Patterns of Genetic Variation in the Eisenia nordenskioldi Complex (Oligochaeta: Lumbricidae) along an Elevation Gradient in Northern China

Eisenia nordenskioldi is the dominant earthworm species in many tundra and boreal habitats. Nothing is known about the genetic diversity of this species along the elevation gradient in China. This study sampled 28 individuals in the E. nordenskioldi complex from Wuling Mountain, northern China, to examine their external morphology and genetic diversity. Mt. Wuling is the southern limit of the distribution of the E. nordenskioldi complex. The specimens from Mt. Wuling were classified into three groups along an elevation gradient. Mismatch distribution analysis suggested that the Pleistocene glaciations possibly did not significantly affect the distribution of earthworm species in this region. We also found that elevation affected the genetic diversity, but not the external morphology of E. nordenskioldi. Given the altitudinal genetic diversity within the E. nordenskioldi complex, the phylogeography of this species provides important information for the zoogeographic reconstruction of the mountains in northern China. With the relatively limited sample size, the result is not conclusive, and further studies need to be conducted in the future to verify the results.

The myth of the universal millennial: comparing millennials’ perceptions of luxury across six countries

PurposeThe purpose of this research is to challenge the popular belief among luxury practitioners and researchers that millennials are a homogeneous and disruptive generation of consumers which is redefining luxury according to its terms.Design/methodology/approachThis study first presents comparisons of luxury perception among 1,450 actual luxury consumers between the ages of 18 and 34 years in six main luxury markets, eastern and western, mature and emerging (United States, China, Japan, Germany, France and Brazil). Within each country, millennials' perception of luxury is then compared to the perception held by previous generations (Gen X, baby boomers and seniors).FindingsThe results clearly demonstrate that millennials' definition of luxury is not internationally homogeneous; millennials do not hold a global vision that transcends frontiers. Furthermore, comparisons of luxury perceptions among nonmillennials from the same countries reveal that millennials match their national culture more than a cohesive age culture.Research limitations/implicationsThis research has two main limitations linked to the limited number of surveyed countries, along with a limited sample size of millennials per country. Nonetheless, the results give additional support to the glocalization hypothesis. Yet, as millennials represent 44% of personal luxury goods purchases, they catch attention from both luxury sellers and researchers. Evidence indicates the notion of a “millennial luxury consumer” could be still an empty label.Practical implicationsThe extensive use of the “millennial” label across countries implies generational homogeneity across borders, whereas reality is more diverse. Also despite the fact that luxury brands are highly globalized, the perception of what defines luxury – the hierarchy of its most salient attributes – does vary per country, thus needs specific attention.Originality/valueThe current findings reveal that millennials from the six surveyed countries do not share the same perceptions of luxury traits. Moreover, millennials' definition of luxury mirrors the definition held by nonmillennials from their own country, suggesting a strong cultural influence in each country.

Nailfold capillaroscopy alterations in androgenetic alopecia: A case–control study

Background: Androgenetic alopecia is considered to be an independent predictor of mortality from diabetes mellitus and heart disease. However, whether androgenetic alopecia causes changes in microcirculation is unknown. Objective: The objective of the study was to investigate whether alterations in nailfold capillaries occur in androgenetic alopecia patients. Methods: The nailfold capillaroscopy images of androgenetic alopecia patients and matched controls were collected and analyzed. Results: The frequencies of avascular areas, dilated, bushy and bizarre capillaries and capillary disorganization, nailfold capillaroscopy scores of 2 or scores both 2 and 3 were significantly higher in the androgenetic alopecia group than in the healthy controls (9.0% vs. 0%, 57.7% vs. 19.2%, 3.8% vs. 0%, 2.8% vs. 1.3%, 3.8% vs. 0%, 38.5% vs. 12.8% and 39.7% vs. 12.8%, respectively). Limitations: The results of this study may be biased on account of the limited sample size or the presence of an undiagnosed disease in participants which could alter the nailfold capillaries. Conclusion: Bushy, bizarre and dilated capillaries, capillary disorganization, avascular areas and nailfold capillaroscopy scores of 2 or 2 and 3 were more common in androgenetic alopecia patients than in healthy controls. These findings indicate that abnormalities in microcirculation may be involved in androgenetic alopecia.

Identification of Metro-Bikeshare Transfer Trip Chains by Matching Docked Bikeshare and Metro Smartcards

Metro-bikeshare integration, an important way of improving the efficiency of public transportation, has grown rapidly during the last decades in many countries. However, most previous analysis of metro-bikeshare transfer trips were based on limited sample size and the number of recognized metro-bikeshare trips were not sufficient. The primary objective of this study is to derive a method to recognize metro-bikeshare transfer trips. The two data sources are provided by Nanjing Metro Company and Nanjing Public Bicycle Company over the same period from 9–29 March 2016. The identifying method includes three steps: (1) Matching Card Pairs (2) Filtering Card Pairs and (3) Identifying Card Pairs. The case study indicates that the Support Vector Classification (SVC) performs best with a high prediction accuracy of 95.9% using seamless smartcards. The identifying method is then used to recognize the transfer trips from other types of cards, resulting in 17,022 valid metro-bikeshare transfer trips made by 2948 travelers. Finally, travel patterns extracted from the two groups of identified transfer trips are analyzed comparatively. The method proposed presents new opportunities for analyzing metro-bikeshare transfer trip characteristics.

Photometric Properties of L and T Dwarf Binaries

Recent studies using volume-limited samples of brown dwarfs have revealed the photometric evolution of single L and T dwarfs and unresolved binaries as they age and cool, in particular as they transition from L to T spectral types. We demonstrate that the near-infrared photometric evolution of the resolved components of L and T dwarf binaries is consistent with that of single objects using a volume-limited sample. In addition, we provide supporting evidence that the L9 dwarf WISE J185101.83+593508.6 is an unresolved binary and identify three more candidate or confirmed unresolved binaries all from being overluminous for their spectral type. We calculate a new binary fraction of 13.1% ± 1.8% for L0–T8 dwarfs.

Proteomic Analysis of Tears and Conjunctival Cells Collected with Schirmer Strips Using timsTOF Pro: Preanalytical Considerations

This study aimed to investigate the human proteome profile of samples collected from whole (W) Schirmer strips (ScS) and their two parts—the bulb (B) and the rest of the strip (R)—with a comprehensive proteomic approach using a trapped ion mobility mass spectrometer, the timsTOF Pro. Eight ScS were collected from two healthy subjects at four different visits to be separated into three batches, i.e., 4W, 4B, and 4R. In total, 1582 proteins were identified in the W, B, and R batches. Among all identified proteins, binding proteins (43.4%) and those with catalytic activity (42.2%) constituted more than 80% of the molecular functions. The most represented biological processes were cellular processes (31.2%), metabolic processes (20.8%), and biological regulation (13.1%). Enzymes were the most represented protein class (41%), consisting mainly of hydrolases (47.5%), oxidoreductases (22.1%), and transferases (16.7%). The bulb (B), which is in contact with the conjunctiva, might collect both tear and cell proteins and therefore promote the identification of more proteins. Processing B and R separately before mass spectrometry (MS) analysis, combined with the high data acquisition speed and the addition of ion-mobility-based separation in the timsTOF Pro, can bring a new dimension to biomarker investigations of a limited sample such as tear fluid.

Water droplet-in-oil digestion method for single-cell proteomics

Recent advances in single-cell proteomics highlight the promise of sensitive analyses in limited cell populations. However, technical challenges remain for sample recovery, throughput, and versatility. Here, we first report a water droplet-in-oil digestion (WinO) method based on carboxyl-coated beads and phase transfer surfactants for proteomic analysis using limited sample amounts. This method was developed to minimize the contact area between the sample solution and the container to reduce the loss of proteins and peptides by adsorption. This method increased protein and peptide recovery 10-fold as well as the number of quantified transmembrane proteins compared to an in-solution digestion (ISD) method. The proteome profiles obtained from 100 cells using the WinO method highly correlated with those from 10000 cells using the ISD method. We successfully applied the WinO method to single-cell proteomics and quantified 462 proteins. Using the WinO method, samples can be easily prepared in a multi-well plate, making it a widely applicable and suitable method for single-cell proteomics.

Two Massive Jupiters in Eccentric Orbits from the TESS Full-frame Images

We report the discovery of two short-period massive giant planets from NASA’s Transiting Exoplanet Survey Satellite (TESS). Both systems, TOI-558 (TIC 207110080) and TOI-559 (TIC 209459275), were identified from the 30 minute cadence full-frame images and confirmed using ground-based photometric and spectroscopic follow-up observations from TESS’s follow-up observing program working group. We find that TOI-558 b, which transits an F-dwarf (M * = 1.349 − 0.065 + 0.064 M ⊙, R * = 1.496 − 0.040 + 0.042 R ⊙, T eff = 6466 − 93 + 95 K, age 1.79 − 0.73 + 0.91 Gyr) with an orbital period of 14.574 days, has a mass of 3.61 ± 0.15 M J, a radius of 1.086 − 0.038 + 0.041 R J, and an eccentric (e = 0.300 − 0.020 + 0.022 ) orbit. TOI-559 b transits a G dwarf (M * = 1.026 ± 0.057 M ⊙, R * = 1.233 − 0.026 + 0.028 R ⊙, T eff = 5925 − 76 + 85 K, age 6.8 − 2.0 + 2.5 Gyr) in an eccentric (e = 0.151 ± 0.011) 6.984 days orbit with a mass of 6.01 − 0.23 + 0.24 M J and a radius of 1.091 − 0.025 + 0.028 R J. Our spectroscopic follow up also reveals a long-term radial velocity trend for TOI-559, indicating a long-period companion. The statistically significant orbital eccentricity measured for each system suggests that these planets migrated to their current location through dynamical interactions. Interestingly, both planets are also massive (>3 M J), adding to the population of massive giant planets identified by TESS. Prompted by these new detections of high-mass planets, we analyzed the known mass distribution of hot and warm Jupiters but find no significant evidence for multiple populations. TESS should provide a near magnitude-limited sample of transiting hot Jupiters, allowing for future detailed population studies.

Molecular Biomarkers for Adrenoleukodystrophy: An Unmet Need

X-linked adrenoleukodystrophy (ALD) is an inherited progressive neurometabolic disease caused by mutations in the ABCD1 gene and the accumulation of very long-chain fatty acids in plasma and tissues. Patients present with heterogeneous clinical manifestations which can include adrenal insufficiency, myelopathy, and/or cerebral demyelination. In the absence of a genotype-phenotype correlation, the clinical outcome of an individual cannot be predicted and currently there are no molecular markers available to quantify disease severity. Therefore, there is an unmet clinical need for sensitive biomarkers to monitor and/or predict disease progression and evaluate therapy efficacy. The increasing amount of biological sample repositories (‘biobanking’) as well as the introduction of newborn screening creates a unique opportunity for identification and evaluation of new or existing biomarkers. Here we summarize and review the many studies that have been performed to identify and improve knowledge surrounding candidate molecular biomarkers for ALD. We also highlight several shortcomings of ALD biomarker studies, which often include a limited sample size, no collection of longitudinal data, and no validation of findings in an external cohort. Nonetheless, these studies have generated a list of interesting biomarker candidates and this review aspires to direct future biomarker research.