Wild mushroom poisoning in Kumaon region of Uttarakhand: a case series

Mushrooms have been dietary source in hilly and ethnic tribes of India. More than 5000 mushroom species are known worldwide and nearly 100 species are known to be poisonous for humans. Mushroom poisoning occurs due to unintentional and accidental ingestion of poisonous mushroom due to misidentification of poisonous variety as edible one. There has been increasingly incidence of reporting of mushroom poisoning cases nowadays. Here we are reporting case series of 4 patients admitted hailing from same village with accidental ingestion of poisonous mushrooms with clinical-laboratory profile and outcome at our institution. Mushroom Poisoning is an emerging healthcare concern nowadays. Education and mass awareness for identification of poisonous mushrooms is an important preventive measure. Early hospitalization, proper hydration, gastric decontamination, silibinin and N- acetyl cysteine therapy with hepato-renal support constitutes mainstay of treatment. Delay in treatment and complications results in poor prognosis and mortality.

Two new Inosperma (Inocybaceae) species with unexpected muscarine contents from tropical China

An accurate identification of poisonous mushrooms and the confirmation of the toxins involved are both of great importance in the treatment of mushroom poisoning incidents. In recent years, cases of mushroom poisoning by Inosperma spp. have been repeatedly reported from tropical Asia. It is urgent to know the real species diversity of Inosperma in this region. In the present study, we proposed two new Inosperma species from tropical Asia, namely I. muscarium and I. hainanense. They were described based on morphology and multilocus phylogeny. Detailed descriptions, color photographs and the discussion with other closely related species of the two new taxa were provided. In addition, a comprehensive muscarine determination of these two new species using ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) approach has been performed. Results showed that these two species were muscarine positive, with a content of 16.03 ± 1.23 g/kg in I. muscarium and a content of 11.87 ± 3.02 g/kg in I. hainanense, much higher than the known species I. virosum. Recovery of muscarine ranged from 93.45% to 97.25%, and the average recovery is 95.56%.

“The-World-Around-Us” Lesson in the Fourth Grade: “Poisonous Animals. Poisonous Mushrooms”

The article discusses the structure and methodology of conducting a lesson with fourth-graders on the natural history topic “Poisonous animals. Poisonous Mushrooms “within the framework of mastering the content of the training course “The-World-Around-Us”. The teacher implements the set goals of the lesson on the basis of the use of problem situations, comparison of drawings with the corresponding texts, the widespread use of visual material and works of art. Simultaneously with the expansion of knowledge about poisonous animals and mushrooms, younger schoolchildren develop ideas about the

Pengenalan Jamur Yang Dapat Dikonsumsi Menggunakan Metode Transfer Learning Pada Convolutional Neural Network

Not all mushrooms are edible because some are poisonous. The edible or poisonous mushrooms can be identified by paying attention to the morphological characteristics of mushrooms, such as shape, color, and texture. There is an issue: some poisonous mushrooms have morphological features that are very similar to edible mushrooms. It can lead to the misidentification of mushrooms. This work aims to recognize edible or poisonous mushrooms using a Deep Learning approach, typically Convolutional Neural Networks. Because the training process will take a long time, Transfer Learning was applied to accelerate the learning process. Transfer learning uses an existing model as a base model in our neural network by transferring information from the related domain. There are Four base models are used, namely MobileNets, MobileNetV2, ResNet50, and VGG19. Each base model will be subjected to several experimental scenarios, such as setting the different learning rate values for pre-training and fine-tuning. The results show that the Convolutional Neural Network with transfer learning method can recognize edible or poisonous mushrooms with more than 86% accuracy. Moreover, the best accuracy result is 92.19% obtained from the base model of MobileNetsV2 with a learning rate of 0,00001 at the pre-training stage and 0,0001 at the fine-tuning stage.

What’s for dinner this time?: DNA authentication of “wild mushrooms” in food products sold in the USA

Mushrooms have been consumed by humans for thousands of years, and while some have gastronomic and nutritional value, it has long been recognized that only select species of mushrooms are suitable for consumption. Adverse health effects of consuming poisonous mushrooms range from mild illness to death. Many valuable edible mushrooms are either impractical or unable to be grown commercially, requiring them to be harvested from the wild. In the U.S., products containing these wild-collected mushrooms are often sold with the nonspecific and undefined label “wild mushrooms,” although in some cases particular species are listed in the ingredients. However, the ambiguity of the definition of “wild mushrooms” in foods makes it impossible to know which species are involved or whether they are truly wild-collected or cultivated varieties. As a consequence, any individual adverse reactions to consuming the mushrooms in these products cannot be traced to the source due to the minimal regulations around the harvest and sale of wild mushrooms. For this study, we set out to shed light on what species of fungi are being sold as “wild mushrooms” using DNA metabarcoding to identify fungal contents of various food products acquired from locally sourced grocers and a large online retail site. Twenty-eight species of mushroom were identified across 16 food products, ranging from commonly cultivated species to wild species not represented in global DNA databases. Our results demonstrate that “wild mushroom” ingredients often consist entirely or in part of cultivated species such as the ubiquitous white and brown “button” mushrooms and portabella (Agaricus bisporus), oyster (Pleurotus spp.) and shiitake (Lentinula edodes). In other cases truly wild mushrooms were detected but they were not always consistent with the species on the label. More alarmingly, a few products with large distribution potential contained species whose edibility is at best dubious, and at worst potentially toxic.

Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach

Most species in the genus Amanita are ectomycorrhizal fungi comprising both edible and poisonous mushrooms. Some species produce potent cyclic peptide toxins, such as α-amanitin, which places them among the deadliest organisms known to mankind. These toxins and related cyclic peptides are encoded by genes of the “MSDIN” family (named after the first five amino acid residues of the precursor peptides), and it is largely unknown to what extent these genes are expressed in the basidiocarps. In the present study, Amanita rimosa and Amanita exitialis were sequenced through the PacBio and Illumina techniques. Together with our two previously sequenced genomes, Amanita subjunquillea and Amanita pallidorosea, in total, 46 previously unknown MSDIN genes were discovered. The expression of over 80% of the MSDIN genes was demonstrated in A. subjunquillea. Through a combination of genomics and mass spectrometry, 12 MSDIN genes were shown to produce novel cyclic peptides. To further confirm the results, three of the cyclic peptides were chemically synthesized. The tandem mass spectrometry (MS/MS) spectra of the natural and the synthetic peptides shared a majority of the fragment ions, demonstrating an identical structure between each peptide pair. Collectively, the results suggested that the genome-guided approach is reliable for identifying novel cyclic peptides in Amanita species and that there is a large peptide reservoir in these mushrooms.

Poisoning Case of Chlorophyllum cf. molybdites in Indonesia

Wild edible mushrooms are one the germplasms which consumed by some people in Indonesia. However, due to the similar morpholgical appearance from one mushroom to another, cases of wild mushroom poisoning might be occurred as the result of the misidentification at foraging time. One of the common poisonous mushrooms which easily found around residence area is Chlorophyllum molybdites. To date, there is no database of mushroom poisoning cases in Indonesia. The aims of this research was to provide the information regarding C. molybdites poisoning cases in Indonesia. This is a literature-based quantitative research. During the last 10 years, it is suspected that there have been 4 cases of Chlorophyllum cf. molybdites poisoning in Indonesia with a total of 34 victims and none has died. This mushroom is known to contain toxic compounds, namely polymeric protein. The main constraint related to determining the taxonomic identity of poisonous mushroom, is the lack of information and documentation of samples that cause poisoning in Indonesia. This research explained how to recognize the character of C. molybdites, information on its distribution, and its toxicity aspects. With the complete information as a medium for disseminating mycology knowledge, it is hoped that cases of wild mushroom poisoning can be prevented in the future in Indonesia.

Chlorophyllum Molybdites “Delicious Poisoning Snack” in Patients Diagnosed with Acute Gastroenteritis

Introduction: Since time immemorial, mushrooms have been used as a part of human diet, some of them are very well known for their nutritive and medicinal properties and some are known to cause poisoning to the human body. A number of post ingestion fatalities due to poisonous mushrooms has been reported worldwide. These poisonous mushrooms are often misidentified as edible ones, which accounts for accidental poisoning.Objective: The main objective of this report was to describe the clinical manifestations of mushroom poisoning cases presented at the Emergency Department (ED), Taiping Hospital.Case Presentation: There were two cases presented, who suffered from moderate dehydration due to acute gastroenteritis after taking 'delicious mushrooms', also known as Chlorophyllum Molybdites. This study found that both cases had complaints of abdominal cramping, diarrhoea and vomiting more than twenty times a day. There was no history of numbness or weakness noted, and no chest pain or shortness of breath. On arrival, both cases presented signs of moderate dehydration with coated tongue and normal blood pressure, with slightly increased in temperature (37.30C). Abdomen was soft but discomfort upon palpation and described as bloated. Both cases were resuscitated with 20ml/kg normal saline. Charcoal, antiemetic, proton pump inhibitor and ceftriaxone antibiotic were given at the ED. Both survived and were treated as infectious acute gastroenteritis. Nausea and vomiting were the most common early symptoms of intoxication and should be considered as a medical emergency. Alpha Amanitin levels should be checked where possible if amanita poisoning is suspected. An early diagnosis and immediate treatment are required for a successful outcome.Conclusion: All patients with the history of mushroom ingestion should be admitted. If laboratory detection of toxin is not available, history of mushroom ingestion, clinical manifestation and their trends could define mushroom poisoning.International Journal of Human and Health Sciences Supplementary Issue: 2021 Page: S17

Chemistry and Toxicology of Major Bioactive Substances in Inocybe Mushrooms

Mushroom poisoning has always been a threat to human health. There are a large number of reports about ingestion of poisonous mushrooms every year around the world. It attracts the attention of researchers, especially in the aspects of toxin composition, toxic mechanism and toxin application in poisonous mushroom. Inocybe is a large genus of mushrooms and contains toxic substances including muscarine, psilocybin, psilocin, aeruginascin, lectins and baeocystin. In order to prevent and remedy mushroom poisoning, it is significant to clarify the toxic effects and mechanisms of these bioactive substances. In this review article, we summarize the chemistry, most known toxic effects and mechanisms of major toxic substances in Inocybe mushrooms, especially muscarine, psilocybin and psilocin. Their available toxicity data (different species, different administration routes) published formerly are also summarized. In addition, the treatment and medical application of these toxic substances in Inocybe mushrooms are also discussed. We hope that this review will help understanding of the chemistry and toxicology of Inocybe mushrooms as well as the potential clinical application of its bioactive substances to benefit human beings.