A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination

The decriminalization of cannabis and the growing interest in cannabinoids as therapeutics require efficient methods to discover novel compounds and monitor cannabinoid levels in human samples and products. However, current methods are limited by the structural diversity of the active compounds. Here, we construct a G-protein coupled receptor-based yeast whole-cell biosensor, optimize it to achieve high sensitivity and dynamic range, and prove its effectiveness in three real-life applications. First, we screen a library of compounds to discover two novel agonists and four antagonists and demonstrate that our biosensor can democratize GPCR drug discovery by enabling low-cost high-throughput analysis using open-source automation. Subsequently, we bioprospect 54 plants to discover a novel phytocannabinoid, dugesialactone. Finally, we develop a robust portable device, analyze body-fluid samples, and confidently detect illicit synthetic drugs like “Spice”/“K2”. Taking advantage of the extensive sensing repertoire of GPCRs, this technology can be extended to detect numerous other compounds.

The Place of Traditional Birth Attendants in Global Maternal Health: Policy Retreat, Ambivalence and Return

In this chapter, I tell the story of the waxing and waning of the status of the traditional birth attendant (TBA) in global maternal health policy from the launch of the Safe Motherhood Initiative in 1987 to the present. Once promoted as part of the solution to reducing maternal mortality, the training and integration of TBAs into formal healthcare systems in the global south was deemed a failure and side-lined in the late 1990s in favour of ‘a skilled attendant at every birth’. This shift in policy has been one of the core debates in the history of the global maternal health movement and TBAs continue to be regarded with deep ambivalence by many health providers, researchers and policymakers at the national and global levels. In this chapter, I take a critical global heath perspective that scrutinises the knowledge, policy and practice of global health in order to make visible the broader social, cultural and political context of its making. In this chapter, I offer a series of critiques of global maternal health policy regarding TBAs: one, that the evidence cited to underpin the policy shift was weak and inconclusive; two, that the original TBA component itself was flawed; three, that the political and economic context of the first decade of the SMI was not taken into account to explain the failure of TBAs to reduce maternal mortality; and four, that the reorganisation of the Safe Motherhood movement at the global level demanded a new humanitarian logic that had no room for the figure of the traditional birth attendant. I close the chapter by looking at the return of TBAs in global level policy, which, I argue, is bolstered by a growing evidence base, and also by the trends towards ‘self-care’ and point-of-use technologies in global health.

Reducing the Environmental Impact of Sterilization Packaging for Surgical Instruments in the Operating Room: A Comparative Life Cycle Assessment of Disposable versus Reusable Systems

The widespread use of single-use polypropylene packaging for sterilization of surgical instruments (blue wrap) results in enormous environmental pollution and plastic waste, estimated at 115 million kilograms on a yearly basis in the United States alone. Rigid sterilization containers (RSCs) are a well-known alternative in terms of quality and price. This paper deals with two research questions investigating the following aspects: (A) the environmental advantage of RCS for high volumes (5000 use cycles) in big hospitals, and (B) the environmental break-even point of use-cycles for small hospitals. An in-depth life cycle assessment was used to benchmark the two systems. As such a benchmark is influenced by the indicator system, three indicator systems were applied: (a) carbon footprint, (b) ReCiPe, and (c) eco-costs. The results are as follows: (1) the analyzed RSC has 85% less environmental impact in carbon footprint, 52% in ReCiPe, and 84.5% in eco-costs; and (2) an ecological advantage already occurs after 98, 228, and 67 out of 5000 use cycles, respectively. Given these two alternative packaging systems with comparable costs and quality, our results show that there are potentially large environmental gains to be made when RSC is preferred to blue wrap as a packaging system for sterile surgical instruments on a global scale.

Seasonal lead release to drinking water and the effect of aluminum

Monitoring lead in drinking water is important for public health, but seasonality in lead concentrations can bias monitoring programs if it is not understood and accounted for. Here, we describe an apparent seasonal pattern in lead release to orthophosphate-treated drinking water, identified through point-of-use sampling at sites in Halifax, Canada, with various sources of lead. Using a generalized additive model, we extracted the seasonally-varying components of time series representing a suite of water quality parameters and we identified aluminum as a correlate of lead. To investigate aluminum’s role in lead release, we modeled the effect of variscite (AlPO4 · 2H2O) precipitation on lead solubility, and we evaluated the effects of aluminum, temperature, and orthophosphate concentration on lead release from new lead coupons. At environmentally relevant aluminum and orthophosphate concentrations, variscite precipitation increased predicted lead solubility by decreasing available orthophosphate. Increasing the aluminum concentration from 20–500 µg L-1 increased lead release from coupons by 41% and modified the effect of orthophosphate, rendering it less effective. We attributed this to a decrease in the concentration of soluble (<0.45 µm) phosphorus with increasing aluminum and an accompanying increase in particulate lead and phosphorus (>0.45 µm).

Willingness of boarding school students in Tanzania to employ point-of-use water treatment: a case study

Access to safe drinking water is a challenge for students in primary and secondary schools in Tanzania. Only 32.7% of primary and secondary schools in Tanzania have access to safe drinking water and the point-of-use water treatment is rarely used. The traditional water disinfection method by boiling is often limited in boarding schools due to cost and time constraints. The objectives were to assess the willingness and attitude of boarding school students toward the use of the alternative water treatment method and determine the quality of drinking water before and after the introduction of the new method. Chlorine tablets were used due to their availability, ease of use, cost, and effectiveness. Weekly evaluations on usage, performance, and acceptability of chlorine tablets were assessed on 42 randomly selected students over a 3-week period in parallel with water sampling and testing before and after using chlorine tablets. Before the introduction of chlorine, only 17% of the students were aware of chlorine tablets, and water sources tested positive for fecal coliform. After introducing the new method, there was a significant increase (p&lt;0.05) in the attitude of students toward water treatment (100%), water quality (78%), and water consumption (67%). The work demonstrates the need to provide access to cost-effective household water treatment methods, especially in public schools that lack access to safe water.