scholarly journals Antioxidative, phytochemical and antimicrobial analysis of juices of eight citrus cultivars grown in Pakistan

2022 ◽  
Author(s):  
Sania Naz ◽  
Anila Sajjad ◽  
Joham Ali ◽  
MUHAMMAD ZIA
Keyword(s):  
Free Radical ◽  
Protein Kinase ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Protein Kinase Inhibitors ◽  
Total Flavonoids ◽  
Activity Maximum ◽  
Citrus Juices ◽  
Citrus Varieties

Comparative nutritional analysis of citrus varieties cultivated in Pakistan has not been reported. Citrus is consumed all over the world due to its taste and also has pharmacological components. The present investigation evaluated the antioxidant, reducing power, total flavonoids and phenolics, DPPH free radical scavenging, protein kinase inhibition, and the antimicrobial activities of eight Pakistani citrus varieties. Grapefruit showed maximum total antioxidant potential (77 µg AAE/100 mg), followed by Kinnow and Shakri. Khatai showed maximum reducing power potential (69.6 µg AAE/100 mg) while Shakri and Grapefruit trailed it. All the varieties showed significant DPPH free radical scavenging activity. Maximum total phenolics in citrus juice were found in Shakri and Kinnow; 26.2 and 25.9 µg GAE/100mg, respectively. Variation in total flavonoids content was observed as Kinnow>Grapefruit>Shakri>Khatai. All the citrus juices showed mild to moderate antibacterial activity, while Mosambi and Malta contained potent antifungal components. HPLC analysis of citrus juices revealed that catechin was present in all citrus genotypes except Kinnow. The study concludes that citrus juices contain strong antioxidative potential, bear protein kinase inhibitors and can be used as cancer chemoprevention and supportive nutrition.

scholarly journals Antioxidant Potential, Phenolics Content and Antimicrobial Attributes of Selected Medicinal Plant

2021 ◽  
Vol 22 (2) ◽  
pp. 307-319
Author(s):  
Ali Abbas ◽  
Bushra Sultana ◽  
Anwaar Hussain ◽  
Farooq Anwar ◽  
Naveed Ahmad
Keyword(s):  
Linoleic Acid ◽  
Free Radical ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Flavonoids ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Ethanol Extracts

The bioactive extracts recovered from Curcuma longa (stem), Mentha aquatica (stem and leaves), Emblica officinalis (fruits), Nigella sativa (seeds), and Glycyrrhiza glabra (stem) using methanol and ethanol, were appraised for antioxidant (total flavonoid contents, total phenolic contents, DPPH free radical scavenging ability, inhibition of linoleic acid peroxidation and reducing power) and antimicrobial attributes. Total phenolics (16.89 ± 0.18 - 25.06 ± 0.31g GAE/100g) and total flavonoids (1.96 ± 0.07 - 13.54 ± 0.18 CE g/100g) contents of aqueous methanol extracts of tested plant materials were significantly (p < 0.05) lower than that of aqueous ethanol extracts (13.87 ± 0.12 - 28.63 ± 0.34 g GAE/100g) and (2.20 ± 0.10 - 8.71 ± 0.24 CE /100g), respectively. The percent inhibition of linoleic acid per oxidation by crude ethanol extracts of plants was in the range of 48.72 ± 1.24 - 70.79 ± 1.57 and crude methanol extract 35.90 ± 1.28 - 61.54 ± 2.14, while the range of DPPH free radical scavenging activity of ethanol extracts was (58.36 ± 1.98 - 80.55 ± 3.07) and methanol extracts (39.55 ± 1.52 - 79.64± 2.33). The reducing power of the tested extracts obtained by ethanol (at the concentration of 10 mg/mL) ranged 1.11 ± 0.12 – 1.53 ± 0.18 while for methanol extracts 0.98 ± 0.11 – 1.39 ± 0.12. Among the attributes analyzed, total phenolics, total flavonoids, and DPPH free scavenging activity were found to be varied significantly in all the tested medicinal plants. The extracts from all plants showed good antimicrobial potential against a penal of bacteria, including E. coli, P. multocida and S. aureus, and fungi including A. niger, A. flavus, A. alternate, and G. lucidium. Overall, the tested samples were found to be a good source of natural antioxidant and antimicrobial agents and thus can be explored for potential functional food and nutra-pharmaceutical applications.

scholarly journals Characteristics and upregulation of antioxidant capacity of fermented pueraria starch production wastewater with kombucha consortium

2021 ◽  
Vol 251 ◽  
pp. 02054
Author(s):  
Zhixin Lu ◽  
Hongting Jiang ◽  
Zhongqiang Chen ◽  
Yin Zhai ◽  
Fahu Yuan
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Mass Concentration ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Flavonoids ◽  
Before And After ◽  
Radical Scavenging Ability ◽  
Starch Production

Pueraria lobata is a traditional plant used for both medicine and food. Pueraria starch has a long history of folk consumption in China. The processing of pueraria starch produces a lot of waste water and is often discarded, but it contains rich nutrients and is suitable for the growth of microorganisms. In this study, pueraria starch processing wastewater was used as a new fermentation substrate for kombucha fungus to develop a new functional beverage. After 8 days of static fermentation in 28 °C, the pH of kombucha fermented with pueraria starch processing wastewater as substrate decreased to 2.78, the total acid concentration was 0.158 mol/L, and the mass concentration of reducing sugar decreased to 2.05 mg/mL. The pueraria starch production wastewater before and after fermentation was extracted with 80% methanol and the mass concentration of total flavonoids was determined. The antioxidant activity of pueraria starch processing wastewater before and after fermentation was analyzed by using three antioxidant models: DPPH free radical scavenging ability, ABTS cationic free radical scavenging ability and ferrous ion reducing power. The results showed that after 7 days of fermentation, the mass concentration of total flavonoids in kombucha produced by the pueraria starch processing wastewater was 268.45 mg/L. The antioxidant activity of fermented kombucha beverage was significantly higher than that of unfermented pueraria starch processing wastewater. This study provides a new way for the resource utilization of Pueraria starch processing wastewater.

scholarly journals Antiradical and Cytotoxic Activities of Varying Polarity Extracts of the Aerial Part ofEuphorbia hirtaL.

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Shanmugapriya Perumal ◽  
Roziahanim Mahmud ◽  
Suthagar Pillai Piaru ◽  
Wei Cai Lee ◽  
Surash Ramanathan
Keyword(s):  
Linoleic Acid ◽  
Free Radical ◽  
Aerial Part ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Uv Spectrophotometry ◽  
Etoh Extract

Euphorbia hirtais a well-known ethnomedicinal plant with diverse biological activities. The aim of the present study is to investigate the antiradical activities of various solvent extracts of the aerial part ofE. hirtaas well as to determine the possible cytotoxicity of these extracts. The aerial part ofE. hirtawas extracted with different solvent systems in order to increase polarity. The solvents used were hexane, dichloromethane (DCM), ethyl acetate (EA), ethanol (EtOH), and methanol (MeOH). The contents of total phenols and total flavonoids were analyzed by UV spectrophotometry, whereas the potential free radical-scavenging activities of the extracts were evaluated using the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), theβ-carotene-linoleic acid bleaching system, and reducing power. The EtOH extract exhibited the highest total phenolic content (237.9±2.26 mg GAE/g), and DCM extract scored the highest total flavonoid content (121±0.15 mg CE/g). The MeOH extract showed a potent free radical-scavenging activity as evidenced by low EC50at 42.81 µg/mL. Interestingly, the EtOH extract demonstrated the highest reducing power activity with EC50value of 6.18 µg/mL. Inβ-carotene-linoleic acid assay, oxidation of linoleic acid was effectively inhibited by DCM extract with96.15±0.78%. All the extracts showed no cytotoxic activity against Vero cells.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

scholarly journals Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

2020 ◽  
Vol 8 (1) ◽  
pp. 75-81
Author(s):  
Bui Van Hoai ◽  
Ngo Dai Nghiep ◽  
Dao An Quang ◽  
Nguyen Thi Nam Phuong
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Free Radical ◽  
Trichoderma Viride ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Degree Of Deacetylation ◽  
Radical Scavenging Ability

Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

scholarly journals Aktivitas Antioksidan Minuman Daun Beluntas Teh Hitam (Pluchea indica Less-Camelia sinensis)

2018 ◽  
Vol 38 (2) ◽  
pp. 200
Author(s):  
Paini Sri Widyawati ◽  
Tarsisius Dwi Wibawa Budianta ◽  
Yesiana Dwi Wahyu Werdani ◽  
Maria Olivia Halim
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Black Tea ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Iron Ion

The research was conducted to explore the potency of pluchea leaves-black tea drink as antioxidant at various proportions. The research used a single factor randomized block design of pluchea leaves and black tea proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w). Each of it was repeated five times. The parameters observed in this study were secondary metabolites, total phenolic, total flavonoids, free radical DPPH scavenging activity, and iron reducing power. The data were statistically analyzed using Analysis of Variance (ANOVA) at α = 5%, if the analysis showed a significant effect then it was continued with Duncan's Multiple Range Test (DMRT). Data was stated as mean ± standard deviation. The results showed that the secondary metabolites containing in drink from pluchea leaves and black tea at various proportions were alkaloids, flavonoids, phenolics, saponins, tannins, and cardiac glycosides. The increasing of black tea proportion in samples added the intensity of alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides compounds detected, but the tannins were decreased. These secondary metabolites were correlated with total phenolic content (TPC) and total flavonoid content (TFC). The increasing of black tea proportion in drink significantly decreased DPPH free radical scavenging activity and iron ion reducing power, except for 100% black tea proportion. Tannin compounds seems determining antioxidant activity.  Based on coefficient correlation between TPC or TFC and DPPH free radical scavenging activity or iron ion reducing power, the result showed that DPPH free radical scavenging activity was dominantly contributed by TPC and iron ion reducing power was determined by TPC and TFC. ABSTRAKPenelitian ini dilakukan untuk mengetahui potensi minuman daun beluntas teh hitam sebagai antioksidan pada berbagai proporsi. Penelitian ini menggunakan rancangan acak kelompok satu faktor, yaitu proporsi daun beluntas teh hitam meliputi 100:0; 75:25; 50:50; 25:75; dan 0:100% (b/b). Tiap faktor diulang sebanyak 5 kali. Parameter yang diamati pada penelitian ini meliputi kandungan metabolit sekunder, total fenolik (TPC), total flavonoid (TFC), kemampuan menangkal radikal bebas DPPH, dan kemampuan mereduksi ion besi. Data dianalisis secara statistik dengan Analysis of Variance (ANOVA) pada α = 5%, jika terdapat beda signifikan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Data dinyatakan dengan rata-rata ± standar deviasi.  Hasil menunjukkan bahwa metabolit sekunder yang terkandung dalam minuman daun beluntas teh hitam pada berbagai proporsi adalah alkaloid, flavonoid, fenolik, saponin, tannin, dan kardiak glikosida. Peningkatan proporsi teh hitam menambah intensitas senyawa alkaloid, fenolik, flavonoid, saponin, dan kardiak glikosida yang terdeteksi, tetapi kandungan senyawa tannin berkurang. Kandungan metabolit sekunder ini berkorelasi dengan total fenolik (TPC) dan total flavonoid (TFC). Peningkatan proporsi teh hitam dalam minuman menurunkan kemampuan menangkal radikal bebas DPPH dan kemampuan mereduksi ion besi, kecuali pada proporsi teh hitam 100%. Kandungan senyawa tannin dalam minuman menentukan aktivitas antioksidan. Berdasarkan koefisien korelasi antara TPC atau TFC dan kemampuan menangkal radikal bebas DPPH atau kemampuan mereduksi ion besi, hasil menunjukkan bahwa kemampuan menangkal radikal bebas DPPH dominan dikontribusi oleh TPC dan kemampuan mereduksi ion besi ditentukan oleh TPC dan TFC.

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

Evaluation of antioxidant activity of Melothria maderaspatana in vitro

2010 ◽  
Vol 5 (2) ◽  
pp. 224-230 ◽  
Author(s):  
Boobalan Raja ◽  
Kodukkur Pugalendi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Melothria Maderaspatana

AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant.

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