The Performance and Carcass Characteristics of Black Baladi Kids Fed Diets with Graded Quantities of Sweet Lupin Grain (Lupinus angustifolius)

A study was conducted to examine how lupin grains (LUPs) feeding affected nutritional intake, digestibility, growth, and carcass characteristics in kids. A total of 24 growing black kids (initial body weight (BW) = 16.4 ± 0.49 kg) were allocated to one of three diets at random. Lupin was included in the diet at a rate of 0 (control; CON), 100 (LUP100), or 200 g/kg (LUP200) of total dry matter (DM). The trial lasted for 91 days divided into 7 and 84 days to be used for adaptation and data collection, respectively. Feed intake was evaluated daily throughout the study. At the commencement and the end of the study, each kid’s body weight was measured to determine its average daily gain (ADG). On day 70, 5 kids were chosen at random from each group to investigate nutrient in vivo digestibility and N balance. At the end of the study, all of the kids were slaughtered to examine carcass features. Nutrient intakes (neutral detergent fiber, acid detergent fiber, and ether extract) were higher (p ≤ 0.01) in LUP-containing diets than in the CON diet. The average daily gain was greater (p ≤ 0.03) in diets containing lupin grains than in the CON diet. Cost of gain ($US/kg growth) was lower (p = 0.004) in kids fed diets containing lupin than the CON diet. Dry matter and CP digestibility rates were greater (p ≤ 0.03) in lupin diets. Retained N was higher (p = 0.04) in lupin-containing diets than in the CON diet. Cold carcass weight was higher (p < 0.05) for kids consuming the LUP100 diet than the CON diet. In lupin diets, carcass cut weights were higher (p < 0.05). Results of the current study indicate that feeding black kids diets containing lupin grains at 100 or 200 g/kg DM basis is cost effective and would increase profitability.

COMPARISON OF GRAIN FEED QUALITY OF DIFFERENT BROAD BEAN GENOTYPES

The objective of the present study was to determine grain feed quality of different broad bean cultivars and lines. For this purpose, Eleven different cultivars and five lines (Emiralem, Histal, Sorgun, Kitik 2003, Sakiz, Eresen 87, Sevil, Reina Mora, Filiz 99, Salkim, Luz De, Otonoto, Seher, EU 4446, Canakkale, Antalya, EU319) were used as the plant material. Experiments were conducted in randomized blocks design with three replications. Effects of genotypes on grain feed quality were found to be highly significant (P≤0.01) Current findings revealed that crude oil content between 0.62 - 1.58%, crude ash content between 1.89 - 3.30%, crude protein content varied between 22.30 - 32.14%, acid detergent fiber (ADF) content between 7.45 - 14.94% and neutral detergent fiber (NDF) content between 16.08 - 28.05%. Seher, EU 4446, Canakkale, Antalya, Filiz 99 and Histal were found to be prominent for hight crude protein and crude oil, thus these genotypes were recommended for animal feeding.

Comparative Study of Local and Imported Enzymes in Improving the Nutritional Values of Agricultural Waste on Performance and Economic Efficiency of Iraqi Lambs

In the current study, we use 25 local Iraqi lambs. With initial body weight (24) kg. The lambs were randomly divided into five treated groups, the first group without treatment as control, the second and third treated with 20%and 40% Agricultural Waste treated with trade enzymes, fourth and fifth treatment 20%and 40% Agricultural Waste treated with local enzymes. The result demonstrated a significant (P<0.01) increase in the quantity of roughages, total feed intake, Neutral Detergent Fiber (NDF) and Acid Detergent Fiber( ADF) and Lignin (ADL) in T5 followed by T4 compared with others treated groups and showed a significant (P<0.01) increase in the digestibility of NDF ,ADF in T4, T5 compare with other treated groups. While T5and T4 recorded Superiority increase in weight gain, and significant improvement in economic cost efficiency with ratio improvement of 5.90, 5.97 % respectively

Different Aspergillus niger Strains and Inoculum Levels Affect the Nutritional Composition of Olive Leaves in Solid-state Fermentation

This study was conducted to investigate the effects of Aspergillus niger strain and inoculum level on the nutritional composition of olive leaves. The experiment had a 2×3 factorial arrangement of treatments with two A. niger strains (ATCC 200345 [A] and ATCC 9142 [B]) and three inoculum levels (104, 106, and 108). Olive leaves were milled to 2 mm and fermented in solid-state by two different A. niger strains and analysed for nutritional composition. Crude protein (CP) was increased linearly as the inoculum level increased. Ether extract (EE) and crude fiber (CF) were decreased linearly in A strain or quadratically in B strain with increased inoculum levels. Crude ash and nitrogen-free extract content did not differ among groups. Strain A increased neutral detergent fiber (NDF) at higher inoculum level, whereas NDF was decreased as inoculum level increased in B strain. The lowest acid detergent fiber (ADF) was obtained from the B strain or 106 inoculum level. The results showed that the effect of two strains on CP and ADF changed similarly with increased inoculum levels. However, two strains affected EE, CF, and NDF content differently with increased inoculum levels. The optimal situations were 108 inoculum level for higher CP, A104 or B104 for higher EE, B106 for lower CF, B106 or B108 for lower NDF, 106 inoculum level or B strain for lower ADF. B strain at 106 inoculation level can be preferred to obtain an average CP and EE content and lower CF, NDF, and ADF content.

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep

This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 ± 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry mater intake and the output of dry mater faces 1, 2, 4, 6, 8, 10, 12, 14 days after fistulated surgery. The collection of rumen fluid was arranged at 1d during rumen surgery (DRS1), 3d after rumen surgery (ARS3), and 14d after rumen surgery (ARS14) for volatile fatty acid (VFA) and DNA extraction for sequencing and bioinformatics analysis. There was no difference in DMI, the pH apparent digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber both before and 14 days after surgery. Increases were observed in the acetate and total VFA at ARS3. There was no difference in digestion of dry material, organic material, neutral detergent fiber, and acid detergent fiber before and after surgery. The relative abundance of Bacteroides decreased from 61.96% at DRS1 to 28.85% at ARS3. In comparison with the DRS1 and ARS3, the relative abundance of Firmicutes in the ARS14 increased to 44.58% (P &lt; 0.01). Proteobacteria increased from 11.33% at DRS1 to 51.66% at ARS3 and then decreased to 11.39% at ARS14. Prevotella decreased form 61.06% at DRS1 to 28.04% in the ARS3. Succinivibrio increased from 8.32% at DRS1 to 48.58% at ARS3, but decreased to 10.43% in the ARS14. Compared with DRS1 and ARS3, the ARS14 was higher in the Simpson and Shannon index. As for the BugBase function prediction, rumen fistula surgery increased the microorganism abundance of aerobic and facultative anaerobic phenotype, and anaerobic phenotype was decreased in the ARS3. There was higher microorganism abundance of aerobic phenotype in the ARS14 than before fistula installation. In conclusion, the rumen fistula surgery destroys the anaerobic environment of rumen, leading to differences in rumen microbial diversity and function, but the apparent digestibility and total VFA were not affected.

Yield and forage quality in maize (Zea mays L.) inbred lines

Objective: To analyze the genetic diversity (agronomic attributes, forage yield, and fiber content) of 100 maize inbred lines and to identify genotypes with potential for the breeding of plants with the said traits. Design/methodology/approach: One-hundred maize inbred lines were evaluated in two environments, in a 10 x 10 complete block experimental design, with two repetitions. Days to male flowering, days to female flowering, ear height, plant height, stem diameter, green forage yield, neutral detergent fiber, and acid detergent fiber data were recorded. Results: Significant differences were observed in the environmental sources of variation and genotypes for all the traits evaluated. The first four components account for 81% of the total variation observed and the first two account for 66% of the variation. The variables which have the highest absolute value and which strongly influence the dispersion of the genotypes, as well as the formation of groups, were: plant height, forage yield, and acid detergent fiber (component one) and days to female flowering and days to male flowering (component two). Consequently, the genotypes were dispersed in four groups. Study limitations/implications: It was not possible to conduct genetic diversity studies using molecular markers. Findings/conclusions: The grouping pattern of inbred lines shows the genetic diversity present; this can be an important tool in genetic improvement programs aimed at obtaining hybrids with specific characteristics.

Changes in the Fermentation and Bacterial Community by Artificial Saliva pH in RUSITEC System

The purpose of the study was to assess the artificial saliva (AS) pH on ruminal fermentation and rumen bacteria community in the rumen simulation technique (RUSITEC) system. The experiment was performed in two treatments (low AS pH vs. high AS pH) with four replicates. The low AS pH was sustained by altering the composition of the AS (NaHCO3 from 9.8 to 1.96 g/L, Na2HPO4 from 9.3 to 1.86 g/L) according to McDougall's method. The diets were supplemented with 16 g basic diets with forage to the concentrate ratio of 50:50. The experiments were conducted over 13-day incubation periods, with 9 days adaption and 4 days sample collection. The results showed low AS pH decreased dry matter (DM) degradability (64.37 vs. 58.67%), organic matter (OM) degradability (64.38 vs. 59.32%), neutral detergent fiber (NDF) degradability (46.87 vs. 39.94%), acid detergent fiber (ADF) degradability (38.16 vs. 31.13%), and crude protein (CP) degradability (70.33 vs. 62.99%), respectively. Compared with the high AS pH, the low AS pH increased the proportion of butyrate (P = 0.008) and decreased the proportion of propionate (P &lt; 0.001). At the bacteria community, the low AS pH increased the abundances of Spirochaetes (P = 0.001) and Synergistetes (P = 0.004) and decreased the Verrucomicrobia abundance (P = 0.004) in solid-associated bacteria. At the genus level, the low AS pH increased the abundance of Lactobacillus (P = 0.050) and decreased the abundance of Schwartzia (P = 0.002) in solid-associated bacteria. The abundances of Prevotellaceae_YAB2003_group (P = 0.040), Schwartzia (P = 0.002), and Ruminobacter (P = 0.043) were lower in the low AS pH group compared with the high AS pH group in liquid-associated bacteria. Low AS pH decreased the number of Ruminococcus albus, Ruminococcus flavefaciens, Fibrobacter succinogenes (P &lt; 0.001) both in the solid- and liquid-associated bacteria, respectively. The results of the present study included three groups of bacteria communities according to the different sensitives to rumen pH: the abundances of Lactobacillus, Succinivibrio, and Prevotella_7 are increased with decreasing AS pH; the amounts of R. albus, R. flavefaciens, F. succinogenes as well as the abundances of Schwartzia and Ruminobacter decreased with the reducing AS pH; the abundances of Selenomonas_1, Rikenellaceae_RC9_gut_group, and Succiniclasticum were not affected by the AS pH in RUSTITEC.

Kualitas dan Nilai Nutrisi Silase Daun Sorgum Manis untuk Pakan Ternak

Biomassa sorgum manis yang berupa daun sorgum berpotensi sebagai pakan ternak yang melimpah saat panen. Pembuatan silase daun sorgum merupakan upaya mendapatkan pakan yang tersedia sepanjang musim dengan cara diawetkan melalui proses ensilasi. Tujuan penelitian ini adalah mengetahui kualitas dan nilai nutrisi silase daun sorgum manis. Pembuatan silase daun sorgum menggunakan bakteri Lactobacillus plantarum dengan perlakuan penambahan tetes dan urea. Contoh sebelum dan sesudah ensilasi dianalisis pH, acid detergent fiber (ADF), neutral detergent fiber (NDF), selulosa, lignin, kadar air, kadar N dan kadar abu. Hasil penelitian menunjukkan ensilasi dapat menurunkan pH silase daun sorgum dengan penurunan pH antara 0,13 - 1,33 poin. Kualitas silase daun sorgum tergolong sedang hingga sangat baik dengan nilai FN 56- 92. Nutrisi silase daun sorgum berdasar kandungan protein yang tersedia termasuk kelompok kualitas cukup dan sedang yaitu berkisar antara 8-12%. Nilai nutrisi silase daun sorgum berdasar kandungan serat yang sulit dicerna (ADF), total serat yang dapat dicerna (TDN) dan energi metabolisme pakan (ME) termasuk dalam kelompok kualitas baik hingga sangat baik dengan nilai ADF kurang dari 37%, TDN lebih dari 54%, dan ME lebih dari 0,94 Mkal/lb.

Evaluation of cereal forage grasses and forages from them according to the content of protein insoluble in acid detergent

The nitrogen content was determined in acid-detergent fiber (ADF) isolated from awnless rump, meadow fescue and meadow timothy, depending on the phase of their the same phases are for silage and haylage. Samples for analyzes were dried at a temperature of 60-65 ° C. As the grasses grew, an increase in the content of CDC in them was observed, which was also accompanied by an increase in ADF from the phase of entering the tube to flowering in timothy grass - from 1.18 to 1.36%, in meadow fescue - from 0.96 to 1.58 5%. When preserving grasses, an increase in the content of CDC is observed in all phases of their growth as compared to the original grass, but the mass fraction of ADF in the dry matter of silage and haylage was no more than in the original grass. If the technology of harvesting silage and haylage is observed, the level of thermal damage to these forages does not increase. With the growth of grasses from the vegetative phase to flowering, the content of SP in grasses and forages from them decreases, while the proportion of ADF in it increases. The inverse relationship between these indicators had correlation coefficients of 0.83, 0.88 and 0.92 for grasses, silage and haylage, respectively. The need to harvest them in earlier phases of growth is noted.

Evaluation of thermo-mechanical and microbial-facilitated processing on the chemical composition of soybean meal

A laboratory experiment was conducted to examine the effects of microbial fermentation using Bacillus subtilis and Aspergillus oryzae on the chemical composition of a commercial soybean meal (SBM). Five quadruplicate samples of SBM were subjected to four treatments with one batch serves as a control. The treatments were steam conditioning treatment (P1) where the other three groups were further fermented with B. subtilis (P2), A. oryzae (P3), and the combination of B. subtilis + A. oryzae (P4). The results showed that bacterial and fungal inoculation increased crude protein (CP) content when compared to control (p<0.05). In addition, fiber fractions including neutral detergent fiber (NDF) and acid detergent fiber (ADF) were concomitantly decreased with fermentation (p<0.05). In this study, no significant difference was observed on CP and NDF content with heating treatment (P1, p>0.05). However, this treatment decreased ADF content (p<0.05). Ether extract (EE), ash, non-fibrous carbohydrates (NFC), and total phosphorus contents were not affected by the treatments. To conclude, fermentation either with bacterial or fungal inoculants was effective to improve the chemical composition of SBM as indicated by increasing CP and decreasing fiber contents of SBM.