scholarly journals The Determination of Total Alkaloid, Polyphenol, Flavonoid and Saponin Contents of Pogang gan (Curcuma sp.)

2018 ◽  
Vol 10 (4) ◽  
pp. 42
Author(s):  
Phan Van Tan
Keyword(s):  
Total Alkaloid ◽  
Total Content ◽  
Growth Time ◽  
Curcuma Zedoaria ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Total Saponin ◽  
Saponin Content ◽  
Total Alkaloid Content

Pogang gan (Curcuma sp., Zingiberaceae family) is medicinal herb and threatened and it is usually used as a tonic. We successfully micropropagated and cultivated this species. This study determined the total alkaloid, polyphenol, flavonoid and saponin contents of its growth time in order to determine harvest time. The highest level of total alkaloid content is roots after 11 months cultivation (0.272 mg/g), total polyphenol content is rhizomes after 11 months (1.71 mg GAE/g), total flavonoid content is rhizomes after 11 months (13.89 mg QE/g) and total saponin content is tubers and roots after 11 months (5.26% and 4.78%). The total content of alkaloid and flavonoid of pogang gan is higher than Curcuma zedoaria. The total saponin content of natural pogang gan is equal to 39.5% of 6-year old Vietnamese ginseng and the saponin content in rhizomes and tubers of 11-month-planted Pogang gan is from 55.5% to 67.8%, compared with natural Pogang gan.

Validation of Spectrophotometric Methods for the Determination of Total Polyphenol and Total Flavonoid Content

2017 ◽  
Vol 100 (6) ◽  
pp. 1795-1803 ◽  
Author(s):  
Petra Matić ◽  
Marija Sabljić ◽  
Lidija Jakobek
Keyword(s):  
Total Flavonoid ◽  
Chlorogenic Acids ◽  
Flavonoid Content ◽  
Polyphenol Content ◽  
Validation Process ◽  
Total Polyphenol ◽  
Spectrophotometric Methods ◽  
Calibration Curves ◽  
Validation Parameters

Abstract The aim of this study was to validate spectrophotometric methods for the measurement of total polyphenol (TP; via the Folin–Ciocalteu method) and total flavonoid (TF) content [via the aluminum chloride (AlCl3) method]. Validation parameters of these methods were determined, including linearity, sensitivity, precision (intra-assay and intermediate), accuracy, LOD, and LOQ. For the validation process, groups of polyphenol standards were used, including phenolic acids (gallic, p-coumaric, caffeic, and chlorogenic acids), flavan-3-ols [(+)-catechin and procyanidins B1 and B2], flavonols (quercetin and quercetin-3-rutinoside), and dihydrochalcones (phloretin and phloretin-2-glucoside). Obtained validation parameters were within acceptable ranges with high determination coefficients, reasonably low LODs and LOQs, and high slopes in the calibration curves for both methods, except for phloretin and phloretin-2-glucoside, for which there were low slopes in the calibration curves for the AlCl3 method. To evaluate differences in polyphenol content, the validated spectrophotometric methods were used to determine TP and TF content in wines (Plavac, Graševina, and Vranac) and juices (blueberry, strawberry, and blackcurrant juice) according to the polyphenol calibration curves. Polyphenol contents were different for both methods in all wines and juices.

Determination of Antioxidant Components and Activity of Tamarix ramosissima Comparative with Vaccinium myrtillus on Streptozotocin-diabetic Mice

2018 ◽  
Vol 69 (7) ◽  
pp. 1860-1865
Author(s):  
Luciana Teodora Rotaru ◽  
Renata Maria Varut ◽  
Mihai Banicioiu Covei ◽  
Irina Iuliana Costache ◽  
Marius Novac ◽  
...  
Keyword(s):  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Vaccinium Myrtillus ◽  
Tamarix Ramosissima ◽  
Diabetic Mice ◽  
Flavonoid Content ◽  
Thiobarbituric Acid Reactive Substances ◽  
Total Polyphenol

Tamarix ramosissima (Tamaricaceae) is a small tree that grows spontaneously in Europe and Asia, being considered an invasive species in geographical areas with warm climates. The chemical composition is partially elucidated, being empirically used for antiinflammatory, analgesic, antibacterial and antioxidant effect. Our study aimed to evaluate the total polyphenol and flavonoid content of vegetal extracts and to test in vivo antioxidant therapeutic effect of it, comparative with Vaccinium myrtillus, using streptozotocin-induced diabetic mice. After five weeks the animals were sacrificed and we determined erythrocyte activities of superoxide dismutase, glutathione peroxidase, glutathione reductase and level of lipid peroxides as thiobarbituric acid reactive substances. Antioxidant enzymes had highest activities in mice treated with T. ramosissima extract and the level of lipid peroxides was the lowest. The tested extract had higher content of polyphenols comparative with V. myrtillus. Our results sustain the efficiency of T. ramosissima extracts on normalizing the effects of oxidative stress in diabetes.

scholarly journals Determination of flavonoids and total polyphenol contents in commercial apple juices

2018 ◽  
Vol 36 (No. 3) ◽  
pp. 233-238 ◽  
Author(s):  
Leposava Pavun ◽  
Predrag Đurđević ◽  
Milena Jelikić-Stankov ◽  
Daniela Đikanović ◽  
Snežana Uskoković-Marković
Keyword(s):  
Fruit Juice ◽  
Accurate Determination ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Total Polyphenol ◽  
Strong Emission ◽  
Spectrofluorimetric Method ◽  
Good Agreement ◽  
Antioxidative Ability

We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in ‘quercetin equivalent’ in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers.

scholarly journals Assessment of the Total Flavonoid, Phenol, Alkaloid Content and Sun Protection Factor in Grewia abutilifolia Leaf Extract

2021 ◽  
pp. 42-51
Author(s):  
Dolly Rani ◽  
Harsha Kharkwal ◽  
Megha Jha ◽  
Nitin Rai
Keyword(s):  
Total Phenolic Content ◽  
Total Alkaloid ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Alkaloid Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Alkaloid Content

A variety of secondary metabolites provide a valuable source of treatment for various pharmacological ailments. Grewia abutilifolia is a shrub found in the family Tiliaceae that is also investigated for its nutritional and medicinal benefits. Grewia is reported for its good antioxidant potential, which is applied to protect skin from UV rays. The main goal of this study is to determine the in-vitro sunscreen effect of Grewia abutilifolia based on phytochemical evidence of UV protection. Chemical treatments used to protect skin from the sun have several drawbacks, including numerous hazardous side effects on humans and the environment. The hydroalcoholic, ethyl acetate, and methanolic extracts were studied for their phytochemical, physicochemical, and sun protection activities. Total phenolic content, total alkaloid content, total flavonoid content, and SPF value were all highest in the methanolic leaf extract, with values of 99.20 0.12, 47.661.527, 96.02, and 14.50.01 for total phenolic content, total alkaloid content, total flavonoid content, and SPF value, respectively.

scholarly journals Phytochemical Contents and Antioxidant Capacity of the Leaves of Aloe greatheadii var. davyana from Four South African Provinces

2019 ◽  
Vol 31 (8) ◽  
pp. 1830-1834
Author(s):  
Sechene S. Gololo ◽  
Denga Nthai ◽  
Motetelo A. Mogale
Keyword(s):  
South Africa ◽  
Antioxidant Capacity ◽  
Limpopo Province ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Total Saponin ◽  
Saponin Content ◽  
Phytochemical Contents

The current study was aimed at the determination of the phytochemical contents and antioxidant capacity of the leaves of A. greatheadii from four provinces of South Africa. The leaves samples of A. greatheadii were collected at four different locations in South Africa; namely, Limpopo province (Polokwane), Gauteng province (Ga-Rankuwa), Mpumalanga province (Siyabuswa) and North-West province (Brits) during the summer season of the year. The ground samples were then extracted in a serial sequential procedure with different organic solvents using cold maceration extraction method. The extracts were then subjected to quantitative phytochemical analysis for the determination of total phenolic, total tannin, total flavonoid and total saponin content. In addition, the antioxidant capacity of the methanol extracts were evaluated through the DPPH assay. Quantitative phytochemical analysis showed variation in the total phenolic, total tannin, total flavonoid and total saponin contents of the samples from different locations, with specific phytochemicals found in higher amounts at different locations. However, upon calculation of the total phytochemical content the high amount was recorded with the Limpopo province sample. Variation in the antioxidant capacity (EC50 based) of the samples from different locations was demonstrated, with the Limpopo and Mpumalanga provinces’ samples showing high capacity. The results demonstrated that the variation in geographical locations has an effect on the phytochemical contents and antioxidant capacity of the leaves of A. greatheadii. Furthermore, the Limpopo province area with a high altitude appeared to be providing suitable growing conditions for high accumulation of phytochemicals and higher antioxidant capacity.

scholarly journals Effect of Storage Temperature and Preservatives on the Stability and Quality of Polyscias fruticosa (L.) Harms Herbal Health Drinks

2019 ◽  
pp. 1-7
Author(s):  
Nguyen MinhThu ◽  
Hoang Le Son
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Storage Temperature ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Saponin ◽  
Saponin Content ◽  
And Storage

Aims: Polyscias fruticosa has been well-known as a traditional medicinal herb which shares the same function as ginseng, favorable for their antioxidant capacity. In this study, the herbal health drink had been developed based on the Polyscias fruticosa extract. The effect of preservatives and storage temperature on the total phenolic content, total flavonoid content, and total saponin content were investigated over the period of 16 weeks. Methodology: Polyscias fruticosa extract based herbal drinks were formulated. Potassium sorbate and sodium benzoate were used as preservatives while storage temperature was set at 4 and 250C. Determination of total phenolic content was performed by Folin-Ciocalteu method. Meanwhile, analysis of total flavonoid and saponin content was conducted by colorimetric methods. Results: In general, the effect of preservatives and storage temperature on the concentration of total phenolic content and total flavonoid content can clearly be seen after 6 weeks, while significant difference in concentration of total saponin content had been evidenced from week 11. Typically, The concentration of total phenolic content, total flavonoid content and total saponin content in formulas added preservatives and kept at 4°C were measured at 2.80±0.26 mg GAE/g, 8.24±0.44 mg CE/g and 20.29±0.27 mg OAE/g after 16 weeks, respectively; however, without adding preservatives and stored at 25°C, these components were found at a value of 1.77±0.1 mg GAE/g, 0.0±0.28 mg CE/g, 14.63±0.59 mg OAE/g, respectively. Conclusion: Overall, the presence of preservatives and fridge temperature (4°C) has been the optimal condition to maintain the quantity of biological phytocomponents in herbal health drink; however, the addition of preservatives and storage temperature should be taken into consideration depending on the storage time of herbal drink.

scholarly journals Phytochemical Screening and Determination of Polyphenols in the Hydro-Ethanolic Extract of Trunk Bark and Its Fractions of Stereospermum kunthianium Cham (Bignoniaceae)

2021 ◽  
pp. 1-9
Author(s):  
Abdou Sarr ◽  
Serigne Ibra Mbacké Dieng ◽  
Kady Diatta-Badji ◽  
Amadou Ibrahima Mbaye ◽  
William Diatta ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Aluminium Chloride ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Healing Agent

Background : Stereospermum kunthianum Cham., is a medicinal plant from the Bignoniaceae family, used in Ferlo (Senegal) against stomach aches, as a healing agent and aphrodisiac.Aims/Objective : The aim of this study was to contribute to the valorisation of the plant by carrying out a phytochemical screening followed by an assay of the polyphenols of the hydro-ethanolic extract of the bark and its fractions.Methods : Phytochemical screening was carried out by coloring and/or precipitation reactions. The total polyphenol and tannin contents were evaluated by the Folin-Denis method and the flavonoid content by a method using aluminium chloride (AlCl 3 ) and sodium nitrite (NaNO 2 ). Results: The search for chemical compounds revealed the presence of various secondary metabolites such as gallic and catechic tannins, flavonoids, saponosides, alkaloids, sterols and polyterpenes. The total polyphenol contents of the samples ranged from 0.81 ± 0.02 to 13.52 ± 0.06 mg EAT / g. Those of tannins and flavonoids were between 0.03 ± 0.01 and 4.56 ± 0.02 mg EAT / g and between 1.13 ± 0.04 to 31.88 ± 0.19 mg ER / g respectively.Conclusion : These different metabolites would be responsible for the activities noticed for this plant.

Determination of arbutin, rutin, total content of phenols and antioxidant capacity in fruits and leaves of lingonberry, Vaccinium vitis-idaea L. (Ericaceae)

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
J Krvavac ◽  
E Kovac-Besovic ◽  
J Toromanovic ◽  
M Salihovic ◽  
I Tahirovic ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Content
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