Cysteine improves boar sperm quality via glutathione biosynthesis during the liquid storage

Lipopolysaccharide (LPS) released from Gram-negative bacteria binds to toll-like receptor 4 (TLR4) and induces boar sperm apoptosis. Similarly, polyhistidine (pHis), a TLR4 agonist, can also bind to TLR4. We hypothesized that pHis could inhibit LPS-induced sperm apoptosis by competitively binding to TLR4 to then improve sperm quality. Therefore, the objective of this study was to examine whether pHis can inhibit LPS-induced sperm apoptosis and affect sperm quality. The results showed that the concentrations of bacterial colonies were significantly increased from 36 to 120 h under liquid storage conditions (p < 0.05); however, concentrations of LPS in boar semen showed a relatively constant trend (4.98 ± 1.55 EU/mL) following 120 h storage. The addition of 100 μg/mL pHis in the BTS extender significantly improved boar sperm motility and viability at 37 °C, and it significantly (p < 0.05) inhibited boar sperm apoptosis under liquid storage (17 °C) and at 37 °C incubation conditions. The co-treatment of LPS and pHis further confirmed that pHis played its role in inhibiting LPS-induced sperm apoptosis. In conclusion, our preliminary findings provide reasonable evidence that pHis could act as an inhibitor of LPS-induced apoptosis in boar sperm stored for longer periods of time. pHis might inhibit LPS-induced sperm apoptosis by competitively binding to TLR4. Nevertheless, further mechanistic studies are awaited to fully elucidate its potential implication in inhibiting LSP-induced apoptosis.

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Effects of Isatis root polysaccharide on boar sperm quality during liquid storage and in vitro fertilization

Animal Reproduction Science ◽

10.1016/j.anireprosci.2019.106178 ◽

2019 ◽

Vol 210 ◽

pp. 106178 ◽

Cited By ~ 1

Author(s):

Zhiqiang Ren ◽

Weike Shaoyong ◽

Qian Li ◽

Lu Ma ◽

Junying Xiao ◽

...

Keyword(s):

In Vitro Fertilization ◽

Sperm Quality ◽

Vitro Fertilization ◽

Liquid Storage ◽

Boar Sperm

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Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C

Animal Science Journal ◽

10.1111/asj.13281 ◽

2019 ◽

Vol 90 (9) ◽

pp. 1161-1169 ◽

Cited By ~ 1

Author(s):

Tian‐Yu Feng ◽

Fa Ren ◽

Qian Fang ◽

Gui‐Chao Dai ◽

Yu Li ◽

...

Keyword(s):

Sperm Quality ◽

Bacterial Composition ◽

Liquid Storage ◽

Boar Sperm

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Effects of Bovine Serum Albumin on Boar Sperm Quality During Liquid Storage at 17°C

Reproduction in Domestic Animals ◽

10.1111/rda.12481 ◽

2015 ◽

Vol 50 (2) ◽

pp. 263-269 ◽

Cited By ~ 20

Author(s):

X-G Zhang ◽

G-J Yan ◽

J-Y Hong ◽

Z-Z Su ◽

G-S Yang ◽

...

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Sperm Quality ◽

Liquid Storage ◽

Boar Sperm

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Effect of liquid storage on membrane integrity and mitochondrial activity: a new diagnostic method of evaluating boar sperm quality

Journal of Animal and Feed Sciences ◽

10.22358/jafs/66625/2008 ◽

2008 ◽

Vol 17 (3) ◽

pp. 372-380 ◽

Cited By ~ 8

Author(s):

M. Trzcińska ◽

M. Bryła ◽

Z. Smorąg

Keyword(s):

Diagnostic Method ◽

Sperm Quality ◽

Membrane Integrity ◽

Mitochondrial Activity ◽

Liquid Storage ◽

Boar Sperm

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Effect of L-carnitine on sperm quality during liquid storage of boar semen

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.19.0455 ◽

2020 ◽

Vol 33 (11) ◽

pp. 1763-1769

Author(s):

Kang Yang ◽

Na Wang ◽

Hai-Tao Guo ◽

Jing-Ran Wang ◽

Huan-Huan Sun ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Quality ◽

Membrane Integrity ◽

Quality Parameters ◽

Atp Content ◽

Developmental Potential ◽

Positive Effects ◽

Liquid Storage ◽

Boar Sperm

Objective: This study was conducted to investigate the effect of L-carnitine on the pig semen characteristics during storage.Methods: Spermatozoa samples were examined for spermatozoa quality and then randomly divided into 5 groups: 0 (control), 12.5, 25, 50, and 100 mM L-carnitine. Sperm motility, plasma membrane integrity and antioxidant parameters (total reactive oxygen species, total antioxidant capacity, and malondialdehyde) were evaluated after 0, 3, 5, and 10 day cooledstorage at 17°C. Moreover, ATP content, mitochondria activity as well as sperm-binding and in vitro fertilizing ability of preserved boar sperm were also investigated.Results: Supplementation with 50 mM L-carnitine could effectively maintain boar sperm quality parameters such as sperm motility and membrane integrity. Besides, we found that L-carnitine had positive effects on boar sperm quality mainly through improving antioxidant capacities and enhancing ATP content and mitochondria activity. Interestingly, by assessing the effect of L-carnitine on sperm fertility and developmental potential, we discovered that the extender containing L-carnitine could improve sperm quality and increase the number of sperms bounding to zona pellucida, without improving in vitro fertility and development potential.Conclusion: These findings suggested that the proper addition of L-carnitine to the semen extender improved boar sperm quality during liquid storage at 17°C.

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