scholarly journals Nutrients, Fiber Fraction, and In Vitro Fiber Digestibility of Brown-Midrib Sorghum Mutant Lines Affected by The Maturity Stages

2021 ◽  
Vol 44 (3) ◽  
pp. 297-306
Author(s):  
R. Sriagtula ◽  
P. D. M. H. Karti ◽  
L. Abdullah ◽  
Supriyanto Supriyanto ◽  
D. A. Astuti ◽  
...  
Keyword(s):  
Brown Midrib ◽  
Maturity Stages ◽  
Fiber Digestibility ◽  
Fiber Fraction ◽  
Mutant Lines

scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Lactobacillus Brevis ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Aerobic Stability ◽  
Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

scholarly journals Effect of drought stress on in vitro neutral detergent fiber digestibility of corn for silage

2021 ◽  
Vol 273 ◽  
pp. 114803
Author(s):  
G. Ferreira ◽  
L.L. Martin ◽  
C.L. Teets ◽  
B.A. Corl ◽  
S.L. Hines ◽  
...  
Keyword(s):  
Drought Stress ◽  
Neutral Detergent Fiber ◽  
Fiber Digestibility

scholarly journals Dynamic analysis of pulsed cisplatin identifies effectors of resistance in lung adenocarcinoma

2019 ◽  
Author(s):  
Jordan F. Hastings ◽  
Alvaro Gonzalez-Rajal ◽  
Jeremy Z.R. Han ◽  
Rachael A. McCloy ◽  
Yolande E.I. O’Donnell ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Lung Adenocarcinoma ◽  
Patient Specific ◽  
Dna Breaks ◽  
Mutation Status ◽  
Cisplatin Sensitivity ◽  
Mutant Lines ◽  
Platinum Chemotherapy

AbstractIdentification of clinically viable strategies for overcoming resistance to platinum chemotherapy in lung adenocarcinoma has been hampered by inappropriately tailored in vitro assays of drug response. Therefore, using a pulse model that closely recapitulates the in vivo pharmacokinetics of platinum therapy, we profiled cisplatin-induced signalling, DNA damage and apoptotic responses across a panel of lung adenocarcinoma cell lines. By coupling this data with real-time, single cell imaging of cell cycle and apoptosis, we show that TP53 mutation status influenced the mode of cisplatin induced cell cycle arrest, but could not predict cisplatin sensitivity. In contrast, P70S6K-mediated signalling promoted resistance by increasing p53/p63 and p21 expression, reducing double-stranded DNA breaks and apoptosis. Targeting P70S6K sensitised both TP53 wildtype and null lines to cisplatin, but not TP53 mutant lines. In summary, using in vitro assays that mimic in vivo pharmacokinetics identified P70S6K as a robust mediator of cisplatin resistance and highlighted the importance of considering somatic mutation status when designing patient-specific combination therapies.

scholarly journals The Effect of Multistage Ammoniation on Fiber Fraction and Digestibility of Maize Stover in vitro

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
K. Dakaew ◽  
A. Abrar ◽  
A. Cherdthong
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Total Volatile ◽  
Maize Stover ◽  
Dry Matter Digestibility ◽  
Fiber Fraction ◽  
Total Volatile Fatty Acid

The main objective of this experiment was to assess the effect of multistage ammoniation on fiber fraction and digestibility of maize stover in vitro. Maize stover was treated by a multistage technique of different urea connect ration (8%, 4%, and 2%) and incubation for 14 days at room temperature.Digestibility of NDF, ADF, ADL, hemicellulose, and cellulose, in vitro dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N, total volatile fatty acid (VFA) and pH concentration were observed. However treatment maize stover and multistage ammoniation maize stover were composed by using T-test student as the statistic. There were not significantly different on fiber fraction and digestibility of maize stover. However, multistage ammoniation decreased ADF fraction by 56.67% to45.39%. The DM digestibility of multistage ammoniation on fiber fraction and digestibility of maize stover also increased by 35.18 to 45.91. The total VFA and N-ammonia of multistage ammoniation on fiber fraction and digestibility of maize stover also higher than control.Keywords: Ammoniation, Digestibility, in vitro, Maize stover

Detection of P-glycoprotein in ovarian cancer: a molecular marker associated with multidrug resistance.

1985 ◽  
Vol 3 (3) ◽  
pp. 311-315 ◽  
Author(s):  
D R Bell ◽  
J H Gerlach ◽  
N Kartner ◽  
R N Buick ◽  
V Ling
Keyword(s):  
Ovarian Cancer ◽  
Multidrug Resistance ◽  
Resistance Mutation ◽  
Advanced Ovarian Cancer ◽  
Surface Glycoprotein ◽  
Glycoprotein P ◽  
Cell Surface Glycoprotein ◽  
P Glycoprotein ◽  
Mutant Lines

A multidrug resistance phenotype is frequently observed in animal and human cell lines selected for in vitro resistance to a single chemotherapeutic agent. Overexpression of a highly conserved cell-surface glycoprotein (P-glycoprotein) is consistently associated with this phenotype in these mutant lines. A monoclonal antibody against P-glycoprotein was used to examine tumor samples from five patients with advanced ovarian cancer for evidence of P-glycoprotein overexpression. High levels of P-glycoprotein were detected in samples from two patients suggesting that a multidrug resistance mutation may also occur in ovarian cancer. This finding has broad implications for the understanding of nonresponse to chemotherapy in a variety of human neoplasms, and may provide a rational explanation for failure of chemotherapy in treatment of advanced ovarian cancer.

Functional and Hypoglycemic Properties of Nopal Cladodes (O. ficus-indica) at Different Maturity Stages Using in Vitro and in Vivo Tests

2013 ◽  
Vol 61 (46) ◽  
pp. 10981-10986 ◽  
Author(s):  
María A. Nuñez-López ◽  
Octavio Paredes-López ◽  
Rosalía Reynoso-Camacho
Keyword(s):  
Maturity Stages ◽  
In Vivo Tests
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