Investigation of soil nutrients and associated rhizobacterial communities in different sugarcane genotypes in relation to sugar content

Background Plant microbiomes and soil are bridged by rhizobacteria, maintaining and improving plant health and growth in different aspects. This study was conducted in the field station of the Guangxi University, Fusui, China. We investigated soil nutrients, root morphology and rhizosphere bacterial composition, and community structures in 18 sugarcane genotypes concerning sugar content under the same environmental condition. Results Most of the rhizosphere microbiomes of these genotypes exhibited similar bacterial compositions. However, the evaluated genotypes harbored a significant effect and difference in the abundance of operational taxonomic units and bacterial composition in the rhizosphere compartments. Alpha diversity analysis on the rhizosphere microbiome showed a significant difference in the bacterial diversity (Shannon index, p < 0.001) and OTU richness (Chao1, p < 0.001). The principal coordinate analysis (PCoA) and hierarchical cluster analysis revealed that the genotype replicated samples grouped, indicating their similarity. Besides, these genotypes also differed significantly in terms of root structure and soil properties. A significant genotypic effect (p < 0.05) was found in the root traits except for rooting depth. The soil chemical properties were significantly different among the evaluated genotypes. Furthermore, sucrose content was strongly correlated with the total root length (TRL) and rooting depth. Genotypes (FN-1702, GUC-3, ZZ-13, ZZ-10, ZZ-6) were the best performing and distinct in bacterial diversity, root structure, soil parameters and sucrose content. Conclusion The results showed a closely related and highly conserved bacterial community of the rhizosphere microbiome. The rhizosphere microbiome diversity and related bacterial communities were highly associated with the relevant plant taxa, probably at the order level. As a result, it is possible to conclude that the host genotype and the same environmental condition influenced the rhizosphere microbiome via root phenes. Future research regarding plant phenes and microbiome functional groups could be considered an essential factor. Graphic abstract

Marker-trait association study for root-related traits in chickpea (Cicer arietinum L.)

<p class="042abstractstekst">Root structure modification can improve important agronomic traits including yield, drought tolerance and nutrient deficiency resistance. The aim of the present study was to investigate the diversity of root traits and to find simple sequence repeat (SSR) markers linked to root traits in chickpea (<em>Cicer arietinum </em>L.). This research was performed using 39 diverse accessions of chickpea. The results showed that there is significant variation in root traits among chickpea genotypes. A total of 26 alleles were detected 26 polymorphic bands were produced by 10 SSR markers in the eight linkage groups (LG). The results indicated that there is substantial variability present in chickpea<strong> </strong>germplasm for root traits.<strong> </strong>By analyzing the population structure, four subpopulations were identified.<strong> </strong>PsAS2, AF016458, 16549 and 19075 SSR markers on LG1, LG3, LG2 and LG1 linkage group respectively were<strong> </strong>associated with root traits<strong>.</strong> The research findings provide valuable information for improving root traits for chickpea breeders.</p>

Marker-trait association study for root-related traits in chickpea (Cicer arietinum L.)

<p class="042abstractstekst">Root structure modification can improve important agronomic traits including yield, drought tolerance and nutrient deficiency resistance. The aim of the present study was to investigate the diversity of root traits and to find simple sequence repeat (SSR) markers linked to root traits in chickpea (<em>Cicer arietinum </em>L.). This research was performed using 39 diverse accessions of chickpea. The results showed that there is significant variation in root traits among chickpea genotypes. A total of 26 alleles were detected 26 polymorphic bands were produced by 10 SSR markers in the eight linkage groups (LG). The results indicated that there is substantial variability present in chickpea<strong> </strong>germplasm for root traits.<strong> </strong>By analyzing the population structure, four subpopulations were identified.<strong> </strong>PsAS2, AF016458, 16549 and 19075 SSR markers on LG1, LG3, LG2 and LG1 linkage group respectively were<strong> </strong>associated with root traits<strong>.</strong> The research findings provide valuable information for improving root traits for chickpea breeders.</p>

Methods of In Situ Quantitative Root Biology

When dealing with plant roots, a multiscale description of the functional root structure is needed. Since the beginning of 21st century, new devices such as laser confocal microscopes have been accessible for coarse root structure measurements, including three-dimensional (3D) reconstruction. Most researchers are familiar with using simple 2D geometry visualization that does not allow quantitative determination of key morphological features from an organ-like perspective. We provide here a detailed description of the quantitative methods available for 3D analysis of root features at single-cell resolution, including root asymmetry, lateral root analysis, cell size and nuclear organization, cell-cycle kinetics, and chromatin structure analysis. Quantitative maps of the root apical meristem (RAM) are shown for different species, including Arabidopsis thaliana (L.), Heynh, Nicotiana tabacum L., Medicago sativa L., and Setaria italica (L.) P. Beauv. The 3D analysis of the RAM in these species showed divergence in chromatin organization and cell volume distribution that might be used to study root zonation for each root tissue. Detailed protocols and possible pitfalls in the usage of the marker lines are discussed. Therefore, researchers who need to improve their quantitative root biology portfolio can use them as a reference.

Management of Severely Aberrant Permanent First Molars in Molar Root–Incisor Malformation Patients: Case Series and a Guideline

Recently, a new type of dental anomaly, a molar–incisor malformation or molar root–incisor malformation (MRIM), was recognized. The disease phenotype is now relatively well characterized; however, its etiology and disease-mechanism need to be elucidated. The affected teeth do not respond well to conventional treatment because of severe malformation and an unusual root structure. In this study, we present the treatment of MRIM cases with the extraction of severely aberrant permanent first molars (PFMs) and suggest that the PFM extractions are performed when it is clear that third molars will develop. The purpose of this report was primarily to present amendments to the guidelines for the treatment of patients with MRIM.

Interaction of Kreyellidae sp. and plant growth promoting bacteria influences the soil characteristics and root structure of rice plants

Plant growth and productivity depend on the complex and dynamic interaction between the plant roots and soil microflora. At present, the research on rhizosphere associated microbes’ is largely focused on bacteria and fungi; whereas the interaction of soil protists with plants and other microbes remain unexplored. The present study aimed to investigate the impact of a ciliate (Kreyellidae sp. C5) and two plant growth-promoting bacteria (PGPB) i.e., Pseudomonas sp. (Ps) and Enterobacter sp. (Ec), on the growth of rice plants. It was observed that the protist-PGPB interaction significantly modified the root structure leading to an enhanced outgrowth of lateral roots (272.08% − 380.41%) and seminal roots (190.40% -250.45%), in addition to an increase in the primary root length (Turkey’s HSD, p < 0.05). The Phospholipid Fatty Acid (PLFA) analysis indicated a striking shift in the overall soil microbial communities due to the presence of a predator. The combined treatments (with C5Ps and C5Ec) further increased the Microbial Carbon Biomass (MBC) to 223.59% and 310.57% as compared to control and PGPB treatments respectively. A similar enhancement of dehydrogenase enzyme activity was observed in soil samples of rice plants on combined treatments. In contrast, the alkaline phosphatase and fluorescein diacetate enzyme activities were recorded to be more in soil samples treated with PGPB. The combined treatment of rice plants also enhanced the uptake of N and P moderately, as compared to PGPB treated plants. However, this enhancement was significant compared to control plants. The colony-forming unit (CFU) and most probable number (MPN) was found to be more in C5Ec (131.0 ± 3.70×1011 and 5.12 ± 0.06) and C5Ps (24.10 ± 2.19×1010 and 10.52 ± 0.39), as compared to control and PGPB treated soil samples. In conclusion, this is the first study that demonstrates significant modification of root structure and increased nutrient uptake by rice plants through interaction between Kreyellidae sp. and PGPB. In addition, we also report improved respiration and diverse microbial population in soil samples on combined treatment of rice plants.

Pathways of initial consonant loss

This paper investigates the historical loss of root-initial consonants, using a case study of Middle Paman languages of Cape York Peninsula, in northeastern Australia. Systematic loss of initial consonants is a typologically unusual phenomenon, mainly found in Australia, that has often been regarded as a starting point for far-reaching changes in root structure, phonotactics and even phoneme inventory. So far, the literature has focused mainly on identifying phonetic causes of initial loss. This study focuses on the actual processes and pathways of initial loss, which is an equally important part of the historical puzzle. Specifically, it shows that there are multiple pathways for initial loss: it can be the result of a gradual phonetic process involving intermediate steps like lenition, as is assumed in part of the literature, but it can also be due to more abrupt processes involving borrowing and even morphosyntactic alternations. This adds to a more diversified model of how initial loss actually proceeds, which together with earlier work on the diversity of phonetic causes of initial loss produces a more comprehensive understanding of this typologically and diachronically unusual phenomenon.