scholarly journals Birth of a healthy infant after bone marrow-derived cell therapy

2021 ◽  
Vol 48 (3) ◽  
pp. 268-272
Nayana H Patel ◽  
Yuvraj D Jadeja ◽  
Niket H Patel ◽  
Molina N Patel ◽  
Harsha K Bhadarka ◽  

Bone marrow-derived cell (BMDC) therapy has numerous applications as potential biological cells for use in regenerative medicine. Here, we present an original case of endometrial atrophy associated with genital tuberculosis in a woman who achieved a live birth with BMDC. This 27-year-old woman came to our center with endometrial atrophy and primary infertility. She had a past history of genital tuberculosis and amenorrhea. Her husband’s semen quality was normal. The patient was counseled for hysteroscopy due to thin endometrium and advised in vitro fertilization (IVF) with donor eggs in lieu of poor ovarian reserve. Several attempts of IVF with hormone replacement therapy (HRT) were made, but the desired thickness of the endometrium was not achieved. Uterine artery injection of BMDC through interventional radiology was given, followed by HRT for three months, which resulted in improved endometrium. This was subsequently followed by IVF with donor egg. The treatment resulted in the conception and delivery of a 3.1-kg baby boy through lower segment caesarean section with no antenatal, intranatal or postnatal complications. Recently, there has been massive interest in stem cells as a novel treatment method for regenerative medicine, and more specifically for the regeneration of human endometrium disorders like Asherman syndrome and thin endometrium, which was the reason behind using this strategy for treatment.

2017 ◽  
Vol 8 (3) ◽  
pp. 101-105
Kundavi Shankar ◽  
Shipra Nigam ◽  
Thankam R Varma

ABSTRACT Introduction One of the most challenging problems in in vitro fertilization (IVF) is patient with thin endometrium. The objective of the study was to ascertain whether daily human chorionic gonadotropin (hCG) for 7 days with estrogen in hormone replacement frozen embryo transfer (FET) cycles during follicular phase can increase the endometrial thickness (ET) and reduce the cancellation of cycles. Materials and methods Twenty-five infertile patients with resistant thin endometrium who had antagonist protocol and planned for frozen embryo replacement were recruited. These patients had prior attempts to thicken their endometrium which had failed. All the patients received estrogen daily from D2/3 of cycle. On day 8 or 9 of estrogen administration, 200 IU of hCG was given daily for 7 days. After 7 days on hCG priming (D14/15), ET was measured and progesterone was started accordingly. Identification of an intrauterine gestational sac with fetal heart beat by transvaginal ultrasonography constituted clinical pregnancy. Results Mean ET increased significantly from 5.84 to 7.61 mm (p < 0.01). About 72% of patients had more than 20% improvement in their ET after hCG priming. About 76% achieved an ET more than 7 mm. Overall, 50% became pregnant. The ongoing pregnancy rate was 40%. Conclusion A total of 200 IU hCG endometrial priming for 7 days in the proliferative phase of hormone replacement cycles for FET is a highly promising approach to thicken thin endometrium with failed prior attempts. How to cite this article Nigam S, Shankar K, Varma TR. Role of Low-dose Human Chorionic Gonadotropin in Follicular Phase for Thin Endometrium in Frozen Embryo Replacement Cycles in in vitro Fertilization/Intracytoplasmic Sperm Injection Patients: A Pilot Study. Int J Infertil Fetal Med 2017;8(3):101-105.

2015 ◽  
Vol 6 (2) ◽  
pp. 73-83 ◽  
Prabhjot Singh Hans ◽  
Mohan Lal Swarankar ◽  
Swati Garg ◽  
Manisha Chowdhary ◽  
Karnika Tiwari

ABSTRACT Aim To assess ovarian reserve, in infertile women with genital tuberculosis, planning to undergo in vitro fertilization (IVF) and to compare it with infertile women without genital tuberculosis, planning to undergo IVF. Materials and methods The study group consisted of 100 women with genital tuberculosis and the control group of 100 women who had no present or past history of tuberculosis. A diagnosis of genital tuberculosis was made based either on the results of tests performed from an endometrial aspiration sample or on histopathologic, hysterosalpingography, hysteroscopy, or laparoscopy findings. Basal ovarian reserve studies included measuring serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and E2 on day 3 of a natural cycle. On the same day, the participants underwent a transvaginal ultrasound examination by means of a two-dimensional 5.0 MHz probe fitted to a Toshiba Famio 5. Ovarian volume and number of antral follicles were estimated for each ovary on the same day of hormonal assessment. Control participants underwent the same tests on day 2 or 3 of their menstrual cycle. Unpaired or independent t-test and Chi-square test were used for statistical analysis. Results The present study highlights that women with genital tuberculosis have poor ovarian reserve in comparison to women of similar age without tuberculosis. Conclusion It can be concluded that there is no single absolute method of assessing ovarian reserve, but a combination of methods can closely predict the outcome of IVF cycles in women with genital tuberculosis. How to cite this article Hans PS, Swarankar ML, Garg S, Chowdhary M, Tiwari K. Effect of Tuberculosis on Ovarian Reserve of Patients undergoing IVF. Int J Infertil Fetal Med 2015;6(2):73-83.

1993 ◽  
Vol 8 (2) ◽  
pp. 253-257 ◽  
C.J. De Jonge ◽  
S.M. Tarchala ◽  
R.G. Rawlins ◽  
Z. Binor ◽  
E. Radwanska

Mayuko Kano ◽  
Hidetaka Suga ◽  
Hiroshi Arima

Abstract The hypothalamus and pituitary have been identified to play essential roles in maintaining homeostasis. Various diseases can disrupt the functions of these systems, which can often result in serious lifelong symptoms. The current treatment for hypopituitarism involves hormone replacement therapy. However, exogenous drug administration cannot mimic the physiological changes that are a result of hormone requirements. Therefore, patients are at a high risk of severe hormone deficiency, including adrenal crisis. Pluripotent stem cells (PSCs) self-proliferate and differentiate into all types of cells. The generation of endocrine tissues from PSCs has been considered as another new treatment for hypopituitarism. Our colleagues established a three-dimensional culture method for embryonic stem cells (ESCs). In this culture, the ESC-derived aggregates exhibit self-organization and spontaneous formation of highly ordered patterning. Recent results have shown that strict removal of exogenous patterning factors during early differentiation efficiently induces rostral hypothalamic progenitors from mouse ESCs. These hypothalamic progenitors generate vasopressinergic neurons, which release neuropeptides upon exogenous stimulation. Subsequently, we reported adenohypophysis tissue self-formation in three-dimensional cultures of mouse ESCs. The ESCs were found to differentiate into both non-neural oral ectoderm and hypothalamic neuroectoderm in adjacent layers. Interactions between the two tissues appear to be critically important for in vitro induction of a Rathke's pouch-like developing embryo. Various endocrine cells were differentiated from non-neural ectoderm. The induced corticotrophs efficiently secreted adrenocorticotropic hormone when engrafted in vivo, which rescued hypopituitary hosts. For future regenerative medicine, generation of hypothalamic and pituitary tissues from human PSCs is necessary. We and other groups succeeded in establishing a differentiation method with the use of human PSCs. Researchers could use these methods for models of human diseases to elucidate disease pathology or screen potential therapeutics.

2013 ◽  
Vol 25 (1) ◽  
pp. 301
P. L. Jensen ◽  
M. L. Groendahl ◽  
H. C. Beck ◽  
J. Petersen ◽  
L. Stroebech ◽  

Human embryonic stem cells (hESC) are derived from the human blastocyst and possess the potential to differentiate into any cell type present in the adult human body. Human ESC are considered to have great potential in regenerative medicine for the future treatment of severe diseases and conditions such as Parkinson’s disease, diabetes, and spinal cord injury. One of today’s challenges in regenerative medicine is to define proper culture conditions for hESC. The natural milieu in the blastocyst may provide clues on how to improve culture conditions, and the aim of the present study was to determine the proteome of the blastocoel fluid and the remaining cells of bovine blastocysts. Bovine blastocysts were produced by in vitro fertilization of oocytes retrieved from slaughterhouse ovaries. The blastocoel from 195 blastocysts (1–8 nL per blastocyst) were isolated by micromanipulation and analysed by nano-HPLC tandem mass spectrometry along with the remaining cells of the blastocyst. Searching the mass spectrometry data against a combined bovine database (SwissProt/TrEMBL), we identified 263 proteins in the blastocoel fluid and 1606 proteins in the cellular compartment of the blastocyst. A Venn diagram showed 124 proteins in overlap between the two compartments of the blastocyst. Several heat shock proteins and specific antioxidants were identified in both the blastocoel and cell material. A selection of proteins identified in the blastocoel fluid is to be tested on hESC in cell culture experiments, with proliferation of undifferentiated cells as the primary endpoint. The results from this study provide new knowledge about early mammalian preimplantation development, and the data can be used in the continued pursue of improving culture conditions for hESC, which further facilitates the clinical application of these cells.

2014 ◽  
Vol 24 (3) ◽  
pp. 384-393 ◽  
Aus Tariq Ali

AbstractEndometrial cancer is the most common malignancy of women in developed countries, and its incidence is 10 times higher than in developing countries. Endometrial cancer is most common in the sixth and the seventh decades of life; thus, postmenopausal women have a higher risk of developing the disease compared with premenopausal women. The increased incidence and prevalence of endometrial cancer can be explained by the increase in life expectancy, increased caloric intake, increased obesity rates, and other changes in lifestyle and reproductive factors. Among the reproductive factors, the risk of endometrial cancer is positively correlated with a younger age at menarche and late age at menopause, infertility, null parity, age of the first child, and long-term use of unopposed estrogens for hormone replacement therapy. Protection against endometrial cancer has been detected with increase parity, the use of combined oral contraceptives, and increased age of women at last delivery. The relationship between endometrial cancer risk and miscarriage, abortion, ovulation induction drugs and in vitro fertilization is still controversial.

2013 ◽  
Vol 99 (6) ◽  
pp. 1565-1572.e2 ◽  
Elisa Vellani ◽  
Alessandro Colasante ◽  
Luciana Mamazza ◽  
Maria Giulia Minasi ◽  
Ermanno Greco ◽  

2016 ◽  
Vol 2016 ◽  
pp. 1-18 ◽  
Subhash C. Juneja ◽  
Sowmya Viswanathan ◽  
Milan Ganguly ◽  
Christian Veillette

The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon’s skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting. This represents an economical source and convenient source of MSCs from bone marrow for use in regenerative medicine. Also, we presented the detailed protocol and showed that the MSCs derived from such bone marrow specimens exhibited MSCs characteristics and generated micromass cartilages, the recipe for regenerative medicine for osteoarthritis. The protocols we presented can be used as standard operating procedures (SOPs) by researchers and clinicians.

1988 ◽  
Vol 139 (5) ◽  
pp. 1138-1139 ◽  
K.L. Harrison ◽  
V.J. Callan ◽  
J.F. Hennessey

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