scholarly journals Suitability of tetrazolium test for Tamarindus indica L. seeds

2022 ◽  
Vol 43 (2) ◽  
pp. 509-522
Author(s):  
Luciana Nunes Cordeiro ◽  
◽  
Riselane de Lucena Alcântara Bruno ◽  
Lucas Kennedy Silva Lima ◽  
Albericio Pereira de Andrade ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seed Viability ◽  
Dry Weight ◽  
Tetrazolium Salt ◽  
Tamarindus Indica ◽  
Test Protocol ◽  
Randomized Design ◽  
Class 1 ◽  
Set Up ◽  
Tetrazolium Test

Seed quality is routinely assessed by direct tests, e.g, the germination test, or indirect tests like the tetrazolium test, which has shoown to be promising in the determine viability and vigor, allowing the diagnosis of the main problems that may affect seed quality, such as mechanic damages, field deterioration and storage. In this respect, this study was conducted to develop a tetrazolium test protocol to evaluate the viability and vigor of Tamarindus indica L. seeds. Before exposing the seeds to the tetrazolium solution, seed preconditioning studies were carried out in which seven soaking times were tested. The soaking time that did not cause damage to the seed embryo and allowed the removal of the seed coat to expose the seed structures to the tetrazolium salt was selected. Then, an experiment was set up in a completely randomized design with a 2x3x3 factorial arrangement involving two seed lots, three soaking times in tetrazolium salt (6, 12 and 16 h) and three salt concentrations (0.075, 0.1 and 0.5%), totaling 18 treatments with four replicates of 25 seeds, evaluated at 40 ºC. For each treatment, the seeds were divided into three classes, namely, viable and vigorous embryos (class 1); viable embryos (class 2) and non-viable or dead embryos (class 3). For a comparison with the tetrazolium test results, the two seed lots were analyzed for water content, germination, emergence, first count, germination speed index, emergence speed index, growth and seedling dry weight. The viability and vigor of T. indica seeds can be evaluated after a soaking period of 48 h and immersion for 6h in tetrazolium salt at the concentration of 0.1%, at 40°C, with provides results similar to conventional seed viability tests. The tetrazolium test proved to be adequate to differentiate T. indica seed lots in terms of viability.

scholarly journals Enhancing the Viability of Rosella Seeds (Hibiscus sabdariffa L.) through Tetrazolium and Paper Media Test

2019 ◽  
Vol 4 (1) ◽  
pp. 23-30
Author(s):  
Taufiq Hidayat RS ◽  
Mayasari Yamin
Keyword(s):  
Seed Viability ◽  
Dry Weight ◽  
Tetrazolium Salt ◽  
Hibiscus Sabdariffa ◽  
Germination Capacity ◽  
Randomized Design ◽  
Vigor Index ◽  
Normal Seedling ◽  
Best Parameter ◽  
Viable Seeds

This study was aimed to determine the viability rate of rosella seed, to obtain the best result of seed testing for enhancing rosella seed viability, to find the best seed invigoration method for enhancing rosella seed viability, to obtain staining pattern through tetrazolium test of rosella seed, and to determine viability and vigor of rosella seed to be further used as estimation indicator for rosella plant growth in the field. The study was conducted in the Seed Laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI), Malang, East Java during July - August 2018. The material used included accessions of rosella seed (Hibiscus sabdariffa L.) ACC. 1148 from the year 2015 and collection of ISFCRI, 100 ml of Tetrazolium solution (40 ml KH2PO4, 60 ml Na2HPO4 and 1 gr of Tetrazolium powder). This research applied Completely Randomized Design (CRD) consisted of seed treatments of control (no immersion/0 hour), immersion for 5 hours, immersion for 10 hours, and scarification, each with 4 replications. Result of this study showed that the use of tetrazolium salt was found to be better in enhancing the viability of rosella seeds. Viable seeds was found to have bright red embryonic axis and bright red cotyledon. Testing using paper media on several seed invigoration treatments resulted in significantly different effect on parameters of vigor index, germination capacity, and dry weight of normal seedling. The best parameter of germination capacity and dry weight of normal seedling was obtained by treatment immersed in water at temperature of 27°C for 10 hours

scholarly journals Tetrazolium: an important test for physiological seed quality evaluation

2019 ◽  
Vol 41 (3) ◽  
pp. 359-366 ◽  
Author(s):  
José de Barros França-Neto ◽  
Francisco Carlos Krzyzanowski
Keyword(s):  
Quality Evaluation ◽  
Seed Quality ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Tetrazolium Salt ◽  
High Quality ◽  
Physiological Quality ◽  
The 19Th Century ◽  
Important Test ◽  
Tetrazolium Test

Abstract: The production of high quality seeds is linked to a good quality control system. In this system, the tetrazolium test plays an important role in the evaluation of the seed physiological quality, not only due to its relative quickness, but also because of the amount of information that the test presents, such as the indexes of viability and vigor, in addition to providing the diagnosis of possible seed quality problems, such as mechanical damage, insect damage, pre-harvest weathering and deterioration during storage. The development of the test had its beginnings at the end of the 19th century, with great evolution in its concepts and methods in the 20th century. For that, there was the dedication of dozens of professionals in several countries, such as Germany, Japan, Russia, Serbia (former Yugoslavia), United States, Argentina and Brazil. The test indirectly measures the respiration processes that occur in the mitochondria of the cells that make up the tissues of the seeds. The reducing reaction of the solution of the tetrazolium salt under the action of dehydrogenase enzymes results in triphenylformazan, which presents a red carmin coloration. By the interpretation of the resulting staining patterns, seed viability, vigor and the main problems affecting seed quality are determined.

scholarly journals TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY

2018 ◽  
Vol 31 (4) ◽  
pp. 817-822 ◽  
Author(s):  
Antônia Adailha Torres Souza ◽  
Narjara Walessa Nogueira ◽  
Salvador Barros Torres ◽  
Clarisse Pereira Benedito ◽  
Maria Lilia de Souza Neta ◽  
...  
Keyword(s):  
Test Procedure ◽  
Seed Viability ◽  
Abelmoschus Esculentus ◽  
Tetrazolium Salt ◽  
Distilled Water ◽  
Germination Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tetrazolium Test

ABSTRACT This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an 18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

scholarly journals Adaptation of the tetrazolium test method for estimating the viability of sorghum seeds

2014 ◽  
Vol 36 (2) ◽  
pp. 246-252 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Camila Ribeiro de Souza Grzybowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Quality Control ◽  
Filter Paper ◽  
Seed Quality ◽  
Seed Viability ◽  
Test Method ◽  
Accurate Assessment ◽  
Efficient Procedure ◽  
Paper Towels ◽  
Direct Immersion ◽  
Tetrazolium Test

The tetrazolium test is part of internal programs of seed quality control because it allows a quick, reliable and accurate assessment of seed viability. The purpose of this study was to determine, among the various methodologies suggested in the literature, a practical and efficient procedure for carrying out the tetrazolium test in sorghum seeds, with a view toward a rapid estimate of their viability. Four seed lots of the simple hybrid Buster were used, testing two forms of seed pre-conditioning (direct immersion in water and between moistened paper towels); two types of preparation (longitudinal cut of the seed through the embryo with immersion of one of the halves in the tetrazolium solution or placement of the two halves on filter paper moistened with tetrazolium solution); two forms of staining (on paper and with direct immersion in the solution) and three concentrations of tetrazolium solution (0.1%, 0.5% and 1.0%). The tetrazolium test may be efficiently conducted for sorghum seeds through pre-conditioning between paper towels for 18 hours at 20 ºC, with immersion staining of one half of the seed in tetrazolium solution at 0.1% for three hours at 40 ºC.

scholarly journals Endogenous Hormones in Habanero Pepper Seeds

2021 ◽  
pp. 9-18
Author(s):  
H. Ramírez ◽  
U. A. Macías-Castillo ◽  
A. I. Melendres-Alvarez ◽  
M. C. Castillo-Robles ◽  
A. Zermeño-González ◽  
...  
Keyword(s):  
Seed Viability ◽  
Dry Weight ◽  
Zeatin Riboside ◽  
Human Society ◽  
Endogenous Hormones ◽  
Vegetable Crops ◽  
Randomized Design ◽  
Habanero Pepper ◽  
Freeze Dried ◽  
Statistical Program

Aims: Habanero pepper (Capsicum annuum L.) cultivation is growing worldwide, mainly as a result of its high demand and nutritive contribution to human society. The presence of endogenous gibberellins and cytokinins in seeds of various vegetable crops has been related to a good germination; however, little is known on habanero pepper. The aim of this study was to search for the presence, identification and amount of gibberellins and cytokinins in seeds of habanero pepper cv “Jaguar”. Study design: Laboratory analysis for gibberellins and cytokinins were organized in solvent solutions groups with three technical replicates using a complete randomized design and results when applicable were analyzed using the statistical program 'RStudio' (version 10) and data obtained subjected to a comparison of means with the Tukey (P≤0.05) test. Place and duration of study: The experiment was conducted at the Department of Horticulture in Universidad Autónoma Agraria Antonio Narro, Saltillo, Mexico, during 2020-2021. Methodology: Lots of 50 grams dry weight of “Jaguar” habanero pepper seed samples were freeze dried and prepared with several organic solvents for the extraction, purification and identification of gibberellins and cytokinins using the techniques of GC-MS and GCMS-SIM respectively. Results: Gibberellins A1, A4, A7, A9, A15, A17 and A44 were found in habanero seed tissue. The cytokinins zeatin and zeatin-riboside were also detected in analyzed habanero samples.  Conclusion: The endogenous gibberellins A1, A4, A7, A9, A15, A17, A44, and the cytokinins zeatin and zeatin-riboside are present in habanero pepper cv “Jaguar” contributing to an improve seed viability, ensuring health and overall plant yield.

scholarly journals Short Communication: Tetrazolium test for evaluating viability of Capsicum annum seeds

2018 ◽  
Vol 10 (3) ◽  
pp. 142-145
Author(s):  
ADITYA KUSUMAWARDANA ◽  
BAMBANG PUJIASMANTO ◽  
PARDONO PARDONO
Keyword(s):  
Plant Growth ◽  
Laboratory Test ◽  
Seed Quality ◽  
Seed Viability ◽  
Test Results ◽  
Capsicum Annum ◽  
Laboratory Test Results ◽  
Tetrazolium Staining ◽  
Growth Vigor ◽  
Tetrazolium Test

Kusumawardana A, Pujiasmanto B, Pardono. 2018. Tetrazolium test for evaluating viability of Capsicum annum seeds. Nusantara Bioscience 10: 142-145. Seed quality is important in seeds production. This research was conducted to obtain information of topographical tetrazolium staining pattern of pepper (Capsicum annum) seeds. Tetrazolium test was conducted to determine the seed viability and plant growth vigor. Laboratory test for standard germination and field performances were performed on four lots (A, B, C, D) of pepper seeds. The viability categories pattern were determined by Root Mean Square (RMS), regression, and correlation analyses. Nine topographical patterns were recognized. The laboratory test results and field performances were compared with the topographical pattern. Combination of patterns 1,2 (embryonic axis and cotyledon completely stained) selected as viable category as it gave the least RMS value, the highest determination (R²) and correlation (r) coefficient with standard germination (RMS = 4, 06; R² = 0,761; r = 0,872). Combination of patterns 1,2 also gave the highest determination (R²) and correlation (r) coefficient with field stand (R² = 0,921; r = 0,959). The combination of patterns 1,2 is recommended for estimating plant growth performance in the field.

scholarly journals PENGARUH UMUR PANEN RIMPANG TERHADAP PERUBAHAN FISIOLOGI DAN VIABILITAS BENIH JAHE PUTIH BESAR SELAMA PENYIMPANAN

2016 ◽  
Vol 21 (1) ◽  
pp. 17
Author(s):  
DEVI RUSMIN ◽  
M.R. SUHARTANTO ◽  
SATRIYAS ILYAS ◽  
DYAH MANOHARA ◽  
ENY WIDAJATI
Keyword(s):  
Weight Loss ◽  
Seed Viability ◽  
High Growth ◽  
Dry Weight ◽  
Zingiber Officinale ◽  
Shoot Height ◽  
Harvesting Time ◽  
Seed Technology ◽  
Randomized Design ◽  
Growth Ability

<p>ABSTRAK<br />Salah satu faktor yang menentukan daya simpan benih jahe putih<br />besar (JPB) adalah mutu. Mutu benih sangat ditentukan oleh tingkat<br />kemasakan rimpang. Penelitian bertujuan untuk mengetahui pengaruh<br />umur panen terhadap perubahan fisiologi dan viabilitas benih selama<br />penyimpanan. Penelitian dilaksanakan di Rumah Kaca dan Laboratorium<br />Teknologi Benih, Balai Penelitian Tanaman Rempah dan Obat serta<br />Laboratorium Pascapanen IPB Bogor, mulai bulan Juli 2012 sampai<br />dengan Februari 2013. Rancangan yang digunakan adalah acak lengkap<br />(RAL) dengan lima ulangan. Perlakuan yang diuji adalah tiga tingkat umur<br />panen benih 7, 8, dan 9 bulan setelah tanam (BST). Pengamatan dilakukan<br />terhadap perubahan fisiologis (penyusutan bobot, persentase rimpang<br />bertunas, tunas, kadar air, dan laju respirasi), serta viabilitas rimpang benih<br />(daya tumbuh, tinggi, dan bobot kering bibit). Hasil penelitian<br />menunjukkan rimpang benih umur 7 dan 8 BST mempunyai daya simpan<br />terbaik karena menghasilkan masing-masing total angka penyusutan bobot<br />lebih rendah (24,65 dan 25,25%) dan tunas lebih pendek (0,30 dan 1,08<br />cm) dibandingkan dengan umur panen 9 BST (27,13% dan 1,62 cm),<br />selama 4 bulan disimpan. Masa dormansi rimpang benih JPB mulai pecah<br />setelah mengalami periode simpan 2 bulan. Pertumbuhannya mulai<br />seragam setelah 3 bulan simpan. Umur panen jahe 7 dan 8 BST<br />mempunyai derajat dormansi yang lebih tinggi dibanding 9 BST. Rimpang<br />benih umur panen 7, 8, dan 9 BST mempunyai daya tumbuh tinggi (&gt;95%)<br />dan pertumbuhan bibit seragam setelah 3 bulan disimpan.<br />Kata kunci: Zingiber officinale Rosc., penyimpanan, benih, perubahan<br />fisiologis, viabilitas</p><p>ABSTRACT<br />One of the factors that determine the storability of seed rhizome of<br />white big ginger (WBG) is quality. The quality is determined by the<br />maturity levels of seed rhizome. The aim of the experiment was to observe<br />the effect of harvesting time on physiological changes and seed viability of<br />WBG seed rhizomes during the storage. The experiment was conducted at<br />Green House and Seed Technology Laboratory of Indonesian Spice and<br />Medicinal Crops Research Institute, Bogor and Postharvest Laboratory,<br />IPB, from July 2012 to February 2013. The experiment was arranged in a<br />completely randomized design with five replications. The treatments<br />tested were three levels of WBG seed rhizome harvesting time: 7, 8, and 9<br />month after planting (MAP). Variables observed were physiological<br />changes of seed rhizomes during the storage (weight loss, sprouting<br />percentage, shoot height, respiration rate, and moisture content) and<br />viability (growth ability, height, and dry weight of the seedling). The<br />results showed that seed rhizomes at 7 and 8 had the best storability, since<br />it was produced each low rate of weight loss (24.65 and 25.25%), and<br />shoots shorter (0.3 and 1.08 cm) than 9 MAP (27.13% and 1.62 cm), for 4<br />months in storage. Dormancy of WBG seed rhizomes has been broken<br />after 2 months in storage. Harvesting at 7 and 8 showed a degree of<br />dormancy higher than the harvesting age 9 MAP. Harvesting time at 7, 8,<br />and 9 MAP had high growth ability (&gt; 95%) and uniform seedling growth<br />after 3 months in storage.<br />Keywords: Zingiber officinale Rosc., storage, seed, physiological<br />changes, viability</p>

scholarly journals Growth performance of jute (Corchorus capsularies L.) as influenced by different organic manures

2021 ◽  
Vol 6 (1) ◽  
pp. 17-24
Author(s):  
U Qulsum ◽  
FF Meem ◽  
RS Promi ◽  
JR Zaman ◽  
MF Ara ◽  
...  
Keyword(s):  
Green Manure ◽  
Poultry Manure ◽  
Dry Weight ◽  
Growth And Yield ◽  
Local Market ◽  
Branch Number ◽  
Organic Manures ◽  
Randomized Design ◽  
Different Types ◽  
Set Up

A pot experiment was carried out in 2019 in the net house of the Department of Soil, Water and Environment, University of Dhaka, to evaluate the growth and yield of Jute plants (Corchorus capsularies L.) by applying different types of organic manures. Eight different types of organic manures viz. ACI, BGF-1, Mazim, Vermicompost, Shufola, GTS, Green and Poultry manures were collected from the local market. The experiment was set up following a completely randomized design (CRD) with nine treatments and three replications consisting of twenty-seven pots. Significant variation was observed in different growth and yield contributing characters. The highest plant height (19.83cm) in poultry manure, leaf number (8.33/plant) was observed in Green manure, leaf area (21.93 cm2 / plant) in Poultry manure, fresh weight (3.18g) and dry weight (2.25 g) were found in Poultry manure, stem girth (1.67 cm/plant) in Vermicompost, branch number (8.6/plant) in Green manure. The highest nutrient uptake by plants (kg/ha) occurred in Poultry manure. The overall best growth was observed in Poultry manure. J. Biodivers. Conserv. Bioresour. Manag. 2020, 6(1): 17-24

scholarly journals Effect of botanicals and insecticides seed treatment and containers on seed longevity of black gram under natural ageing conditions

2015 ◽  
Vol 7 (1) ◽  
pp. 328-334 ◽  
Author(s):  
N. Amruta ◽  
G. Sarika ◽  
Umesha Umesha ◽  
J. B. Maruthi ◽  
G. V. Basavaraju
Keyword(s):  
Seed Production ◽  
Seed Treatment ◽  
Seed Quality ◽  
Seed Viability ◽  
Dry Weight ◽  
Management Approach ◽  
Black Gram ◽  
Ambient Conditions ◽  
Emamectin Benzoate ◽  
Production Programme

Good storage is the basic requirement in seed production programme as maintenance of high seed viability and vigour from harvest to planting is of utmost importance in a seed production programme. Therefore, inexpensive, simple and practicable technology to prolong the shelf life of seeds under ambient condition is immensely needed. Hence, an experiment was carried out to study the effect of seed treatment with neem oil, nimbicidin, emamectin benzoate 5 SG, deltamethrin 2.8 EC, novuluron EC on black gram seed quality under ambient conditions. The seeds without any seed treatment were included as control. The treated and untreated seeds were stored in cloth bag and polylined cloth bag. The results revealed that, botanicals and emamectin benzoate seed treatments were significantly superior in controlling the storage insect and maintaining higher seed quality up to 10 months of storage when compared to control. Among the chemicals, the emamectin benzoate 5 SG @ 40 mg kg-1 of seed found better by recording significantly higher germination percentage (80.28), vigour index I (2124) and dry weight of seedlings (43.14 mg) and lower electrical conductivity (0.939 dSm-1) and seed damage (0.00 %) compared to control (78.78%, 2012, 38.90 mg, 0.942 dSm-1, respectively) at the end of 10th month of storage. The emamectin benzoate 5 SG and nimbicidintreated seeds stored in polylined cloth bags were considered as effective seed storage management approach in blackgram.

scholarly journals Criteria for implementation of a tetrazolium test in canola seeds

2015 ◽  
Vol 37 (4) ◽  
pp. 222-227 ◽  
Author(s):  
Mariana Faber Flores ◽  
Camila Ribeiro de Souza Grzybowski ◽  
Kelly Pazolini ◽  
Jean Carlo Possenti ◽  
Maristela Panobianco
Keyword(s):  
Incubation Period ◽  
Crop Rotation ◽  
Vegetable Oil ◽  
Salt Concentration ◽  
Seed Viability ◽  
Human Consumption ◽  
Tetrazolium Salt ◽  
Canola Seed ◽  
Germination Test ◽  
Tetrazolium Test

Canola is an oilseed winter plant, constituting an alternative to crop rotation. It presents socioeconomic value for the production of vegetable oil for human consumption, biodiesel and animal food formulation. The evaluation of canola seed viability is routinely performed by germination test, which can be extended for up to 14 days if it is necessary to overcome dormancy. The objective of this study was to establish criteria for conducting a tetrazolium test in canola seeds, determining viability groups. Four lots of seed were used, placed between soaked paper to hydrate for 16 hours at 20 °C. In the coloration step, after preliminary tests, the following combinations of tetrazolium salt concentration, temperature and incubation period were tested: 0.5 and 1.0% at 30 °C; 0.2, 0.5 and 1.0% at 40 °C for two, four and six hours. It was concluded that the evaluation of viability of canola seeds by the tetrazolium test is effective upon hydration of the seeds between papers for 16 hours at 20 °C, coloration by immersion in 0.5% tetrazolium solution for six hours, at 40 °C and two viability groups were established and illustrated.

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