagascariensis should be used with caution especially during prolonged usage as the histology showed it has nephrotoxic and hepatotoxic potentials. Further studies will be done to establish the effects of the extract on white blood cells.

scholarly journals Safety Evaluation of a New Traditional Chinese Medical Formula, Ciji-Hua’ai-Baosheng II Formula, in Adult Rodent Models

2019 ◽  
Vol 2019 ◽  
pp. 1-21 ◽  
Author(s):  
Biqian Fu ◽  
Xiangyang Zhai ◽  
Shengyan Xi ◽  
Lifeng Yue ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Mortality Rate ◽  
Acute Toxicity ◽  
Toxicity Test ◽  
Recovery Period ◽  
Treated Group ◽  
Acute Toxicity Test ◽  
Rodent Models ◽  
Subchronic Toxicity ◽  
Traditional Chinese Medical

Background. Ciji-Hua’ai-Baosheng II Formula (CHB-II-F) is a new traditional Chinese medical formula that has been shown to reduce toxicity and side effects of chemotherapy and increase the probability of cancer patient survival. Whether CHB-II-F is safe as an adjunctive therapy for cancer patients receiving chemotherapy has yet to be determined. Purpose. To evaluate the acute and subchronic toxic effects of CHB-II-F in rodent models. Methods. In acute toxicity test, 24 Kunming mice were divided into 2 groups: untreated control and CHB-II-F 1.05 g/mL (31.44 g/kg) treated group. Treatment was administered to the treated group 3 times a day for 14 days. The overall health, adverse reactions, and mortality rate were documented. In subchronic toxicity test, 96 Sprague-Dawley rats were divided into 4 groups: untreated control, high dose CHB-II-F (H) (26.20 g/kg), medium dose CHB-II-F (M) (13. 10 g/kg), and low dose CHB-II-F (L) (6.55 g/kg) [equal to 24.375 g (dried medicinal herb)/kg] treated groups. Treated groups were given the treatments once a day for 4 weeks. The overall health and mortality rate were recorded every day. Body weight and food consumption were measured once a week. Hematologic and biochemical parameters, organ weights, and histopathologic markers were analyzed after 4 weeks. An additional 2 weeks were given as the treatment recovery period before end-point euthanization, and biochemical analyses were performed. Results. The maximum tolerated dose (MTD) of CHB-II-F on mice was found to be 94.31 g/kg [equal to 351 g (dried medicinal herb)/kg], which is 108 times the human adult dose. In the acute toxicity test, administration of CHB-II-F 31.44 g/kg showed no adverse effect and did not cause mortality. In the subchronic toxicity test, after 4 weeks of treatment, compared to the controls, total cholesterol (TCHO) level, cardiac and splenic indexes, body weights of female rats, and mean corpuscular hemoglobin concentration (MCHC) in the CHB-II-F (H) group were significantly increased; triglyceride (TG) in the CHB-II-F (M) group and liver and splenic indexes in the CHB-II-F (L) group were increased. After the two-week recovery period, biofluid analyses, food consumption, and histopathologic examinations showed no abnormalities. Conclusion. Administration of CHB-II-F had no obvious adverse effect on the overall health of rodent models. A daily maximum dose of less than 94.31 g/kg or 6.55 g/kg CHB-II-F for 4 continuous weeks was considered safe.

Acute Toxicity Test on Old Smelting Slag

2010 ◽  
Vol 160-162 ◽  
pp. 1564-1568
Author(s):  
Guo Hong Shui ◽  
Dong Wei Li
Keyword(s):  
Heavy Metal ◽  
Acute Toxicity ◽  
Toxicity Test ◽  
Bodyweight Gain ◽  
Test Method ◽  
Abnormal Behavior ◽  
Control Group ◽  
Acute Toxicity Test ◽  
Significant Difference ◽  
Liver And Kidney

The total amount of heavy metal in Ming and Qing dynasties smelted residue was analyzed. Also leaching solution’s acute toxicity of heavy metal in waste residue was discussed.The results showed that the residual quantity of heavy metal(zinc and plumbum) in residue was up to 6.97%. After several hundred years of lixiviation by rainwater, heavy metal (zinc and plumbum) which had released to circumstance was more than 1.71%.Heavy metal in ancient leaching has declined and Residue in Zn is only a very small part of the leaching, Pb leaching below the detection limit ,Cr, and Cd there was leachingonly a small amount .According to pre-test results, limiting test method was adopted to carry out acute toxicity test on waste residue’s leaching solution.The results of acute toxicity test showed that acute oral LD50(median lethal doses) in mice of waste residue’s leaching solution was bigger than 20ml/kg.bw. Mice in the experiment appeared no dead and no abnormal behavior. But mice significantly decreased bodyweight gain. At the end of the experiment, mice were anatomical examined for liver and kidney. No abnormal change was found. It was no significant difference compared with the control group. It showed that residual quantity of heavy metal in residue was high. Although reduce the leaching toxicity, but the acute toxicity harm still existed.

Acute and subchronic oral toxicity assessments of BTL tea in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 48-57
Author(s):  
Tran Thai Ha ◽  
Bui Viet Chung ◽  
Pham Thi Van Anh ◽  
Nguyen Thi Ngoc ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Current Trend ◽  
Premature Death ◽  
Zingiber Officinale ◽  
Control Group ◽  
Oral Toxicity ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Human Dose

Tobacco smoking remains a leading cause of preventable diseases and premature death in many countries. Many smokers want to quit smoking but are not offered the highly effective treatments available to manage tobacco dependency. There has been a current trend for researchers to find new natural ingredients that were safe and still effective in treating tobacco dependence. BTL tea was a herbal-derived product prepared from Herba Menthae, Pogos cablin (Blanco) Benth., Zingiber Officinale Rosc., Flos Chrysanthemi, Radix Glycyrrhizae, Pericarpium Citri deliciosa, and Flos Lonicera. At this time, the safety of this product has not been reported. Thus, this study aimed to evaluate BTL tea’s acute and subchronic toxicity through oral administration in experimental animals. The acute toxicity was determined by Litchfield-Wilcoxon method in mice at the doses of 45.0 g/kg b.w/day to 112.5 g/kg b.w/day. The subchronic toxicity was evaluated following WHO and OECD’s Guidelines in rats with oral doses of 1.08 g/kg b.w/day (equal to recommended human dose) and 3.24 g/kg b.w/day (three times as high as recommended human dose) for four consecutive weeks. As a result, in the acute toxicity test, the mice showed no abnormal sign or death. The subchronic toxicity test, hematological indexes, hepato-renal functions, and microscopic images of liver and kidney were unchanged. However, compared with the control group, there were significant differences in various indexes, including total WBC, lymphocytes, neutrophils, and AST level, but the levels were still safe. In conclusion, BTL tea does not appear to produce acute and subchronic toxicities in mice and rats.

scholarly journals Acute Toxicity Test of Soursop Leaves (Annona muricata) on Liver and Kidney of Switzerland Mice

Sains Medika ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 48 ◽  
Author(s):  
Astika Widy Utomo ◽  
Neni Susilaningsih ◽  
Desy Armalina
Keyword(s):  
Single Dose ◽  
Acute Toxicity ◽  
Toxicity Test ◽  
Control Group ◽  
Acute Toxicity Test ◽  
Acute Oral Toxicity ◽  
Annona Muricata ◽  
Oral Toxicity ◽  
Group I ◽  
Liver And Kidney

Introduction: The soursoup leaves extract (Annona muricata) has widely been used as traditional medicine for cancer. No studies have been conduct to investigate the safety of the extract. Objectives: The purpose of the study was to investigate the acute oral toxicity test of soursoup leaves extract (Annona muricata) on Swiss mice’s liver and kidney.Methods: Twenty four mice were divided into 4 groups. Group I was control group, while group II-IV was given soursoup leaves extract as single dose orally via sonde. The mice were obsereved until day 7 to determine the LD50 and at the end were terminated to collect the liver and kidney. The organs later were made into histopathology slides. The slides read with light microscope. The data analyzed with ANOVA and was considered significant at p<0.05.Results: All mice were alive during the 7 days observation and no mice showing the toxic spectrum after the dosing. Microscopically, no damage on the liver and kidney organ among the groups.Conclusion: The LD50 of soursoup leaves extract is more than 2000 mg/kgBW. This result indicate that the extract is practically non toxic and do not damage the liver and kidney.

scholarly journals Acute Toxicity Study of the Combination of Azadirachta indica A. Juss. and Gynura procumbens (Lour.) Merr. Leave Extracts

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Nofran Putra Pratama
Keyword(s):  
Acute Toxicity ◽  
Azadirachta Indica ◽  
Toxicity Test ◽  
Ethanol Extract ◽  
Toxicity Tests ◽  
Public Confidence ◽  
Acute Toxicity Test ◽  
Experimental Animals ◽  
Separate Treatment ◽  
Female Wistar Rats

Azadirachta indica A.Juss. and Gynura procumbens (Merr.) are medicinal herbs widely used in traditional medicine. Recent research on the combination of these two plants showed aggressive antioxidant activity. The combination results can improve insulin and beta-cell morphology and can increase insulin expression. The variety of activities performed is the basis for conducting acute toxicity tests on the ethanol extract of Azadirachta Indica A. Juss. and Gynura procumbens (Lour.) Merr. to increase public confidence in its efficacy and ensure the safety of its use. The acute toxicity test on the ethanol extract of Azadirachta Indica A. Juss. and Gynura procumbens (Lour.) Merr. was carried out on female Wistar rats by following the 423 procedures of OECD (Organization for Economic Cooperation and Development) Guideline with five groups of experimental animals, namely normal treatment, aquadest solvent treatment and 0.5 Na-CMC 0.5%, a separate treatment of the ethanol extract of Azadirachta Indica A. Juss., a separate treatment of the ethanol extract of Gynura procumbens (Lour.) Merr., and combination treatment of the ethanol extract of Azadirachta Indica A. Juss. and leaves of Gynura procumbens (Lour.) Merr. Furthermore, it was proceeded by observing the liver of the experimental animals histopathologically. The acute toxicity test results utilizing the 423 procedures of the OECD did not confirm any death or signs of toxicity in the experimental animals, and histopathological observations did not show any major histopathological damage. Based on these results, according to Globally Harmonized Classification System (GHS), the combination of the ethanol extract of Azadirachta Indica A. Juss. and Gynura procumbens (Lour.) Merr. is included in the unclassified category ( 2,000 mg/kg BW.

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