Comparative Assessment of the Nutritional Fitness of Wild and Cultured African Catfish (Clarias gariepinus)

The nutritional qualities of wild and cultured Clarias gariepinus from Oba Reservoir, Ikose, Ogbomoso, Oyo State, Nigeria, and the cultured fish from the Department of Fisheries and Aquaculture, Oyo State Ministry of Agriculture and Mineral Resources, Ogbomoso, Oyo State, Nigeria, were evaluated. The proximate composition, mineral element composition and essential amino acids profile of the sampled fish species were evaluated using standard laboratory procedures. The results of the proximate composition was observed to be high in cultured population compared to the Wild and there was a statistical significant difference in the amount of crude protein, fibres, carbohydrate, fat/lipid, ash and moisture contents across the two sampled populations (Wild; 17.94 ± 0.19, Cultured; 21.06 ± 0.51), t = -5.738, p-value =0.001. Mineral element content showed maximum amount of copper (Cu), Lead (Pb), Manganese (Mn) and Iron (Fe) in Wild population than in their cultured counterpart while the amount of Cadmium (Cd) and Nickel (Ni) observed in Wild population were undetected. It was also discovered that all amino acid analysed were found to be higher in the cultured group compared to the wild. There were varied levels of significant correlations among all the measured amino acids contents across the two sampled populations. This study showed that the proximate composition, elemental contents and amino acids profiles of both the cultured and wild fishes varies significantly and the results proofed that the cultured fish sample were more nutritious than the wild population, therefore, good monitoring system of Oba Reservoir should be encouraged with strict vigilance.

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Proximate composition of male and female African catfish (Clarias gariepinus) and tilapia (Tilapia zilli) in Jega river, Kebbi state, Nigeria

Journal of Fisheries, Livestock and Veterinary Science ◽

10.18801/jflvs.010220.04 ◽

2020 ◽

Vol 01 (02) ◽

pp. 28-35

Author(s):

W. M. Namaga ◽

B. Yahaya ◽

M. A. Salam

Keyword(s):

Protein Content ◽

Fish Species ◽

Lipid Content ◽

Proximate Composition ◽

Clarias Gariepinus ◽

Fibre Content ◽

African Catfish ◽

Male And Female ◽

Juvenile Female ◽

Significant Difference

Fish is highly nutritious, tasty, and easily digestible. It is much sought after by a broad cross-section of the world’s population, particularly in developing countries as it provides the cheapest protein source. Nigeria is blessed with numerous inland freshwater rivers and lakes scattered all over the country. This freshwater habitat consists of many species of fish that have successfully dominated all niches over time and have for many reasons remained unstudied. The situation concealed a lot of scientific information particularly on food security and safety to the inmates and the country at large. Jega River which transcended many States in the North-Western part of Nigeria to open into the river Niger is one of such rivers largely uncared for, study-wise. African catfish (Clarias gariepinus) and Tilapia (Tilapia zillii) have been wisely selected for the present proximate composition studies. The juveniles of these fishes were purchased from local fishermen in Mariner Waje landing site of the river which flows by Jega town, a local government area in Kebbi State, Nigeria. For the study, the methodology approved by the Association of Official Analytical Chemists (AOAC) was used to determine the proximate composition of the fishes. The highest percentage crude protein content of 49.18 ± 0.30% was observed in juvenile female C. gariepinus; while the lowest protein content of 39.22± 0.50% was observed in juvenile male T. zilli. The highest percentage lipid content of 11.75± 1.50% was observed in juvenile female tilapia; while the lowest percentage lipid content of 6.25± 0.29% was observed in male African catfish. The highest percentage fibre content of 4.00 ± 0.29% was observed in male and female C. gariepinus, while the lowest percentage fibre content of 0.75± 0.29% was observed in male tilapia. There were variations (p< 0.05) when the protein and lipid contents of both the species were compared with each other. There was also a significant difference (p< 0.05) when the carbohydrate contents were compared between the fish species, but there was no significant difference (p > 0.05) when the fibre content was compared within the species. The analyses showed that both the male and female fish species studied had high protein contents.

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Comparative Analysis of the Nutritive Value of Smoked, Dried Procambarus clarkii (Freshwater Crayfish) from Three Nigerian States

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2021/v11i330206 ◽

2021 ◽

pp. 39-45

Author(s):

Oluwafemi Akinsola Omole ◽

Oluwafunmike Blessing Adepoju ◽

Oluwasola Toluwalope Moradeyo ◽

Olayinka Josephine Okuneye ◽

Adunola Abosede Bello

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Proximate Composition ◽

Nutritive Value ◽

Procambarus Clarkii ◽

Essential Amino Acids ◽

Freshwater Crayfish ◽

Crude Fibre ◽

Mineral Contents ◽

Significant Difference

The nutritive value of smoked, dried Procambarus clarkii sourced from Akwa Ibom, Rivers and Lagos states of Nigeria were evaluated and compared. The proximate composition of the crayfish samples were determined using official methods of analysis, mineral composition were determined using Atomic Absorption Spectrophotometer and the amino acid composition were analysed using Amino Acid Analyser. The proximate composition of the crayfish samples from three different locations (Akwa Ibom, Lagos and Rivers states) such as moisture, crude protein, crude fat and ash contents showed significant difference (p<0.05) across the selected locations while crude fibre and carbohydrate content showed no significant difference (p<0.05) across the selected locations. The amino acids composition gave the crayfish sample from Akwa Ibom state as the richest source of essential amino acids. The mineral contents of the crayfish samples such as potassium, phosphorus, magnesium, manganese and calcium showed significant difference (p<0.05) across the selected locations while calcium, zinc and sodium contents showed no significant difference (p<0.05) across the selected locations. Comparing their nutrient component, smoked, dried P. Clarkia from Awka Ibom state possess the richest nutritive value.

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Comparative Study of the Flesh Quality of Clarias gariepinus in Farm-raised and Wild Populations

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2019/v4i430060 ◽

2019 ◽

pp. 1-9

Author(s):

Popoola Omoniyi Michael ◽

Fasakin Emmanuel Adedayo

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Clarias Gariepinus ◽

African Catfish ◽

Mean Values ◽

Significant Difference ◽

Amino Acids Profile

Aim. Nutrients analysis of wild and farmed raised African catfish (Clarias gariepinus, Burchell, 1822) were studied. The comparative work was carried out to find out if habitat could affect the nutrient composition of the fish. Study design: Five live C. gariepinus were obtained for three wild location and three fish farm each (n=30). One-way analysis of variance was use to examine the nutrients composition across the study locations. Methodology: Five individual fishes were randomly selected from the forty individuals in a population obtained from six locations. They were sacrificed and cut into three (tail, middle and trunk) chunks. These were oven dried at a constant temperature of 105ºC using oven model LCON53CF. The samples were later blend using electric blender and kept in airtight nylon for further analysis. Standard methods were employed in the analysis of body nutrients. Results: A significant difference was observed in proximate composition of C. gariepinus on dry matter bases. The moisture and lipids content were lower in wild C. gariepinus (5.16±0.07;15.27+0.08%) than farm raised (5.25±0.10%;18.54+0.08%). The average protein and ash contents in farm-raised C. gariepinus were significantly (p<0.05) lower (66.23+0.08%; 5.00+0.07%) than wild C. gariepinus (67.24+0.09%;9.06+0.07%). Organoleptic study revealed differences in taste between wild and farm raised C. gariepinus (p<0.05). The amino acids profile showed little disparity in quantity in wild and farm raised C. gariepinus. No significant difference (p>0.05) was noticed in the mean values of amino acids in wild (4.21) and farmed raised (4.16) C. gariepinus. The percentage of saturated fatty acids was higher (56.24%) in farmed raised C. gariepinus than the wild stocks (44.53%) unlike the unsaturated fatty acids that was higher in wild (79.46%) than the farmed raised (54.03%). Conclusion: The study revealed that wild fish possess some nutritional advantages over the culture C. gariepinus.

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Impact of Ogun River pollution on antioxidants and proximate composition of African catfish (Clarias gariepinus)

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i3.955 ◽

2019 ◽

Vol 46 (3) ◽

pp. 163-184

Author(s):

B. O. Onunkwor ◽

T. O. Akinpelu ◽

O. J. Oladipupo ◽

R. N. Ugbaja ◽

O. J. Olaoye ◽

...

Keyword(s):

Proximate Composition ◽

Clarias Gariepinus ◽

Chemical Properties ◽

Oxygen Demand ◽

Control Fish ◽

Average Weight ◽

African Catfish ◽

Fish Samples ◽

The Impact ◽

Ogun River

Most rivers in Nigeria are polluted without conscious efforts to abate ensuant consequences. Thus, Clarias gariepinus, an aquatic commercial food from Ogun River, Nigeria, was studied to investigate the impact of pollution on antioxidants and proximate composition. During the rainy season, 50 C. gariepinus (average weight 426.53 ± 24.40g and length 37.73 ± 0.95cm) were site sampled, 10 from each of Ago-Odo, Ago-Ika, Oke-Sokori, and Quarry along Ogun River course. Ajegunle-Adao cultured pond, served as control. The parameters assayed for included: some heavy metals and physico-chemical properties of sampled water, antioxidants and proximate composition of the fish samples. Physicochemical parameters of river studied showed that temperature of all sites ranged from 26 to 29°C, pH: 6.9 to 7.8, dissolved oxygen: 9.5 to 21.6, biochemical oxygen demand: 3.60 to 14.10, and chemical oxygen demand: 60 to 650. Lead (Pb) concentration ranged from 4.0 to 17.0 μg/L and cadmium (Cd): 0.0 to 6.0 μg/L. Blood Pb, Cd, activities of: superoxide dismutase, catalase, glutathione-S-transferase, plasma xanthine oxidase, and lipid peroxidation level of fish samples from Ago-Ika were most significantly (p < 0.05) high, with significant (p < 0.05) lowest reduced glutathione concentration amongst the samples from polluted sites when compared to control fish. Proximate analysis showed that the fish samples from Ogun River had significantly (p < 0.05) lower protein, fat contents, and higher nitrogen-free extract while Ago-Ika, Oke-Sokori and Quarry sites had significantly (p < 0.05) lower moisture, ash and fibre contents when compared with control. It can be concluded that the impact of pollution induced oxidative stress and significantly diminished the nutritional value of C. gariepinus from Ogun River.

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Nutritional Analysis and Quality Evaluation in Muscle of Crucifix Crab Charybdis feriatus From Three Wild Populations

Journal of Food Research ◽

10.5539/jfr.v3n2p27 ◽

2014 ◽

Vol 3 (2) ◽

pp. 27 ◽

Cited By ~ 2

Author(s):

Jian-xue Lu ◽

Yang-yang Gong ◽

Yan-qing Huang ◽

Hong-yu Ma ◽

Xiong Zou ◽

...

Keyword(s):

Amino Acids ◽

Fatty Acid ◽

Unsaturated Fatty Acids ◽

Fatty Acid Content ◽

Total Content ◽

Essential Amino Acids ◽

Maturation Stage ◽

Wild Populations ◽

Nutritional Analysis ◽

Significant Difference

Three wild populations of crucifix crab Charybdis feriatus were sampled and muscle was analyzed for proximate composition, amino acid and fatty acid composition, aimed to quantify and compare the nutritional quality from three different locations in China. Results showed that crude protein content in muscle of female crucifix crab C. feriatus from Zhoushan (ZS) and Xiapu (XP) (84.84%-88.35%) were significantly higher than that of crucifix crab C. feriatus from Qionghai (QH) (74.33%), while there was no significant difference in terms of crude fat content (3.82%-4.07%). The highest content of ash was found in muscle of crucifix crab C. feriatus from QH (5.36%). The muscle of crucifix crab C. feriatus from ZS and XP had significantly higher contents of total amino acids, essential amino acids, non-essential amino acids and delicious amino acids than those of QH group (P < 0.05). The total saturated and unsaturated fatty acid content in the muscle of crucifix crab C. feriatus from three wild populations showed significant difference (P < 0.05). The ZS group had highest content of poly-unsaturated fatty acids (33.64%) and total content of EPA and DHA (22.85%) as well, followed by XP and QH group (P < 0.05). Overall, the differences in chemical composition in muscle of C. feriatus from different locations could be attributed to environmental variables and maturation stage.

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Reproductive Performances and Egg Qualities in African Catfish (Clarias gariepinus) Broodstocks Supplemented with Curcumin and Thyroxine Hormone

Omni-Akuatika ◽

10.20884/1.oa.2020.16.1.696 ◽

2020 ◽

Vol 16 (1) ◽

pp. 32

Author(s):

Livana Dethris Rawung ◽

Damiana Rita Ekastuti ◽

Muhammad Zairin Junior ◽

Min Rahminiwati ◽

Ade Sunarma ◽

...

Keyword(s):

Reproductive Performance ◽

Egg Quality ◽

Gonadosomatic Index ◽

Clarias Gariepinus ◽

Density Lipoprotein ◽

Cholesterol Concentration ◽

Hepatosomatic Index ◽

African Catfish ◽

Hatching Rate ◽

Significant Difference

An experiment was designed to study the effects of curcumin and thyroxine hormone supplementation in the diet to improve the reproductive performance of catfish broodstock by improving egg quality that eventually increases the production of seedlings. Catfish used in this experiment were supplemented with curcumin and thyroxine hormone through their feeds for 12 weeks. The results showed that there was no significant difference (p > 0.05) in the hepatosomatic index (HSI), gonadosomatic index (GSI), percentage of gonad maturity, total cholesterol concentration in the spawned eggs, high density lipoprotein (HDL) concentration in the spawned eggs, fertilization rate of spawned eggs, and hatching rate of fertilized eggs. However, there were significant differences (p < 0.05) in the concentration of vitellogenin in the spawned eggs, egg diameter of the spawned eggs, and the triglycerides contents of spawned eggs. It was concluded that curcumin and thyroxine supplementations of African catfish increased vitellogenin concentrations and diameters of spawned eggs that have great potential to improve the reproductive performance.

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Assessment of Cinnamon (Cinnamomum verum) Bark Extract on Proximate Composition and Sensory Qualities of Smoked-Dried African Catfish Clarias gariepinus (Burchell, 1822)

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2021/v14i130285 ◽

2021 ◽

pp. 1-6

Author(s):

M. Y. Haruna ◽

M. M. Bello ◽

M. A. Dadile ◽

A. M. Mohammed

Keyword(s):

Sensory Evaluation ◽

Proximate Composition ◽

Clarias Gariepinus ◽

Economic Loss ◽

Mean Value ◽

Carbohydrate Content ◽

Bark Extract ◽

African Catfish ◽

Cinnamon Bark ◽

Extract Solution

The study determined the effect of Cinnamon (Cinnamonum verum) bark extract solution on proximate composition and sensory evaluation of smoke-dried catfish stored at ambient temperature (37+2oC) for 28 days. The experimental treatments are the control, 0.5%, 1% and 1.5 %( w/v) cinnamon bark extract solutions. Thirty-nine fish of average mean weight of 24.87+1.25g were gutted, washed and randomly assigned to the treatments. Thereafter, the fish were soaked into the treatments for 30minutes and later hot smoked for 12hours. After smoking, the fish were stored in boxes and placed on laboratory table for one month. The catfish Clarias gariepinus treated with 1% solution of Cinnamon bark extract had the highest moisture content with mean value 13.65+0.82%, followed by 6.93+1.66% recorded in fish sample treated with 1.5% solution of the extract, lowest percentage crude protein content 38.15+0.82% was observed in fish treated with 1% concentrate of the extract and percentage carbohydrate content indicate that the control have the lowest carbohydrate content with mean value 1.92+0.01% which is significantly different (p<0.05) from other treated sample while sample treated with 1.5% solution of the Cinnamon bark extract have the highest percentage carbohydrate with mean value 15.73+1.64%.Sensory evaluation was also assessed using 7-point hedonic scale and reveals that the smoked catfish retained very good score for appearance, colour, flavour, texture and general acceptance after 28days of storage and the overall acceptability mean score in fish treated with 5g and 15g cinnamon bark extract solution indicate the product is being generally accepted (2.87+0.59%) and (2.34+0.56%) respectively. Cinnamon (Cinnamonum verum) bark extract solution could be used to protect stored smoke-dried catfish from loss of nutritional value and general acceptability thus limiting economic loss and possible health risk to consumers.

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Comparative study on African catfish (Burchell, 1822) hypophysation using ovaprim and chicken pituitary gland extract

Global Journal of Fisheries Science ◽

10.31248/gjfs2021.021 ◽

2021 ◽

Vol 3 (1) ◽

pp. 1-6

Author(s):

O. A. Babalola ◽

◽

F. A. Fakunmoju ◽

T. F. Sunnuvu ◽

B. E. Faleti ◽

...

Keyword(s):

Water Quality ◽

Comparative Study ◽

Pituitary Gland ◽

Clarias Gariepinus ◽

Latency Period ◽

Quality Parameters ◽

Water Quality Parameters ◽

African Catfish ◽

Gland Extract ◽

Significant Difference

Comparative study on African catfish hypophysation indices using ovaprim and chicken pituitary gland extract (CPGE) was carried out. Sixteen (16) African catfish brood stocks between 1100 and 2300 g in ratio 2:1 and four (4) numbers of layer birds (Gallus gallus domesticus) between 1200 and 2200 g in ratio 1:3 were used respectively. The experiment was batched into “A” and “B”. Batch ‘A’ was placed on ovaprim while batch ‘B’ was placed on CPGE. An indoor hatchery vat, measuring 0.6096 m x 1.8288 m x 0.3048 m each was used for the incubation. Digital measuring kits were used to monitor water quality parameters and measurement of the brood stocks weights independently. Brood stocks on ovaprim and CPGE treatments had the following mean hypophysation indices in these order: fecundity (11100±7690 and 17760±13863), latency period (9.53±0.54 and 9.77±0.49), fertilization percentage (94.03±5.90% and 90.6±10.11%), hatchability percentage (75.6±0.81% and79.35±10.27%), number of eggs produced (5889±657.37 and 9403.33±589.80), total number of larvae hatched (4717.33±1111.0 and 7111.33±506.64), SGR (0.67±0.31 and 1.1±0.31), and survival rate (64.81±0.71 and66.24±2.19) respectively. There was no significant difference (p = 0.05) in the mean values of fecundity, latency period, fertilization and hatchability percentages in both treatments but there was significant difference (p ≤ 0.05) in number of eggs produced and total number of larvae hatched in both treatments. The water quality parameters were within the range for induced breeding techniques and there was no significant difference (p= 0.05) in both experiments. Both ovaprim and CPGE had excellent results from the induced breeding of Clarias gariepinus and yielded good results. Therefore, CPGE could be recommended from the view point of excellent results and its availability from chicken slaughter houses as against ovaprim which are imported.

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Effect of environmental regulated water temperature variations on survival, growth performance and haematology of African catfish, Clarias gariepinus

Our Nature ◽

10.3126/on.v15i1-2.18791 ◽

2017 ◽

Vol 15 (1-2) ◽

pp. 26-33

Author(s):

Johnny O. Ogunji ◽

Jude Awoke

Keyword(s):

Blood Cell ◽

Water Temperature ◽

Growth Performance ◽

Crude Protein ◽

Clarias Gariepinus ◽

African Catfish ◽

Good Growth ◽

Corpuscular Haemoglobin ◽

Significant Difference ◽

Mean Corpuscular Haemoglobin

A 56 day study was carried out to evaluate effect of temperature changes on survival, growth performance and haematology of Clarias gariepinus fingerlings fed housefly maggot meal (magmeal) diet. Ninety (90) Clarias gariepinus fingerlings, (initial mean weight 4.33±0.03g) were subjected to different environmental regulated temperature conditions in three locations (laboratory, outdoor, greenhouse). The observed average temperature was 26.53±0.01°C, 26.06±0.01°C and 31.52±0.00°C for laboratory, outdoor, greenhouse, respectively. Ten fingerlings stocked per experimental tank were fed in triplicates at 5% body weight in two portions per day. It was observed that different water temperatures affected fish growth. All experimental fish in the greenhouse died after 8 days of exposure. This happened around 14.00 hrs when water temperature reached 40°C. There was no significant difference (P<0.5) in final weight, weight gain, food conversion ratio and standard growth rate among the fish reared in the laboratory (26.53°C) and outside tanks (26.06°C), respectively. The initial carcass crude protein (Cp) value was 58.97%. At the end of the experiment fish reared in the laboratory had a crude protein value of 63.97±0.06% Cp and those reared outdoor (26.06°C) had 71.28±0.00% Cp. No significant difference in values of packed cell volume; white blood cell; haemoglobin and mean corpuscular haemoglobin concentration was found between fish reared in laboratory (26.53°C) and outside (26.06°C). However, the red blood cell (RBC); mean corpuscular haemoglobin and mean corpuscular volume showed significant difference (P>0.05). The result confirms that Clarias gariepinus fingerlings reared at a mean temperature of 26°C (within the recorded optimal temperature range for good growth) performed well. Clarias gariepinus fingerlings are not able to survive when water temperature reaches to 40oC.

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Pengaruh pemberian mikrokapsul probiotik Bacillus cereus P22 dan Staphylococcus lentus L1k pada pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele, Clarias gariepinus Burchell 1822 yang diinfeksi Aeromonas hydrophila

Jurnal Iktiologi Indonesia ◽

10.32491/jii.v17i2.354 ◽

2018 ◽

Vol 17 (2) ◽

pp. 143

Author(s):

Lilik Setiyaningsih ◽

Widanarni Widanarni ◽

Angela Mariana Lusiastuti3 ◽

Munti Yuhana

Keyword(s):

Bacillus Cereus ◽

Growth Performance ◽

Aeromonas Hydrophila ◽

Clarias Gariepinus ◽

Bacterial Count ◽

Negative Control ◽

Total Bacterial Count ◽

African Catfish ◽

Feed Conversion ◽

Significant Difference

The aimed of this research was to evaluate the effects of dietary probiotic microcapsules B.cereus P22 and S. lentus (L1k) at different dose and frequency on growth performance, immune response and resistance of African catfish infected with A. Hydrophila. Probiotics used in this study were B. cereus P22 and S. lentus L1k encapsulated by spray draying method. The research was carried out for 56 days with eight treatments and four replications. The treatments were K- (negative control), K+ (positive control), A (feed supplemented with 0,5% of microencapsulated probiotic, fedevery day), B (feed supplemented with 0,5% of microencapsulated probiotic, fed once every three days), C (feed supplemented with 1% of microencapsulated probiotic, fed every day), D (feed supplemented with 1% of microencap-sulated probiotic with an administration once every three days), E (feed with 2% of microencapsulated probiotic with an administration every day) and F (feed with 2% of microencapsulated probiotic with an administration once every three days). On day 42, all of the fish except K- were challenged by intramuscular injection of A. hydrophila (108 CFU ml-1). In 40 days after infection, there were no significant difference on survival rate (SR) between treatments (p> 0.05). Treatment E displayed the higher growth rate (4,54±0,02%), total probiotic B. cereus (P22) and S . lentus (L1k) (4.06± 0.09 log CFU g-1; 4.02±0.08 log CFU g-1) than other treatments; whereas treatment D showed the best feed conversion rasio(1.191±0.013), and treatment F offered the highest total bacterial count (7.11±0.53 log CFU g-1). An administration of 2% microencapsulated probiotic in every day frequency demonstrated the better growth performance, immune res-ponse and resistance of African catfish to A. hydrophila compare with other treatments. AbstrakPenelitian ini bertujuan untuk mengkaji dosis dan frekuensi pemberian mikrokapsul probiotik melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele yang diinfeksi Aeromonas hydrophila. Penelitian ini meng-gunakan Bacillus cereus P22 dan Staphylococcus lentus L1k yang telah dienkapsulasi melalui metode spray draying. Penelitian ini dilaksankan selama 56 hari dengan delapan perlakuan dan empat ulangan, terdiri atas perlakuan K- (kon-trol negatif), K+ (kontrol positif), A (pakan+mikrokapsul probiotik dosis 0,5% frekuensi setiap hari), B (pakan+mikro-kapsul probiotik dosis 0,5% frekuensi tiga hari sekali), C (pakan+mikrokapsul probiotik dosis 1% frekuensi setiap hari), D (pakan+mikrokapsul probiotik dosis 1% frekuensi tiga hari sekali), E (pakan+mikrokapsul probiotik dosis 2% fre-kuensi setiap hari) dan F (pakan+mikrokapsul probiotik dosis 2% frekuensi tiga hari sekali). Ikan diuji tantang dengan A. hydrophila pada hari ke 42 dengan kepadatan 108 CFU ml-1 secara intramuscular (kecuali K- diinjeksi dengan phos-phate buffer saline). Setelah 40 hari pascainjeksi, laju sintasan tidak menunjukkan perbedaan yang signifikan antarperlakuan (p>0,05).Perlakuan E menunjukkan laju pertumbuhan (4,54±0,02%) dan total probiotik B. cereus (P22) dan S. lentus (L1k) (4,06±0,09 log CFU g-1; 4,02±0,08 log CFU g-1) tertinggi; sementara perlakuan D memberikan hasil nisbah konversi pakan terbaik (1,191±0,013), perlakuan F menunjukkan total bacterial count tertinggi (7,11±0,53 log CFU g-1). Hasil penelitian menunjukkan bahwa dosis 2% yang diberikan setiap hari memberikan hasil yang lebih baik dalam meningkatkan laju pertumbuhan, respons imun, dan resistensi ikan lele terhadap A. hydrophila.

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