Characteristics Environmental and Anopheles Larva Species In High And Low Clinical Malaria Cases In The Landak District of West Kalimantan Province

Malaria remains a health problem in Indonesia. West Kalimantan is a malaria endemic area with high and lowincidence. Landak District is one of the malaria endemic area. Malaria cases were found in the areas around illegalgold mining and oil palm plantations. The aims of this study were to describe the characteristics of the breeding sitesand species of Anopheles larvae found in high malaria cases area, namely Amboyo Utara Village and low clinicalmalaria cases, area namely Mandor Village. This research is a descriptive research with cross sectional design. Thesamples were Anopheles larvae collected with Accidental sampling technique in the breeding sites. Environmentalcharacterization of breeding sites were physical characteristic including water temperature and sun exposure,chemical characteristic including water pH and salinity, and biological characteristics including water biota. Theresults of this study ware environmental characteristics that have the potential to breed Anopheles mosquitoes inAmboyo Utara Village, including water temperature 26-30C, shandy, water pH 5.0-7.6, salinity 0.2-1.0 ppt, biotaswater hyacinth, grass and tadpole. The Mandor village, water temperature 29-30 ºC, shandy, pH of 6.9-8.0, salinity of0.5 ppt, water biota grass. Anopheles species found in Amboyo Utara village were larvae of An. vagus (94.30%), An.tessellatus (3.42%), An. subpictus (1.62%), An. indefinitus (0.81%) and An. maculatus (0.81%). Characteristics ofbreeding sites in Mandor village were larvae of An. maculatus (11.11%), An. subpictus (3.70%), and An. vagus(85.18%). The conclusion of this study was that di erent species found at breeding sites with di erent environmentalcharacteristics in both high and low malaria areas in Landak District, West Kalimantan Province.

The Comparative Effectiveness of Adult Mosquito Sampling Methods Dealing With Odor-baited Resting Box Traps in a Malaria-endemic Area in Southern Iran

BackgroundIran is under threat of a potential outbreak of mosquito-borne diseases, such as malaria, Dengue fever, Chikungunya, and Zika. The study aimed to determine the efficiency and sustainability of some adult mosquito sampling methods for designing effective entomological surveillance systems in a malaria endemic area.MethodsDifferent rates of tap water, sugar, and yeast (Saccharomyces cerevisiae) were mixed to provide CO2. Anopheles stephensi and Culex pipiens Bandar Abbas strains were reared in the insectary and used for tests. On a lab scale, CO2 orientation experiments were performed using a Y-tube olfactometer on the insectary mosquito strains. In the field trial, human landing catches (HLC), artificial pit shelter (APS), CO2-baited trap (CO2-BT), human and cow odor baited resting boxes (HOBT, COBT), cow urine baited trap (CUBT), and colored un-baited box (UB) were studied in Bandar Abbas, Hormozgan Province, south of Iran. Mean densities of An. stephensi and Cx. pipiens insectary strains, which oriented to CO2 as flowrate of 170, and 300 mL/minute was significantly higher compared to the control group (P < 0.05). The black un-baited inbox resting traps significantly more collected wild mosquito species compared to others colored UB (P ˂ 0.05). ResultsA total of 2722 collected mosquitoes comprises Culex pipiens (48.56%), Anopheles fluviatilis s.l.(14.21%), An. stephensi (11.68%), Cx. theileri (9.95%), Aedes caspius (7.01%), An. dthali (6.79%) An. culicifacies s.l. , An. pulcherrimus, An. sergentii,An. superpictus s.l., Cx. sitiens and, Ae.caspius less than 1%. Anopheles stephensi and, An.sergenti were most collected in CUBT. Anopheles fluviatilis s.l. and Ae. caspius were most found in HLC. Anopheles dthali, Cx. pipiens and, Cx. theileri were most abundant in APS. ConclusionsBlack CUBT and APS methods can be suggested as a perfect sampling strategy for malaria vectors surveillance. APS, and HLC methods were found useful to entomological surveillance systems for arboviral and filarial vector-borne diseases. Further modified sampling methods should be devoted to identify more effective sampling methods.

The Comparative Effectiveness of Adult Mosquito Sampling Methods Dealing With Odor-Baited Resting Box Traps in A Malaria-Endemic Area in Southern Iran

Background Iran is under threat of a potential outbreak of mosquito-borne diseases, such as malaria, Dengue fever, Chikungunya, and Zika. The study aimed to determine the efficiency and sustainability of some adult mosquito sampling methods for designing effective entomological surveillance systems in a malaria endemic area. Methods Different rates of tap water, sugar, and yeast (Saccharomyces cerevisiae) were mixed to provide CO2. Anopheles stephensi and Culex pipiens Bandar Abbas strains were reared in the insectary and used for tests. On a lab scale, CO2 orientation experiments were performed using a Y-tube olfactometer on the insectary mosquito strains. In the field trial, human landing catches (HLC), artificial pit shelter (APS), CO2-baited trap (CO2-BT), human and cow odor baited resting boxes (HOBT, COBT), cow urine baited trap (CUBT), and colored un-baited box (UB) were studied in Bandar Abbas, Hormozgan Province, south of Iran. Mean densities of An. stephensi and Cx. pipiens insectary strains, which oriented to CO2 as flowrate of 170, and 300 mL/minute was significantly higher compared to the control group (P < 0.05). The black un-baited inbox resting traps significantly more collected wild mosquito species compared to others colored UB (P ˂ 0.05). Results A total of 2722 collected mosquitoes comprises Culex pipiens (48.56%), Anopheles fluviatilis s.l.(14.21%), An. stephensi (11.68%), Cx. theileri (9.95%), Aedes caspius (7.01%), An. dthali (6.79%) An. culicifacies s.l., An. pulcherrimus, An. sergentii,An. superpictus s.l., Cx. sitiens and, Ae.caspius less than 1%. Anopheles stephensi and, An.sergenti were most collected in CUBT. Anopheles fluviatilis s.l. and Ae. caspius were most found in HLC. Anopheles dthali, Cx. pipiens and, Cx. theileri were most abundant in APS. Conclusions Black CUBT and APS methods can be suggested as a perfect sampling strategy for malaria vectors surveillance. APS, and HLC methods were found useful to entomological surveillance systems for arboviral and filarial vector-borne diseases. Further modified sampling methods should be devoted to identify more effective sampling methods.

EFEKTIVITAS EKSTRAK KULIT BATANG MAJA (Aegle marmelos L) TERHADAP KEMATIAN LARVA NYAMUK Anopheles sp.

<em>Malaria is one of the public health problems that can cause death in infants, children under five, pregnant women and reduces work productivity. Vector control by chemical means leads to vector resistance and environmental pollution and negatively impacts public and animal health. This study aims to determine the effectiveness of the bark extract of Maja (Aegle marmelos L) against the death of Anopheles sp mosquito larvae with concentrations of 5%, 10%, 20%, 40%, and 50% and the observation of contact times every 15 minutes, 30 minutes, 60 minutes, 90 minutes and 120 minutes. The study used a Completely Randomized Design (CRD) with Factorial. Extraction used maceration method, with 70% ethanol as solvent. Larvae of Anopheles sp. instar III obtained from malaria endemic area. The test used a 200 ml container, with two replications. All data were analyzed by two way ANOVA and Tukey HSD test. This study obtained mortality of 93.3% larvae at a concentration of 50% and a contact time of 120 minutes. The results of statistical analysis showed a significant effect of concentration (p=0.0001), contact time (p=0.0001), and interaction concentration-contact time (p=0.004) on the mortality of Anopheles sp. mosquito larvae. This study has proven the use of bark extract of Maja (Aegle marmelos L) as an alternative bio-insecticide in controlling mosquito larvae of Anopheles sp.</em>

Mixed Plasmodium Malariae Infections Were Underdetected in a Malaria Endemic Area in the Amazon Region, Brazil

Plasmodium malariae infections are often asymptomatic and long-lasting. Mixed infections are often underdetected in areas where P. malariae, P. vivax, and P. falciparum are coendemic. In this study, we described the occurrence of these species circulating as single or mixed infections in Pará state, Brazil, in the Amazon region, with the purpose of clarifying the impact of misidentification of parasite species based only on morphological description using thick blood smear. By using real-time polymerase chain reaction based on the amplification of the mitochondrial DNA, we detected a prevalence of 46% (58/126) mixed infections with 33.3% P. malariae/P. vivax which were read as P. vivax monoinfections by microscopy detection. Our findings confirmed the high circulation of P. malariae in a malaria endemic area in the Brazilian Amazon region.

Application of High-Resolution Melting (HRM) Technique Towards the Detection of Asymptomatic Malaria in a Malaria Endemic Area of Southeastern Iran Under Elimination Program

Background: Asymptomatic malaria, which usually exists in low parasitemia, acts as the Plasmodium species reservoirs contributing towards malaria transmission. This situation hinders malaria elimination programs in endemic areas, thus necessitating an active case detection with a high sensitive method and treatment of cases. This is why we used a High Resolution Melting (HRM) assay to monitor the trend of asymptomatic malaria in a malaria endemic area of Iran which is under elimination program. Methods: The peripheral blood was sampled from 271 clinically approved non-febrile individuals from a malaria endemic zone of southeastern Iran for asymptomatic malaria prevalence detection by microscopy, Rapid Diagnostic Tests (RDTs) and HRM methods. The HRM assay was done based on the amplification of 18S SSU rRNA gene. Results: The HRM assay revealed infections from three individuals out of 271 (1.1% asymptomatic malaria prevalence) from the participants, two Iranian natives with Plasmodium vivax infection and one Pakistani immigrant with P. falciparum infection. Neither microscopy nor RDTs detected Plasmodium spp infections from the 271 non-febrile individuals. The nucleotide sequencing analysis of the positive controls used in this study showed a close homology with the reference gene bank sequences of P. falciparum 3D7 (CPO16995.1) and P. vivax Sal-1(UO3079.1). Conclusion: This study revealed a low frequency of asymptomatic malaria trend within malaria endemic areas of southeastern Iran which are under intense elimination program and also the ability of HRM assay in detecting low Plasmodium spp parasitemia beyond the limits of microscopy and RDTs.

Prevalansi Malaria di Puskesmas Sanggeng Kabupaten Manokwari Periode November sampai Desember 2019

Malaria is a disease caused by parasitic infection, named Protozoa from the genus Plasmodium which is transmitted to humans by the bite of Anopheles mosquito. Manokwari Regency, which is located in West Papua Province, is a high malaria endemic area with Annual Parasite Incidence (API) 22.88 in 2018, this numbers is included in the High Case Incidence (HCI)> 5 category. The aim of this study is to analyze malaria cases in patients who treated at Sanggeng Public Health Center from November to December 2019 based on; the number of cases, patient characteristics (age group and gender) and type of Plasmodium. This research method is descriptive with a laboratory approach, namely microscopic examination of thin and thick blood preparations using a microscope. The results of the study of 730 patients, there were 35 malaria positive blood supplies. The highest prevalence of people with malaria were aged ≥ 15 years (51.42%). Most of the patients with malaria based on gender were women (51.43%) and the types of plasmodium found were Plasmodium falciparum (20%) and Plasmodium vivax (80%). Keywords: Plasmodium falciparum, Plasmodium vivax, Anopheles, malaria endemic ABSTRAK Malaria merupakan penyakit yang disebabkan infeksi parasit yaitu Protozoa dari genus Plasmodium yang ditular pada manusia oleh gigitan nyamuk Anopheles. Kabupaten Manokwari yang berada di wilayah Provinsi Papua Barat merupakan daerah endemis tinggi malaria dengan Annual Parasite Incidence (API) 22,88 tahun 2018, angka ini termasuk dalam kategori High Case Incidence (HCI) > 5. Tujuan penelitian ini yaitu menganalisis kasus malaria pada pasien yang berobat di Puskemas Sanggeng dari bulan November sampai Desember 2019 . Berdasarkan jumlah kasus, karakteristik pasien (berdasarkan kelompok umur dan jenis kelamin) dan jenis Plasmodium. Metode penelitian ini adalah deskriptif dengan pendekatan laboratorium yaitu pemeriksaan secara mikroskopik sediaan darah tipis dan sediaan darah tebal menggunakan mikroskop. Hasil penelitian dari 730 pasien terdapat 35 sedian darah positif malaria. Prevalensi usia yang kena malaria paling tinggi pada usia ≥ 15 tahun (51,42 %). Penderita malaria berdasarkan jenis kelamin terbanyak adalah perempuan (51,43 %) dan jenis plasmodium yang ditemukan adalah Plasmodium falciparum (20%) dan Plasmodium vivax (80%). Kata kunci: Plasmodium falciparum, Plasmodium vivax, Anopheles, endemis malaria