Purification of the Acetyl CoACarboxylase-1 from Serum of Breast Cancer Women after Mastectomy
2021 ◽
Vol 2
(4)
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pp. 32-42
Keyword(s):
Acetyle CoA Carboxylase-1was purified from serum of premenop- ausal women with breast cancer (after Mastectomy or treatment ) by Gel Filtration using Sephadex G-100 and by Ion Exchange using DEAE-Cellulose A-50, also the molecular weight was estimated by the Electrophoresis on Acrylamide in the absence of denaturing elements . The result showed that a single band was obtained at 210 KD by Gel Filtering while Ion Exchange showed one band at 210 KD. The optimum temperature of purified Acetyle CoA Carboxylase-1 was 40 , optimal pH at 7.5 and the optimum substrate concentration at 1.8mM. Michaelis-Menten constant (km) was 0.3mM and Vmax was 23mM.min-1.
1984 ◽
Vol 62
(6)
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pp. 449-455
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1985 ◽
Vol 63
(11)
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pp. 1160-1166
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1999 ◽
Vol 54
(7-8)
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pp. 496-500
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1984 ◽
Vol 62
(5)
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pp. 276-279
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Keyword(s):