Topical application of TAK1 inhibitor encapsulated by gelatin particle alleviates corneal neovascularization

Neovascularization (NV) of the cornea disrupts vision which leads to blindness. Investigation of antiangiogenic, slow-release and biocompatible approaches for treating corneal NV is of great importance. We designed an eye drop formulation containing gelatin/epigallocatechin-3-gallate (EGCG) nanoparticles (NPs) for targeted therapy in corneal NV. Gelatin-EGCG self-assembled NPs with hyaluronic acid (HA) coating on its surface (named GEH) and hyaluronic acid conjugated with arginine-glycine-aspartic acid (RGD) (GEH-RGD) were synthesized. Human umbilical vein endothelial cells (HUVECs) were used to evaluate the antiangiogenic effect of GEH-RGD NPs in vitro. Moreover, a mouse model of chemical corneal cauterization was employed to evaluate the antiangiogenic effects of GEH-RGD NPs in vivo. GEH-RGD NP treatment significantly reduced endothelial cell tube formation and inhibited metalloproteinase (MMP)-2 and MMP-9 activity in HUVECs in vitro. Topical application of GEH-RGD NPs (once daily for a week) significantly attenuated the formation of pathological vessels in the mouse cornea after chemical cauterization. Reduction in both vascular endothelial growth factor (VEGF) and MMP-9 protein in the GEH-RGD NP-treated cauterized corneas was observed. These results confirm the molecular mechanism of the antiangiogenic effect of GEH-RGD NPs in suppressing pathological corneal NV.

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Treatment of Corneal Neovascularization by Topical Application of Ascorbic Acid in the Rabbit Model

Cornea ◽

10.1097/ico.0b013e318241433b ◽

2012 ◽

Vol 31 (10) ◽

pp. 1165-1169 ◽

Cited By ~ 16

Author(s):

Mee Yon Lee ◽

Sung Kun Chung

Keyword(s):

Ascorbic Acid ◽

Topical Application ◽

Rabbit Model ◽

Corneal Neovascularization

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Inhibition of Pathologic Corneal Neovascularization by Topical Application of a Novel Peptide In Vivo

Cornea ◽

10.1097/ico.0000000000000548 ◽

2015 ◽

Vol 34 (10) ◽

pp. 1295-1302 ◽

Cited By ~ 4

Author(s):

Yi Lu ◽

Yi Xu ◽

Qing Gu ◽

Xun Xu

Keyword(s):

Topical Application ◽

Corneal Neovascularization

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Ultrastructure of Mouse Basal Cell Carcinoma Exhibiting Sebaceous Differentiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600003214 ◽

1980 ◽

Vol 38 ◽

pp. 738-739

Author(s):

Victoria L. Wade ◽

Winslow G. Sheldon ◽

James W. Townsend ◽

William Allaben

Keyword(s):

Basal Cell Carcinoma ◽

Cell Carcinoma ◽

Topical Application ◽

Basal Cell ◽

Sebaceous Gland ◽

Rats And Mice ◽

Mixed Tumors

Sebaceous gland tumors and other tumors exhibiting sebaceous differentiation have been described in humans (1,2,3). Tumors of the sebaceous gland can be induced in rats and mice following topical application of carcinogens (4), but spontaneous mixed tumors of basal cell origin rarely occur in mice.

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