nitrocellulose membrane
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2022 ◽  
Vol 26 ◽  
pp. 101305
Author(s):  
Ruihua Tang ◽  
Ming Yue Xie ◽  
Min Li ◽  
Lei Cao ◽  
Shangsheng Feng ◽  
...  

2022 ◽  
Vol 2022 (1) ◽  
pp. pdb.prot103135
Author(s):  
Edward A. Greenfield

A dot blot is widely used to determine the productivity of a given hybridoma. This assay can also be used to screen a fusion or subclone plate for productive hybridoma clones. First, a nitrocellulose membrane is coated with an affinity-purified goat or rabbit anti-mouse immunoglobulin and then incubated with hybridoma tissue culture supernatant. Monoclonal antibodies in the supernatant are then “captured” on the coated nitrocellulose membrane surface and detected by screening with horseradish peroxidase (HRP).


2021 ◽  
Vol 68 (4) ◽  
pp. 781-790
Author(s):  
Burak Şener ◽  
Ömür Baysal ◽  
Said Nadeem ◽  
Ragıp Soner Silme

A rapid and confident tool to identify and diagnose bacterial pathogens with more accuracy using DNA as fingerprints is necessary. Herein, we report a smart chemosensor having a terminal adenine sticking to the thymine of single-stranded DNA (ssDNA) through supramolecular interactions and, which leaves ssDNA when the same ssDNA matches with the targeting desired DNA. We have synthesized a naked-eye coloured chemosensor with carbazole. As a model genetic material, DNA of Clavibacter michiganensis subsp. michiganensis was hybridized to ssDNA and immobilized over nitrocellulose membrane. The prepared adenine-chemosensor, by passing through the nitrocellulose-ssDNA membrane caused the formation of ssDNA nitrocellulose-ssDNA-adenine-chemosensor. FTIR results of the immobilized ssDNAs showed that the matching of same ssDNA releases the adenine-chemosensor from the surface of nitrocellulose-ssDNA that results in formation of the double stranded DNA. The selectivity of chemosensor was also confirmed with different bacterial DNA (Bacillus subtilis) as control. These data highlights accurate and reliable results of a new diagnostic kit prototype promising for further studies, which is able to diagnose DNA quickly and precisely.


2021 ◽  
Author(s):  
Ken Christensen

Transferring your proteins from your SDS-PAGE gel to a nitrocellulose membrane for western blot is quick and straightforward.


2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Daniel M. Kainz ◽  
Bastian J. Breiner ◽  
Susanna M. Früh ◽  
Tobias Hutzenlaub ◽  
Roland Zengerle ◽  
...  

AbstractDespite the widespread application of point-of-care lateral flow tests, the viscosity dependence of these assay results remains a significant challenge. Here, we employ centrifugal microfluidic flow control through the nitrocellulose membrane of the strip to eliminate the viscosity bias. The key feature is the balancing of the sample flow into the cassette of the lateral flow test with the air flow out of the cassette. A viscosity-independent flow rate of 3.01 ± 0.18 µl/min (±6%) is demonstrated for samples with viscosities ranging from 1.1 mPas to 24 mPas, a factor greater than 20. In a model human IgG lateral flow assay, signal-intensity shifts caused by varying the sample viscosity from 1.1 mPas to 2.3 mPas could be reduced by more than 84%.


Author(s):  
Shaobo Du ◽  
Bin Liu ◽  
Zhan Li ◽  
Hongxin Tan ◽  
Wei Qi ◽  
...  

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 134
Author(s):  
Hanie Safarpour ◽  
Hasan Majdi ◽  
Ali Masjedi ◽  
Abdol Sattar Pagheh ◽  
Maria de Lourdes Pereira ◽  
...  

Human echinococcosis is a serious parasitic diseasethat still affects millions of people in many parts of the world. Since it can offer a critical threat to people’s health, it is important to discover a rapid, convenient, and economical method for detection. Herein, we propose a novel point of care assay, namely, an enhanced immuno-dot-blot assay for diagnosis of cystic echinococcosis (hydatidosis). This method is based on the formation of a sandwich complex between a goldnanoprobe (chitosan–gold nanoparticleprotein A) and hydatid cyst antigen (Ag B), which holds anti-Ag B antibodies. Briefly, protein A was conjugated to chitosan–gold nanoparticles via glutaraldehyde chemistry. Then, Ag B was immobilized on the surface of a nitrocellulose membrane, which was followed by the addition of the sera sample and gold nanoprobes. The positive signals were easily detectable by naked eye. The signal intensity of this biosensor was proportional to the concentration of active anti-Echinococcus granulosus antibodies on the surface of the nanoparticles, titer of antibodies in the sera samples, and concentration of Ag B coated on the nitrocellulose membrane. The minimum concentration to use the protein A for conjugation to detect titer of anti-Echinococcus IgGand the concentration of Ag B coated in nitrocellulose membrane were 0.5 and 0.3 mg/mL, respectively. This enhanced immuno-dot-blot assay offers a simple diagnostic technique withoutthe need for expensive equipment for diagnosis of echinococcosis.


2021 ◽  
Vol 100 ◽  
pp. 193-202 ◽  
Author(s):  
Li Wu ◽  
Xinmiao Jin ◽  
Tongqian Zhao ◽  
Haipo Wang ◽  
Zhifeng Dai

RSC Advances ◽  
2021 ◽  
Vol 11 (43) ◽  
pp. 26493-26501
Author(s):  
Xue Wang ◽  
Chao-Hua Xue ◽  
Dong Yang ◽  
Shun-Tian Jia ◽  
Ya-Ru Ding ◽  
...  

We constructed a new type of ICT strip by replacing the conventional nitrocellulose membrane with an electrospin-coated nitrocellulose (ENC) fibrous membrane, and the ICT strip could obtain an HCG detection limit of 0.22 mIU mL−1, and an LH detection limit of 0.36 mIU mL−1.


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