preclinical testing
Recently Published Documents


TOTAL DOCUMENTS

549
(FIVE YEARS 116)

H-INDEX

44
(FIVE YEARS 5)

mBio ◽  
2022 ◽  
Author(s):  
Geraldine Vilmen ◽  
Anna C. Smith ◽  
Hector Cervera Benet ◽  
Rajni Kant Shukla ◽  
Ross C. Larue ◽  
...  

Rhesus macaques are a critical animal model for preclinical testing of HIV-1 vaccine and prevention approaches. However, HIV-1 does not replicate in rhesus macaques, and thus, chimeric simian-human immunodeficiency viruses (SHIVs), which encode HIV-1 envelope glycoproteins (Envs), are used as surrogate challenge viruses to infect rhesus macaques for modeling HIV-1 infection.


2021 ◽  
Vol 2 (4) ◽  
pp. 100942
Author(s):  
Nan Li ◽  
Rosa Nguyen ◽  
Carol J. Thiele ◽  
Mitchell Ho

2021 ◽  
Vol 2 (4) ◽  
pp. 101017
Author(s):  
Silvia Pascual-Sabater ◽  
Giulia Raimondi ◽  
Ana Mato-Berciano ◽  
Eva C. Vaquero ◽  
Fabio Ausania ◽  
...  

Cancers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 5962
Author(s):  
Chiao-Yun Lin ◽  
Ren-Chin Wu ◽  
Chen-Yang Huang ◽  
Chyong-Huey Lai ◽  
An-Shine Chao ◽  
...  

Conventional treatment of dedifferentiated endometrial carcinoma (DEC)–an uncommon and highly aggressive uterine malignancy–is beset by high failure rates. A line of research that holds promise to overcome these limitations is tailored treatments targeted on specific molecular alterations. However, suitable preclinical platforms to allow a reliable implementation of this approach are still lacking. Here, we developed a patient-derived xenograft (PDX) model for preclinical testing of investigational drugs informed by molecular data. The model–termed PDX-mLung was established in mice implanted with lung metastatic lesions obtained from a patient with DEC. Histologic and whole-exome genetic analyses revealed a high degree of identity between PDX-mLung and the patient’s parental lesions (both primary DEC and lung metastases). Interestingly, molecular analyses revealed that PDX-mLung harbored druggable alterations including a FGFR2 mutation and CCNE2 amplification. Targeted combined treatment with the FGFR inhibitor lenvatinib and the cell cycle inhibitor palbociclib was found to exert synergistic therapeutic effects against in vivo tumor growth. Based on the results of RNA sequencing, lenvatinib and palbociclib were found to exert anti-tumor effects by interfering interferon signaling and activating hormonal pathways, respectively. Collectively, these data provide proof-of-concept evidence on the value of PDX models for preclinical testing of molecularly informed drug therapy in difficult-to-treat human malignancies. Further clinical research is needed to examine more rigorously the potential usefulness of the lenvatinib and palbociclib combination in patients with DEC.


2021 ◽  
Vol 6 (2) ◽  
pp. 368-378
Author(s):  
Alfian Syarifuddin ◽  
◽  
Rizka Amalia

The use of plants for traditional medicines are community tradition in Indonesia. However, the use of plants for hereditary treatment has not been well documented. One of the solutions is an ethnomedicine study, in this study 5 villages located in Secang District, Magelang Regency. The sampling technique used is snowball sampling with observation using instruments and interviews. The number of respondents sample is 388 respondents. The results showed that the community utilized 96 plant species from 47 families. There are 3 types of diseases that use plants in their treatment with an ICF value of 1, namely sprains, nosebleeds, and menstrual flow. The most widely used parts for treatment are leaves (42.61%). The most frequently used processing method is the boiling technique with a percentage of 65.10% by way of use, which is drinking 77.05%. People most often consume with a frequency of 2x a day (74.87%). People usually use treatment during illness/ curative (76.74%). People get plants from their own yard (54.24%). Knowledge about treatment comes from family (hereditary) (72.43%). From some of the results, it can be used as a reference when conducting scientific verification of preclinical testing on test animals.


2021 ◽  
Vol 141 (10) ◽  
pp. S212
Author(s):  
E. Hofmann ◽  
J.C. Holzer-Geißler ◽  
K. Tiffner ◽  
A. Eberl ◽  
T. Birngruber ◽  
...  

2021 ◽  
Vol 9 (5) ◽  
Author(s):  
Susana B. Gorzalczany ◽  
Angeles G. Rodriguez Basso
Keyword(s):  

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1279
Author(s):  
Anna Glöckner ◽  
Susann Ossmann ◽  
Andre Ginther ◽  
Jagdip Kang ◽  
Michael A. Borger ◽  
...  

Cardioplegic solutions play a major role in cardiac surgery due to the fact that they create a silent operating field and protect the myocardium against ischemia and reperfusion injury. For studies on cardioplegic solutions, it is important to compare their effects and to have a valid platform for preclinical testing of new cardioplegic solutions and their additives. Due to the strong anatomical and physiological cardiovascular similarities between pigs and humans, porcine models are suitable for investigating the effects of cardioplegic solutions. This review provides an overview of the results of the application of cardioplegic solutions in adult or pediatric pig models over the past 25 years. The advantages, disadvantages, limitations, and refinement strategies of these models are discussed.


mBio ◽  
2021 ◽  
Author(s):  
Jesús Horacio Lara-Puente ◽  
Juan Manuel Carreño ◽  
Weina Sun ◽  
Alejandro Suárez-Martínez ◽  
Luis Ramírez-Martínez ◽  
...  

Several highly efficacious vaccines for SARS-CoV-2 have been developed and are used in the population. However, the current production capacity cannot meet the global demand. Therefore, additional vaccines—especially ones that can be produced locally and at low cost—are urgently needed. This work describes preclinical testing of a SARS-CoV-2 vaccine candidate which meets these criteria.


Sign in / Sign up

Export Citation Format

Share Document