scholarly journals Unusual Etiology of Hypercalcemia in an Adolescent With Acute Gastroenteritis

Cureus ◽  
2021 ◽  
Author(s):  
Madhuradhar Chrgondi ◽  
Ramya Deepthi Billa ◽  
Swathi Chacham ◽  
Shilpa Gurnurkar
Author(s):  
M. F. Miller ◽  
A. R. Rubenstein

Studies of rotavirus particles in humans, monkeys and various non-primates with acute gastroenteritis have involved detection of virus in fecal material by electron microscopy. The EM techniques most commonly employed have been the conventional negative staining (Fig. 1) and immune aggregation (Fig. 2) procedures. Both methods are somewhat insensitive and can most reliably be applied to samples containing large quantities of virus either naturaLly or as a result of concentration by ultracentrifugation. The formation of immune complexes by specific antibody in the immune aggregation procedures confirms the rotavirus diagnosis, but the number of particles per given microscope field is effectively reduced by the aggregation process. In the present communication, we describe use of an on-grid immunoelectron microscopic technique in which rotavirus particles are mounted onto microscope grids that were pre-coated with specific antibody. The technique is a modification of a method originalLy introduced by Derrick (1) for studies of plant viruses.


2018 ◽  
Vol 30 (4) ◽  
Author(s):  
Valentina Daprà ◽  
Ilaria Galliano ◽  
Cristina Calvi ◽  
Paola Montanari ◽  
Massimiliano Bergallo

2020 ◽  
Author(s):  
David Schnadower ◽  
Karen J. O’Connell ◽  
John M. VanBuren ◽  
Cheryl Vance ◽  
Phillip I. Tarr ◽  
...  

Author(s):  
Shuvra Kanti Dey ◽  
Nadim Sharif ◽  
Baki Billah ◽  
Tanjir Tarek Ibn Siddique ◽  
Tarequl Islam ◽  
...  

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