Histological Feature of Uterus and Uterine Tube During Late Period of Pregnancy in Rabbits

Current work was conducted to investigate the histological architecture of the uterine tube and uterus during the period of late pregnancy in rabbits. Twelve adult local breed rabbits were used. The samples from different parts of the uterine tube were prepared for histological study after staining with H&E, Masson’s trichrome and combine Alcian blue (pH2.5)-PAS. The pre ampulla was a narrow tube and its tunica mucosa had slightly long simple mucosal folds lined by pseudostratified columnar epithelium, the ampulla had a wide lumen and its tunica mucosa displayed several highly tall branched mucosal folds with few short simple folds while the isthmus was the narrowest region and its tunica mucosa displayed few of tall and short simple mucosal folds. The mucosa of three parts of the uterine tube was lined by pseudostratified columnar epithelium which is composed of three types of cells: mucous secretory cells, non-secretory cells and basal cells, the mucous secretory cells were the predominant type and revealed secretory activities. The lamina propria-submucosa of the uterine tube was composed of cellular connective tissue and tunica muscularis. The uterus had a very thick wall with well-recognized endometrium and myometrium, the endometrium was composed of many-branched and simple endometrial folds that were covered by multinucleated syncytial cells and simple cuboidal epithelial cells. Lamina propria was composed of loose connective tissue had scattered groups of well growing simple uterine glands that showed secretory activities. The current result showed that the uterus during the late period of pregnancy was differed from those of non-pregnant rabbits, while the uterine tube at late pregnancy has a structure that appeared prepared for the next physiological period.

MORPHOMETRIC CHARACTERISTICS OF ANGIOARCHITECTONICS OF THE NORMAL UTERINE TUBE MUCOUS MEMBRANE AND UNDER THE EFFECT OF OPIOID DURING THE EXPERIMENT

Introduction. In the modern world, the problem of drug use, especially among the youth, is considered to be of utmost importance. Female body’s susceptibility to the development of drug addiction is considerably higher than that of the male body. The study of blood supply of the uterine tube is relevant since the morphological rebuilding of the mucous membrane of the uterine tube, considering its functions, plays an important role in the pathogenesis of several diseases of the female reproductive system. Under the effect of pathological factors, including opioids, vessels became the main target, and their structural changes are the basis for the development of the pathological process and determine the peculiarities of clinical symptoms. Methods. The study was carried out on 34 sexually mature white female rats (4,5-6,0 months) with the bodyweight of 180-220 g. Angioarchitectonics of the uterine tube of female white rats was studied in double-blinded, placebo-controlled studies with distribution into a control group (intact rats that were accepted as a relative norm, n=10), a placebo-controlled group (n=9) that received 0.9% saline for the duration of the experiment, and 3 experimental groups (n=5 each) that were subjected to long-term opioid effect. Results. After 2 weeks of Nalbuphine administration for female white laboratory rat, the minor expansion of arterioles and capillaries was observed, their diameter increased. Arteriolovenular coefficient augmented. After 4 weeks of Nalbuphine administration, arterioles were convoluted, loss of clear outlines, expansion of lumen, irregularity of vessels caliber, microvessels with microaneurysms, areas with hemorrhage and obliteration of capillaries were detected. After six-week administration of nalbuphine, the hemomicrocirculatory blood flow of the uterine tube wall was at the stage of decompensation, the capillary component was almost destroyed, arterioles were abruptly twisted and distorted, their lumen was uneven, venules were expanded and distorted, additional arteriolovenular anastomoses were opened, indices of trophic activity of the tissue increased considerably. Conclusions. The vasotropic effect after six-week administration of nalbuphine demonstrated severe damage to the uterine tube hemomicrocirculatory blood flow.

Morphological characteristics of uterine tubes ampullae in elderly females

The aim of the study is to determine the size and to identify the features of histoarchitectonics of the uterine tube ampulla of an old-aged female. Materials and methods. The ampulla of both uterine tubes was examined in 26 nulliparous and 78 giving birth elderly women using morphometric, histological, immunohistochemical, and micrometric research methods. Results. The length and outer diameter of uterine tubes at the points of transition of isthmus into the ampulla and ampulla into the infundibulum as well as in the central part of ampulla are characterized by the absence of a statistically significant difference in parameters in nulliparous and giving birth women. The histoarchitectonic features of the ampullae of the uterine tubes in nulliparous and giving birth women in old age consist in the flattening of the epithelium of the mucous membrane, which forms many closely spaced thickened folds that create an uneven narrowing of the ampulla lumen, and the proliferation of connective tissue between the bundles of muscle fibers of the muscular membrane. The presence of Ki-67 expression in single cells of less than 1%, as well as a thin layer of epithelial membrane antigen in the epithelium of the ampulla along with the complete absence of its expression in the stroma reflect normal tissue regeneration and act as an equivalent to the anatomical norm in elderly females. When comparing lumen areas of ampulla and its wall at mid-section as well as the circumference of epithelial lining, a predominance of parameters in the right uterine tube was revealed (p<0.01). Conclusion. The study showed the similar qualitative and quantitative morphological characteristics of the uterine tube ampullae both in nulliparous and giving birth females in old age.

ACCORDING TO THE ACCUMULATION OF LIQUID IN THE UTERINE TUBE. SEVERAL ANIMAL EXPERIENCES

Diseases of the fallopian tubes occupy a prominent place in modern gynecology, and among these diseases, serious attention is paid clinically to the accumulation of fluid in the tube cavity. Professor K.F. Slavyanskiy in his lectures, read in November 1890 at the clinic of the Imperial Military Medical Academy, which he headed, examined in detail the issue of retention cysts of the fallopian tubes and under this name grouped diseases known as hydrosalpinx, pyosalpinx, haematosalpinx, cystes tubo-ovariales.

Primary multiple BRCA associated breast cancer and ovarian cancer (clinical case)

Introduction. The study of mutation in BRCA1/2 genes was first initiated in the USA and Europe, and later in Russia. Statistics indicate that women with the BRCA1/BRCA2 mutation have a higher risk of breast and/or ovarian cancer than the general population. According to different authors, the average cumulative risk among BRCA1 carriers is 65% (range 44–78%) for breast cancer and 39% (range 18–54%) for ovarian cancer. For mutation carriers in the BRCA2 gene, the risk for breast cancer is 45–49%, while the risk for RNA is 11–18%. However, in patients already diagnosed with breast cancer or ovarian cancer, the risk of a second tumor persists throughout life and may remain high even in old age. Treatment of BRCA-associated breast cancer and/or ovarian cancer is almost the same as treatment for sporadic cancer, and includes surgical, radiation, and drug anticancer therapy. However, there are some features that need to be considered in clinical practice. Clinical case. In this article we present the clinical experience of the treatment of a 32-year-old patient with BRCA1-associated primary multiple synchronous breast cancer and metachronous uterine tube cancer. In July 2015, the patient was diagnosed with synchronous cancer of both breast (Luminal A right breast cancer and Luminal B left breast cancer). As part of a treatment and with the patient’s consent, a bilateral adnexectomy was performed. In the histological examination of the operating material, the uterine tube cancer was diagnosed in situ. From 16.03.2016 to the present time the patient receives adjuvant endocrinotherapy without signs of disease progression. Conclusion. This clinical case study presents the importance of a combined approach to the treatment and prevention of BRCAassociated cancer.

Histomorphological features of Rabbit Uterine Tube During Estrous Phases

Microscopic features of the adult rabbit uterine tube during estrous cycle were studied. Twenty rabbit uterine tube were used. Rabbits were collected from local market. Sections from uterine tube infundibulum, ampulla and isthmus were prepared for histological study. Lengths and widths of various parts were obtained from the right tube. Ampulla formed the longest part, isthmus was narrowest segment and connected to the uterus. The infundibulum was with fimbrae. Epithelial liming was with few types of cells, and were; ciliated; non-ciliated secretory (peg cells) and basal cells were also demonstrated. The type of epithelial cells was pseudostratified epithelium. In the ampullary mucosa, large number of primary branches at the follicular phase was observed. Ciliation was more at the follicular phase than luteal phase. While secretory cells during follicular was less than that at luteal phase http://dx.doi.org/10.25130/tjps.24.2019.062

Characteristics of the uterine tubes in the fetal period: topographic and morphometric parallels

Topicality of the study is stipulated by the importance of objective data for medicine concerning formation of the structure and topographic-anatomical interrelations of the uterine tubes with adjacent organs and structures during the prenatal period of human ontogenesis. Objective: to find and compare tendencies of changes of the uterine tube morphologic parameters in the two groups of fetuses remote in time, and determine age peculiarities of their topography during perinatal period of development. The experimental material (specimens of fetuses) was divided into two groups: І group – 35 specimens of fetuses deceased during 2017-2019; ІІ group – 105 specimens of fetuses taken from the Museum of the Departments of Anatomy, Clinical Anatomy and Operative Surgery at Higher State Educational Establishment of Ukraine “Bukovinian State Medical University”, collected during 1970-1990. Every group was subdivided into 7 subgroups according to 10 months of the fetal period of development (from the 4th to the 10th months). The results obtained were statistically processed in the licensed statistical package “Statistica 6.0” using nonparametric methods to evaluate the results. Regular changes in the topography of the right and left uterine tubes, changes in their shape and histological structure are observed. Both uterine tubes were found to occupy an ascending position in thirty cases out of sixty examined specimens of early fetuses (4-6 months of age). In twenty cases one uterine tube was found to be in an ascending position, and in ten cases both uterine tubes were placed practically horizontally. The study of uterine tube morphogenesis in 7-month fetuses found that the structure and topography of the uterine tubes in different age periods differ. In two 7-month fetuses the uterine tubes were found to be in an ascending position, in eight cases one uterine tube (the left one – in six cases out of eight) was in a horizontal position, and in ten cases two uterine tubes were directed practically horizontally. During the 8th month of the antenatal development one uterine tube was found to be in an ascending position out of eight cases; in ten cases both uterine tubes were in the position close to a horizontal one; and in two cases the left uterine tube was in a descending position. In 9-month fetuses both uterine tubes were found to be in a horizontal position in twelve cases, in eight cases one of the uterine tubes was in the position close to a descending one. In fourteen fetuses 10 months of development one uterine tube was in a descending position, and only in six cases both uterine tubes were located horizontally. The length of the uterine tubes of 4-7-month fetuses deceased during 2017-2019 was not found to differ reliably. Similar regularity was found in the group of fetuses aged from 9 to 10 months. The length of the uterine tubes of the archival specimens increases reliably every two months. In this group of fetuses the parameters of the uterine tube length aged from 9 to 10 months were found to differ reliably contrary to the length of the uterine tubes in the group of modern specimens of a similar age. Comparison of dynamics in changes of the uterine tube length in the two groups of fetuses remote in time showed that within the frame of one group differences in the morphometric parameters between the right and left uterine tubes are not considerable. Therefore, the study of peculiarities in the structure of the uterine tubes at every stage of the perinatal period found certain peculiarities and regularities of their development.