Pollen morphology of Ellisiophyllum and Sibthorpia (Plantaginaceae, tribe Sibthorpieae) and phylogenetics of the tribe

Pollen morphology of six species belonging to genera Ellisiophyllum and Sibthorpia (Plantaginaceae tribe Sibthorpieae) was studied using light and scanning electron microscopy. The data were analyzed in the light of the first phylogenetic analysis including all but one species of the tribe using DNA sequence data from nuclear ribosomal (ITS) and plastid trnL-F region. Pollen grains in representatives of this tribe are 3-colpate, occasionally 3-porate, suboblate to prolate; mainly medium-sized, rarely small. One major pollen type (3-colpate) is recognized in the tribe. Within this pollen type, six subtypes are distinguished based on their exine sculpture, pollen grain size, length of the apertures, and exine thickness. The obtained results confirm that pollen characters are useful for species identification. Palynomorphological data are consistent with the results of the molecular phylogenetic analyses. All studies support a sister relationship of the widespread European Sibthorpia europaea with the widespread South American Sibthorpia repens and a sister relationship of two insular species, the Balearic Sibthorpia africana and the Madeiran Sibthorpia peregrina. Pollen grains in the tribe Sibthorpieae have both reticulate exine sculpture characteristic for representatives of the Russelieae–Cheloneae–Antirrhineae clades of Plantaginaceae, and also nanoechinate sculpture, which is typical for the Veroniceae and Plantagineae clades of that family. Also, in Sibthorpia repens, we observe a possible transition from the colpate type to the porate type typical for taxa of Plantago and Littorella.

Proximate, mineral and melissopalynological analyses of honeys produced by Apis mellifera adansonii maintained at the University of Lagos Apiary

This study focused on the management of established Apis mellifera adansonii colonies, and evaluation of the proximate, mineral, and pollen contents of honeys produced by these bees at the University of Lagos apiary. Two newly established bee colonies were managed using modern hive management techniques. Honeys produced by these colonies were subjected to further laboratory analyses. Proximate composition of honey samples was determined based on the official analysis methods from Association of Official Analytical Chemists (AOAC). Mineral composition was determined quantitatively using atomic absorption spectroscopy (AAS). Melissopalynological analysis was conducted to ascertain the amount, type and origin of pollen present in the samples. On the basis of the proximate composition, colony 1 honey had higher carbohydrate (81.29%), crude fibre (1.43%) and ash contents (0.70%) while colony 2 honey recorded higher protein (2.72%), crude fat (0.17%), moisture content (17.32%) and pH (4.6). Result of the mineral analysis showed that potassium was the most abundant element, while manganese was the least present trace element in both honeys. Investigated honey samples contained 8609 pollen and spore types belonging to 27 families and 29 species. Colony 1 honey had the highest pollen diversity, while colony 2 honey recorded the highest abundance. Hippocratea sp. was the predominant pollen type in colony 1 honey sample while Phyllantus sp. was the secondary dominant species in colony 2 honey. Celastraceae and Rubiaceae were the most dominant families recorded in the investigated honey samples. Investigated honey samples were multifloral, rich in minerals, and met the standard requirements of good honey as recommended by International Honey Commission. The study provided the basis for identification of major plants visited by Apis mellifera honeybees within the apiary. There is a need to conserve the existing flora within the study location, especially those found to be rewarding to Apis mellifera adansonii.

Key parameters for landscape evolution and anthropogenisation estimation in the Kamchia River downstream region (Eastern Bulgaria)

Pollen productivity еstimate (PPE) and relevant source area of pollen (RSAP) are critical parameters for quantitative interpretations of pollen data in palaeolandscape and palaeoecological reconstructions, and for analyses of the landscapes evolution and anthropogenisation as well. In light of this, the present paper endeavours to calculate PPE of key plant taxa and to define the RSAP in the Kamchia River Downstream Region (Eastern Bulgaria) in order to use them in landscape simulations and estimations. For the purposes of this research, a dataset of pollen counts from 10 modern pollen samples together with corresponding vegetation data, measured around each sample point in concentric rings, were collected in 2020. Three submodels of the Extended R-Value (ERV) model were used to relate pollen percentages to vegetation composition. Therewith, in order to create a calibrated model, the plant abundance of each pollen type was weighed by distance in GIS environment. The findings led to the conclusion that most of the tree taxa have PPE higher than 1 (ERV3 submodel). Cichoriceae, Fabaceae and Asteraceae have lower PPE.

Pollen morphology and its systematic value to southern South American species of Lepidaploa (Vernonieae: Asteraceae)

Palynological data have been used in Vernonieae for generic and specific delimitations, particularly in the Lepidaploinae subtribe. For this reason, pollen studies in the genus Lepidaploa are important to solve taxonomic conflicts. We characterized 23 species of Lepidaploa searching for morphological differences among themselves. We then compared the data obtained with other genera of the Lepidaploinae. The results show that the species have pollen type “C” (with polar lacuna) or “G” (without polar lacuna), oblate-spheroidal, subprolate or prolate-spheroidal, 3-colporate and equinolophate. The pollen types and shape of pollen grain are taxonomically useful. Pollen morphology is useful to distinguish species and genera of Lepidaploinae. Together with macromorphological data it is possible to delimit Lepidaploa and the species studied here.

Bioactive compounds and antioxidant potential of honey produced by africanized bees (Apis mellifera L.) in Piauí

This work evaluated the bioactive compounds and the antioxidant potential of honey produced by Africanized bees (Apis mellifera L.) in the state of Piauí, Brazil. The analyses show that all honeys investigatedpresented features of the pollen type Mimosa caesalpiniifolia. The content of phenolic compounds ranged from 27.65 to 97.01 mg in GAE g-1 of honey, with an average content of 62.66 ± 20.46 mgEAG g-1 of honey. The total flavonoid contents expressed in mg of quercentin/g of honey ranged from 5.43 to 13.02 mg of QE g-1, with an average of 8.33 ± 1.86 mg of QE g-1 of honey. The best performance of antioxidant activity by reducing power was EC50 of 0.15 and the lowest performance of antioxidant activity with IC50 was 1.24. The highest antioxidant activity (DPPH) ranged from 66.11 ± 0.02mg EAQ 100g-1, while the lowest activities were 9.16 and 10.88 mg EAQ 100g-1. The results show that the contents of total phenolic compounds, total flavonoids, DPPH antioxidant activity, and the pollen-reducing power from Piauí were influenced by botanical origin.

Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia

Jayadi LZ, Susandarini R. 2020. Melissopalynological analysis of honey produced by two species of stingless bees in Lombok Island, Indonesia. Nusantara Bioscience 12: 97-108. Honey is a natural product with a variety of benefits that commonly used as food sweeteners, health supplements, and traditional medicine. There has been no comprehensive publication regarding the diversity of pollen contained in honey produced by stingless bee Tetragonula laeviceps and Heterotrogona itama from Lombok. This study aimed to reveal the diversity of pollen content in honey through melissopalynological analysis of honey samples produced by two species of stingless bees T. laeviceps and H. itama in Lombok Island. This melissopalynological study was performed on honey samples obtained from nine villages from nine sub-districts representing the three districts on Lombok Island. Pollen extraction from honey was carried out using standard methods for melissopalynological analysis. The diversity of pollen recovered from honey varied from 15 to 41 pollen types. Total number of plant species whose pollen was recovered from honey samples was 127 which consisted of 61 families. The occurrence of predominant pollen type in particular unifloral honey samples indicated their botanical origin and presumed geographical origin of honey. Results of this study are useful in confirming botanical origin of honey and generating information on plants potential as food source for sustainable beekeeping in Lombok Island.

Flight Activities and Pollen Load of Lepidotrigona terminata Smith (Apidae: Meliponinae)

Worker bees actively forage to supply colony necessity i.e., pollens, nectar, and resin. Flight activities of the worker bees are influenced by food availability and environmental conditions. This study aimed to measure flight activities of Lepidotrigona terminata in terms of leaving and returning to the nest (including carrying of pollen and pollen type) and their relation to environmental conditions. The observation of flight activities of L. terminata were conducted from August to December 2016, at 07.00-17.00 for 1 minute with 1 hour interval. Pollen load and pollen types were analyzed by the acetolysis method. Flight activities of leaving and returning to the nest were lower at 07.00-08.00 (1 individual/min) and at 16.00-17.00 (2 individuals/min). However, the peak activities occurred at 10.00 until 13.00 (8 and 6 individuals/min for leaving and returning to the nest, respectively). Temperature and light intensity were positively correlated with flight activities of bees that carried nectar and resin, as well as leaving the nest without garbage. The returned workers averagely carried 32,696 pollen grains from four pollen types. Based on selection index values, this stingless bee species mostly preferred Araceae (ⱳi=1.522) plant family for pollen resources to others, Anacardiaceae, Aceraceae, and Acanthaceae (x2=39.32, p<0.01).