Developing Digital Photomicroscopy

(1) The need for efficient ways of recording and presenting multicolour immunohistochemistry images in a pioneering laboratory developing new techniques motivated a move away from photography to electronic and ultimately digital photomicroscopy. (2) Initially broadcast quality analogue cameras were used in the absence of practical digital cameras. This allowed the development of digital image processing, storage and presentation. (3) As early adopters of digital cameras, their advantages and limitations were recognised in implementation. (4) The adoption of immunofluorescence for multiprobe detection prompted further developments, particularly a critical approach to probe colocalization. (5) Subsequently, whole-slide scanning was implemented, greatly enhancing histology for diagnosis, research and teaching.

Quantitative Identification Cracks of Heritage Rock Based on Digital Image Technology

Digital image processing technologies are used to extract and evaluate the cracks of heritage rock in this paper. Firstly, the image needs to go through a series of image preprocessing operations such as graying, enhancement, filtering and binaryzation to filter out a large part of the noise. Then, in order to achieve the requirements of accurately extracting the crack area, the image is again divided into the crack area and morphological filtering. After evaluation, the obtained fracture area can provide data support for the restoration and protection of heritage rock. In this paper, the cracks of heritage rock are extracted in three different locations.The results show that the three groups of rock fractures have different effects on the rocks, but they all need to be repaired to maintain the appearance of the heritage rock.

Color Enhancement of Low Illumination Garden Landscape Images

The unfavorable shooting environment severely hinders the acquisition of actual landscape information in garden landscape design. Low quality, low illumination garden landscape images (GLIs) can be enhanced through advanced digital image processing. However, the current color enhancement models have poor applicability. When the environment changes, these models are easy to lose image details, and perform with a low robustness. Therefore, this paper tries to enhance the color of low illumination GLIs. Specifically, the color restoration of GLIs was realized based on modified dynamic threshold. After color correction, the low illumination GLI were restored and enhanced by a self-designed convolutional neural network (CNN). In this way, the authors achieved ideal effects of color restoration and clarity enhancement, while solving the difficulty of manual feature design in landscape design renderings. Finally, experiments were carried out to verify the feasibility and effectiveness of the proposed image color enhancement approach.

Discovery of EDA-Complex Photocatalyzed Reactions Using Multidimensional Image Processing: Iminophosphorane Synthesis as a Case Study

Herein, we report a multidimensional screening strategy for the discovery of EDA-complex photocatalyzed reactions using only photographic devices (webcam, cellphone) and TLC analysis. An algorithm was designed to identify automatically EDA-complex reactive mixtures in solution from digital image processing in a 96-wells microplate and by TLC-analysis. The code highlights the region of absorption of the mixture in the visible spectrum, and the quantity of the color change through grayscale values. Furthermore, the code identifies automatically the blurs on the TLC plate and classifies the mixture as colorimetric reactions, non-reactive or potentially reactive EDA mixtures. This strategy allowed us to discover and then optimize a new EDA-mediated approach for obtaining iminophosphoranes in up to 90% yield.