Comparative Studies on the Behaviour of Flat Panels Made of GRP Under Static and Dynamic Loads

Glass reinforced plastic, so called GRP, is a composite material made of glass strands called fibbers woven together to create a flexible fabric. GRP is a lightweight material with many and diverse applications ranging from the manufacture of reservoirs for different liquids to the manufacture of boats, yachts, chairs and even children playground furniture. The behaviour of this material under static and dynamic loads is still raising interest from the scientific community and a large number of researchers. This continued interest is due to the material versatility for different applications depending on its manufacture process that has a significant weigh-in in the material mechanical properties. These resulting mechanical properties need to be carefully analysed and benchmarked prior to using the obtained material in commercial applications. The scope of this research study is to analyse the behaviour of glass reinforced plastic plate panel with reinforcements on one and two directions under static and dynamic loads employing both experimental and numerical methods for results validation. The methods used in this research study for the dynamic loads can also be applied successfully to other composite materials. Additionally, the stress plots have been analysed in iteration in order to ensure the most optimal reinforcement pattern.

Study of load bearing capacity of an infinite sheet weakened by multiple collinear straight cracks with coalesced yield zones

This paper is concerned with the analytical solution of a multi-side damage problem. The objective is to investigate the load-bearing capacity of an infinite elastic-plastic plate weakened by three pairs of collinear straight cracks with coalesced yield zones. Stress intensity factors (SIFs) are obtained when yield zones are subjected to three different patterns of yield stress distribution, i. e., constant, linearly, and quadratically varying. Muskhelisvili's complex variable approach is applied for uncovering the solution to this problem. The problem is solved and analyzed rigorously based on Dugdale's hypothesis. The numerical results are deduced for the load-bearing capacity of the plate and yield zone lengths. These results are analyzed and demonstrated graphically for various mechanical loading conditions and different crack lengths.

Exergoeconomic Optimization of Polymeric Heat Exchangers for Geothermal Direct Applications

The highest economic costs of a geothermal plant are basically related to well drilling and heat exchanger maintenance cost due to the chemical aggressiveness of geothermal fluid. The possibility to reduce these costs represents an opportunity to push toward geothermal plants development. Such challenges are even more important in the sites with a low-medium temperature geothermal fluids (90–120 °C) availability, where the use of these fluids for direct thermal uses can be very advantageous. For this reason, in this study, a direct geothermal heating system for a building will be investigated by considering a plastic plate heat exchanger. The choice of a polymeric heat exchanger for this application is upheld by its lower purchase cost and its higher fouling resistance than the common metal heat exchangers, overcoming the economic issues related to conventional geothermal plant. Thus, the plastic plate heat exchanger was, firstly, geometrical and thermodynamical modeled and, after, exergoeconomic optimized. In particular, an exergoeconomic analysis was assessed on the heat exchanger system by using a MATLAB and REFPROP environment, that allows for determination of the exergoeconomic costs of the geothermal fluid extraction, the heat exchanger, and the heating production. A sensitivity analysis was performed to evaluate the effect of main design variable (number of plates/channels) and thermodynamic variable (inlet temperature of geothermal fluid) on yearly exergoeconomic product cost. Then, the proposed methodology was applied to a case study in South of Italy, where a low-medium enthalpy geothermal potential exists. The plate-heat exchanger was used to meet the space heating requests of a single building by the exploitation of low-medium temperature geothermal fluids availability in the selected area. The results show that the inlet temperature of geothermal fluid influences the exergoeconomic cost more than the geometrical parameter. The variation of the exergoeconomic cost of heat exchanger with the inlet geothermal fluid temperature is higher than the change of the exergoeconomic costs associated to wells drilling and pumping with respect to the same variable. This is due the fact that, in the selected zone of South of Italy, it is possible to find geothermal fluid in the temperature range of 90–120 °C, at shallow depth. The product exergoeconomic cost is the lowest when the temperature is higher than 105 °C; thus, the smallest heat exchange area is required. The exergoeconomic optimization determines an optimum solution with a total product cost of 922 €/y for a temperature of geothermal fluid equal to 117 °C and with a number of plates equal to 15.

A Novel ELISA Assay for the Detection of Anti-Prothrombin Antibodies in Antiphospholipid Syndrome Patients at High Risk of Thrombosis

Autoantibodies targeting prothrombin (aPT) can be found in antiphospholipid syndrome (APS) patients. However, their detection has proven difficult to standardize. Here, we developed a new ELISA assay to improve the identification of aPT and compared its performance with currently available anti-phosphatidylserine/prothrombin antibodies (aPS/PT) and autoantibodies targeting prothrombin bound to the plastic plate (aPT-A) assays using a cohort of 27 APS patients at high risk of thrombosis. We generated a novel prothrombin variant, ProTS525A-Biot, carrying an artificial tag at the C-terminus suitable for site-specific biotinylation and added the mutation S525A to improve stability. ProTS525A-Biot was immobilized to neutravidin-coated plates at the desired density and with a defined orientation, i.e., pointing the N-terminal fragment-1 toward the solvent. Antibodies against ProTS525A-Biot (aPT-Bio) were found in 24 out of 27 triple-positive APS patients (88%). When compared to aPS/PT and aPT-A, aPT-Bio showed an excellent linear correlation with aPS/PT (R2 = 0.85) but not with aPT-A (R2 = 0.40). Since aPS/PT but not aPT-A are an emerging biomarker of thrombosis in APS, this method may find utility for detecting pathogenic aPT in APS but also other prothrombotic conditions such as COVID-19.

A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation

The extracellular matrix regulates cell survival, proliferation, and differentiation. In vitro two-dimensional cell experiments are typically performed on a plastic plate or a substrate of a single extracellular matrix constituent such as collagen or calcium phosphate. As these approaches do not include extracellular matrix proteins or growth factors, they fail to mimic a complex cell microenvironment. The cell-derived matrix is an alternative platform for better representing the in vivo microenvironment in vitro. Standard decellularization of a cell-derived matrix is achieved by combining chemical and physical methods. In this study, we compared the decellularization efficacy of several methods: ammonium hydroxide, sodium dodecyl sulfate (SDS), or Triton X-100 with cold or heat treatment on a matrix of Saos-2 cells. We found that the protocols containing SDS were cytotoxic during recellularization. Heat treatment at 47 °C was not cytotoxic, removed cellular constituents, inactivated alkaline phosphatase activity, and maintained the levels of calcium deposition. Subsequently, we investigated the differentiation efficiency of a direct bone coculture system in the established decellularized Saos-2 matrix, an inorganic matrix of calcium phosphate, and a plastic plate as a control. We found that the decellularized Saos-2 cell matrix obtained by heat treatment at 47 °C enhanced osteoclast differentiation and matrix mineralization better than the inorganic matrix and the control. This simple and low-cost method allows us to create a Saos-2 decellularized matrix that can be used as an in vivo-like support for the growth and differentiation of bone cells.

Aptamer BC 007’s Affinity to Specific and Less-Specific Anti-SARS-CoV-2 Neutralizing Antibodies

COVID-19 is a pandemic respiratory disease that is caused by the highly infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Anti-SARS-CoV-2 antibodies are essential weapons that a patient with COVID-19 has to combat the disease. When now repurposing a drug, namely an aptamer that interacts with SARS-CoV-2 proteins for COVID-19 treatment (BC 007), which is, however, a neutralizer of pathogenic autoantibodies in its original indication, the possibility of also binding and neutralizing anti-SARS-CoV-2 antibodies must be considered. Here, the highly specific virus-neutralizing antibodies have to be distinguished from the ones that also show cross-reactivity to tissues. The last-mentioned could be the origin of the widely reported SARS-CoV-2-induced autoimmunity, which should also become a target of therapy. We, therefore, used enzyme-linked immunosorbent assay (ELISA) technology to assess the binding of well-characterized publicly accessible anti-SARS-CoV-2 antibodies (CV07-209 and CV07-270) with BC 007. Nuclear magnetic resonance spectroscopy, isothermal calorimetric titration, and circular dichroism spectroscopy were additionally used to test the binding of BC 007 to DNA-binding sequence segments of these antibodies. BC 007 did not bind to the highly specific neutralizing anti-SARS-CoV-2 antibody but did bind to the less specific one. This, however, was a lot less compared to an autoantibody of its original indication (14.2%, range 11.0–21.5%). It was also interesting to see that the less-specific anti-SARS-CoV-2 antibody also showed a high background signal in the ELISA (binding on NeutrAvidin-coated or activated but noncoated plastic plate). These initial experiments suggest that the risk of binding and neutralizing highly specific anti-SARS CoV-2 antibodies by BC 007 should be low.

Moldability improvement in microinjection molding via film lamination

The purpose of this study is to integrate a polymeric film onto a mold to impede thermal heat transfer during resin infusion. A thin plastic plate was fabricated by using microinjection molding. A polyimide (PI) film was laminated onto a mold in an effort to produce a thin light guide plate (LGP). The film could decelerate the solidification of molten polymer in the cavity of mold and enhance the wall slip of resin on the mold. The insulation effect was modeled numerically. The surface roughness and pattern transfer characteristics of the LGP were evaluated. It was found that the fluidity of the resin increased due to the decreased skin layer during mold filling. The results showed that the strategy proposed in this study could help decrease the thickness of LGP effectively when manufacturing the part via injection molding.

IMPACT OF INTERLAMINAR ELLIPTICAL DEFECTS UPON BEHAVIOR OF RECTANGULAR CARBON PLASTIC PLATE AT STATIC AND DYNAMIC LOADS

The purpose of this work is to investigate the impact of the inner defects of elliptical stratification type upon behavior of the rectangular carbon plastic plate at the impact of static and dynamic loads. The investigation methods: the problem is solved in a numerical way with the aid of a finite ele-ment method (FEM) in the LS-DYNA software com-plex (Livermore Software Technology Corp.). The investigation results: the distribution of stresses in plate layers under the impact of static and dynamic loads is obtained. The distribution of destruc-tion indices with the use of different destruction criteria for unidirectional composites (on the basis of carbon band) is defined. Conclusions: the impact of defects of the type of specified shape stratification, dimensions, amount and places of location with regard to the plate under consideration under the action of compressive static load does not practically tell. Under the action of the compressive dynamic load there is observed a noticeable impact of inner defects upon rectangular plate behavior.