Trinexapac-ethyl application time in the crop corn agronomic performance grown under different plant arrangements

Reduced row spacing promotes more uniform spatial distribution of plants in the field. However, the adoption of reduced row spacing only is possible with smaller plants, which may be obtained with the use of plant growth regulator. This study aimed to evaluate the agronomic performance of the first corn crop with Trinexapac-ethyl applied at the different plant development stages and grown under different row spacing, with the same plant population. The experiments were arranged in a split-plot randomized block design with four replications, with row spacing for the plots (0.45 and 0.90 m) and Trinexapac-ethyl (TE) application time to subplots (control without application, at the V3, V6, V9 and V12 phenological stages). The Trinexapacethyl application time interacted with row spacings changing the growth and yield performance of the corn crop. For 0.45 m spacing Trinexapac-ethyl application at V12 and for 0.90 m spacing application at V9 and V12 reduced plant height and ear height. Trinexapac-ethyl application at V9 for both row spacings changed the plant architecture without changing the ear length and grain yield.

The Physicochemical, Microbiological, and Structural Changes in Beef Are Dependent on the Ultrasound System, Time, and One-Side Exposition

The effect of high-intensity ultrasound (HIU) system (bath, 37 kHz and 90 W/cm2; or probe, 24 kHz and 400 W) and application time (25 or 50 min, one-side exposition) on the properties of bovine Longissimus lumborum after 7 d of storage at 4 °C was studied. The bath system significantly increased the lightness of the muscle, while other color parameters (a*, b*, hue, and chroma) were not different from the control. The water holding capacity and shear force decreased significantly (3.1–5% and 0.59–0.72 kgf, respectively) in sonicated meat independently of the system, favoring the tenderization of the muscle after storage. Microstructural changes observed in the HIU-exposed surface provided evidence of a higher area of interfibrillar spaces (1813 vs. 705 µm2 in the control), producing tenderization of the muscle, compared with the control. HIU significantly increased counts of total aerobic and coliform bacteria, especially after 50 min of ultrasonication. HIU also increased lactic acid bacterial counts in the bath system. Single-sided muscle exposition to ultrasound may produce sufficient significant changes in muscle properties, which could decrease long treatment times that would be needed for the exposition of both sides. HIU in bath systems increases tenderness by modifying meat ultrastructure, with no significant changes in physicochemical parameters. Nevertheless, microbiological quality may need to be considered during the process due to a slight increase in bacterial counts.

Study of Trichoderma Spp. Application on the Incidence of Moler Disease and Shallot's Growth and Yield

Fusarium wilt disease/moler is a concern in onion cultivation. Many farmers apply chemical pesticides such as azoxystrobin and difenoconazole to control the diseases. Both of these chemical pesticides are currently exhibiting a decline in effectiveness, prompting farmers to increase the dosage and frequency of application. Recommendations for biological control, including Trichoderma spp.. This study aimed to determine the influence of application time of of Trichoderma spp. in various media types on the incidence of moler disease and shallot growth and yield. This study was conducted in Tabalong district, South Kalimantan, from February to June 2019 using a completely randomized design (CRD) factorial comprising two nested designs. The first factor was the type of media in which Trichoderma spp.. was applied, namely solid and liquid. The second factor was the application time of Trichoderma spp.. Level 1 was the application of Fusarium sp. seven days before planting and Trichoderma spp.. seven days after planting. Level 2 included the application of Trichoderma spp.. seven days before planting and Fusarium sp. simultaneously during planting. Level 3 involved the administration of Trichoderma spp.. concurrently with planting and Fusarium sp. seven days later. This investigation used one positive control (without treatment) and one negative control (shallots inoculated with Fusarium). Each experimental unit was consisted of ten plants and replicated three times. The parameters observed were the incubation period of Fusarium sp., disease incidence, number of leaves, plant height, number of cloves, and average clove weight. The study found that the application time of Trichoderma on various types of media affected the incidence of Fusarium disease, the number of leaves, and the number of shallot cloves.When compared to liquid media, the application of solid media resulted in a reduced incidence of Fusarium disease and a greater frequency of flares. The application time on solid media gave a low response to moles. As for liquid media, the lowest incidence of moler disease occurred at the seven days before planting.

Effectiveness of ACB-IP 1.0 Universal Pathogen Free Concentrated Cocktail Convalescent Plasma in COVID-19 Infection

BACKGROUNDThe efficacy of SARS-CoV2 single donor convalescent plasma (CP) varied according to the application time and the amount of antibody that is administered. Single donor CP has some drawbacks; such as the insufficient levels of neutralizing antibody activities, the requirements of blood group compatibility, and the risk of infection transmission. In this study, the safety and efficacy of pathogen inactivated, isohemagglutinin-depleted (concentrated) and pooled CP product was investigated. MATERIALS AND METHODSA total of sixteen patients were treated with either single donor CP (N:9) or pathogen-free, concentrated, pooled CP (ACB-IP 1.0) (N:7).RESULTSFive out of six single donor plasma SARS-CoV2 antibody titers remained below 12 s/co, but the antibody titers of all ACB-IP 1.0 plasma were above 12 s/co. SARS-CoV2 total antibody titers of ACB-IP 1.0 plasma were statistically higher than the antibody titers of single donor CP. Mean total plasma neutralizing antibody activity of ACB-IP 1.0 plasma (1.5421) was found statistically higher than single donor CP (0.9642) in 1:256 dilution (ρ<0.01)The mortality rates of the patients treated with ACB-IP 1.0 plasma were statistically lower (p< 0.05) than the patients treated with single donor CP. The administration of either single donor CP or ACB-IP 1.0 plasma to the patients within eight days significantly shortened the length of hospitalization (ρ< 0.01).CONCLUSIONThe present study established ACB-IP 1.0 plasma product as a safe and potentially effective treatment for COVID-19, allowing rapid access to patients in need.TRIAL REGISTRATIONTrial Registration Number: NCT04769245Trial Registration Date: 17.03.2021

Effect of Exposure Times of Sodium Hypochlorite before Acid Etching on the Microshear Bond Strength to Fluorotic Enamel

Purpose: To evaluate the effects of different treatment time of 5.25% Sodium hypochlorite (NaOCl) on the microshear bond strength (μSBS), attenuated total reflection Fourier transform infrared (ATR-FTIR) and etching pattern in mild and moderate fluorotic enamel. Study design: Forty-eight fluorotic molars were divided into two groups: mild and moderate fluorotic enamel which were classified by a Thylstrup and Fejerskov index (TFI). Based on the application time (0s, 60s, 120s, 180s) of 5.25% NaOCl, each group was sectioned into four parts. Then the etched enamel was bonded with resin and tested to acquire μSBS. The statistical method was two-way ANOVA and Least Significant Difference (LSD) test at α = 0.05. Besides, fracture modes were observed under a stereo microscope. SEM was used to evaluated the enamel-etching pattern and organic content on the fluorotic enamel surface were investigated by ATR-FTIR. Results: Duration of 5.25% NaOCl at 60s or 120s significantly increased the μSBS of fluorotic enamel compared to 0s (p&lt;0.05). Fracture modes indicated that dominating failures were set in the bonding interface but whose proportion decreased when 5.25% NaOCl was applied. The enamel-etching pattern in 180s was deepest under SEM. Spectra of enamel samples manifested an obvious and gradual removal of its organic phase after duration of NaOCl increased. Conclusion: The maximal μSBS is acquired by using 5.25% NaOCl at 60s for mild fluorotic enamel but 120s for the moderate. The prolonged application time of 5.25% NaOCl prior to phosphoric acid etching improves enamel-etching pattern. Treatment of 5.25% NaOCl decreases proteins on the fluorotic enamel surface.