scholarly journals Detection and Phylogenetic Analysis of Hepatitis A Virus in the Wastewater Treatment Plant of Ekbatan Town in Tehran, Iran

2022 ◽  
Vol 21 (10) ◽  
Author(s):  
Mitra Nasiri ◽  
Zohreh Yousefi Ghalejoogh ◽  
Angila Ataei-Pirkooh ◽  
Farah Bokharaei-Salim ◽  
Seyed Hamidreza Monavari ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Rna Extraction ◽  
Treatment Plant ◽  
Nested Polymerase Chain Reaction ◽  
Treatment Protocols ◽  
Virus Removal

Background: Limited sources of fresh water necessitate the application of health policies for treatment and decontamination of human sewage for further use. A wide variety of infectious agents, including bacteria, fungi, parasites, and viruses, can be found in sewage. Enteric viruses such as hepatitis A virus (HAV) can survive the current treatments and infect susceptible hosts. Objectives: This study aimed to evaluate the HAV contamination in human sewage before and after treatment in the wastewater treatment plant of Ekbatan town in Tehran, Iran, and analyze the phylogenetic properties of the identified viruses. Methods: Over a 12-month period, we collected the wastewater samples including influent, before chlorination, and effluent, from the wastewater treatment plant of Ekbatan town in Tehran, Iran. Ribonucleic acid (RNA) extraction, complementary deoxyribonucleic acid (cDNA) synthesis, and semi-nested polymerase chain reaction (PCR) were performed to identify HAV contamination. Phylogenetic analysis was performed to investigate subgenotypes of the virus. Results: HAV was detected in all influents and samples before chlorination, while the virus was detected in 50% of the effluent samples. All detected viruses belonged to subgenotype IB. Conclusions: Investigating the presence of HAV in sewage provides a general picture of the virus spread in the population of interest. HAV was detected in all influent samples, indicating that the infection is endemic in this area all year round. This also indicates the inability of the current treatment protocols in virus removal, which can be a threat to the public health.

A mass balance approach to the fate of viruses in a municipal wastewater treatment plant during summer and winter seasons

2013 ◽  
Vol 69 (2) ◽  
pp. 364-370 ◽  
Author(s):  
Katharina Ulbricht ◽  
Hans-Christoph Selinka ◽  
Stefanie Wolter ◽  
Karl-Heinz Rosenwinkel ◽  
Regina Nogueira
Keyword(s):  
Wastewater Treatment ◽  
Mass Balance ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Winter Season ◽  
Treatment Plant ◽  
P Value ◽  
Virus Inactivation ◽  
Virus Removal ◽  
Virus Retention

In contrast to previous discussion on general virus removal efficiency and identifying surrogates for human pathogenic viruses, this study focuses on virus retention within each step of a wastewater treatment plant (WWTP). Additionally, the influence of weather conditions on virus removal was addressed. To account for the virus retention, this study describes a mass balance of somatic coliphages (bacterial viruses) in a municipal WWTP, performed in the winter and summer seasons of 2011. In the winter season, the concentration of coliphages entering the WWTP was about 1 log lower than in summer. The mass balance in winter revealed a virus inactivation of 85.12 ± 13.97%. During the summer season, virus inactivation was significantly higher (95.25 ± 3.69%, p-value <0.05), most likely due to additional virus removal in the secondary clarifier by insolation. Thus, a total removal of coliphages of about 2.78 log units was obtained in summer compared to 1.95 log units in winter. Rainfall events did not statistically correlate with the concentrations of coliphages entering the WWTP in summer.

Collection from Wastewater Treatment Plant, Transportation, and Storage of Raw Wastewater v1

2021 ◽  
Author(s):  
Kevin Calci ◽  
Kevin Calci
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Rna Extraction ◽  
Treatment Plant ◽  
Sequencing Quality ◽  
Project Monitoring ◽  
Pandemic Response ◽  
Wastewater Samples ◽  
And Storage ◽  
Raw Wastewater

This method was developed at the FDA’s Center for Food Safety and Applied Nutrition for GenomeTrakr’s pandemic response project, monitoring SARS-CoV-2 variants in wastewater. Protocols developed for this project cover wastewater collection, concentration, RNA extraction, RT-qPCR detection, library prep, genome sequencing, quality control checks, and data submission to NCBI. This method describes the collection and transport of raw wastewater samples from a wastewater treatment plant.

scholarly journals Prevalence and Genetic Diversity of Aichi Virus 1 from Urban Wastewater in Senegal

Intervirology ◽  
2021 ◽  
pp. 1-6
Author(s):  
Ousmane Kebe ◽  
Maria-Dolores Fernandez-Garcia ◽  
Amary Fall ◽  
Hamet Dia ◽  
Maxime Bidalot ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Indirect Evidence ◽  
Treated Wastewater ◽  
Aichi Virus ◽  
Urban Wastewater ◽  
Wastewater Samples

<i>Aichi virus 1</i> (AiV-1) has been proposed as a causative agent of human gastroenteritis. In this study, raw, decanted, and treated wastewater samples from a wastewater treatment plant in an urban area of Dakar, Senegal, were collected. AiV-1 was detected in raw (70%, 14/20), decanted (68.4%, 13/19), and treated (59.3%, 16/27) samples, revealing a noticeable resistance of AiV-1 to chlorine-based treatment. Phylogenetic analysis revealed that all sequences clustered within genotype B. Our study presents the first report on the detection of AiV-1 in the environment of Dakar and constitutes indirect evidence of virus circulation in the population.

scholarly journals Occurrence and molecular characterization of Torque teno virus (TTV) in a wastewater treatment plant in Tehran

2019 ◽  
Vol 17 (6) ◽  
pp. 971-977
Author(s):  
Shadi Tavakoli Nick ◽  
Seyed Reza Mohebbi ◽  
Seyed Masoud Hosseini ◽  
Hamed Mirjalali ◽  
Masoud Alebouyeh
Keyword(s):  
Wastewater Treatment ◽  
Molecular Characterization ◽  
Wastewater Treatment Plant ◽  
Clinical Symptoms ◽  
Treatment Plant ◽  
Torque Teno Virus ◽  
Treated Wastewater ◽  
Water And Wastewater Treatment ◽  
Nested Polymerase Chain Reaction

Abstract Torque teno virus (TTV) is a single-stranded DNA virus which is predominantly transmitted by the fecal–oral route and may be excreted in the absence of the clinical symptoms. TTV was previously considered a probable cause of hepatitis, but further studies could not strongly connect TTV to any serious health problem. TTV is highly resistant to water and wastewater treatment processes and can be a useful indicator for determining the fecal contamination of water. The purpose of the present study was to assess the prevalence and molecular characterization of TTV in treated wastewater in Tehran. Thirteen effluent samples were collected monthly from the biggest wastewater treatment plant in Tehran, Iran (from September 2017 to August 2018). The presence of the TTV was monitored in the samples by the nested polymerase chain reaction (PCR) method. The TTV genome was found in 76.9% of the samples, and TTV of groups 1 and 3 were determined using phylogenetic analysis. Therefore, treated wastewater can play a key role in the transmission of TTV and the usage of treated wastewater as a source of potable water needs to be carefully controlled.

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