scholarly journals Healing Effects of Iranian Dwarf Elder on Full-thickness Epidermal Thermal Injury in Wistar Rats

2022 ◽  
Vol 19 (4) ◽  
Author(s):  
Hosein Mohamadi haftador ◽  
Parisa Ramhormozi ◽  
Mitra Yousefpour ◽  
Ali Sobhanizadeh ◽  
Laya Ghahari

Background: Wound healing is a physiologic process that cells and tissues react when the skin is exposed to thermal injury. The inflammatory process has ‎essential effects on wound healing that includes coordination between immunological and biological responses. Chemical and herbal medicines are locally or systemically administrated to help wound healing. Herbal medicines have become more prevalent in recent years due to fewer side effects. Dwarf elder is used to treating inflammation and has been reported to possess antibacterial and antioxidant activities. This plant is also effective against burns, wounds, eczema, and infection. Objectives: In this study, we evaluated the topical application of dwarf elder on full-thickness epidermal thermal wounds in Wistar rats. Methods: Thirty adult male Wistar rats of about 300 g were used. The rats were randomly divided into three groups: Control group, vehicle group treated with an ointment base, and treatment group treated with elder ointment 10%. The animals were anesthetized and were created burn wounds with an area of 1 cm2 using the Meyer method. After 21 days, the wounds were photographed with a Canon camera. The wound area and healing degree were calculated. Slides were stained with H&E and examined by light microscopy and GraphPad Prism software. Data were analyzed by SPSS software using ANOVA and Tukey’s test. The significance level was considered at P < 0.05. Results: The wound area on the 21st day was significantly lower in the treatment group than in the control and vehicle groups. The epithelium thickness (μm) was significantly higher in the treatment group than in the control and vehicle groups. The blood vessels were significantly higher in the treatment group than in the other groups. Conclusions: The study showed that elder extract could be an effective remedy to repair and heal thermal injury and burn wounds.

2019 ◽  
Vol 1 (1) ◽  
pp. 15
Author(s):  
Afryla Femilian ◽  
Dewi Agustina ◽  
Goeno Subagyo

Indonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These  ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3rd, 7th and 12th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was <0.05. In the treatment group on day 0 compared to the treatment group on the 3rd, 7th, 12th day, the results were p<0.05. In the treatment group on the 3rd day compared to the 7th and 12th days the results were p>0.05. The treatment group on the 7 day compared to the treatment group on the 12 day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p<0.05. The control group on the 3rd day was compared with the 7th day. The 12th also had a result of p<0.05. The comparison between the 7 day control groups showed p of >0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats.


2021 ◽  
Vol 18 (9) ◽  
pp. 1909-1912
Author(s):  
Liang Wang ◽  
Li Yang ◽  
Gang Yuan ◽  
Long-Jiao Hu

Purpose: To evaluate the healing effect of Platycodon grandiflorus (Jacq.). extract (PGE) on experimental burn wounds in rats. Methods: Rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and PGE-treated group. PGE, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. External observation of wound area contraction and histological analysis of wound tissues was performed respectively. The effect of PGE on matrix metalloproteases (MMPs), vascular endothelial growth factor (VEGF) and Type-III Collagen proteins of wound tissue in rats were analysed by Western blot. Results: After 10 days of topical treatment with PGE, PGE-treated group showed faster reduction in wound area when compared with control groups (p < 0.01). Matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), VEGF and type-III collagen expressions in the wound tissue increased significantly (p < 0.05) when compared with the control burn wounds. Histological results showed an overall early recovery and regeneration in PGE-treated group when compared with control group. Conclusion: PGE possesses a significant wound-healing activity in full-thickness burn wounds in rats. Therefore, it can potentially be developed for the management of burns.


2019 ◽  
Vol 7 (1) ◽  
pp. 19-25
Author(s):  
Ave Olivia Rahman ◽  
Humaryanto Humaryanto

ABSTRACT Background: Some plants, such as betel nuts (Areca catechu), is used as traditional antiseptic. Betel nut mash is applied on ulcus wound.  Fibroblast and collagen are important factor in wound healing.  Research objective : this study is to determine the effect of betel nuts extract on fibroblast and collagen level in full thickness wound healing processs. Methodology: this study used male rats Sprague dawney that randomly divided into 3 groups. Groups I received no treatment, groups II and III received areca catechu extracts with concentration 15% and 30%  respectively. There are 12 rats in each groups. The treatment is given every day without wound’s debridement. Incision were made full thickness with diameter size 1,5 cm, on right back skin was made by lidocain anesthesia subcutaneously. Wound area were measured every days. Termination of rats were done in day 7 and day 16 to histopathology assessment with Haematoxylin-Eosin stain for fibroblast and collagen level by semiqualitative score. Results: level of collagen were higher in group that received extract but level of fibroblast were lower than control group in histopathology of day 7th. Conclusion:  Extract of betel nut increased level of collagen. Keyword : areca catechu, betel nuts, wound, fibroblast, collagen, histopathology   ABSTRAK Latar belakang :  Beberapa tanaman digunakan sebagai antiseptik luka, salah satunya biji pinang  untuk penyembuhan luka ulkus. Fibroblast dan kolagen merupakan salah satu faktor penting dalam penyembuhan luka. Tujuan : Penelitian ini bertujuan mengetahui efek ekstrak biji pinang terhadap level fibroblast dan kolagen pada proses penyembuhan luka full thickness  Metode : Penelitian ini menggunakan tikus galur Sprague dawney jantan berusia 2-3 bulan dan telah mendapatkan persetujuan etik. Setelah aklimatisasi, tikus dibagi secara acak masing-masing 12 ekor dalam 3 kelompok, yaitu kelompok I tanpa perlakuan, kelompok II diberikan salep ekstrak biji pinang 15%, kelompok III diberikan salep ekstrak biji pinang 30%. Luka full thickness dibuat dengan diameter 1,5 cm di daerah punggung belakang bagian kanan dengan anestesi lidokain subkutan. Perlakuan dilakukan setiap hari tanpa debridemen luka. Luas luka diukur  setiap hari. Terminasi dilakukan pada hari ke 7 dan 16 untuk pemeriksaan histopatologi jaringan luka dengan pengecatan Haematoxylin-Eosin. Skoring secara semikualitatif untuk menilai fibroblast dan kolagen. Hasil : Pada kelompok ekstrak pinang terdapat peningkatan level kolagen, akan tetapi tidak terjadi peningkatan level fibroblast pada hasil histopatologi luka kulit hari ke-7.  Kesimpulan :  terjadi peningkatan level kolagen pada pemberian ekstrak biji pinang. Kata kunci : biji pinang, luka, histopatologi, fibroblast, kolagen


Open Medicine ◽  
2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Liu Wei

AbstractBasic experiments have demonstrated that the effect of wound healing in moist environments is better than that in dry environments; therefore, research on moist dressing is the focus of wound healing research. 42 burn patients receiving treatment in Jiangsu Provincial People’s Hospital were selected as experimental cases. Wound surface is divided into treatment group and control group using a self-contrasted method. The treatment group received a moist dressing in the treatment of burn wounds and the control group adopted iodine gauze or Vaseline gauze coverage. Wound healing effect and the impact on the degree of pain of in the two different treatment methods were observed after treatment. The results of 42 patients were included in the analysis. The average healing time of patients’ burn wounds in treatment group is (10.9 3.3) d, and the average healing time in control group is (13.8 3.6) d, so, the difference is significant (P<0.01). Wound pain in the treatment group is significantly lower than that in the control group (P<0.01). Using moist dressing (Mepitel and Mepilex, etc.) in the treatment of burn wounds, woundhealing time can be shortened and wound pain can be reduced significantly.


Biomedicines ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 263 ◽  
Author(s):  
Raedeh Basiri ◽  
Maria T. Spicer ◽  
Cathy W. Levenson ◽  
Michael J. Ormsbee ◽  
Thomas Ledermann ◽  
...  

Trials on nutritional supplements for the treatment of diabetic foot ulcer (DFU) have only evaluated the effects of supplementation with specific nutrients. Additionally, nutrition education has not been a systematic part of these studies. The aim of this study was to evaluate the effects of a nutrient-dense formula combined with nutrition education on wound healing in DFU patients. Twenty-nine patients were randomly assigned to the treatment group (n = 15) receiving two servings of supplements daily plus nutrition education or control group (n = 14) that received the standard of care but no additional nutritional or educational intervention. Both groups were followed for a maximum of 12 weeks. Wound healing, as measured by planimetry, was examined at baseline and every four weeks until complete wound closure or up to 12 weeks. There were no significant differences between groups for BMI, age, duration of diabetes, wound age estimation, or wound area at baseline. The treatment group experienced a faster wound healing rate (6.43 mm2/week more reduction in the wound area) than the control group. The mean reduction in the wound area during the first four weeks of the study was almost 13-fold greater in the treatment group compared to the control group (18.0 mm2/week vs. 1.4 mm2/week, respectively). Our findings showed that nutrition supplementation plus nutrition education significantly accelerated wound healing in DFU patients compared to those who just received a standard-of-care regimen.


Author(s):  
Bimby Irenesia ◽  
Renni Yuniarti ◽  
Endang Mahati

Spirulina platensishas been well-known as an abundant protein and phycocyanin. Spirulina platensis has the effect of anti-inflammatory, so it is widely used to apply cosmetics and biomedicine in the healing process of wounds. To prove that the administration of an extract ofSpirulina platensiscan increase the number of fibroblasts and accelerate the wound healing process in Wistar rats with an incision on their skin. This study used a randomizedpost-test only control group design. The skins of thirty (30) male Wistar rats were incised. This treatment was divided into 4 groups, namely the group that received the 0,1% cream (X1) of S.platensisextract, and the group that was administered with the 0,1% ointment (X2) of S.platensis extract; the negative control group was only treated by a saline solution (C1), and the positive control group was with the administration of Gentamycin 0,1% ointment (C2). The total numbers of fibroblasts were examined on day 14 and calculated using the method of tissue biopsy and hematoxylin-eosin (HE) staining. The wound was checked after 14-day Data analysis was performed with a one-wayANOVA test and was continued by Post Hoc Test LSD. The total numbers of fibroblasts on day 14 from the group of XI, X2, C1, and C2 were 15.00; 17,50; 3,83, 10,33 with p<0.001. The average size of the wounds on day 14 from the groups of X1, X2, C1, and C2 was 551,78; 435,76; 1795,95; 1193,57 with p<0.001, respectively. PostHoc Testresults on the total number of fibroblasts and extensive wound area showed significant differences between them. Administration of the 0.1% ointment of Spirulina platensisextract believed to potentially increasing the number of fibroblasts and accelerate the process of wound healing.


2019 ◽  
Vol 41 (1) ◽  
pp. 131-140
Author(s):  
Jennifer Lynn Schiefer ◽  
Marc Daniels ◽  
Daniel Grigutsch ◽  
Paul Christian Fuchs ◽  
Alexandra Schulz

Abstract Large, superficial burn wounds require many painful dressing changes and, thus, dressings that can stay on the wound and peel off during re-epithelization such as Biobrane® and Suprathel® are preferred, but they are costly. Natural silk has shown good outcomes with respect to wound healing, scarring, and patient satisfaction. This study aimed to evaluate the efficacy of natural silk compared with that of initially used dressings for the treatment of superficial burn wounds greater than 10% of the TBSA. Patients with superficial burns covering &gt;10% of the TBSA were treated with pure silk for the first time (treatment group). Complications during wound healing with respect to the need for further surgery and scarring were compared with those of patients with similar burns of more than 10% TBSA and treated with nylon mesh and collagen instead of silk (treatment group). The treatment and control group comprised 25 and 13 patients, respectively. In total, 88% of patients in the treatment group did not require further treatment, while two patients with chemical burns needed further surgeries. Moreover, patients reported high satisfaction with respect to scarring and aesthetic outcome. Meanwhile, 85% of patients in the control group healed without further surgery and showed higher median hypopigmentation and hyperpigmentation after 12 months. Silk is an effective wound dressing for the treatment of large superficial burn wounds. It avoids painful dressing changes and yields satisfactory aesthetic outcomes. However, especially in large burns, careful initial wound depth assessment is crucial to prevent infection and reoperations.


2018 ◽  
Vol 51 (1) ◽  
pp. 1
Author(s):  
Moh. Basroni Rizal ◽  
Elly Munadziroh ◽  
Indah Listiana Kriswandini

Background: Tooth extraction is the process of removing a tooth from the oral cavity potentially triggering a wound healing response in the body. As a result, many methods have been applied to improve the wound healing process, especially in wounds resulting in complications. One such method involves the application of amniotic membrane which has anti-inflammatory, anti-bacterial, anti-fibrosis, anti-scarring properties with low immunogenicity, epithelialization effects, and secretory leukocyte protease inhibitor (SLPI). It also contains collagen, various growth factors, transferrin, fibronectin, nidogen, proteoglycans, hyaluronan, and laminin. Purpose: This study aimed to determine the effects of sponge amnion on the number of VEGF expressions and new blood vessels in post-tooth extraction wounds of Wistar rats. Methods: Sponge amnion was produced by mixing freeze-dried amnion membrane from the Tissue Bank at RSUD Dr. Soetomo with 1% gelatin before freeze drying the mixture. Wistar rats were then divided into two groups. In Group 1, referred to as the control group, the post-extraction wounds of the rats received no treatment. Meanwhile, in Group 2, the treatment group, the subjects’ post-extraction wounds were treated with sponge amnion. The rats of both groups were sacrificed on day 3 to allow observation of the number of VEGF expressions and new blood vessels. A statistical analysis test, a t-test, was subsequently conducted. Results: There was a significant difference in the number of new blood vessels in the control group and that of the treatment group with a p value of 0.018 (p<0.05). There was also a significant difference in VEGF expression between the two groups with a p value of 0.000 (p <0.05). Conclusion: Sponge amnion can generate a number of VEGF expressions and new blood vessels in the post-extraction wounds of Wistar rats.


Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 878
Author(s):  
Frederick Robert Carrick ◽  
Luis Sebastian Alexis Valerio ◽  
Maxine N. Gonzalez-Vega ◽  
David Engel ◽  
Kiminobu Sugaya

Introduction: Wounds are associated with ranges of simple to complex disruption or damage to anatomical structure and function. They are also associated with enormous economic and social costs, increasing yearly, resulting in a severe impact on the wellbeing of individuals and society. Technology that might accelerate wound healing is associated with many benefits to injured people. Methods: BALBc mice underwent symmetrical excisional wounds through the panniculus carnosus. They were divided into a treatment group placed on an autonomous ceramic far-field infrared blanket (cIFRB) and a control group maintained under standard conditions. We also expanded and cultured adipose tissue-derived mesenchymal stem cells (MSCs) on cIFRB and compared them to standard conditions subjected to a scratch injury to compare survival, proliferation, and wound healing. Results: The wound healing of the cIRFB treatment group was significantly faster than the control group of mice. The wound-healing effect of mesenchymal stem cells on cIRFB was also increased and associated with significant migration to the wound area. Conclusions: Wound healing is improved in a mouse model exposed to cFIRB. The ceramic blanket also promotes survival, proliferation, increased migration, and wound healing of MSCs without affecting their survival and proliferation. The utilization of cFIRB in cellular biology and medical applications may be promising in many situations currently explored in animal and human models. This technology needs no direct or battery power source and is entirely autonomous and noninvasive, making its application possible in any environment.


2021 ◽  
Vol 18 (9) ◽  
pp. 1913-1917
Author(s):  
Xiong Wang ◽  
Li Ma ◽  
Meng-ling Huang ◽  
Yong-gang Chen ◽  
Jin-hu Wu

Purpose: To evaluate the healing efficacy of a Traditional Chinese Medicine (TCM) preparation Zaoxiu Burn Ointment (ZBO) on experimental burns in rats. Methods: The rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and ZBO-treated group. ZBO, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. The observation of wound area contraction and histological analysis of wound tissues were performed. The effect of ZBO on MMPs, VEGF and Type-III collagen proteins of wound tissue in rats were determined by Western blot. Results: After 10 days of topical treatment with ZBO, ZBO-treated group showed faster reduction in wound area in comparison with control groups (p < 0.01). MMP-2, MMP-9, VEGF and type-III collagen expression of the wound tissue increased significantly (p < 0.05) in comparison with the control burn wounds in rats. The ZBO had no cytotoxic effect on BHK-21 cell line. Histological results showed an overall early recovery and regeneration in ZBO-treated group when compared with control group. Conclusion: ZBO possesses significant wound-healing activity in full-thickness burn wounds in rats, and can potentially be developed for the treatment of burns patient in future.


Sign in / Sign up

Export Citation Format

Share Document