Quantification of Andrographolide in Andrographis paniculata (Burm.f.) Nees, Myricetin in Syzygium cumini (L.) Skeels, and Brazilin in Caesalpinia sappan L. by HPLC Method

Introduction. Researchers usually use herbal combinations to explore and develop traditional medicine to obtain additional benefits in the treatment of diseases, including diabetes. This study aims to evaluate the hypoglycemic effect of the combination of Andrographis paniculata (Burm. f.) Wall ex Nees and Caesalpinia sappan Linn extract (APCSE) on diabetes-induced rats. There has not been sufficient research on this combination; however, single extract studies of these plants have been widely conducted. Materials and Methods. Male Sprague Dawley rats (160–200 g) were induced by injecting a low dose of streptozotocin (35 mg/kg BW) twice and fed with a high-fat diet containing 25% fat, whereas control animals received only standard feed. Rats were treated with APCSE at doses of 100 mg and 200 mg/kg BW for seven days and compared to the APE and CSE groups treated with the extract at 100 mg, respectively. For the control group, rats were treated with metformin with a dose of 250 mg/kg. The antihyperglycemic and antihyperlipidemic effects were determined by measuring blood glucose levels and lipid profiles (cholesterol, triglycerides, HDL, and LDL). To assess the impact of the extract on pancreatic and adipose tissue, the number of pancreatic beta cells and adipocytes was evaluated through histopathological and immunohistochemical study. Results and Discussion. In a nonfasting state, the blood glucose change in APCSE 200 mg was 18.65% and was significantly lower from the DM group. However, a single extract of APE and CSE showed lower fasting blood glucose levels compared to the combined extract. Lipid profiles show no significant differences in cholesterol levels between groups; however, all treatment groups, including metformin, showed higher triglyceride levels. The APE-treated group showed significantly lower HDL and LDL, whereas CSE only showed lower LDL. The β-cell number was significantly higher after treatment with single extract CSE. The CSE and the combined extract groups showed hyperplasia adipocytes. Conclusion. The combined extract of APCSE has a moderate antihyperglycemic effect; however, a single extract may have better potential than the combined extract.

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Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v14i1.23738 ◽

2015 ◽

Vol 14 (1) ◽

pp. 71-78 ◽

Cited By ~ 4

Author(s):

BK Sajeeb ◽

Uttom Kumar ◽

Shimul Halder ◽

Sitesh C Bachar

Keyword(s):

Quantitative Estimation ◽

Reversed Phase ◽

Hplc Method ◽

Andrographis Paniculata ◽

Plant Sample ◽

Rp Hplc ◽

Ft Ir ◽

Therapeutic Activities ◽

Identification And Quantification

Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 ?g/mL in the solution of amorphous residue (50 ?g/mL). After quantitative determination of andrographolide, standardization of its market preparations was accomplished using the same RP-HPLC method. The estimated % potencies of andrographolide compared to reference standard in six market preparations were found to be 97.56, 98.37, 98.21, 95.60, 98.91 and 96.40.Dhaka Univ. J. Pharm. Sci. 14(1): 71-78, 2015 (June)

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The Pharmacokinetic Drug-Drug Interactions of Andrographis paniculata and Ibuprofen in the Plasma of Healthy Oryctolagus cuniculus Rabbits

Pharmacology and Clinical Pharmacy Research ◽

10.15416/pcpr.v5i2.27508 ◽

2020 ◽

Vol 5 (2) ◽

pp. 40

Author(s):

Mutakin Mutakin ◽

Sandra Megantara ◽

Batari A. Larasati ◽

Yogiyanto Yogiyanto ◽

Jutti Levita ◽

...

Keyword(s):

Oral Administration ◽

Oryctolagus Cuniculus ◽

Hplc Method ◽

Andrographis Paniculata ◽

Mobile Phase Flow Rate ◽

Single Oral Administration ◽

Group 3 ◽

Group 2 ◽

Pharmacokinetic Drug ◽

Group 1

An HPLC method was developed and validated for the pharmacokinetic drug-drug interaction between Andrographis paniculata and ibuprofen in the plasma of Oryctolagus cuniculus rabbits after a single oral administration of the mixture. Nine healthy rabbits (6 males and 3 females, weight 1.68-2.42 kg) were acclimatized for 7 days and were randomly divided into 3 groups. At day-8th the rabbits were group (1) treated with a single oral administration of ibuprofen (dose of 28 mg/kg BW); group (2) treated with a single oral administration of Andrographis paniculata infusion (7.04 mL/kg BW); group (3) treated with a single oral administration of a mixture of Andrographis paniculata (7.04 mL/kg BW) infusion and ibuprofen (dose of 28 mg/kg BW). Plasma samples were prepared by collecting the blood from the marginal ear vein at 0, 30, 60, 90, and 120 minutes after the mixture administration, followed by centrifuging it for 30 minutes 3000 rpm. Chromatographic separation was performed on a LiChrosorb RP-18 with methanol and double-distilled water (70:30) as the mobile phase, flow rate 1 mL/minute. UV detection was set at 227 nm. The absorption and distribution of ibuprofen were fast (Tmax = 30 min; Cmax = 4.02962 mcg/mL), however, interestingly this drug could improve the absorption and distribution of andrographolide in Oryctolagus cuniculus rabbits

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PENGUJIAN VALIDASI ANALISIS KADAR ANDROGRAFOLID SECARA KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) DENGAN ELUASI GRADIEN TERHADAP EKSTRAK HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NESS)

Journal of Biological Researches ◽

10.23869/bphjbr.11.1.200512 ◽

2005 ◽

Vol 11 (1) ◽

pp. 73-76

Author(s):

Toetik Aryani

Keyword(s):

Hplc Analysis ◽

Hplc Method ◽

Andrographis Paniculata ◽

Percent Accuracy

Andrographolide of Andrographis paniculata Nees have been isolated and determined Extraction was carried out by maceration with ethanol as solvent. The concentration of Andrographolide was determined by HPLC method. The eluation was carried by out gradiently using methanol: water as an eluent and UV spectrophotometer at maks 228 as detector. The result of HPLC analysis are selectivity more than 1.2–1.5, r was 0.9937, precision was KV less than 10 percent; accuracy more than 90 percent, DL was 0.075, QL was 0.50 and PW less than F table. Andrographolide content of Andrographis paniculata Nees from Banyuwangi was 6.25 percent, Kediri was 14.69 percent and Surabaya was 6.83 percent.

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