scholarly journals Genetic Relationships of Cattle Breeds Assessed by PCR-RFLP of the Bovine Mitochondrial DNA D-loop Region

2005 ◽  
Vol 18 (10) ◽  
pp. 1368-1374 ◽  
Author(s):  
Du Hak Yoon ◽  
Hak Kyo Lee ◽  
Sung Jung Oh ◽  
Ki Chang Hong ◽  
Gwang Joo Jeon ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Relationships ◽  
Cattle Breeds ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop

Polymorphisms of genes required for methionine synthesis and DNA methylation influence mitochondrial DNA methylation

Epigenomics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1003-1012
Author(s):  
Andrea Stoccoro ◽  
Pierpaola Tannorella ◽  
Lucia Migliore ◽  
Fabio Coppedè
Keyword(s):  
Dna Methylation ◽  
Mitochondrial Dna ◽  
Exploratory Study ◽  
High Resolution Melting ◽  
Late Onset ◽  
Loop Region ◽  
Pcr Rflp ◽  
D Loop ◽  
One Carbon Metabolism ◽  
Methionine Synthesis

Aim: Impaired methylation of the mitochondrial DNA and particularly in the regulatory displacement loop (D-loop) region, is increasingly observed in patients with neurodegenerative disorders. The present study aims to investigate if common polymorphisms of genes required for one-carbon metabolism ( MTHFR, MTRR, MTR and RFC-1) and DNA methylation reactions ( DNMT1, DNMT3A and DNMT3B) influence D-loop methylation levels. Materials & methods: D-loop methylation data were available from 133 late-onset Alzheimer’s disease patients and 130 matched controls. Genotyping was performed with PCR-RFLP or high resolution melting techniques. Results: Both MTRR 66A > G and DNMT3A -448A > G polymorphisms were significantly associated with D-loop methylation levels. Conclusion: This exploratory study suggests that MTRR and DNMT3A polymorphisms influence mitochondrial DNA methylation; further research is required to better address this issue.

scholarly journals Genetic relationships between local Brazilian goat breeds based on mtDNA D-loop region similarity

2020 ◽  
Vol 17 (4) ◽  
pp. e0407 ◽  
Author(s):  
Núbia M. V. Silva ◽  
Edgard C. Pimenta-Filho ◽  
Janaina K. G. Arandas ◽  
Rosália B. N. Medeiros ◽  
Aderbal Cavalcante-Neto ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Population Structure ◽  
Genetic Relationships ◽  
Hypervariable Region ◽  
Genetic Composition ◽  
Mitochondrial Lineage ◽  
Breeding Programs ◽  
Loop Region ◽  
Population Structure Analysis ◽  
D Loop

Aim of study: Our objective was to investigate the mitochondrial DNA of local Brazilian goats to gain insights into the genetic composition of this precious genetic resource.Area of study: The study was developed in BrazilMaterial and methods: We analyzed a hypervariable region of the mitochondrial DNA of 83 goats belonging to four local Brazilian breeds, including Canindé (CAN-RN), Moxotó (MOX-CE), Marota (MAR-PI) and Azul (AZU-PE) as well as of exotic breeds raised in different states of the Federation. Sequences related to local Brazilian goats showed a dispersed distribution throughout the median-joining network, and clustering with sequences of exotic breeds occurred in some haplotypes. The obtained sequences were analyzed and compared with different haplogroups (A, B1, B2, C, D, F, and G) available on GenBank.Main results: The local Brazilian goat breeds showed significant diversity, with 16 (0.8240) haplotypes. Population structure analysis revealed substantial differences among breeds (p < 0.05). Mitochondrial lineage A was observed in Brazilian goats. Phylogeny showed European goats as the dominant stock for Brazilian goats, but there weare some haplotypes within haplogroup A, clustering with African and Asian haplotypes.Research highlights: These results could be suitable for creating a strategic conservation program, potentially benefitting future breeding programs.

scholarly journals The genetic profiles and maternal origin of local sheep breeds on Java Island (Indonesia) based on complete mitochondrial DNA D-loop sequences

2020 ◽  
Vol 13 (12) ◽  
pp. 2625-2634
Author(s):  
Alek Ibrahim ◽  
I Gede Suparta Budisatria ◽  
Rini Widayanti ◽  
Wayan Tunas Artama
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Relationships ◽  
Haplotype Diversity ◽  
Maternal Origin ◽  
Loop Analysis ◽  
High Genetic Diversity ◽  
Sheep Breeds ◽  
Loop Region ◽  
D Loop ◽  
Genetic Profiles

Background and Aim: Java Island is one of the islands in Indonesia which has local sheep breeds with specific characteristics and native development geography in certain regions. This study aimed to determine the genetic profiles and maternal origin of six local sheep breeds on Java Island. Materials and Methods: This study was conducted by identifying the profiles of complete mitochondrial DNA (mtDNA) displacement loop (D-loop) region sequences on a total of 22 individual in six local sheep breeds on Java Island, including Javanese thin-tailed (JTT), Javanese Fat-Tailed (JFT), Batur (BTR), Wonosobo (WSB), Garut (GRT), and Priangan (PRG) sheep. The D-loop region was amplified using specific primers, and the polymerase chain reaction (PCR) was performed. The PCR products were purified and sequenced. Results: The mtDNA D-loop analysis identified 21 haplotypes in the analyzed 22 animals with 123 polymorphic sites (V) consisting of 60 singleton variable sites (S) and 63 parsimony informative sites (P). Within all breeds tested, the haplotype diversity, the average number of pairwise differences (K), and nucleotide diversity (Pi) were 0.99567, 25.36364, and 0.02153, respectively. The genetic distance (D) within groups and between groups was 0.001-0.006 and 0.004-0.036, respectively. The phylogeny resulted in the presence of two haplogroups (Hap), which are 5 Hap A and 16 Hap B. All JTT, JFT, BTR, and WSB breeds were in the same cluster in Hap B, whereas GRT and PRG breeds were in clusters in both Hap A and Hap B. Conclusion: The high genetic diversity in six local sheep breeds on Java Island suggests that they originated from different genetic sources. JTT sheep have closer genetic relationships to JFT, BTR, and WSB sheep, and they are close to European sheep, whereas GRT sheep have closer genetic relationships to PRG sheep. Both are closer to Asian sheep than to European sheep.

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