time of death
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2021 ◽  
Vol 2 (1) ◽  
pp. 7-19
Author(s):  
Timothy P. Gocha ◽  
Sophia R. Mavroudas ◽  
Daniel J. Wescott

The Forensic Anthropology Center at Texas State (FACTS) began accepting whole-body donations for scientific research and educational purposes under the Texas Anatomical Gift Act in 2008. Research conducted with donated whole bodies involves studies in taphonomy and human decomposition, including reconstructing the postmortem interval. Following decomposition, the skeletal elements of all donors are collected, cleaned, and permanently curated into the Texas State Donated Skeletal Collection (TXSTDSC), which is used for teaching and research by faculty and students at Texas State but is also open to external researchers. To date, FACTS has received 710 donors. Fifty-eight percent of donors are male and 42% are female. Donor ages range from 21 weeks’ gestation to 103 years old at the time of death, with a mean of 66 years, and a median of 68 years. Based on self-identified or family-identified ancestry, 90% of donors are White, 4.5% are Hispanic, 3% are Black, less than 2% are of mixed ancestry, and less than 1% are Asian or Native American. Information collected about each donor includes geographic/residential history; occupational history; socioeconomic status; anthropometrics; parity status; alcohol, tobacco, and drug use history; mobility status; an overall health questionnaire; cause and manner of death.


2021 ◽  
Vol 23 (2) ◽  
pp. 98-105
Author(s):  
Edo Legianto Pratama ◽  
Tuti Heiriyani ◽  
Riza Adrianoor Saputra

[JATROPHA SEED POTENTIAL AS A NATURAL RODENTICIDE]. The controlling of rat's pest attacks often use chemical control that adversely affects non-target animals and leaves a residue for the environment. One of the recommended controls is to use natural rodenticides derived from jatropha seeds and do not harm non-target animals that are environmentally friendly. This research aims to determine the interaction and the best combination of bait mixed with jatropha seeds on the death of Mus musculus, as well as the type of bait and the best dose of jatropha seeds on the death of Mus musculus. The design in this study used a completely randomized design consisting of two factors, the first factor was the bait and the second factor was the dose of jatropha seeds. The results showed that there was an interaction between the type of bait and the dose of jatropha seeds on the amount of feed consumed by Mus musculus. The combination of bait type and dose of jatropha seeds that was best for the mortality of Mus musculus was found in the treatment of rice flour with 3.0 g of jatropha seeds, cornflour with 3.0 g of jatropha seeds, and fish meal with 3.0 g of jatropha seeds. The best dose of jatropha seeds in reducing the bodyweight of Mus musculus, accelerating the time of death, and increasing the percentage of death was 3.0 g of jatropha seeds with an average decrease in body weight of Mus musculus reaching 75%, and the fastest death for five days, and able to kill 100% of Mus musculus.


Author(s):  
Siti Eliza Hasningsih ◽  
Niken Satuti Nur Handayani ◽  
Putut Tjahjo Widodo

Time of death (TOD) determination is crucial in criminal cases. The method used to determine the TOD so far is only based on the state of the corpse found, therefore a new method is needed to improve the accuracy of the TOD determination. This study aims to determine the TOD based on a new method, namely the formation of blood rings and RNA degradation. Blood is commonly found in crime scenes. Blood consists of liquid part that is plasma and cellular part consisting of erythrocytes, leukocyte, and thrombocytes. The composition of blood as a liquid that contains dissolved solids makes the drops of dried blood forming “coffee ring effect”, which is a ring-like form on the perimeter of a blood drop. Coffee ring effect is used as an indicator of time by looking at the thickness of the ring formed from the perimeter of the blood drop to the middle which increases with time. RNA degradation was observed using Peptidylprolyl isomerase A (PPIA) gene. The PPIA gene is found in leukocyte and is used to see the degradation of RNA per 30 minutes period using the RT-PCR and qPCR methods. Degradation was observed by comparing the cycle threshold (ct) value of the standard curve with the ct value of the samples per unit time. TOD could be determined by the percentage of the blood ring thickness up until 120th minutes, and by observing the degradation of RNA until the 60th minute, after that the RNA had completely degraded.


2021 ◽  
Vol 8 (3) ◽  
pp. 177-181
Author(s):  
E. Grygorian ◽  
V. Olkhovsky ◽  
M. Gubin ◽  
V. Shishkin

Purpose: The postmortem interval (PMI) evaluation is one of priorities while performing a forensic medical examination of corpse. To date, there is lack of information of morphological postmortem changes of some internal organs. Considering the persistent need to develop the method for a precise assessment of PMI, postmortem changes in these potentially informative organs were evaluated. The aim of study was to analyze morphological postmortem changes in prostate and uterus. Materials and Methods: histological samples of 40 prostate tissues and 40 uterus (n=80) from corpses of deceased aged 18-75 years. Only cases with known time of death were included to study, the time of death was taken from police reports. Exclusion criteria were cases of violent death, cases of death with massive blood loss, tumors of studied internal organs, cases when diagnosis was not made by a forensic medical examiner. The PMI of studied cases ranged from 1 to 6 days. Histological slides were made with a staining by hematoxylin and eosin, x200 magnification, using Olympus ВХ41 and Olympus ВХ46 microscopes, Olympus SC50 camera. Postmortem morphological changes were evaluated by a calculation of blank spaces percentage in microscopical structures using a JS-based program. Connection between PMI and morphological changes was calculated by the Spearman’s rank correlation. Results: the average percentage of blank spaces in uterus tissues was smaller than in prostate tissues (1,99 and 9,65 relatively). The slower growing of blank spaces was in uterus. In prostate samples, a notable increase of blank spaces was observed between 48 and 72 hours after the death. After this period, the increase slowed down and then an increase was observed again between 120 and 144 hours after the death. In uterus samples, a slight acceleration observed between 72 and 120 hours after the death and then slowing down between 120 and 144 hours after the death. Blank spaces in evaluated histological slides were increasing directly proportional to the PMI, a statistically significant interconnection was defined (p < 0.05). Conclusions: The morphological postmortem changes in prostate and uterus were developing at certain time frames. Blank spaces percentage, in studied histological slides, were increasing directly proportional to the PMI increase, a statistically significant interconnection was defined. Therefore, the results of study show the possibility of the evaluation of a postmortem time interval by assessing such morphological changes in these organs, which could be used in forensic medical cases.


Metabolites ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 801
Author(s):  
Joanna Dawidowska ◽  
Marta Krzyżanowska ◽  
Michał Jan Markuszewski ◽  
Michał Kaliszan

Recently, the diagnostic methods used by scientists in forensic examinations have enormously expanded. Metabolomics provides an important contribution to analytical method development. The main purpose of this review was to investigate and summarize the most recent applications of metabolomics in forensic science. The primary research method was an extensive review of available international literature in PubMed. The keywords “forensic” and “metabolomics” were used as search criteria for the PubMed database scan. Most authors emphasized the analysis of different biological sample types using chromatography methods. The presented review is a summary of recently published implementations of metabolomics in forensic science and types of biological material used and techniques applied. Possible opportunities for valuable metabolomics’ applications are discussed to emphasize the essential necessities resulting in numerous nontargeted metabolomics’ assays.


Author(s):  
Yuli Wahyu Tri Mulyani ◽  
Siti Nurjanah ◽  
Samsuar Samsuar ◽  
Laila Susanti

<p class="Judul1"><span lang="EN-US">Pediculosis is a disease caused by infestation of head lice (<em>Pediculus humanus capitis</em>) which is a blood-sucking obligate ectoparasite. The essential oil of durian fruit peel has active compounds that have the potential as vegetable pediculosides that can kill head lice. This study aimed to find the best concentration <span>of </span>essential oil from durian fruit peels that can kill head lice. The research design used with an essential oil concentration of 100%, 75%, 50%, 25%, distilled water as negative control and permethrin 1% as positive control, three repetitions and each concentration consisting of 10 flea tails. The parameters observed were the condition of lice morphologically based on the activity of limb movement, antenna movement and digestion, then the calculation of mortality was observed based on the time of the test. The results showed that the concentration of durian peel essential oil as an antipediculosis had a significant effect on the time of death of head lice, limb movement and digestion. The test result showed that the concentration is 100%, has a death time of 12 minutes <span>32 </span>seconds, 75% concentration was 16 minutes 28 seconds, 50% concentration was 21 minutes 11 seconds, 25% concentration was 32 minutes 23 seconds, negative control was 61 minutes 15 seconds. The fastest time of death in positive control for chemical pediculosides was 10 minutes 15 seconds. The conclusion in this study was that essential oils the dominan one is 1,5-naphthyridin-2-amine were proven to kill head lice and the best concentration was close to positive control, namely at a concentration of 100%.</span></p>


2021 ◽  
Vol 16 (3) ◽  
Author(s):  
Branislav Anđelković ◽  
Jonathan P. Elias

An anthropoid wooden coffin with human mummy was purchased in Luxor in February 1888 by the Serbian mécène and world traveler Pavle Riđički (1805‒1893). Due to historical, political and cultural circumstances the first studies of the mummy did not start until May 1993. The ancient ‘patient’ ‒ Nesmin, stolist-priest of Akhmim, son of Djedhor (son of Wennefer, son of Djedhor) born to Chay-Hathor-Imw/Tjay-Hathor-imw ‒ who became known as the Belgrade mummy ‒ underwent a CT scan at the University of Belgrade, Faculty of Dental Medicine, Diagnostic Radiology Center. The present paper provides the first complete analysis of the CT scan. At the time of death (350‒325 B.C.) Belgrade Nesmin was between 35 and 40 years old. A proper bioanthropological study is presented. The mummification features are discussed. The distribution of funerary amulets on the mummy has been established. The mummy’s cultural biography is specified. A museum superstition phenomenon is noted.


Author(s):  
Arooj Farooq

Introduction: Postmortem interval (PMI) is the time lapse between death of a person and its postmortem examination i.e. autopsy. Estimating the postmortem interval (PMI) is an imperative perspective of forensic medicine. Aims & Objectives: This study was conducted to see the impact of CSF protein estimation on determination of PMI. Place and duration of study: It was an observational correlational study, conducted for one year at Department of Forensic Medicine, King Edward Medical University Lahore. Material & Methods: A total of 119 cadavers were included in this study. Chemicals used were disodium molybdate, pyrogallol, succinic acid. The minimum detectable concentration of total proteins in CSF with dye binding method using pyrogallol red was determined as 0.022g/l. Two ml of CSF was taken from each cadaver. Clear, colorless samples were taken in test tubes. Turbid and blood contained samples were not included in study. Protein concentration was determined using photospectrometry. Statistical analysis was done by SPSS-23. Quantitative variables like age were presented as mean ± SD. Qualitative variables like gender were presented as frequency and percentages. For comparison between PMI and CSF proteins concentration correlation was applied. Results: Males accounted for majority of our subjects. The mean value of proteins in CSF was 219.91± 113.121 mg/dl. The most common PMI was 11 to 20 hours. CSF proteins increased gradually over 72 hours after death. The results of present study showed a significant positive correlation between time of death and CSF proteins concentration after death. Conclusion: It is observed that as a whole protein concentration increased with increasing time of death. Hence, CSF protein concentration can be used in estimating time since death.


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