scholarly journals Immunomodulatory Activity on Specific Immune Function in Albino Mice Exposed to Extract and Fractions of Zapoteca portoricensis (Jacq) HM. Hernández Roots

2021 ◽  
pp. 41-50
Author(s):  
Oghenesuvwe D. Warren ◽  
Bonaventure C. Obi ◽  
Uchenna C. Abonyi ◽  
Theophine C. Akunne
Keyword(s):  
Ethyl Acetate ◽  
Normal Control ◽  
Ethyl Acetate Fraction ◽  
Immunomodulatory Activity ◽  
Neutrophil Adhesion ◽  
Root Extract ◽  
Percentage Increase ◽  
Methanol Fraction ◽  
Group I ◽  
Study Results

Zapoteca portoricensis (Jacq) HM. Hernández popularly called “Elugelu” belongs to the Fabaceae family. It is reportedly used in traditional medicine as anti-diarrhoea, anticonvulsant, antispasmodic and in management of autoimmune disorders. The immunomodulatory activity of methanol root extract and fractions of Zapoteca portoricensis was evaluated using three experimental models: neutrophil adhesion, haemagglutination antibody (HA) titre and delayed hypersensitivity (DTH) of an immune response in mice. Five (5) groups of mice (n=5) were used; group I was the normal control; group II was the standard control (levamisole, 2.5 mg/kg) while groups IIIA–C, IVA–C and VA–C received graded doses (25, 50, 100 mg/kg) of methanol extract (ME), ethyl acetate fraction (EF) and methanol fraction (MF), respectively. Sheep red blood cells (SRBCs; 0.1 ml) were injected subcutaneously to sensitize the animals. The study results showed that the methanol fraction (MF) exhibited the highest percentage (46.12%) in neutrophil adhesion followed by the ethyl acetate fraction (EF) (37.06%) at 100 mg/kg dose, respectively, compared with the normal control. The highest percentage increase in both primary and secondary antibody titre was found to be ME (85.19%, 43.20%), EF (91.53%, 102.67%) and MF (128.31%, 111.89%) at 50 mg/kg dose, respectively compared to the normal control. The EF at the doses of 25 and 50 mg/kg produced the highest percentage inhibition (56.57%, 58.33%) in DTH response, respectively, followed by MF (42.46%) at a dose of 25 mg/kg. Oral administration of Z. portoricensis exhibited immunomodulatory effects on specific components of the immune system in mice.

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 525
Author(s):  
Ayu Natasya Paputungan ◽  
Widya Astuty Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Methanol Fraction ◽  
Tlc Bioautography ◽  
Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against  Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography.  ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode  fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol  dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap  Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

scholarly journals SYZYGIUM CUMINI (L.) SKEELS LEAF EXTRACT FRACTIONS AS ARGINASE INHIBITORS AND THE EFFECTS OF TANNINS ON THEIR ACTIVITY

2020 ◽  
pp. 268-270
Author(s):  
ARI ARIEFAH HIDAYATI ◽  
BERNA ELYA ◽  
RANI SAURIASARI
Keyword(s):  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Colorimetric Method ◽  
Ethyl Acetate Fraction ◽  
Precipitation Method ◽  
Total Phenolic ◽  
Syzygium Cumini ◽  
Methanol Fraction ◽  
Phenolic And Flavonoid

Objective: Arginase inhibition could be a potential therapeutic approach for endothelial dysfunction. Syzygium cumini (L.) Skeels leaves containphenolic acids and flavonoids, which have been predicted to exhibit arginase inhibitory activity. Moreover, these leaves contain tannins, which canform complexes with enzymes and lead to false-positive results during biological testing. Therefore, this study was conducted to evaluate the arginaseinhibitory activity of S. cumini leaf extract and fractions as well as to elucidate the effects of tannins on this activity.Methods: S. cumini leaves were fractionated using n-hexane, ethyl acetate, and methanol. A colorimetric method was employed to evaluate arginaseinhibitory activity. Tannin elimination was performed through the gelatin precipitation method. Total phenolic and flavonoid contents of the fractionswere calculated using the Folin–Ciocalteu and aluminum chloride methods, respectively.Results: Ethyl acetate and methanol fractions showed arginase inhibitory activity with half-maximal inhibitory concentrations (IC50) of 46.96 and15.35 μg/mL, respectively. The methanol fraction was positive for tannins. After tannin elimination, this fraction exhibited less potent arginaseinhibitory activity, with an IC50 value of 53.03 μg/mL. The ethyl acetate fraction showed higher total phenolic and flavonoid contents than the methanolfraction.Conclusion: Tannins affected the arginase inhibitory activity of the methanol fraction of S. cumini leaves; however, the ethyl acetate fraction did notcontain tannins and could inhibit arginase activity.

scholarly journals Effect of Root Extract Fractions of Kyllinga triceps Rottb on Streptozotocin Induced Diabetic Rats

1970 ◽  
Vol 4 (1) ◽  
pp. 25-30
Author(s):  
Swaroopa Rani Vanapatla ◽  
G Krishna Mohan ◽  
B Ravi Kumar
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Ethyl Acetate ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rats ◽  
Root Extract ◽  
Neonatal Rats ◽  
Acute Study

The present study was aimed to evaluate the root extract fractions of Kyllinga triceps (KT) for their antidiabetic potential on streptozotocin induced diabetes in neonatal rats. Diabetes was induced by a single intraperitoneal injection of Streptozotocin (90mg/kg) to 48±2h old neonatal rats. Effect of root extract fractions (toluene, ethyl acetate, 1- butanol at 50 &100 mg/kg.) were tested for their antihyperglycemic activity by measuring their fasting blood glucose level in diabetic rats at 0,2,4,6,8,12 & 24 h after the treatment. In sub acute study ethyl acetate fraction of KT (EAKT) was administered daily to diabetic rats orally at a dose of 100mg/kg for 28 days. Body weight of the animals and blood glucose level were observed at weekly interval during the study. Cholesterol, triglycerides, insulin, SGPT, ALP, creatinine and total proteins level in serum were also estimated at the initial and after 28 days of the treatment. As the preliminary investigation conducted in our lab on methanolic extract of the roots of KT had showed significant oral glucose tolerance with 200 mg/kg in normal rats. Oral administration of fractions of the plant significantly reduced the fasting blood glucose level in diabetic rats. Among the fractions, EAKT was found to be more effective. Further, in sub-acute study, EAKT, showed a significant anti diabetic activity by reversal of the altered afore said serum biochemical parameters. The results of the study are substantiating the traditional claim of the roots of Kyllinga triceps in the treatment of diabetes with a scope for development of antidiabetic herbal drug from EAKT.   Key words: Antidiabetic activity; Kyllinga triceps; Ethyl acetate fraction; Streptozotocin. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8863 SJPS 2011; 4(1): 25-30

scholarly journals Phytochemical, antimicrobial, antioxidant and enzyme inhibitory potential of medicinal plant Dryopteris ramosa (Hope) C. Chr.

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fiaz Alam ◽  
Syed Hurmat Ali Khan ◽  
Mohammad Hassham Hassan Bin Asad
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Methanol Fraction ◽  
Medicinal Uses ◽  
Antibiotic Effect ◽  
Esterase Inhibition ◽  
Mic Value

Abstract Background Dryopteris ramosa has numerous potentials uses in the treatment of different maladies as old traditional medication. The fronds of D. ramose are edible and orally administered for producing antibiotic effect. They are also used as astringent and febrifuge, and as a pesticide. Methods Extraction of fronds of D. ramosa using solvents of increasing polarity, namely, ethyl acetate, methanol and water were tested for phytochemical (qualitative tests, GC-MS), antimicrobial (well method), antioxidant (DPPH), antifungal (tube dilution), cytotoxic activity (brine shrimps lethality assay) and LOX and COX inhibitory activities were performed using standard methods. Results The phytochemical analysis of the crude methanolic extract revealed that the fronds are rich in flavonoids, alkaloids, saponins, tannins, glycosides and triterpenoids. The total flavonoid content of the ethyl acetate fraction was 46.28 μg QE/mg extract. The GC-MS analysis revealed nine major compounds that constituted the crude drug and potentially had a role in reported activities. The crude extract was the most active amongst all the fractions against the bacterial and fungal strains used such that it inhibited the growth of P. aeruginosa with a zone of 13 mm and a MIC value of 16 μg/ml as compared to the standard cefixime, which inhibited the zone by only 10 mm and a MIC value of 32 μg/ml. The highest antioxidant potential in DPPH assay was shown by the crude extract with 91.948% free radical scavenging activity. The bring shrimps lethality potential of the crude extract was the highest, with a LD50 value of 47.635 μg/ml. The ethyl acetate fraction inhibits 91.36% of alpha glucosidase enzyme at a concentration of 0.5 mg/ml. In case of acetylcholine esterase inhibition assay, the methanol fraction inhibits 58.26% of the enzyme activity. Similarly, for butyrylcholine esterase inhibition, the maximum inhibitory effect was seen in the methanol fraction, with a percentage inhibition of 47.32%. Conclusion These test results support traditional medicinal uses of the plant. Dryopteris ramosa could be imperative for being used as a therapeutic agent and the medicinal importance of this plant should be further investigated.

scholarly journals Immunomodulatory Activity of Phyllanthus maderaspatensis in LPS-Stimulated Mouse Macrophage RAW 264.7 Cells

Separations ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 129
Author(s):  
Uoorakkottil Ilyas ◽  
Deepshikha P. Katare ◽  
Punnoth Poonkuzhi Naseef ◽  
Mohamed Saheer Kuruniyan ◽  
Muhammed Elayadeth-Meethal ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Raw 264.7 Cells ◽  
Immunomodulatory Activity ◽  
No Production ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Significant Stimulatory Effect

Phyllanthus species (Family Euphorbiaceae) has been used in traditional medicine of several countries as a cure for numerous diseases, including jaundice and hepatitis. This study is an attempt to evaluate the immunomodulatory activity of various fractions, column eluents of ethyl acetate fraction, and their polyphenols. Phyllanthus maderaspatensis were standardized using high-performance liquid chromatography to identify and quantify polyphenols, and purification of polyphenols was carried out using vacuum liquid chromatography. Subsequently, we tested various fractions, column eluents of ethyl acetate fraction, and polyphenols in vitro to assess their impact on nitric oxide (NO) production in LPS-stimulated mouse macrophage RAW 264.7 cells. The ethyl acetate fraction (100 μg mL−1) had a more significant stimulatory effect on LPS-stimulated NO production by the RAW 264.7 cells. We found that the ethyl acetate fraction contains a high amount of catechin, quercetin, ellagic acid kaempferol, and rutin, which are responsible for immunomodulation. The ethyl acetate fraction at concentrations of 25 and 50 μg mL−1 had a significant inhibitory effect and 100 μg mL−1 had a more significant stimulatory effect when compared with the LPS control. The percentage of inhibition by LPS control ranged from zero percentage, kaempferol ranged from 45.4% at 50 μg mL−1 to 41.88% at 100 μg mL−1, catechin ranged from 50% at 50 μg mL−1 to 35.28% at 100 μg mL−1, rutin ranged from 36.2% at 50 μg mL−1 to 47.44% at 100 μg mL−1, gallic acid ranged from 28.4% at 50 μg mL−1 to 50.9% at 100 μg mL−1, ellagic acid ranged from 45.12% at 50 μg mL−1 to 38.64% at 100 μg mL−1, and purified quercetin ranged from 26.2% at 50 μg mL−1to 45.48% at 100 μg mL−1. As NO plays an important role in the immune function, polyphenols’ treatment could modulate several aspects of host defense mechanisms owing to the stimulation of the inducible nitric oxide synthase.

scholarly journals Skrining Fitokimia dan Uji Aktivitas Antibakteri Fraksi Etil Asetat dan n-Heksana dari Daun Mangga Kasturi (Mangifera casturi Kosterm.)

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 227-233
Author(s):  
Dwi Lestari ◽  
Desy Fitriani ◽  
Serli Anngraeni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Study Results ◽  
Mango Leaves ◽  
Weak Antibacterial Activity

Kasturi mango (Mangifera casturi Kosterm.) is a typical mango of South Kalimantan. Kasturi mango leaves are reported to have antioxidant activity and are potential for treating various diseases, including diseases related to antibacterial. This study examines the antibacterial activity of ethyl acetate fraction and the n-hexane fraction of mango musk leaves on bacteria that cause acne. Experimental research started with plant determination, making extracts and fractions, phytochemical screening, and antibacterial testing against Staphylococcus aureus and Propionibacterium acnes using the disk diffusion method. The study results found that the ethyl acetate and n-hexane fractions had weak antibacterial activity against S. aureus and P. acnes bacteria, which cause acne

ETHYL ACETATE EXTRACT OF Chenopodium murale ROOT, A SOURCE OF BIOACTIVE COMPOUNDS

2021 ◽  
Vol 27 (1) ◽  
pp. 93-100
Author(s):  
Arshad Javaid ◽  
Syeda Fakehha Naqvi ◽  
Iqra Haider Khan
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Methyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Ethyl Acetate Fraction ◽  
Dioctyl Phthalate ◽  
Literature Survey ◽  
Root Extract ◽  
Chenopodium Murale

Chenopodium murale L. is a winter weed of Chenopodiaceae. In this study, bioactive compounds present in ethyl acetate fraction of root extract of C. murale were identified. The weed plants were collected from Jehlem, Pakistan. Its roots were dried, powdered and extracted in methanol. After evaporation of the solvent, the remaining extract was mixed in water and partitioned with n-hexane, chloroform and finally with ethyl acetate. The last fraction was analyzed through GC-MS that indicated the presence of 15 compounds. These included the three major compounds namely o-xylene (15.03%), cyclopentanol (13.42) and 2-hexanol (13.99%). The moderately and less abundant compounds were ethylbenzene (5.47); methyl acetate (6.00%); cholestrol (4.33%); 2-phenanthrenol (3.01%); cyclohexanone (5.32%); p-xylene (5.12%); furostan-3,26-diyl dibenzoate (3.29%); dihexyl phthalate (4.99%); tricosanoic acid (2.74%); dioctyl phthalate (4.99%), hexanal (3.05%) and ergostane (1.29%). Literature survey showed that 10 of the identified compounds exhibited various biological activities including antifungal, antibacterial, antioxidant, anticancer and antipsoriatic. Most of the compounds were antimicrobial in nature.

scholarly journals Evaluation of Antibacterial, Antineoplastic, and Immunomodulatory Activity ofPaullinia cupanaSeeds Crude Extract and Ethyl-Acetate Fraction

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Lidiane Vasconcelos do Nascimento Carvalho ◽  
Marina Ferraz Cordeiro ◽  
Thiago Ubiratan Lins e Lins ◽  
Maria Clara Pinheiro Duarte Sampaio ◽  
Gabriela Souto Vieira de Mello ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Biological Activities ◽  
Leukemia Cell ◽  
B Cell Lymphoma ◽  
Ethyl Acetate Fraction ◽  
Leukemia Cell Line ◽  
Breast Adenocarcinoma ◽  
Immunomodulatory Activity ◽  
Native Plant

Paullinia cupana(Guarana) is a native plant of Amazon region that has very traditional importance. Its seeds are rich in bioactive compounds, including tannins, which exhibit relevant properties.Objective.This study aimed to evaluate antibacterial, antineoplastic, and immunomodulatory activity ofP. cupanaseeds crude extract (CE) and ethyl-acetate fraction (EAF).Methods.Antibacterial activity was evaluated by determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antineoplastic activity was evaluated by MTT assays in hepatocellular carcinoma (HepG2), breast adenocarcinoma (MCF-7), ductal carcinoma (T47-D), non-Hodgkin’s B cell lymphoma (Toledo), T cell leukemia (Jukart), and Acute Leukemia (HL-60) cell lines. BALB/c mice splenocytes were treated to assess IFN-γ, IL-6, IL-17, and IL-10 levels by sandwich ELISA.Results.CE and EAF were not toxic to peripheral blood cells and splenocytes. CE and EAF fractions showed a bacteriostatic activity (MIC = 250 μg/mL) and presented IC50values of 70.25 μg/mL and 61.18 μg/mL in HL-60 leukemia cell line. All cytokines evaluated had their levels reduced after treatment, following dose-response model.Discussion and Conclusion.Different biological activities were observed for both CE and EAF, suggestingP. cupanaas a source of bioactive substances, especially tannins that may be used for several diseases treatments.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT AND ANTIDIABETIC POTENTIALS OF DIFFERENT FRACTIONS OF MAYTENUS HEYNEANA ROOT EXTRACT

2019 ◽  
Vol 12 (1) ◽  
pp. 408
Author(s):  
Sumithira G ◽  
Senthil Kumar Gp
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Cation Radical ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Prevention Of Diabetes

Objective: In an attempt to explore herbal drug which may become useful in the prevention of diabetes and antioxidant potential by the ethanol extracts of Maytenus heyneana (MH) root belonging to the family Celastraceae and their different fractions were studied.Methods: Different fractionation was done using chloroform, ethyl acetate, and methanol on ethanolic extract of MH and preliminary phytochemical analysis was done by standard methods to identify the presence of important compounds. In vitro antioxidants activities were carried by 2,2-diphenyl- 2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation radical scavenging assays. For antidiabetic potential, α-amylase and α-glucosidase enzyme inhibitory studies were carried on different fractions.Results: Phytochemical studies show the presence of alkaloids, flavonoids, phenols, cardiac glycosides, and terpenoids in all fractionations; however, tannins and quinones were present in ethyl acetate fraction and saponins in methanolic fraction. For antioxidant activity, ethyl acetate fraction shows concentration of the sample causing 50% inhibition (IC50) values in 22.31 μg/ml and methanolic fraction shows in 12.82 μg/ml concentrations for DPPH and ABTS radical scavenging assay, respectively. In case of antidiabetic activity, methanolic fraction offered significant result in inhibitory action of α-glucosidase and also for α-amylase assay IC50 (5.28 and 3.14 μg/ml) than other fractions.Conclusion: From the results of our studies, it can be concluded that MH shows antidiabetic and antioxidant values and methanolic fraction of MH could be possessed potential constituents in the prevention of diabetes and antioxidant than other fractions. However, further studies are required to validate.

scholarly journals ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus

2020 ◽  
Vol 17 (2) ◽  
pp. 69-75
Author(s):  
Rizky H. Mawardi ◽  
Nanik Sulistyani ◽  
Nurkhasanah Nurkhasanah ◽  
Ricke Desyratnaputri
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Active Fraction ◽  
Methanol Fraction ◽  
Zone Diameter ◽  
Tlc Bioautography ◽  
Muntingia Calabura

This study aims to determine the antibacterial activity and TLC-bioautography profile of the active fractions of Muntingia calabura L. leaves against Staphylococcus aureus. Muntingia calabura L. leaves were macerated with ethanol 96% then fractionated with n-hexane, ethyl acetate, and methanol solvent, respectively. The antibacterial activity was tested by the Kirby-Bauer method to determine the most active fraction and the lowest concentration that inhibited the growth of Staphylococcus aureus. TLC-bioautography was tested using chloroform: ethyl acetate (2:8) as the mobile phase and silica gel F254 as the stationary phase. Antibacterial activity test of n-hexane and ethyl acetate fractions at a concentration of 10% w/v showed activities with inhibition zone diameter of 0.33±0.288 and 9.66±5.77 mm, respectively. At the same time, the methanol fraction showed no activity. The lowest concentration of ethyl acetate fraction which still showed the inhibition zone was 0.312% w/v. The TLC-bioautography profile showed active spots with an Rf value of 0.82 and had an inhibitory zone diameter of 4.013±0.864 mm. It can be concluded that the ethyl acetate fraction was the most active fraction that inhibited the growth of Staphylococcus aureus and had one active spot on the bioautography test.

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