Sub-chronic oral toxicity study of “Phong Thap Dan” tablets in experimental animal

2021 ◽  
Vol 148 (12) ◽  
pp. 24-31
Author(s):  
Le Thanh Xuan ◽  
Le Thi Nhat Ngoc ◽  
Tran Quang Minh ◽  
Vu Viet Hang ◽  
Pham Thi Van Anh ◽  
...  
Keyword(s):  
Oral Administration ◽  
Experimental Animal ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Toxicity Study ◽  
Biological Parameters ◽  
Oral Toxicity ◽  
Human Dose ◽  
Significant Difference ◽  
Experimental Group

The present study aimed to investigate the sub-chronic toxicity of “Phong thap dan” (PTD) tablets through oral administration in experimental animal. The sub-chronic toxicity was evaluated by the WHO recommendation in Wistar rats at doses of 0.72 g/kg/day (equal to recommended human dose) and 2.16 g/kg/day (3 times as high as recommended human dose). In the sub-chronic experimental group, the PTD was administered orally daily for 8 consecutive weeks. In the evaluation of sub-chronic toxicity, there were no behavioral and physiological change or sign of toxicity. The result of the hematological and biological parameters after administration of PTD tablets showed no change. The histopathology analysis of livers and kidneys indicated that no significant difference was observed between the exposed and unexposed rat groups. In conclusion, “Phong thap dan” tablets did not produce sub-chronic toxicity in Wistar rats.

Sub-chronic oral toxicity study of “Kien Nao Dan” tablets in experimental animal

2021 ◽  
Vol 148 (12) ◽  
pp. 16-23
Author(s):  
Trinh Thi Thuy Hong ◽  
Le Thanh Xuan ◽  
Tran Quang Minh ◽  
Vu Viet Hang ◽  
Pham Thi Van Anh ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Oral Administration ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Physiological Changes ◽  
Biological Parameters ◽  
Oral Toxicity ◽  
Traditional Medicines ◽  
Human Dose ◽  
Histopathologic Analysis

“Kien nao dan” (KND) tablet is composed of 13 traditional medicines that may has preventive and effective treatment of cerebral ischemia. However, there are no scientific reports of its toxicological properties which guarantee of the safety its usage treatment. Therefore, the aim of this study was to investigate the sub-chronic toxicity of KND tablet on rats through oral administration. The sub-chronic toxicity was evaluated by the recommendation of WHO in Wistar rats at doses of 0.72 g/kg/day (equal to recommended human dose) and 2.16 g/kg/day (3 times as high as recommended human dose) for 8 consecutive weeks. In the evaluation of sub-chronic toxicity, there were no behavioral and physiological changes or signs of toxicity. The result of the hematological and biological parameters after administration of KND tablets showed no change. The histopathologic analysis of livers and kidneys indicated that no significant differences were observed between the exposed and unexposed rat groups. In conclusion, “Kien nao dan” tablets did not produce sub-chronic toxicity in Wistar rats.

scholarly journals Evaluation of 90-day subchronic oral toxicity of aqueous extract of Gmelina arborea Roxb (Verbenaceae) leaves in Wistar rats

2020 ◽  
Vol 19 (3) ◽  
pp. 617-622
Author(s):  
Razack Osseni ◽  
Azonbakin Simon ◽  
Diallo Aboudoulatif ◽  
Habib Ganfon ◽  
Adjagba Marius ◽  
...  
Keyword(s):  
Food Intake ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
The Body ◽  
Biological Parameters ◽  
Gmelina Arborea ◽  
Oral Toxicity ◽  
Subchronic Toxicity ◽  
Significant Difference ◽  
Body Weights

Purpose: To evaluate the 90 day sub-chronic toxicity of aqueous extract of Gmelina arborea leaves in Wistar rats. Methods: Rats were submitted to repeated daily oral administration of extract (250, 62.5 and 15.62 mg/kg) of Gmelina arborea leaves. The control groups were given distilled water and the rats were monitored for any toxicity symptoms as well as body and organs weights, water and food intake changes. The biochemical, haematological and histolopathological parameters were analysed. Results: The 90 days administration of the aqueous extract did not produce any toxicity signs or mortality. In addition, no significant alteration in water or food intake by the rats was observed. Although there were no changes in the body weights, significant decrease in the weight of the kidneys of the rats was observed at 250 mg/kg. Biological parameters as well as the histopathology of liver and kidneys were not significantly affected. Significant decreases were noted in glucose level at the three dose levels. In addition, significant difference in the levels of transaminases, glucose and platelets were observed. Conclusion: The 90-days subchronic toxicity test on Gmelina arborea did not produce any toxic effects. This confirms the safety of the plant leaves by traditional medicine practitioners. Keywords: Gmelina arborea, Subchronic toxicity, Wistars rats, Biological parameters

scholarly journals Antihyperglycemic, Endothelial protection and Toxicity study of Basil Leaves Extract on Diabetic Rats

2021 ◽  
Vol 9 (A) ◽  
pp. 589-594
Author(s):  
Sry Suryani Widjaja ◽  
Rusdiana Rusdiana ◽  
Maya Savira ◽  
Rina Amelia
Keyword(s):  
Wistar Rats ◽  
Chronic Toxicity ◽  
Endothelial Activation ◽  
Diabetic Rats ◽  
Toxicity Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Activation Markers ◽  
Toxicity Studies ◽  
Significant Difference

BACKGROUND: Diabetes Mellitus (DM) remains a serious debilitating global health problem in low- and middle-income countries with rising incidence of DM-related complications due to ineffective Diabetic control. Herbs of the Ocimum family, especially Ocimum basilicum or basil leaves, have been investigated for their antihyperglycemic properties. AIM: This study aimed to demonstrate the antihyperglycemic effect, endothelial protection, and toxicity of basil leaves on Diabetes-induced Wistar rats in vivo. METHODS: Streptozosin injections were used to induced diabetes in male Wistar rats. Basil leaves extracts 100, 300, and 1000 mg/kg BW were introduced to diabetic rats. Blood glucose levels (BGL), soluble Advanced Glycation End, tumor necrosis factor-α, interleukin (IL)-6, IL-2 were measured using enzyme-linked immunosorbent assay. Kidney and liver functions together with the histopathology reports were reported for acute, subacute, and chronic toxicity studies. RESULTS: Basil leaves exposure significantly lowers BGL (p < 0.00), but yielded no statistically significant difference between extract doses. Hemostatic parametersshowed significantly reduced endothelial dysfunction markers for all doses compared to control. Toxicity study yielded no differences between control and any doses of basil leaves in all acute, subacute, and chronic toxicity studies. Histopathological findings exhibited no evidence of tissue damage on the liver, kidney, heart, pancreas, lung, and lymph tissues in either control or experiment rats. CONCLUSIONS: Basil leaves exposure were positively associated with lower glucose level, lower endothelial activation markers on Diabetic rats. The toxicity and histopathological results of the extract are on par with control.

scholarly journals Safety Evaluation of Multiple Strains ofLactobacillus plantarumandPediococcus pentosaceusin Wistar Rats Based on the Ames Test and a 28-Day Feeding Study

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Cheng-Chih Tsai ◽  
Sew-Fen Leu ◽  
Quan-Rong Huang ◽  
Lan-Chun Chou ◽  
Chun-Chih Huang
Keyword(s):  
Wistar Rats ◽  
Clinical Chemistry ◽  
Toxicity Study ◽  
Bacterial Strains ◽  
Oral Toxicity ◽  
Short Term ◽  
Mutagenic Potential ◽  
Multiple Strains

Three lactic acid bacterial strains,Lactobacillus plantarum, HK006, and HK109, andPediococcus pentosaceusPP31 exhibit probiotic potential as antiallergy agents, both in vitro and in vivo. However, the safety of these new strains requires evaluation when isolated from infant faeces or pickled cabbage. Multiple strains (HK006, HK109, and PP31) were subject to a bacterial reverse mutation assay and a short-term oral toxicity study. The powder product exhibited mutagenic potential inSalmonellaTyphimurium strains TA98 and TA1535 (with or without metabolic activation). In the short-term oral toxicity study, rats received a normal dosage of 390 mg/kg/d (approximately9×109 CFU/kg/d) or a high dosage of 1950 mg/kg/d (approximately4.5×1010 CFU/kg/d) for 28 d. No adverse effects were observed regarding the general condition, behaviour, growth, feed and water consumption, haematology, clinical chemistry indices, organ weights, or histopathologic analysis of the rats. These studies have demonstrated that the consumption of multiple bacterial strains is not associated with any signs of mutagenicity ofS.Typhimurium or toxicity in Wistar rats, even after consuming large quantities of bacteria.

scholarly journals SUB-CHRONIC TOXICITY STUDY OF THE RAMBUTAN RIND EXTRACT IN MALE WISTAR RATS

2012 ◽  
Vol 10 (2) ◽  
pp. 101-1
Author(s):  
Aree Thinkratok ◽  
Parin Suwannaprapha ◽  
Rungrudee Srisawat
Keyword(s):  
Wistar Rats ◽  
Chronic Toxicity ◽  
Toxicity Study ◽  
Male Wistar Rats

scholarly journals A 90-Day Oral Toxicity of Hydroethanolic Root Extract of Carissa spinarum in Wistar Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Komlan M. Dossou-Yovo ◽  
Aboudoulatif Diallo ◽  
Povi Lawson-Evi ◽  
Yendubé T. Kantati ◽  
Tchin Darré ◽  
...  
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Relative Weight ◽  
Control Group ◽  
Root Extract ◽  
Weight Changes ◽  
Oral Toxicity ◽  
Significant Difference

Background. Herbal medication is a worldwide and ancient practice, mostly in developing countries, where a large part of the population is involved in this practice. Hence, studies must be conducted to evaluate their safety and efficiency to avoid or prevent toxicological risks due to their usage. In Togo, Carissa spinarum is a medicinal plant belonging to Apocynaceae family, used as an aphrodisiac or to heal some ailments including malaria, sickle cell anemia, hypertension, pain, and asthma. Notwithstanding its several ethnomedicinal benefits, just a few toxicological data associated with its chronic use are available. Objective. Therefore, this study aims to assess the toxicity of an ethanolic root extract of Carissa spinarum in Wistar rats. Methods. The 90-day oral toxicity process following OECD TG 408 guidelines is used. Male Wistar rats received Carissa spinarum root hydroethanolic extract at 500 and 1000 mg/kg for 90 days by oral gavage. Body weight changes, hematological and blood biochemical parameters, organ weight changes, malondialdehyde as a lipoperoxidation marker expressed according to tissue proteins, and histopathology of vital organs were assessed. Results. No signs of toxicity or mortality were observed during the 90 days experiment. Hematological parameters have not shown any treatment-related abnormalities. According to biochemical parameters, an increase in the chloride ion level was observed at 1000 mg/kg p < 0.01 . There was no significant difference between the treated groups and the control group concerning the malondialdehyde concentration, body weight, and organ relative weight. No changes in necropsy and histopathology of vital organs associated with extract treatment were observed. Conclusion. The results indicated that an ethanolic root extract of Carissa spinarum does not cause adverse effects, which can lead to Wistar rats’ death after 90-day oral administration at 500 and 1000 mg.

Mesovarial Leiomyomas in Rats in a Chronic Toxicity Study of Soterenol Hydrochloride

1971 ◽  
Vol 8 (5-6) ◽  
pp. 452-457 ◽  
Author(s):  
L. W. Nelson ◽  
W. A. Kelly
Keyword(s):  
Smooth Muscle ◽  
Adrenergic Receptors ◽  
Dose Group ◽  
Chronic Toxicity ◽  
Low Dose ◽  
Toxicity Study ◽  
Ovarian Cysts ◽  
High Dose ◽  
Oral Toxicity ◽  
Β Adrenergic Receptors

In an 18-month oral toxicity study of soterenol hydrochloride, a stimulant of the β-adrenergic receptors, mesovarial leiomyomas were observed in three of 30 low-dose, six of 30 middle-dose and 10 of 30 high-dose rats. There was also an increase in the prevalence of ovarian cysts and of focal hyperplasia of smooth muscle in the mesovaria in the treated rats, especially in the high-dose group.

scholarly journals Repeated dose (90 days) oral toxicity study of ursolic acid in Han-Wistar rats

2020 ◽  
Vol 7 ◽  
pp. 610-623 ◽  
Author(s):  
Lotte Geerlofs ◽  
Zhiyong He ◽  
Sa Xiao ◽  
Zhi-Cheng Xiao
Keyword(s):  
Ursolic Acid ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Repeated Dose ◽  
Oral Toxicity

scholarly journals Acute, Subacute, and Genotoxicity Assessments of a Proprietary Blend of Garcinia mangostana Fruit Rind and Cinnamomum tamala Leaf Extracts (CinDura®)

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Sundararaju Dodda ◽  
Venkata Krishnaraju Alluri ◽  
Trimurtulu Golakoti ◽  
Krishanu Sengupta
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Lethal Dose ◽  
Toxicity Study ◽  
Mouse Bone Marrow ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Garcinia Mangostana ◽  
Cinnamomum Tamala ◽  
Fruit Rind

The present communication describes a battery of toxicity studies that include an acute oral toxicity, a subacute twenty-eight-day repeated oral dose toxicity, and genotoxicity studies on a herbal formulation CinDura® (GMCT). This proprietary herbal composition contains the extracts of the Garcinia mangostana fruit rind (GM) and the Cinnamomum tamala leaf (CT). The toxicological evaluations were performed following the Organization for Economic Cooperation and Development (OECD) guidelines. The acute oral toxicity study in Wistar rats suggests that the median lethal dose of CinDura® is at least 2000 mg/kg body weight. Acute dermal and eye irritation tests in New Zealand white rabbits indicate that the test item is nonirritant to the skin and eyes. A twenty-eight-day repeated dose oral toxicity study was conducted in male and female Wistar rats using daily doses of 250, 500, and 1000 mg/kg body weight, followed by a fourteen-day reversal period for two satellite groups. The CinDura®-supplemented animals did not show any sign of toxicity on their body weights, organ weights, and on the hematobiochemical parameters. The gross pathology and histopathological examinations indicated no treatment-related changes in the experimental animals. Overall, the no-observed-adverse-effect level (NOAEL) of the herbal blend is 1000 mg/kg body weight, the highest tested dose. Also, the results of the bacterial reverse mutation test and the erythrocyte micronucleus assay in mouse bone marrow suggest that CinDura® (GMCT) is neither mutagenic nor clastogenic.

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