Use of glass waste in the production of metakaolin-based geopolymer submitted to room temperature and thermal curing

Metakaolinis the principal raw material utilized in the synthesis of geopolymers, although its ratio of silica and alumina contents is not ideal. Normally, the SiO2 content is adjusted with the use of silicates present in the activating solution. An eco-efficient alternative would be the use of glass waste as an additional source of silica.This work evaluates the efficiency of the alkaline activation of metakaolin, using potassium hydroxide and silicate, with and without the substitution of 12.5% of metakaolin by microparticles of glass. The efficiency of the alkaline activation was evaluated by X ray diffractometry, spectroscopy in the infrared region with the Fourier transform, nuclear magnetic resonance spectroscopy of 27Al and 29Si, specific mass and compressive strength. The results indicate the occurrence of geopolymerization with and without the use of glass waste. It was observed that the substitution of 12.5% favors the mechanical performance of the compounds at 28 days, with increases by 37% and 47% in the mechanical strength of the material with thermal curing and ambient temperature curing, respectively.

Current and Future Methodology for Quantitation and Site-specific Mapping the Location of DNA Adducts

Formation of DNA adducts is a key event for a genotoxic mode of action and its formation is often use as surrogate for mutation and cancer. Interest in DNA adducts are twofold, first, to demonstrate exposure, and second, to link DNA adduct location to subsequent mutations or altered gene regulation. High chemically specific mass spectrometry methods have been established for DNA adduct quantitation and elegant bio-analytic methods utilizing enzymes, various chemistries, and molecular biology methods to visualize the location of DNA adducts. Traditionally, these highly specific methods cannot be combined, and the results are incomparable. Initially developed for single-molecule DNA sequencing, nanopore-type technologies are expected to enable simultaneous quantitation and location of DNA adducts across the genome. We will briefly summarize the current methodologies for state-of-the-art quantitation of DNA adduct levels and mapping of DNA adducts and describe novel single-molecule DNA sequencing technology that is expected to achieve both measures simultaneously. Emerging technologies are expected to soon provide a comprehensive picture of the exposome and identify gene regions susceptible to DNA adduct formation.

Effect of Environmental Factors on Plectranthus Neochilus Volatile Composition: A GC-MS-Based Metabolomics Approach

Plectranthus neochilus Schltr. is an aromatic species, commonly used for digestive, antispasmodic, and analgesic purposes. Although many studies have reported the chemical composition of its essential oil, variations in the volatile profile were observed, which may be due to multiple factors linked to growth and field conditions. In order to detect metabolic variations in this species, we employed a GC-MS-based untargeted metabolomics approach analyzing samples of four P. neochilus individuals collected over a year. From all analyses, 24 mass features were detected and 21 were identified according to their respective chromatographic peaks. All features varied among samples, particularly (2E)-hexenal, 3-octanone and δ-3-carene, which showed the highest coefficient of variation percentage in our study. Although the four individuals presented the same peaks in the chromatograms, significant differences in the intensity of specific mass features were detected between individuals throughout the year. Time of sampling did not affect P. neochilus volatile composition; the chemical profile remained constant throughout the day. Seasonal trends were observed for the species. Winter months coincided with a drop in the intensity of most components. Air temperature showed a positive correlation with some feature intensities, while myrcene and α-thujene resulted in a positive and a negative correlation with rainfall, respectively. This study was the first attempt to correlate metabolic variation and environmental factors in P. neochilus. Our approach was successful in identifying the composition and variation of the headspace volatiles of P. neochilus leaves.

Reconstruction of the Historical (1750–2020) Mass Balance of Bordu, Kara-Batkak and Sary-Tor Glaciers in the Inner Tien Shan, Kyrgyzstan

The mean specific mass balance of a glacier represents the direct link between a glacier and the local climate. Hence, it is intensively monitored throughout the world. In the Kyrgyz Tien Shan, glaciers are of crucial importance with regard to water supply for the surrounding areas. It is therefore essential to know how these glaciers behave due to climate change and how they will evolve in the future. In the Soviet era, multiple glaciological monitoring programs were initiated but these were abandoned in the nineties. Recently, they have been re-established on several glaciers. In this study, a reconstruction of the mean specific mass balance of Bordu, Kara-Batkak and Sary-Tor glaciers is obtained using a surface energy mass balance model. The model is driven by temperature and precipitation data acquired by combining multiple datasets from meteorological stations in the vicinity of the glaciers and tree rings in the Kyrgyz Tien Shan between 1750 and 2020. Multi-annual mass balance measurements integrated over elevation bands of 100 m between 2013 and 2020 are used for calibration. A comparison with WGMS data for the second half of the 20th century is performed for Kara-Batkak glacier. The cumulative mass balances are also compared with geodetic mass balances reconstructed for different time periods. Generally, we find a close agreement, indicating a high confidence in the created mass balance series. The last 20 years show a negative mean specific mass balance except for 2008–2009 when a slightly positive mass balance was found. This indicates that the glaciers are currently in imbalance with the present climatic conditions in the area. For the reconstruction back to 1750, this study specifically highlights that it is essential to adapt the glacier geometry since the end of the Little Ice Age in order to not over- or underestimate the mean specific mass balance. The datasets created can be used to get a better insight into how climate change affects glaciers in the Inner Tien Shan and to model the future evolution of these glaciers as well as other glaciers in the region.

Investigation of Horizontal Wells with Multi-Stage Hydraulic Fracturing Technological Efficiency in the Development of Low-Permeability Oil Reservoirs

The deterioration of the reservoir properties of potential oil and gas bearing areas on mature and green fields, as well as the increase in the volume of hard-to-recover reserves on low-permeable reservoirs set us new challenges in searching and using effective development technologies to maintain and even increase the oil production levels. Based on successful international experience, Russian oil and gas companies use horizontal wells (HW) with multi-stage hydraulic fracturing (MSHF) for the cost-effective development of low-permeable reservoirs. Thus, since the first pilot works of drilling technologies and completion of HW with MSHF in 2011, at the beginning of 2020, over 1,200 HW with MSHF were drilled and came on stream at the fields of LLC RN-Yuganskneftegaz, about half of which are at the exploitation play AS10-12 of the northern license territory (NLT) of the Priobskoye field. In searching the best technologies and engineering solutions, the company tested different lengths of horizontal section of HW, the number of hydraulic fracturing (HF) stages and distances between hydraulic fracturing ports, as well as different specific mass of the proppant per frac port. Recently, there has been a tendency in design solutions to increase the length of the HWs and the number of hydraulic fractures with a decreasing distance between the frac ports and a decreasing specific mass of the proppant per frac port. This work studies the actual and theoretical efficiency of HW with MSHF of various designs (different lengths of horizontal section of HW and the number of HF stages) and to assess the viability of increasing the technological complexity, as well as to analyze the actual impact of loading the proppant mass per port on performing HW with MSHF. The study is based on the results of the analysis of the factual experience accumulated over the entire history of the development of the exploitation play AS10-12 of the NLT of the Priobskoye field of the Rosneft Company. In studying the viability of increasing the technological complexity, especially, increasing the length of horizontal section of HW, increasing the number of HF stages, and reducing the distance between the frac ports: we discovered the typical methodological errors made in analyzing the efficiency of wells of various designs; we developed the methodology for analysis of the actual multiplicity of indicators of wells of various designs, in particular, HW with MSHF relative to deviated wells (DW) with HF; we carried out the statistical analysis of the actual values of the multiplicity of performance indicators and completion parameters of HW with MSHF of various designs relative to the surrounding DW with HF of the exploitation play AS10-12 of the NLT of the Priobskoye field; we performed the theoretical calculation of the multiplicity of the productivity coefficient for the HW with MSHF of various designs relative to DW with HF for the standard development system of the exploitation play AS10-12 of the NLT of the Priobskoye field; we compared the actual and theoretical results. The paper also presents the results of studying the actual effect of changes of proppant's mass per port on performance indicators of HW with MSHF of the same design and with an increase in the number of fractures of the hydraulic fracturing without changing the length of horizontal section of HW. As for performance indicators, being the basis for estimating the efficiency of HW with MSHF of various designs, we used the productivity index per meter of the effective reservoir thickness and the cumulative fluid production per meter of the effective reservoir thickness per a certain period of operation. And as the completion parameters, we used the length of the horizontal section of HW, the number of HF stages, the distance between the frac ports, and the specific mass of the proppant per meter of the effective reservoir thickness per frac port. The results of this work are the determining vector of development for future design decisions in improving the efficiency of HW with MSHF.

The cardiac molecular setting of metabolic syndrome in pigs reveals disease susceptibility and suggests mechanisms that exacerbate COVID-19 outcomes in patients

Although metabolic syndrome (MetS) is linked to an elevated risk of cardiovascular disease (CVD), the cardiac-specific risk mechanism is unknown. Obesity, hypertension, and diabetes (all MetS components) are the most common form of CVD and represent risk factors for worse COVID-19 outcomes compared to their non MetS peers. Here, we use obese Yorkshire pigs as a highly relevant animal model of human MetS, where pigs develop the hallmarks of human MetS and reproducibly mimics the myocardial pathophysiology in patients. Myocardium-specific mass spectroscopy-derived metabolomics, proteomics, and transcriptomics enabled the identity and quality of proteins and metabolites to be investigated in the myocardium to greater depth. Myocardium-specific deregulation of pro-inflammatory markers, propensity for arterial thrombosis, and platelet aggregation was revealed by computational analysis of differentially enriched pathways between MetS and control animals. While key components of the complement pathway and the immune response to viruses are under expressed, key N6-methyladenosin RNA methylation enzymes are largely overexpressed in MetS. Blood tests do not capture the entirety of metabolic changes that the myocardium undergoes, making this analysis of greater value than blood component analysis alone. Our findings create data associations to further characterize the MetS myocardium and disease vulnerability, emphasize the need for a multimodal therapeutic approach, and suggests a mechanism for observed worse outcomes in MetS patients with COVID-19 comorbidity.

Determination of the Specific Mass Rate of Multicomponent Petroleum Products Burn-up

The specific mass burn-up rate of combustible substances (materials) in case of a fire, determines the heat release intensity, the temperature of burning, the intensity of fire development and other parameters. Like the rate of flame propagation, the mass burn-up rate depends on the physical and chemical properties of substances, their aggregate state, as well as other factors. The mass burn-up rate is used in modeling the process of fire development, assessment of the rate of heat release and the intensity of the supply of extinguishing agents to fire extinguishing installations. Currently, the values of the specific mass burn-up rate are given in various reference materials for a limited number of petroleum products. For single-component substances, the desired value can also be determined by calculation. The existing calculation formulas in theory are applicable for both simple and complex substances, and, in this case, there is a need to calculate the values of the specific heat of combustion and evaporation, the specific heat capacity of the substance. However, the process of complex hydrocarbon fuels burn-up differs significantly- it is due to the gradual burning out of individual fractions in their composition. Therefore, for complex substances the calculation should be made considering the changes in density and temperature during the process of burning. The methods for determining the specific mass rate of burnout of multicomponent petroleum products are considered, the universal nomogram and calculation formula are proposed that will allow determining the specific mass rate of burn-up, knowing the density of the petroleum product under normal conditions and its boiling point.

Modeling and simulation of the dynamic behavior of the macaw palm fruit-rachilla system

The macaw palm ( Acrocomia aculeata) is a palm tree native to tropical forests that stand out due to its great potential for oil production. This study was developed with the objective of constructing a high-fidelity model of the macaw palm fruit-rachilla system for the purpose of simulating its dynamic behavior when subjected to mechanical vibrations. The finite element method was used to determine the natural frequencies and modes of vibration of the system. The three-dimensional models of the fruit-rachilla systems were elaborated using CAD3D Fusion 360 software. The modal properties of the fruit-rachilla systems were obtained based on the models developed by varying the elasticity modulus values of the system. The parameters of greatest influence in the estimation of natural frequencies are the elasticity modulus, especially that of the fruit-rachilla joint, and the specific mass. The models that take into account the three-dimensional strains along the rachilla are the least sensitive to variations in the mechanical properties (elasticity modulus and specific mass) and are shown to be more representative of the actual physical system.

Bioethanol Production via Herbaceous and Agricultural Biomass Gasification Integrated with Syngas Fermentation

In this paper, a simulation model based on the non-stoichiometric equilibrium method via ASPEN Plus was established to analyze the gasification performance of 20 herbaceous and agricultural biomasses (H&ABs) linked with syngas fermentation and product purification units for ethanol production. The established simulation model does not consider the gasification system as a black box; it focuses the important processes in gasification such as drying, pyrolysis, gasification, and connection with bioethanol production plants. The results for the 20 H&AB options suggest that the specific mass flow rate of bioethanol from 1 kg of biomass input to the unit is in the range of 99–250 g/kg, and between them, the system fed by hazelnut shell biomass remarkably outranked other alternatives by 241 g/kg production due to the high beneficial results gained from the performance analysis. Additionally, a sensitivity analysis was performed by changing operating conditions such as gasification temperature and air-to-fuel ratio. The modeling results are given and discussed. The established model could be a useful approach to evaluate the impacts of a huge numbers of biomasses and operating parameters on bioethanol output.