LoG and Structural Based Arbitrary Oriented Multilingual Text Detection in Images/Video

Text in an image or a video affords more precise meaning and text is a prominent source with a clear explanation of the content than any other high-level or low-level features. The text detection process is a still challenging research work in the field of computer vision. However, complex background and orientation of the text leads to extremely stimulating text detection tasks. Multilingual text consists of different geometrical shapes than a single language. In this article, a simple and yet effective approach is presented to detect the text from an arbitrary oriented multilingual image and video. The proposed method employs the Laplacian of Gaussian to identify the potential text information. The double line structure analysis is applied to extract the true text candidates. The proposed method is evaluated on five datasets: Hua's, arbitrarily oriented, multi-script robust reading competition (MRRC), MSRA and video datasets with performance measures precision, recall and f-measure. The proposed method is also tested on real-time video, and the result is promising and encouraging.

Lab build Fully Automated microfluduic flow injection System

A fully automated microfluidic Flow Injection spectrophotometric system was designed in our laboratory with methylene blue used as an example. The device consists of a double-line microfluidic chip with dimensions (30 μl x 4 cm), each 15 μl volume intended to allow a very small volume of reagent and sample to be used. The microfluidic system also consists of two types of Arduino software. The first was the UNO of a type used to control the two mini peristaltic pumps to drive the water and methylene blue as carrier stream and sample respectively. The Mega type, which was equipped with homemade software, signal-to-peak, was the second Arduino which used as a data logger to record the results in the form of peaks using Microsoft Excel 2016. The peak’s height was corresponding to concentration. The linearity was in the range (0.025-0.125 μg/ml) with a five-point regression coefficient (0.9981), RSD% for ten replicates is 0.025 μg/mL. The detection limit was (0.001 μg/ml) and the sample throughput was 300 samples per hour and each sample required 25 μl of chemical reagents. Therefore, 240 samples need only (5.0 ml) from the chemical reagents, which clearly indicates that the consumed reagent and the waste were very low. Therefore, the method for determining methylene blue with a fully automated system is very environmentally friendly. save time, consumption regent, Improve quality, decrease cost-consumption reducing the consume of sample and inherited the reproducibility and accurate from flow injection analysis.

Acclimatization of transformed seaweed Kappaphycus alvarezii carrying lysozyme gene in culture flask and the cultivation in floating net cage in Pangkep coastal

The in vitro transformation of the lysozyme gene in seaweed K. alvarezii has been successfully executed to increase the viability against ice-ice disease. There were two major stages in this research; (1) transformation of lysozyme gene in seaweed K. alvarezii which was carried out on laboratory scale and the cultivation of gene-transformed explants in the culture flask stored in “culture chamber”; (2) the acclimatization in floating net cages of green nets (mesh size of 1 mm) with cage size of 50 x 50 x 50 cm, the population density of 200 explants and cultivated for two weeks. The explants were then transferred to blue nets (mesh size of 2 mm) with a cage size of 50 x 50 x 50 cm for four weeks of rearing. The plants were then enlarged using a long-line method in the floating net cage, by tying the seaweed using a double line with a gap of 15 cm each. The measurement of weight, bud lengths, and water quality was carried out within 2 weeks. The result shows that the daily growth rate of the transformed seaweed during the regeneration stage in the culture flask was around 0.33-0.4%/day, meanwhile during the acclimatization stage in the green nets the was 0.65-1.6%/day, and even more, increased during the acclimatization stage in the blue nets with DGR of 2.28-2.3%/day. During the enlargement stage in the floating net cages, the lysozyme-transformed seaweed showed an even higher DGR with a value of 3.2-8.2%/day. The results of the integration of the lysozyme gene in seaweed were indicated by the presence of a 670 bp of amplification products, that is the same total length of the 35 S-F promoter fragments and Nos T-R in the expression vector. Based on these results, the lysozyme gene was successfully transformed in K. alvarezii seaweed.