scholarly journals GC/MS Analysis of n-Hexane and Chloroform Extracts of Chenopodium murale Leaves in Iraq

Author(s):  
Muthanna Saadi Farhan ◽  
Amjed Haseeb Khamees ◽  
Omar Hussein Ahmed ◽  
Amani AmerTawfeeq ◽  
Yahya Saad Yaseen

Chenopodium murale L. it is an essential annual herbaceous weed belongs to the genus Chenopodium and family Chenopodiaceae. Chenopodium murale L. commonly called as nettle leaf goosefoot. Aim of this study is the gas chromatography-mass spectroscopy analysis of chemical constituents of Chenopodium murale leaves in two different extracts; n-hexane and chloroform. These extracts contain 37 chemical components which are Monoterpenes, steroids precursor and fatty acids. Furthermore the n- hexane extract revealed about 35.22% of cyclic and acyclic monoterpenoids, fatty acid about 2.07%, also 2.31% of nitrogenous compounds and sterol precursor about 0.41%. However the chloroform extract revealed the presence of linolenic acid representing 13.54% and neo menthol representing 18.87%, also the other minor components are carvone oxide (0.27%), alpha- pinene epoxide (1.71%), Trans- Squalene (0.77%) and other minor bioactive components.

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Lobna El-Hosseiny ◽  
Moustafa El-Shenawy ◽  
Medhat Haroun ◽  
Fadhil Abdullah

Gas chromatography/mass spectroscopy analysis was performed to identify the chemical components of three extracted essential oils including thyme, marjoram, and sage. The antibacterial activity of the extracted essential oils against Pseudomonas aeruginosa (ATCC 9027) was investigated using disc diffusion assay, either alone or in combination with standard antibiotics (piperacillin, cefepime, meropenem, gentamicin, and norfloxacin). Results showed that the studied oils exhibited a variety of activities against the tested bacterium. Thyme oil was the most active followed by marjoram oil, whereas sage displayed no activity towards the tested organism. Thyme oil enhanced the antibacterial activity of cell wall targeting antibiotics (piperacillin, cefepime, and meropenem) by more than twofold. Marjoram oil potentiated the activity of all the tested antibiotics except norfloxacin. Sage, despite its inactivity against pseudomonas, synergistically enhanced the activity of piperacillin, meropenem, and gentamicin. Thyme essential oil, containing thymol as a major component (33.6%), exhibited higher activity alone or in combination with antibiotics than marjoram which contained alcoholic terpenes or sage essential oil that contained 1,8-cineole as its major component (29%). The investigated oils, as natural bioactive agents, may be used to enhance the activity of antibiotics towards pseudomonas.


2018 ◽  
Vol 30 (7) ◽  
pp. 1599-1602
Author(s):  
Chandan Krishnamoorthy ◽  
Soumya Krishnan Uma ◽  
Pooja Prafull Jadhav ◽  
Kashifa Ghazal ◽  
Ramalingam Chidambaram

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