scholarly journals Proximate Analysis and Phytochemical Screening of Triclisia subcordata Oliv Leaf

Author(s):  
F. N. Okpara ◽  
E. O. Nwaichi ◽  
J. O. Akaninwor

Proximate analysis and phytochemical screening of Triclisia subcordata Oliv leaf were investigated using standard analytical methods. Results of the proximate analysis showed contents for moisture (79.28±2.32%), ash (1.91±0.21%), crude fibre (1.83±0.13%), lipid (1.81±0.16%), crude protein (8.32±1.20%) and carbohydrate (6.91±0.74%). Phytochemical screening showed the presence of all six metabolites studied but at different concentrations. Saponin and flavonoids > alkaloids > phenol, tannin and cyanogenic glycoside. Tannins, saponins, alkaloids, flavonoids, cyanogenic glycosides and phenol gave 0.01±0.00%, 5.81±0.23%, 2.32±0.13%, 6.01±0.36%, 0.20±0.00% and 0.03±0.00%. Given observed high contents of moisture, flavonoids and saponins, moderate amounts of alkaloids and low contents of lipid, cyanogenic glycosides, tannins and phenols in Triclisia subcordata Oliv leaf, this study rationalises the medicinal use of the plant, and unveils its potential as a source of micronutrients.

Author(s):  
Adebayo S. Daramola ◽  
Philip O. Amira

This study sought to probe into the health significance of two (white and yellow) species of Dioscorea dumetorum (bitter yam) based on their chemical composition and phytochemicals. Matured tubers were analyzed for proximate composition, mineral contents and phytochemicals, using standard analytical procedures. Significant differences (p<0.05) were observed between the means of some of the compositions in the two species. The results showed moisture contents of 71.45±0.15 and 75.55±1.20 g% wet weight for white and yellow species respectively. Others in g% dry weight included: crude protein (white, 5.20±0.27; yellow, 5.70±0.35), crude ash (white, 4.75±0.16; yellow, 4.05±0.09), crude fat (white, 0.70±0.20; yellow, 0.72±0.01), crude fibre (white, 15.90±0.75; yellow, 14.95±0.070) and carbohydrate (white, 81.86±0.17; yellow, 75.95±0.05). The most predominant minerals were potassium (white, 680±0.20; yellow, 767.85±1.35), phosphorus (white, 270.00±2.10, yellow, 277.25±3.45), sodium (white, 70.35±1.40; yellow, 76.45±0.05) and magnesium (white, 47.10±0.25; yellow, 67.40±0.05). The yellow species showed significantly higher concentrations over the white. The phytochemical screening revealed much presence of glycosides and alkaloids, but little presence of flavonoids, oxalates, phytates and  tannins. The results justify the medicinal use of the tuber and could serve as a synergy to man and animal nutrition.


2020 ◽  
Vol 45 (3) ◽  
Author(s):  
K. O. Soetan ◽  
T. N. Atanda

Lima bean (LB) (Phaseolus lunatus) is an underutilized legumein Nigeria and West Africa despite its numerous nutritional benefits. This study evaluated the proximate composition, phytochemical screening and antinutritional factors (ANFs) of three accessions of Lima beans; LB 001, LB 011 and LB 015 from the International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State. All analyses were done using standard protocols. Statistical analysis was done using descriptive statistics. Result of proximate analysis revealed that LB 011 recorded the highest value for crude protein (25.70 0.06%), crude fat (3.10 0.05%) and crude fibre (3.20 0.06%) and moisture content (9.3 0.16%), LB 015 recorded the highest value for ash (3.90 0.04%), nitrogen free extract (60.35 0.20%) while LB001 gave the highest value for drymatter (92.30 2.50%).Phytochemical screening revealed presence of saponin, flavonoid and alkaloid in the three accessions of LB while anthraquinone and cardiac glycoside were absent in all the LB accessions. Results of ANFs showed that LB 001 recorde the highest value for alkaloid (56.67±5.78mg/g), LB 015 gave the highest value for tannin (93.33±2.89 mg/g) and saponin (61.67±2.89 mg/g) while LB 011 had the highest oxalate content (30±5.00mg/g). The study concluded that the three accessions of lima beans have high levels of crude protein, crude fat and ash and phytochemicals, which compared favourably with that of cowpea (Vigna unguiculata), a conventional legume. However, the ANFs present in LBneed adequate processing for their reduction and optimal utilization as a protein source for human and animals.


Author(s):  
Ezekiel Tagwi Williams ◽  
Nachana’a Timothy ◽  
Attama Chika

This work was designed to explore the phytochemicals, elemental and proximate analysis of Maerua angolensis Stem bark were determined using standard analytical methods. The phytochemical screening showed alkaloid (271.30 mg /100 g), tannins (340.25 mg /100 g), flavonoid (176.85 mg /100 g), reducing sugar (41.20 mg /100 g), glycosides (184.30 mg /100 g), steroids (112.30 mg /100 g), anthraquinones (167.85 mg /100 g) and saponin (225.61 mg /100 g). Also the elemental analysis carried out revealed that the concentration of Manganese (0.02 9 mg/kg), Copper (0.059 mg/kg), Calcium (0.070 mg/kg), Sodium (7.530 mg/kg), Zinc (0.028 mg/kg), Chromium (0.158 mg/kg), Lead (0.007 mg/kg), Iron (0.100 mg/kg) and Magnesium (0.020 mg/kg). The result of the proximate composition showed  that the moisture, fat, crude protein, crude fibre, ash, carbohydrate and energy value content of the samples were 3.58±0.04%, 6.25±0.09%, 21.79±0.26%, 48.51±2.31%, 13.28±1.86%, 6.60±1.79% and 169.81±8.49  kcal/100 g respectively. The pH value obtained was 5.65±0.09. These indicate that the plant can be effective source for drugs. The elemental and proximate analysis shows that it contained appreciable amount of nutrients which could be included in diets to supplement human daily nutrient needs and animal.


Author(s):  
Musbau B. Olaniyi ◽  
Ibraheem O. Lawal ◽  
Aishat A. Olaniyi

Background: One of the uses of Crescentia cujete leaves is in the treatment of cardiovascular diseases, which necessitated the evaluation of its chemical composition.Aim: The aim of the study was to investigate the chemical composition of C. cujete leaves.Setting: Fresh, mature leaves were harvested from the Herbal Garden, Forestry Research Institute of Nigeria, Jericho Hill, Ibadan, Nigeria. The plant samples were identified at Forest Herbarium, Ibadan, Nigeria, sorted to eliminate extraneous matter and then air-dried on a cabinet dryer for five days at ambient temperature and oven-dried at 40°C for five minutes. The dried leaves were milled to powder using a medium-sized milling machine and stored in an air-tight container until needed for analysis.Methods: The proximate composition, phytochemical screening and mineral analysis were determined using standard analytic methods. Minerals such as iron, zinc, calcium, manganese and magnesium were determined by the atomic absorption spectrophotometer; sodium and potassium by the flame photometer and phosphorus by the spectrophotometer.Results: The proximate analysis results revealed that the samples contained carbohydrate (40.46%), crude protein (16.15%), crude fibre (19.71%), crude fat (2.91%), ash (9.77%) and moisture (11.03%), while the phytochemical screening confirmed the presence of cardiac glycosides, saponins, tannins, anthraquinones and flavonoids. The mineral compositions (mg/g) decreased in the order Ca (6.0) > K (5.0) > Mg (2.2) > P (1.5) > Na (0.2) > Fe = Zn (0.6) > Cu = Mn (0.03).Conclusion: These results conceivably indicate that C. cujete is a good source of essential minerals and phyto-nutrients which possess strong pharmacological activities, providing scientific credence for its therapeutic usage in folklore medicine.


Author(s):  
Oyetade Joshua Akinropo ◽  
Bello Lukman Abidemi ◽  
Adesiyan Blessing Adedayo

The proximate analysis of the fruit sample that homogenously sampled from the wild was taken and quartered to get appreciable weight fit for analysis. Due to it perishable nature the quartered sample for each of the fruit was stored in an air tight container and kept in the refrigerator at a temperature of about 4°C. For the determination of the nutritional composition, parameters which include their proximate, minerals, and vitamin C were quantitatively determined while the anti-nutrient composition were qualitatively and quantitatively analyzed. Compared to ackee’s apple, monkey cola was found to consist of 64.41% moisture content, 1.69% ash, 10.21% crude fibre, 1.25% crude fat, 4.44% crude fibre, 18.06% carbohydrates while ackee’s apple consist of 73.21% moisture, 1.49% ash, 9.38 % crude protein, 13.98% crude fat, 2.08% crude fibre, 0.86% carbohydrates and 4.45% of vitamin C. The mineral analysis for both samples was quantitatively determined using Atomic Absorption spectrophotometer (AAS). The minerals determined for ackee’s apple and monkey cola were magnesium which was 1391.65 ppm, calcium 628.23 ppm, sodium 506.96 ppm, potassium 3976.14 ppm, iron 1.0 ppm, copper 5.00, zinc 4.00, cobalt 3.0 and phosphorus 2616.90 ppm the iron however, was not detected. Ackee’s apple on the other hand, consist of magnesium 498.01 ppm, calcium 478.56 ppm, sodium 398.80 ppm, potassium 4970.18 ppm, copper 2.00, zinc 5.00, cobalt 3.0 and phosphorus 373.84 ppm. The qualitative screening of the anti-nutrients revealed the absence of phenol from both fruit samples while tannins were present only in the monkey cola. The flavonoids, phytic acid and oxalate were quantitatively determined to be 1240 mg, 625 mg and 155 mg for Monkey kola and 640 mg, 340 mg and 65 mg for Ackee’s apple.


2016 ◽  
Vol 5 (1) ◽  
pp. 6 ◽  
Author(s):  
Chinyere Ilodibia ◽  
Ngozi Igboabuchi

Phytochemical and nutritional potentials of various parts (leaf, stem and root) of Talinum triangulare were evaluated using standard techniques. Analysis of Variance (ANOVA) was employed in data analysis. All the parts of the species were found to contain nutrient compositions in varying levels. Dry matter and carbohydrate were highest in the stem (91.69±0.12 and 68.66±0.01) respectively. Moisture, ash content and crude fibre were highest in the root (9.78±0.04, 17.53±0.11and 15.32±0.03) respectively while ether extract and crude protein were highest in the leaf (1.09±0.01 and 17.72±0.17) respectively. The results showed also varying quantities of the phytochemicals in the leaf, stem and root with some parts lacking some of the phytochemicals. The leaf contained the highest percentage of all the phytochemical investigated alkaloid, flavonoid, tannin, sterol, Terpenoid, phenol and saponin (0.47±0.01, 0.26±0.00, 0.36±0.07, 0.03±0.01, 0.42±0.02, 0.02±0.01and 0.68±0.05 respectively. The root lacked sterol and phenol while stem lacked only phenol. The leaf, stem and root lacked cyanogenic glycoside. The results have indicated that these parts of the Talinum triangulare investigated are very nutritious and possessed bioactive compounds that could be extracted for the manufacture of new drug, food supplements and also be used in ethnomedicine for the treatment of diseases.


2020 ◽  
Vol 45 (5) ◽  
pp. 92-99
Author(s):  
S. O. Omoikhoje ◽  
D. O. Obasoyo ◽  
S. E. Okosun ◽  
J. I. Uwaya ◽  
I. A. Adamu ◽  
...  

The proximate and phytochemical screening of Senna occidentalis were carried out to assess some nutrients and phytobiotic properties of the leaves. The fresh leaves were thoroughly rinsed, sparsely spread on jute mat and dried at room temperature for 6 – 7 days until they became crispy, thereafter they were milled and subjected to proximate and phytochemical analyses. Data showed that the leaves had low moisture (9.35 9.35%), high crude protein (21.88 21.88%), crude fibre, crude fat, ash and nitrogen free extract (NFE) , of 19.72, 16.88, 9.70 and 22.47% respectively. The phytochemicals positively present were cardiac glycosides, phenols, flavols, flavonols and alkaloids, while saponnins were largely present. From the results, Senna occidentalis leaf meal can be a potential source of vegetable protein and some vital mineral elements as supplements to poultry feeds. Besides, the high levels of antioxidants and antimicrobial agents present in the sample which are known to promote growth by enhancing nutrient utilization, repair of worn out tissues and disease control could make Senna occidentalis leaves worthy of being a phytobiotic additive in poultry diets.


2016 ◽  
Vol 5 (1) ◽  
pp. 97 ◽  
Author(s):  
B. I. Balogun ◽  
S. J. Oniye ◽  
J. Auta ◽  
C. A. M. Lakpini ◽  
F. O. Abeke

This study was conducted to evaluate the suitability of soaked <em>Bauhinia monandra</em> (Kutz) seed as alternative protein source for <em>Clarias gariepinus</em> and to determine cost of feed compounded. The proximate analysis of the diet (crude protein, crude fibre, ash, moisture content and lipid) were determined using AOAC (1980). Data for each parameter was subjected to one way analysis of variance (ANOVA) while means of various results were compared at 5% level of significance. A preliminary study was conducted to determine the best processing method that reduced anti-nutrients to the minimum level without impairing nutrients composition. Boiled, toasted and soaked seeds were used. <em>Bauhinia</em> seeds soaked for 96 hours had least concentration of anti-nutrients. The experimental diets was formulated to contain soaked <em>Bauhinia</em> seed meal (SBSM) at levels of 25%, 50% and 75% inclusion (Diets 2, 3 and 4 respectively)  with two diets acting as control (Diets 1 and 5). All diets were isonitrogenous (40% crude protein) and isocaloric (3212kcal/kg). A 12 weeks feeding trial was conducted using juveniles which were randomly distributed into 12 improvised non-recirculatory and semi-flow through indoor plastic tanks (52cm X 34cm X 33.5cm) at a stocking rate of 10 fish per tank and three (3) replicates per treatment. The experimental design was complete randomized. The fish were fed at 5% body weight, twice daily. Diets with higher inclusion levels of SBSM (diets 3 and 4) significantly depressed growth performance of fish. The variations observed in the Specific Growth Rate (SGR), Feed Conversion Ratio (FCR), Feed Efficiency Ratio (FER) and Protein Efficiency Ratio (PER) were associated with the anti-nutrients present in the diets, these parameters reduced with increasing levels of SBSM in the diets of fish. Based on the relative cost of diets per unit weight gain and protein gain, diet 2 (25% SBSM) was most economical. The results suggested that SBSM can be used to substitute up to 25% levels of dietary protein in <em>C. gariepinus</em> juveniles without significant reduction in growth.


2020 ◽  
Vol 40 (1) ◽  
pp. 161-167
Author(s):  
A. O. Yusuf ◽  
O. S. Sowande ◽  
O. M. Sogunle ◽  
V. A. Akinbami ◽  
O. O. Oyebanji ◽  
...  

Ruminant livestock in most parts of the tropics graze extensively on naturally growing forages which are poor in quality. These tropical forages compared to those in the temperate, support lower levels of ruminant animal production mainly because they contain less nitrogen and are less digestible. The quality and quantity of these grasses become more critical in the dry seasons and thereby imposing more serious constraint to the development and productivity of these animals. Therefore, a study was conducted to access nutritional composition of Panicum maximum and Newbouldia laevis. Phytochemical screening of Newbouldia laevis was also carried out. Newbouldia laevis had higher crude fibre, ether extract and crude protein compared to Panicim maximum. The phytochemical screening revealed the presence of Tannin, Alkaloids, Flavonoids and Saponnin in Newbouldia laevis. The percentages of tannin, alkaloids, flavonoids and saponin in the Newbouldia laevis were 11.5, 52.07, 3.1 and 0.64, respectively while none was recorded for Panicum maximum. The relatively high crude fibre, ether extract and crude protein values for both plants s appeared satisfactory for animal production. Also, the presence of the phytochemicals in the plant showed it importance in ethno veterinary medicine and pharmacology.


Author(s):  
M. N. Abua ◽  
M. A. Ittah ◽  
E. E. Obok ◽  
G. A. Iwo ◽  
R. E. Edugbo ◽  
...  

The present study was aimed to determine the variations in nutritional qualities of 15 mutant lines and two landraces of ginger (Zingiber officinale). Fifteen (15) gamma (γ)-ray induced mutants lines and two landraces of ginger were planted in 2017 early cropping season in the Teaching and Research Farm, Department of Crop Science, Faculty of Agriculture, Forestry and Wildlife Resources Management, University of Calabar, Calabar, Nigeria. To evaluate the nutritional qualities of these seventeen ginger genotypes at maturity, proximate analysis was carried out in the Biochemistry Laboratory of the National Root Crop Research Institute Umudike, Abia State, Nigeria. Using standard and official protocols of the Association of Official Analytical Chemists (AOAC). Results showed that the ginger lines varied significantly (P < 0.01) in all their proximate attributes. The moisture content ranged from 10.13% (UG1) to 12.95% (UG2). Mean dry matter was 88.89%; UG1 and UG2 had the highest (89.89%) and lowest (87.05%) dry matter content, respectively. Mean crude protein was 7.74%; UG2-9-01 and UG2-11-03 had the highest (8.25%) and lowest (7.29%) crude protein respectively. UG1-5-38 and UG1-5-22 had the highest (8.12%) and lowest (6.41%) crude fibre content respectively. The oleoresin content ranged from (6.25%) in UG2-9-01 to (9.09%) in UG1-11-07. UG1-5-04 and UG1-5-22 had the highest (2.88%) and lowest (2.22%) ash content respectively. UG2-9-01 had the highest carbohydrate content of (65.10%). While UG1-5-52 had the lowest (61.27%) The result showed that the ginger lines used in this study had high mean carbohydrate (62.85%) and protein (7.74%) contents as such can be used as supplementary sources of these nutrients for human and livestock. UG1-7-24, UG1-11-07 and UG1-5-18 with high oleoresin contents of 9.11%, 9.09% and 9.05% respectively are recommended to ginger breeders as useful genotypes for improving other ginger lines through micropropagation techniques especially when breeding for oleoresin quality, which is an important quality of ginger. In conclusion, further evaluation and testing of these ginger lines is recommended.


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