IMPACT OF PLANT GROWTH INHIBITORS ON THE GROWTH AND YIELD OF SORGHUM

Field experiment was carried out during the spring and fall seasons of 2019 at the Abu Ghraib Research Station of the Agricultural Research Office/ Ministry of Agriculture, was aimed to investigate the effect of plant growth inhibitors on growth and yield of several sorghum varieties .Randomized Complete Block Design within split plot arrangement with three replications was used. The main plot consists of three growth inhibitors (Cycocel, Ethiphon, and Mebiquat), which were added at stages six and eight leaf stage, in addition to the control treatment (distilled water only), the sub plot was included three cultivars (Mabrouk, Buhooth 70 and Giza 113). The results showed in both seasons significant interaction between cultivars and growth inhibitors in most of the studied traits. The plants of the variety Giza 113 sprayed with Ethiphon recorded the lowest period of reaching physiological maturity, the highest average dry matter yield, and the highest weight of 1000 grains (89.00 days, 17.32 ton ha-1, and 39.33 gm) respectively. The same variety recorded the highest content of chlorophyll in the leaves and the highest grain yield due to the effect of Mebiquat (49.50 spad, 3.93 ton ha-1. The cultivar Buhooth 70 with the effect of Cycocel achieved the highest average of dry matter yield of 33.27 ton ha-1.

Enhance Growth and Flower Quality of Chrysanthemum indicum L. with Application of Plant Growth Retardants

Ornamental plants are cultivated worldwide. Chrysanthemum is classified as one of the most important cut and potted flowers in most of the countries. The consumer’s expectation is to find small–compact, and full of inflorescences plants. To meet these demands, growers are tending to use plant growth retardants. Three Chrysanthemum indicum L. varieties (‘Smola White’, ‘Arber’ and ‘Vienna White’) were assessed by using four plant growth regulators (PP–Bumper 250 EC; CC–Stabilan SL; MP–Medax Top SC; and PD–Toprex SC). Results indicate that treated plants show significant decrease in the assessed parameters, although in some cases growth could be a variety–dependent factor. It can also be concluded that retardants inhibit chrysanthemum growth. PD treatments greatly inhibited the growth of the plant, and also had a negative effect on inflorescences. In conclusion, the present work strengthens the possibility of using retardants as plant growth inhibitors in Chrysanthemum cultivation.

Impact of Cymbopogon flexuosus (Poaceae) essential oil and primary components on the eclosion and larval development of Aedes aegypti

The current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti. We treated eggs with 6, 18, or 30 mg L−1 and larvae with 3 or 6 mg L−1 of EO and its major compounds (citral and geranyl acetate). Citral and geranyl acetate were evaluated at 18, 30, and 42 mg L−1 and compared with commercial growth inhibitors (diflubenzuron and methoprene). We measured larval head diameter, siphon length, and larval length. Finally, we examined concentrations of molt hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography coupled to mass spectrometry. All geranyl acetate concentrations decreased egg hatching, while EO altered molting among larval instars and between larvae and pupae, with an increase in the larval length (3 mg L−1: 6 ± 0.0 mm; 6 mg L−1: 6 ± 0.7 mm) and head width (3 mg L−1: 0.8 ± 0 mm; 6 mg L−1: 0.8 ± 0.0 mm) compared with the control group. We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, namely decreased hormone concentrations, an extended larval period, and death.

Programming cell growth into different cluster shapes using diffusible signals

Advances in genetic engineering technologies have allowed the construction of artificial genetic circuits, which have been used to generate spatial patterns of differential gene expression. However, the question of how cells can be programmed, and how complex the rules need to be, to achieve a desired tissue morphology has received less attention. Here, we address these questions by developing a mathematical model to study how cells can collectively grow into clusters with different structural morphologies by secreting diffusible signals that can influence cellular growth rates. We formulate how growth regulators can be used to control the formation of cellular protrusions and how the range of achievable structures scales with the number of distinct signals. We show that a single growth inhibitor is insufficient for the formation of multiple protrusions but may be achieved with multiple growth inhibitors, and that other types of signals can regulate the shape of protrusion tips. These examples illustrate how our approach could potentially be used to guide the design of regulatory circuits for achieving a desired target structure.

Diploid genome assembly of Kluyveromyces marxianus NRRL Y-50883 (SLP1)

The yeast Kluyveromyces marxianus SLP1 has the potential for application in biotechnological processes because it can metabolize several sugars and produce high-value metabolites. K. marxianus SLP1 is a thermotolerant yeast isolated from the mezcal process, and it is tolerant to several cell growth inhibitors such as saponins, furan aldehydes, weak acids, and phenolics compounds. The genomic differences between dairy and non-dairy strains related to K. marxianus variability are a focus of research attention, particularly the pathways leading this species toward polyploidy. We report the diploid genome assembly of K. marxianus SLP1 non-lactide strain into 32 contigs to reach a size of ∼12 Mb (N50 = 1.3Mb) and a ∼39% GC content. Genome size is consistent with the k-mer frequency results. Genome annotation by Funannotate estimated 5000 genes in haplotype A and 4910 in haplotype B. The enriched annotated genes by ontology show differences between alleles in biological processes and cellular component. The analysis of variants related to DMKU3 and between haplotypes shows changes in LAC12 and INU1, which we hypothesize can impact carbon source performance. This report presents the first polyploid K. marxianus strain recovered from non-lactic fermenting medium.

Synthesis of 3,5-Bis(trifluoromethyl)phenyl-Substituted Pyrazole Derivatives as Potent Growth Inhibitors of Drug-Resistant Bacteria

Enterococci and methicillin-resistant S. aureus (MRSA) are among the menacing bacterial pathogens. Novel antibiotics are urgently needed to tackle these antibiotic-resistant bacterial infections. This article reports the design, synthesis, and antimicrobial studies of 30 novel pyrazole derivatives. Most of the synthesized compounds are potent growth inhibitors of planktonic Gram-positive bacteria with minimum inhibitory concertation (MIC) values as low as 0.25 µg/mL. Further studies led to the discovery of several lead compounds, which are bactericidal and potent against MRSA persisters. Compounds 11, 28, and 29 are potent against S. aureus biofilms with minimum biofilm eradication concentration (MBEC) values as low as 1 µg/mL.

Curcumin as an Adjuvant to Cancer Immunotherapy

The components of the immune system play a very sincere and crucial role in combating tumors. However, despite their firm efforts of elimination, tumor cells cleverly escape the surveillance process by adopting several immune evasion mechanisms. The conversion of immunogenicity of tumor microenvironment into tolerogenic is considered as a prime reason for tumor immune escape. Therapeutically, different immunotherapies have been adopted to block such immune escaping routes along with better clinical outcomes. Still, the therapies are haunted by several drawbacks. Over time, curcumin has been considered as a potential anti-cancer molecule. Its potentialities have been recorded against the standard hallmarks of cancer such as continuous proliferation, escaping apoptosis, continuous angiogenesis, insensitivity to growth inhibitors, tissue invasion, and metastasis. Hence, the diversity of curcumin functioning has already been established and exploration of its application with immunotherapies might open up a new avenue for scientists and clinicians. In this review, we briefly discuss the tumor’s way of immune escaping, followed by various modern immunotherapies that have been used to encounter the escaping paths and their minute flaws. Finally, the conclusion has been drawn with the application of curcumin as a potential immune-adjuvant, which fearlessly could be used with immunotherapies for best outcomes.