scholarly journals The effect of storage temperature of cucumber fruit on chlorophyll fluorescence

2013 ◽  
Vol 53 (1) ◽  
pp. 57-64
Author(s):  
Ryszard Kosson
Keyword(s):  
Chlorophyll Fluorescence ◽  
Storage Temperature ◽  
Optimum Level ◽  
Variable Fluorescence ◽  
Fluorescence Measurements ◽  
Cucumber Fruit ◽  
Variable Chlorophyll Fluorescence ◽  
Relative Variable ◽  
Temperature Levels ◽  
Relative Variable Fluorescence

The effect of three storage temperature levels: 12,5°C, 20°C, and 1,5°C on basic indexes of chlorophyll fluorescence of cucumber fruits was studied. The greenhouse grown cucumber fruits cv. Wiktor F1 were stored in perforated polyethylene bags or without packages. The minimum chlorophyll fluorescence (Fo), maximum chlorophyll fluorescence (Fm), variable chlorophyll fluorescence (Fv) and relative variable fluorescence (Fv/Fm) of the cucumber peel were measured. Relative variable fluorescence was decTeasing when cucumbers were stored at temperature lower or higher than optimum level. The chlorophyll fluorescence measurements can be helpful for determination of appropriate temperature parameters of cucumber storage.

scholarly journals Ascorbic Acid-Induced Photosynthetic Adaptability of Processing Tomatoes to Salt Stress Probed by Fast OJIP Fluorescence Rise

2021 ◽  
Vol 12 ◽  
Author(s):  
Xianjun Chen ◽  
Yan Zhou ◽  
Yundan Cong ◽  
Pusheng Zhu ◽  
Jiayi Xing ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Salt Stress ◽  
Excitation Energy ◽  
Fluorescence Intensity ◽  
Heat Dissipation ◽  
Variable Fluorescence ◽  
Fluorescence Rise ◽  
Relative Variable ◽  
Relative Variable Fluorescence ◽  
Processing Tomatoes

In this study, the protective role of exogenous ascorbic acid (AsA) on salt-induced inhibition of photosynthesis in the seedlings of processing tomatoes under salt stress has been investigated. Plants under salt stress (NaCl, 100 mmol/L) were foliar-sprayed with AsA (0.5 mmol/L), lycorine (LYC, 0.25 mmol/L, an inhibitor of key AsA synthesis enzyme l-galactono-γ-lactone dehydrogenase activity), or AsA plus LYC. The effects of AsA on fast OJIP fluorescence rise curve and JIP parameters were then examined. Our results demonstrated that applying exogenous AsA significantly changed the composition of O-J-I-P fluorescence transients in plants subjected to salt stress both with and without LYC. An increase in basal fluorescence (Fo) and a decrease in maximum fluorescence (Fm) were observed. Lower K- and L-bands and higher I-band were detected on the OJIP transient curves compared, respectively, with salt-stressed plants with and without LYC. AsA application also significantly increased the values of normalized total complementary area (Sm), relative variable fluorescence intensity at the I-step (VI), absorbed light energy (ABS/CSm), excitation energy (TRo/CSm), and reduction energy entering the electron transfer chain beyond QA (ETo/CSm) per reaction centre (RC) and electron transport flux per active RC (ETo/RC), while decreasing some others like the approximated initial slope of the fluorescence transient (Mo), relative variable fluorescence intensity at the K-step (VK), average absorption (ABS/RC), trapping (TRo/RC), heat dissipation (DIo/RC) per active RC, and heat dissipation per active RC (DIo/CSm) in the presence or absence of LYC. These results suggested that exogenous AsA counteracted salt-induced photoinhibition mainly by modulating the endogenous AsA level and redox state in the chloroplast to promote chlorophyll synthesis and alleviate the damage of oxidative stress to photosynthetic apparatus. AsA can also raise the efficiency of light utilization as well as excitation energy dissipation within the photosystem II (PSII) antennae, thus increasing the stability of PSII and promoting the movement of electrons among PS1 and PSII in tomato seedling leaves subjected to salt stress.

scholarly journals Optical Properties of Corals Distort Variable Chlorophyll Fluorescence Measurements

2019 ◽  
Vol 179 (4) ◽  
pp. 1608-1619 ◽  
Author(s):  
Daniel Wangpraseurt ◽  
Mads Lichtenberg ◽  
Steven L. Jacques ◽  
Anthony W. D. Larkum ◽  
Michael Kühl
Keyword(s):  
Optical Properties ◽  
Chlorophyll Fluorescence ◽  
Fluorescence Measurements ◽  
Variable Chlorophyll Fluorescence

Structural and functional disorder in the photosynthetic apparatus of radish plants under magnesium deficiency

2018 ◽  
Vol 45 (6) ◽  
pp. 668 ◽  
Author(s):  
Izabela A. Samborska ◽  
Hazem M. Kalaji ◽  
Leszek Sieczko ◽  
Vasilij Goltsev ◽  
Wojciech Borucki ◽  
...  
Keyword(s):  
Magnesium Deficiency ◽  
Photosynthetic Apparatus ◽  
Dissipated Energy ◽  
Reaction Centre ◽  
Variable Fluorescence ◽  
Mg Deficiency ◽  
Chl A ◽  
Chl A Fluorescence ◽  
Relative Variable ◽  
Relative Variable Fluorescence

Magnesium (Mg) is one of the significant macronutrients which is involved in the structural stabilisation of plant tissues and many enzymes such as PSII. The latter efficiency and performance were analysed, using chlorophyll (Chl) a fluorescence induction kinetics and microscopic images, to detect the changes in structure and function of photosynthetic apparatus of radish plants grown under Mg deficiency (Mgdef). Plants grown under Mgdef showed less PSII connectivity and fewer active primary electron acceptors (QA) oxidizing reaction centres than control plants. Confocal and electron microscopy analyses showed an increased amount of starch in chloroplasts, and 3,3ʹ-diaminobenzidine (DAB)-uptake method revealed higher H2O2 accumulation under Mgdef. Prominent changes in the Chl a fluorescence parameters such as dissipated energy flux per reaction centre (DIo/RC), relative variable fluorescence at 150 μs (Vl), and the sum of the partial driving forces for the events involved in OJIP fluorescence rise (DFabs) were observed under Mg deficiency. The latter also significantly affected some other parameters such as dissipated energy fluxes per cross-section (DIo/CSo), performance index for energy conservation from photons absorbed by PSII antenna until the reduction of PSI acceptors (PItotal), and relative variable fluorescence at 300 μs (Vk). This work emphasises the use of chlorophyll fluorescence in combination with microscopic and statistical analyses to diagnose the effects of nutrients deficiency stress on plants at an early stage of its development as demonstrated for the example of Mgdef. Due to the short growth period and simple cultivation conditions of radish plant we recommend it as a new standard (model) plant to study nutrients deficiency and changes in plant photosynthetic efficiency under stress conditions.

scholarly journals COMPARATIVE HEAT TOLERANCE AMONG FIVE SPECIES OF BIRCH

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 627d-627
Author(s):  
Thomas G. Ranney
Keyword(s):  
Chlorophyll Fluorescence ◽  
Stomatal Conductance ◽  
Heat Tolerance ◽  
Dark Respiration ◽  
Leaf Gas Exchange ◽  
Betula Papyrifera ◽  
Respiration Rates ◽  
Fluorescence Measurements ◽  
Variable Chlorophyll Fluorescence ◽  
Paper Birch

Leaf gas exchange and chlorophyll fluorescence measurements were used as indices for evaluating heat tolerance among five species of birch: paper (Betula papyrifera), European (B. pendula), Japanese (B. platyphylla var. japonica `Whitespire'), Himalayan (B. jacquemontii), and river (B. nigra). Measurements were conducted on individual leaves at temperatures ranging from 25C to 40C. Carbon exchange rates (CER) were depressed for all species at 40C. However, there was considerable variation in both absolute and relative (percent of maximum) CER among species at 40C; river birch maintained the highest absolute and relative CER while CER of paper birch was reduced the most. Although stomatal conductance of paper birch decreased at higher temperatures, internal leaf CO2 increased indicating that reduced stomatal conductance was not responsible for decreased CER. Stomatal conductance of river birch increased at higher temperatures which provided for enhanced uptake of CO2 and greater evaporative cooling. Variable chlorophyll fluorescence decreased similarly for both species with increasing temperatures. Measurements of dark respiration rates over the range of 25C to 40C suggested that the primary factor influencing variation in CER at higher temperatures was due to variation in respiration rates at higher temperatures.

scholarly journals Foliar Heat Tolerance of Two Hybrid Hollies

HortScience ◽  
1993 ◽  
Vol 28 (6) ◽  
pp. 650-652 ◽  
Author(s):  
John M. Ruter
Keyword(s):  
Chlorophyll Fluorescence ◽  
Plant Growth ◽  
Heat Tolerance ◽  
Growth Temperature ◽  
Electrolyte Leakage ◽  
Variable Fluorescence ◽  
Fluorescence Techniques ◽  
Fluorescence Measurements ◽  
Two Hybrid ◽  
Physiologic Data

Temperatures producing heat damage in leaves of Ilex ×meserveae S.Y. Hu `Blue Prince' and Ilex rugosa × cornuta Lindl. & Paxt. `Mesdob' (China Boy) were evaluated using electrolyte leakage and chlorophyll fluorescence techniques. Whole leaves were exposed to temperatures from 30 to 65C for 30 minutes to determine critical midpoint heat-killing temperatures (TJ using electrolyte leakage techniques. The Tm for `Blue Prince' and `Mesdob' was 52.4 ± 0.lC and 53.8 ± 0.lC, respectively. Dark-adapted leaves were heated for 30 minutes in darkness at temperatures between 30 and 57C before chlorophyll fluorescence was measured. Initial (F0) and peak fluorescence measurements were higher at 54 and 55C for `Mesdob' than for `Blue Prince'. Cultivar had no effect on variable fluorescence (F,). Based on the Fv: Fo ratio, `Mesdob' was estimated to have a higher optimal plant growth temperature than `Blue Prince'. The physiologic data support the hypothesis that I. cornuta as a parent conferred heat tolerance to the interspecific hybrid in this study.

scholarly journals 007 Evaluation of the Spring Frost Susceptibility of Strawberry Genotypes Using Chlorophyll Fluorescence Measurements

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 389A-389
Author(s):  
S. Khanizadeh ◽  
J.R. DeEll ◽  
N. Hakam
Keyword(s):  
Chlorophyll Fluorescence ◽  
Environmental Changes ◽  
Strong Relationship ◽  
Frost Tolerance ◽  
Variable Fluorescence ◽  
Flower Buds ◽  
Dramatic Decrease ◽  
Spring Frost ◽  
Fluorescence Measurements ◽  
Frost Injury

Frost tolerance of flower buds is one of the most important characteristics of strawberry cultivars that produce fruit early or very early in the season. The objective of this study was to evaluate chlorophyll fluorescence (CF) as a suitable rapid method to assess spring frost injury of strawberry flowers. More specifically, to determine if there was a relationship between a decrease in CF and the appearance of visual symptoms (visual expression of necrosis (VEN) based on the amount of dark, damaged, and/or water soaked tissue of the pistil) due to frost. Sixty-six strawberry genotypes with varying levels of chilling susceptibility were used. The plants were grown in a greenhouse under a 16-h light period at 20-22 °C during the daytime and 16-18 °C at night. For the CF and VEN measurements, the plants were stored at -3 °C for 24 h followed by 24 h in the greenhouse. The CF measurements were made on dark-adapted tissue, using the Fv/Fm test of an OS-500 modulated fluorometer. For the VEN method, the flowers which had dark, damaged, and/or water soaked pistils were counted. The results showed that variable fluorescence (Fv) decreased as the temperature was lowered. The spring frost resistant cultivars maintained Fv at a stable level and had a smaller regression slope (ß1), whereas the susceptible cultivars showed a very dramatic decrease in Fv. The CF method gave results that correlated with the VEN results. The strong relationship between chilling tolerance determined via visual and florescence techniques supports the use of CF in selecting resistant spring frost selections in a breeding program. The use of CF will allow the breeder not only to select for spring frost-resistant selections independently of environmental changes, but also to select frost resistant seedlings prior to planting in the field.

STORABILITY OF FARM-STORED HULLESS OATS IN MANITOBA

1979 ◽  
Vol 59 (4) ◽  
pp. 949-957 ◽  
Author(s):  
R. N. SINHA ◽  
H. A. H. WALLACE ◽  
J. T. MILLS ◽  
R. I. H. McKENZIE
Keyword(s):  
Storage Temperature ◽  
First Year ◽  
Aspergillus Versicolor ◽  
Cryptolestes Ferrugineus ◽  
Moisture Contents ◽  
Grain Moisture ◽  
Avena Nuda ◽  
Low Levels ◽  
Second Year ◽  
Temperature Levels

Storability of properly harvested, mature hulless oats (Avena nuda L. ’Terra’) of 9.7–10.5% moisture content was determined from a 24-mo field trial of two 11.5-t lots of oats stored in two wooden farm bins in Manitoba during 1976–78. Oats were stored safely with very low levels of infestation by preharvest fungi, mites and insects and negligible quality loss for up to about 20 mo. Afterwards the grain moisture contents at the bottom layers of both bins rose from about 10 to 13–16%; this led to heavy floor level infestations by the fungi Penicillium and Aspergillus versicolor, and mites Caloglyphus berlesei and Tarsonemus granarius. Fat acidity values rose from the first year levels of about 30 to 50–78 mg KOH/100 g of dry grain. Populations of Cryptolestes ferrugineus and Tribolium castaneum, introduced to one bin, did poorly during the first summer and failed to survive during the second year. A third beetle, Oryzaephilus surinamensis, continued to multiply in small numbers after 22 mo. Low storage temperature levels during most of the years seemed to have affected adversely the multiplication of insects and to a lesser extent mites in farm-stored oats.

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