scholarly journals Rapid Detection of Bloodstream Pathogens in Oncologic Patients with a FilmArray Multiplex PCR Assay: a Comparison with Culture Methods

2018 ◽  
Vol 67 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Maria Szymankiewicz ◽  
Beata Nakonowska
Keyword(s):  
Blood Culture ◽  
Multiplex Pcr ◽  
Accurate Method ◽  
Rapid Identification ◽  
Blood Cultures ◽  
Pcr Assay ◽  
Culture Methods ◽  
Multiplex Pcr Assay ◽  
Oncologic Patients ◽  
Culture Identification

The results of the FilmArray® Blood Culture Identification Panel (BCID) (BioFire Diagnostics) and the culture with susceptibility testing of 70 positive blood cultures from oncologic patients were compared. The multiplex PCR assay (BCID) identified 81 of the 83 isolates (97.6%), covered by the panel. The panel produced results in significantly shorter time than standard identification methods, when counted from receiving positive blood cultures bottles to the final results. It is an accurate method for the rapid identification of pathogens and resistance genes from blood culture in oncologic patients.

scholarly journals Evaluation of the Clinical Impact of the Biofire Filmarray® Rapid Multiplex PCR Assay in Blood Culture Identification Combined with Antimicrobial Stewardship Intervention

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S627-S627
Author(s):  
Gabriela M Andujar-Vazquez ◽  
Bradley Gardiner ◽  
Francis Magro ◽  
Kirthana R Beaulac ◽  
Shira Doron ◽  
...  
Keyword(s):  
Blood Culture ◽  
Multiplex Pcr ◽  
Antimicrobial Stewardship ◽  
Clinical Impact ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Culture Identification

scholarly journals Impact of a Multiplex PCR Assay for Bloodstream Infections With and Without Antimicrobial Stewardship Intervention at a Cancer Hospital

2018 ◽  
Vol 5 (10) ◽  
Author(s):  
Brian A Buss ◽  
Timothy J Baures ◽  
Minkyoung Yoo ◽  
Kimberly E Hanson ◽  
Donald P Alexander ◽  
...  
Keyword(s):  
Blood Culture ◽  
Multiplex Pcr ◽  
Antimicrobial Stewardship ◽  
Antimicrobial Therapy ◽  
Bloodstream Infections ◽  
Pcr Assay ◽  
Cancer Hospital ◽  
Multiplex Pcr Assay ◽  
Culture Identification ◽  
Reduced Time

Abstract Implementation of Biofire FilmArray Blood Culture Identification Multiplex PCR panel (BCID) at a cancer hospital was associated with reduced time to appropriate antimicrobial therapy. Additional reductions were not observed when BCID was coupled with antimicrobial stewardship intervention.

scholarly journals Multiplex PCR assay for the identification of eight Anopheles species belonging to the Hyrcanus, Barbirostris and Lindesayi groups

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Woo Jun Bang ◽  
Heung Chul Kim ◽  
Jihun Ryu ◽  
Hyeon Seung Lee ◽  
So Youn Lee ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Molecular Identification ◽  
Malaria Infection ◽  
Diagnostic System ◽  
Accurate Method ◽  
Its2 Region ◽  
Infection Rates ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
The Republic

Abstract Background Genus Anopheles mosquitoes are the primary vectors of human malaria, which is a serious threat to public health worldwide. To reduce the spread of malaria and identify the malaria infection rates in mosquitoes, accurate species identification is needed. Malaria re-emerged in 1993 in the Republic of Korea (ROK), with numbers peaking in 2004 before decreasing to current levels. Eight Anopheles species (Anopheles sinensis, Anopheles pullus, Anopheles belenrae, Anopheles lesteri, Anopheles kleini, Anopheles sineroides, Anopheles koreicus, Anopheles lindesayi) are distributed throughout Korea. Members of the Anopheles Hyrcanus group currently cannot be identified morphologically. The other species of Anopheles can be identified morphologically, except when specimens are damaged in traps. The purpose of this study was to develop a rapid and accurate method for simultaneous molecular identification of the eight Anopheles species present in the ROK. Methods Anopheles spp. used in this study were collected near/in the demilitarized zone in ROK, where most malaria cases are reported. DNA from 165 of the Anopheles specimens was used to develop a multiplex PCR assay. The internal transcribed spacer 2 (ITS2) region of each species was sequenced and analysed for molecular identification. Results DNA from a total of 165 Anopheles specimens was identified to species using a multiplex diagnostic system. These included: 20 An. sinensis, 21 An. koreicus, 17 An. lindesayi, 25 An. kleini, 11 An. lesteri, 22 An. sineroides, 23 An. belenrae, and 26 An. pullus. Each species was clearly distinguished by electrophoresis as follows: 1,112 bp for An. sinensis; 925 bp for An. koreicus; 650 bp for An. lindesayi; 527 bp for An. kleini; 436 bp for An. lesteri; 315 bp for An. sineroides; 260 bp for An. belenrae; and, 157 bp for An. pullus. Conclusion A multiplex PCR assay was developed to identify Anopheles spp. distributed in ROK. This method can be used to accurately identify Anopheles species that are difficult to identify morphologically to determine species distributions and malaria infection rates.

scholarly journals Multiplex PCR Assay for Identifi cation and Differentiation of Campylobacter jejuni and Campylobacter coli Isolates

Folia Medica ◽  
2016 ◽  
Vol 58 (2) ◽  
pp. 95-100 ◽  
Author(s):  
Maria R. Pavlova ◽  
Elina G. Dobreva ◽  
Katucha I. Ivanova ◽  
Galina D. Asseva ◽  
Ivan N. Ivanov ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Campylobacter Jejuni ◽  
Accurate Method ◽  
Clinical Isolates ◽  
Campylobacter Coli ◽  
Pcr Assay ◽  
Biochemical Tests ◽  
Multiplex Pcr Assay ◽  
Hydrolysis Of ◽  
Campylobacter Spp

AbstractIntroduction: Campylobacter spp. are important causative agents of gastrointestinal infections in humans. The most frequently isolated strains of this bacterial genus are Campylobacter jejuni and Campylobacter coli. To date, genetic methods for bacterial identification have not been used in Bulgaria. We optimized the multiplex PSR assay to identify Campylobacter spp. and differentiate C. jejuni from C. coli in clinical isolates. We also compared this method with the routinely used biochemical methods.Aim: To identify Campylobacter spp. and discriminate C. coli from C. jejuni in clinical isolates using multiplex PCR assay.Materials and methods: Between February 2014 and January 2015 we studied 93 stool samples taken from patients with diarrheal syndrome and identified 40 species of Campylobacter spp. in them. The clinical material was cultured in microaerophilic atmosphere, the isolated strains being biochemically diff erentiated (hydrolysis of sodium hippurate for C. jejuni, and hydrolysis of indoxyl acetate for C. coli). DNA was isolated from the strains using QiaAmp MiniKit (QIAGEN, Germany). Twenty strains were tested with multiplex PCR for the presence of these genes: cadF, characteristic for Campylobacter spp., hipO for C. jejuni and asp for C. coli.Results and discussion: The biochemical tests identified 16 strains of C. jejuni, 3 strains of C. coli, and 1 strain of C. upsaliensis. After the multiplex PCR assay the capillary gel electrophoresis confirmed 16 strains of C. jejuni, 2 strains of C. coli and 2 strains of Campylobacter spp. - because of the presence of the gene cadF. C. jejuni has the gene hipO, and it is possible that this gene may not be expressed in the biochemical differentiation yielding a negative reaction as a result. In comparison, we can conclude that the genetic differentiation is a more accurate method than the biochemical tests.Conclusion: The multiplex PCR assay is a fast, accurate method for identifi cation of Campylobacter spp. which makes it quite necessary in the clinical diagnostic practice.

scholarly journals Rapid identification of Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii with a multiplex PCR assay

2014 ◽  
Vol 63 (9) ◽  
pp. 1154-1159 ◽  
Author(s):  
Te-Li Chen ◽  
Yi-Tzu Lee ◽  
Shu-Chen Kuo ◽  
Su-Pen Yang ◽  
Chang-Phone Fung ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multiplex Pcr ◽  
Acinetobacter Calcoaceticus ◽  
Rapid Identification ◽  
23S Rrna ◽  
Pcr Assay ◽  
Complex Species ◽  
Multiplex Pcr Assay ◽  
Acinetobacter Pittii ◽  
Reference Strains

Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii are clinically relevant members of the Acinetobacter calcoaceticus–A. baumannii (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. In this study, we investigated the use of a multiplex PCR-based assay designed to detect internal fragments of the 16S–23S rRNA intergenic region and the gyrB and recA genes. The assay was capable of differentiating A. baumannii, A. nosocomialis and A. pittii in a reliable manner. In 23 different reference strains and 89 clinical isolates of Acinetobacter species, the assay accurately identified clinically relevant Acb complex species except those ‘between 1 and 3’ or ‘close to 13TU’. None of the non-Acb complex species was misidentified. In an analysis of 1034 positive blood cultures, the assay had a sensitivity of 92.4 % and specificity of 98.2 % for Acb complex identification. Our results show that a single multiplex PCR assay can reliably differentiate clinically relevant Acb complex species. Thus, this method may be used to better understand the clinical differences between infections caused by these species.

scholarly journals Rapid Detection of Methicillin-Resistant Staphylococci from Blood Culture Bottles by Using a Multiplex PCR Assay

2002 ◽  
Vol 40 (8) ◽  
pp. 2786-2790 ◽  
Author(s):  
L. Louie ◽  
J. Goodfellow ◽  
P. Mathieu ◽  
A. Glatt ◽  
M. Louie ◽  
...  
Keyword(s):  
Blood Culture ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Methicillin Resistant ◽  
Multiplex Pcr Assay

scholarly journals Accurate and Rapid Identification of Longan Arillus and Litchi Semen by a Multiplex PCR Assay

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 948
Author(s):  
Wook Jin Kim ◽  
Sungyu Yang ◽  
Goya Choi ◽  
Inkyu Park ◽  
Pureum Noh ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Markers ◽  
Herbal Medicines ◽  
High Specificity ◽  
Single Species ◽  
Rapid Identification ◽  
Genetic Identification ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Specificity And Sensitivity

Dimocarpus longan, Litchi chinensis, and Nephelium lappaceum are commercially valuable subtropical and tropical fruits of the Sapindaceae family. Arillus and seeds of the three species have very similar morphologies; however, the arillus of D. longan is used as the herbal medicine Longan Arillus and seeds of L. chinensis are used as Litchi Semen in Korean and Chinese pharmacopoeias. The adulteration of herbal medicines with inauthentic species, including the use of Aril and seed fractions acquired from a single species for two herbal medicines (e.g., Longan Arillus and Litchi Semen), is often driven by economic motives. DNA markers are a tool for the detection of adulterants in commercial products. To establish rapid and reliable assays for the genetic identification of authentic Longan Arillus and Litchi Semen, we developed DNA markers with high specificity and sensitivity based on internal transcribed spacer (ITS) sequences. The newly developed DNA markers and multiplex PCR assay may contribute to efforts to protect against adulteration, quality control, and the standardization of herbal medicines.

Multiplex PCR assay for rapid identification of three endangered snake species of India

2009 ◽  
Vol 10 (6) ◽  
pp. 1861-1864 ◽  
Author(s):  
Bhawna Dubey ◽  
P. R. Meganathan ◽  
Ikramul Haque
Keyword(s):  
Multiplex Pcr ◽  
Rapid Identification ◽  
Pcr Assay ◽  
Snake Species ◽  
Multiplex Pcr Assay

scholarly journals A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types

2019 ◽  
Vol 164 (3) ◽  
pp. 819-830 ◽  
Author(s):  
Yannick Born ◽  
Leandra E. Knecht ◽  
Mirjam Eigenmann ◽  
Michel Bolliger ◽  
Jochen Klumpp ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Capsid Protein ◽  
Major Capsid Protein ◽  
Rapid Identification ◽  
Pcr Assay ◽  
Multiplex Pcr Assay
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